{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T20:32:22Z","timestamp":1788640342778,"version":"build-2803163510"},"reference-count":28,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,15]],"date-time":"2021-05-15T00:00:00Z","timestamp":1621036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2019R1A6A1A03033553"],"award-info":[{"award-number":["2019R1A6A1A03033553"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018532","name":"Major Scientific and Technological Innovation Project of Shandong Province","doi-asserted-by":"publisher","award":["2019JZZY020613"],"award-info":[{"award-number":["2019JZZY020613"]}],"id":[{"id":"10.13039\/501100018532","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>In the present study, the in vitro and in vivo anti-inflammatory effects of the sulfated polysaccharides isolated from Sargassum fulvellum (SFPS) were evaluated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and zebrafish. The results indicated that SFPS improved the viability of LPS-stimulated RAW 264.7 macrophages from 80.02 to 86.80, 90.09, and 94.62% at the concentration of 25, 50, and 100 \u00b5g\/mL, respectively. Also, SFPS remarkably and concentration-dependently decreased the production levels of inflammatory molecules including nitric oxide (NO), tumor necrosis factor-alpha, prostaglandin E2, interleukin-1 beta, and interleukin-6 in LPS-treated RAW 264.7 macrophages. In addition, SFPS significantly inhibited the expression levels of cyclooxygenase-2 and inducible nitric oxide synthase in LPS-treated RAW 264.7 macrophages. Furthermore, the in vivo test results indicated that SFPS improved the survival rate of LPS-treated zebrafish from 53.33 to 56.67, 60.00, and 70.00% at the concentration of 25, 50, and 100 \u00b5g\/mL, respectively. In addition, SFPS effectively reduced cell death, reactive oxygen species, and NO levels in LPS-stimulated zebrafish. Taken together, these results suggested that SFPS possesses strong in vitro and in vivo anti-inflammatory activities, and could be used as an ingredient to develop anti-inflammatory agents in the functional food and pharmaceutical industries.<\/jats:p>","DOI":"10.3390\/md19050277","type":"journal-article","created":{"date-parts":[[2021,5,16]],"date-time":"2021-05-16T23:17:16Z","timestamp":1621207036000},"page":"277","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["In Vitro and In Vivo Anti-Inflammatory Effects of Sulfated Polysaccharides Isolated from the Edible Brown Seaweed, Sargassum fulvellum"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1477-2725","authenticated-orcid":false,"given":"Lei","family":"Wang","sequence":"first","affiliation":[{"name":"College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye-Won","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1666-6048","authenticated-orcid":false,"given":"Ginnae","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Marine Bio Food Science, Chonnam National University, Yeosu 59626, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0038-1459","authenticated-orcid":false,"given":"Xiaoting","family":"Fu","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiachao","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3299-7266","authenticated-orcid":false,"given":"You-Jin","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea"},{"name":"Marine Science Institute, Jeju National University, Jeju 63333, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1016\/j.ijbiomac.2017.09.050","article-title":"In vitro and in vivo anti-inflammatory activities of high molecular weight sulfated polysaccharide; containing fucose separated from Sargassum horneri: Short com-munication","volume":"107","author":"Sanjeewa","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"de Souza Costa, M., de Brito, T.V., de Oliveira, S.B., de Souza Brauna, I., Neto, J.C.R.M., Teles, R.H.G., Dutra, Y.M., de Aguiar Magalh\u00e3es, D., Sousa, S.G., and de Sousa, J.A. (2021). Photobiomodulation exerts anti-inflammatory effects on the vascular and cellular phases of experimental inflammatory models. Lasers Med. Sci.","DOI":"10.1007\/s10103-021-03298-x"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.ijbiomac.2019.09.128","article-title":"Anti-inflammatory and anti-melanogenesis activities of sulfated polysaccharides isolated from Hizikia fusiforme: Short communication","volume":"142","author":"Wang","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3","DOI":"10.4162\/nrp.2017.11.1.3","article-title":"Anti-inflammatory activity of a sulfated polysac-charide isolated from an enzymatic digest of brown seaweed Sargassum horneri in RAW 264.7 cells","volume":"11","author":"Sanjeewa","year":"2017","journal-title":"Nutr. Res. Pract."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Wang, L., Oh, J.Y., Hwang, J., Ko, J.Y., Jeon, Y.-J., and Ryu, B. (2019). In Vitro and In Vivo Antioxidant Activities of Polysaccharides Iso-lated from Celluclast-Assisted Extract of an Edible Brown Seaweed, Sargassum fulvellum. Antioxidants, 8.","DOI":"10.3390\/antiox8100493"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"101761","DOI":"10.1016\/j.algal.2019.101761","article-title":"The potential nutritive value of Sargassum fulvellum as a feed ingredient for ruminants","volume":"45","author":"Choi","year":"2020","journal-title":"Algal Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.jep.2007.10.032","article-title":"Antipyretic, analgesic, and anti-inflammatory activities of the seaweed Sargassum fulvellum and Sargassum thunbergii in mice","volume":"116","author":"Kang","year":"2008","journal-title":"J. Ethnopharmacol."},{"key":"ref_8","first-page":"1","article-title":"Pharmaceutical and Nutraceutical Potential Applications of Sargassum fulvellum","volume":"2020","author":"Liu","year":"2020","journal-title":"BioMed Res. Int."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.intimp.2016.07.015","article-title":"In vivo and in vitro inhibitory activity of an ethanolic extract of Sargassum fulvellum and its component grasshopper ketone on atopic dermatitis","volume":"40","author":"Kang","year":"2016","journal-title":"Int. Immunopharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"820","DOI":"10.4014\/jmb.1901.01027","article-title":"Anti-Inflammatory Effects of Grasshopper Ketone from Sargassum ful-vellum Ethanol Extract on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Cells","volume":"29","author":"Kim","year":"2019","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"113","DOI":"10.17642\/jcc.23.2.7","article-title":"Protective Effects of Enzyme-assistant Extracts of Sargassum fulvellum against AAPH-induced Oxidative Stress in vitro in Vero Cells","volume":"23","author":"Wang","year":"2018","journal-title":"J. Chitin Chitosan"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1016\/j.ijbiomac.2020.11.116","article-title":"Sulfated polysaccharides from Undaria pinnatifida im-proved high fat diet-induced metabolic syndrome, gut microbiota dysbiosis and inflammation in BALB\/c mice","volume":"167","author":"Jiang","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Apostolova, E., Lukova, P., Baldzhieva, A., Katsarov, P., Nikolova, M., Iliev, I., Peychev, L., Trica, B., Oancea, F., and Delattre, C. (2020). Immunomodulatory and Anti-Inflammatory Effects of Fucoidan: A Review. Polymers, 12.","DOI":"10.3390\/polym12102338"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Fitton, J.H., Park, A.Y., Karpiniec, S.S., and Stringer, D.N. (2020). Fucoidan and Lung Function: Value in Viral Infection. Mar. Drugs, 19.","DOI":"10.3390\/md19010004"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Mabate, B., Daub, C.D., Malgas, S., Edkins, A.L., and Pletschke, B.I. (2021). Fucoidan Structure and Its Impact on Glucose Metabolism: Implications for Diabetes and Cancer Therapy. Mar. Drugs, 19.","DOI":"10.3390\/md19010030"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Pozharitskaya, O.N., Obluchinskaya, E.D., and Shikov, A.N. (2020). Mechanisms of Bioactivities of Fucoidan from the Brown Sea-weed Fucus vesiculosus L. of the Barents Sea. Mar. Drugs, 18.","DOI":"10.3390\/md18050275"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"112157","DOI":"10.1016\/j.fct.2021.112157","article-title":"Structural characteristics and an-ti-inflammatory activity of UV\/H2O2-treated algal sulfated polysaccharide from Gracilaria lemaneiformis","volume":"152","author":"Gong","year":"2021","journal-title":"Food Chem. Toxicol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1016\/j.ijbiomac.2017.07.031","article-title":"A fucoidan fraction purified from Chnoospora minima; a potential inhibitor of LPS-induced inflammatory responses","volume":"104","author":"Fernando","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/j.ijbiomac.2020.04.012","article-title":"In vitro and in vivo anti-inflammatory activities of a fucose-rich fucoidan isolated from Saccharina japonica","volume":"156","author":"Ni","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"115195","DOI":"10.1016\/j.carbpol.2019.115195","article-title":"Fucoidan isolated from Padina commersonii inhibit LPS-induced inflammation in macrophages blocking TLR\/NF-\u03baB signal pathway","volume":"224","author":"Jayawardena","year":"2019","journal-title":"Carbohydr. Polym."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"114030","DOI":"10.1016\/j.jep.2021.114030","article-title":"Anti-inflammatory effect of leaves of Vernonia zeylanica in lipopolysaccharide-stimulated RAW 264.7 macrophages and carrageenan-induced rat paw-edema model","volume":"274","author":"Rukshala","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_22","first-page":"328","article-title":"Fucoidan isolated from the popular edible brown seaweed Sar-gassum fusiforme suppresses lipopolysaccharide-induced inflammation by blocking NF-\u03baB signal pathway","volume":"18","author":"Wang","year":"2021","journal-title":"J. Appl. Phycol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"429","DOI":"10.3892\/etm.2021.9846","article-title":"Anti-inflammatory effects of Fritillaria thunbergii Miquel extracts in LPS-stimulated murine macrophage RAW 264.7 cells","volume":"21","author":"Kim","year":"2021","journal-title":"Exp. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1016\/j.fct.2010.05.003","article-title":"Evaluation of anti-inflammatory effect of fucoxanthin isolated from brown algae in lipopolysaccharide-stimulated RAW 264.7 macrophages","volume":"48","author":"Heo","year":"2010","journal-title":"Food Chem. Toxicol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.fsi.2017.07.041","article-title":"The roles of NF-\u03baB and ROS in regulation of pro-inflammatory mediators of inflammation induction in LPS-stimulated zebrafish embryos","volume":"68","author":"Ko","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1021\/acssuschemeng.9b05290","article-title":"Characterization and Application of Lignin\u2013Carbohydrate Complexes from Lignocellulosic Materials as Antioxidants for Scavenging In Vitro and In Vivo Reactive Oxygen Species","volume":"8","author":"Dong","year":"2019","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.ijbiomac.2021.01.103","article-title":"Evaluating the bio-application of biomacro-molecule of lignin-carbohydrate complexes (LCC) from wheat straw in bone metabolism via ROS scavenging","volume":"176","author":"Zheng","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.carbpol.2013.11.022","article-title":"Protective effect of fucoidan against AAPH-induced oxidative stress in zebrafish model","volume":"102","author":"Kim","year":"2014","journal-title":"Carbohydr. Polym."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/19\/5\/277\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:02:06Z","timestamp":1760162526000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/19\/5\/277"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,15]]},"references-count":28,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5]]}},"alternative-id":["md19050277"],"URL":"https:\/\/doi.org\/10.3390\/md19050277","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,15]]}}}