{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T02:56:24Z","timestamp":1785466584334,"version":"3.56.0"},"reference-count":69,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T00:00:00Z","timestamp":1634256000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The Hantaan orthohantavirus (genovariant Amur\u2013AMRV) is a rodent-borne zoonotic virus; it is the causative agent of haemorrhagic fever with renal syndrome in humans. The currently limited therapeutic options require the development of effective anti-orthohantavirus drugs. The ability of native fucoidan from Fucus evanescens (FeF) and its enzymatically prepared high-molecular-weight (FeHMP) and low-molecular-weight (FeLMP) fractions to inhibit different stages of AMRV infection in Vero cells was studied. The structures of derivatives obtained were determined using nuclear magnetic resonance (NMR) spectroscopy. We found that fucoidan and its derivatives exhibited significant antiviral activity by affecting the early stages of the AMRV lifecycle, notably virus attachment and penetration. The FeHMP and FeLMP fractions showed the highest anti-adsorption activity by inhibiting AMRV focus formation, with a selective index (SI) &gt; 110; FeF had an SI of ~70. The FeLMP fraction showed a greater virucidal effect compared with FeF and the FeHMP fraction. It was shown by molecular docking that 2O-sulphated fucotetrasaccharide, a main component of the FeLMP fraction, is able to bind with the AMRV envelope glycoproteins Gn\/Gc and with integrin \u03b23 to prevent virus\u2013cell interactions. The relatively small size of these sites of interactions explains the higher anti-AMRV activity of the FeLMP fraction.<\/jats:p>","DOI":"10.3390\/md19100577","type":"journal-article","created":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T13:35:47Z","timestamp":1634304947000},"page":"577","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["In Vitro Anti-Orthohantavirus Activity of the High-and Low-Molecular-Weight Fractions of Fucoidan from the Brown Alga Fucus evanescens"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9048-6803","authenticated-orcid":false,"given":"Natalia V.","family":"Krylova","sequence":"first","affiliation":[{"name":"G.P. Somov Institute of Epidemiology and Microbiology, Rospotrebnadzor, Selskaya Street, 1, 690087 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artem S.","family":"Silchenko","sequence":"additional","affiliation":[{"name":"G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, Prospect 100 let Vladivostoku, 159, 690022 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia B.","family":"Pott","sequence":"additional","affiliation":[{"name":"G.P. Somov Institute of Epidemiology and Microbiology, Rospotrebnadzor, Selskaya Street, 1, 690087 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana P.","family":"Ermakova","sequence":"additional","affiliation":[{"name":"G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, Prospect 100 let Vladivostoku, 159, 690022 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6723-582X","authenticated-orcid":false,"given":"Olga V.","family":"Iunikhina","sequence":"additional","affiliation":[{"name":"G.P. Somov Institute of Epidemiology and Microbiology, Rospotrebnadzor, Selskaya Street, 1, 690087 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton B.","family":"Rasin","sequence":"additional","affiliation":[{"name":"G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, Prospect 100 let Vladivostoku, 159, 690022 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7315-6119","authenticated-orcid":false,"given":"Galina G.","family":"Kompanets","sequence":"additional","affiliation":[{"name":"G.P. Somov Institute of Epidemiology and Microbiology, Rospotrebnadzor, Selskaya Street, 1, 690087 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1937-1680","authenticated-orcid":false,"given":"Galina N.","family":"Likhatskaya","sequence":"additional","affiliation":[{"name":"G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, Prospect 100 let Vladivostoku, 159, 690022 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail Y.","family":"Shchelkanov","sequence":"additional","affiliation":[{"name":"G.P. Somov Institute of Epidemiology and Microbiology, Rospotrebnadzor, Selskaya Street, 1, 690087 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s10393-017-1305-2","article-title":"Global Diversity and Distribution of Hantaviruses and Their Hosts","volume":"15","author":"Milholland","year":"2018","journal-title":"EcoHealth"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Kabwe, E., Davidyuk, Y., Shamsutdinov, A., Garanina, E., Martynov, E., Kitaeva, K., Malisheni, M., Isaeva, G., Savitskaya, T., and Urbanowicz, R.A. (2020). Orthohantaviruses, Emerging Zoonotic Pathogens. Pathogens, 9.","DOI":"10.3390\/pathogens9090775"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e6","DOI":"10.1111\/1469-0691.12291","article-title":"Hantavirus infections","volume":"21","author":"Saksida","year":"2019","journal-title":"Clin. Microbiol. Inf."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.3389\/fmicb.2019.02989","article-title":"Vaccines and therapeutics against Hantaviruses","volume":"10","author":"Liu","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"925","DOI":"10.3389\/fbioe.2020.00925","article-title":"Biodefense Implications of New-World Hantaviruses","volume":"8","author":"Patel","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.3201\/eid2512.181649","article-title":"Hemorrhagic Fever with Renal Syndrome, Russia","volume":"25","author":"Tkachenko","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_7","first-page":"10","article-title":"Epidemiological and epizootological characteristics of foci with a group incidence of hemorrhagic fever with renal syndrome in the Primorsky Territory","volume":"3","author":"Slonova","year":"2013","journal-title":"Epidemiol. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"620279","DOI":"10.3389\/fpubh.2021.620279","article-title":"Hemorrhagic Fever with Renal Syndrome in Vladivostok City, Russia","volume":"9","author":"Yashina","year":"2021","journal-title":"Front. Public Health"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3","DOI":"10.4149\/av_2017_01_3","article-title":"Antiviral therapy and prevention against hantavirus infections","volume":"61","author":"Szabo","year":"2017","journal-title":"Acta Virol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.antiviral.2008.09.007","article-title":"Experience with intravenous ribavirin in the treatment of hemorrhagic fever with renal syndrome in Korea","volume":"81","author":"Rusnak","year":"2009","journal-title":"Antivir. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1080\/23744235.2017.1293841","article-title":"Insufficient efficacy and safety of intravenous ribavirin in treatment of haemorrhagic fever with renal syndrome caused by Puumala virus","volume":"49","author":"Malinin","year":"2017","journal-title":"Infect. Dis."},{"key":"ref_12","first-page":"846","article-title":"Fucoidans-sulfated polysaccharides of brown algae","volume":"78","author":"Usov","year":"2009","journal-title":"Success Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1016\/j.carbpol.2013.10.019","article-title":"The system of fucoidans from the brown seaweed Dictyota dichotoma: Chemical analysis and antiviral activity","volume":"101","author":"Rabanal","year":"2014","journal-title":"Carbohydr. Polym."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Cunha, L., and Grenha, A. (2016). Sulfated Seaweed Polysaccharides as Multifunctional Materials in Drug Delivery Applications. Mar. Drugs, 14.","DOI":"10.3390\/md14030042"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Wang, Y., Xing, M., Cao, Q., Ji, A., Liang, H., and Song, S. (2019). Biological Activities of Fucoidan and the Factors Mediating Its Therapeutic Effects: A Review of Recent Studies. Mar. Drugs, 17.","DOI":"10.3390\/md17030183"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1093\/glycob\/cwm014","article-title":"A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds","volume":"17","author":"Cumashi","year":"2007","journal-title":"Glycobiology"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Li, B., Lu, F., Wei, X.J., and Zhao, R.X. (2008). Fucoidan: Structure and bioactivity. Molecules, 13.","DOI":"10.3390\/molecules13081671"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1134\/S1063074009020084","article-title":"Comparative study of chemical composition and antitumor activity of aqueous-ethanol extracts of brown algae Laminaria cichorioides, Costaria costata, and Fucus evanescens","volume":"35","author":"Imbs","year":"2009","journal-title":"Russ. J. Mar. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2106","DOI":"10.3390\/md9102106","article-title":"Important determinants for fucoidan bioactivity: A critical review of structure-function relations and extraction methods for fucose-containing sulfated polysaccharides from brown seaweeds","volume":"9","author":"Ale","year":"2011","journal-title":"Mar. Drugs"},{"key":"ref_20","first-page":"254","article-title":"Expression and biochemical characterization and substrate specificity of the fucoidanase from Formosa algae","volume":"27","author":"Silchenko","year":"2017","journal-title":"Glycobiology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"116635","DOI":"10.1016\/j.carbpol.2020.116635","article-title":"Enzymatic transformation and anti-tumor activity of Sargassum horneri fucoidan","volume":"246","author":"Rasin","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1093\/glycob\/cwl029","article-title":"Cloning and biochemical characterization of the fucanase FcnA: Definition of a novel glycoside hydrolase family specific for sulfated fucans","volume":"16","author":"Colin","year":"2006","journal-title":"Glycobiology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.3389\/fmicb.2020.01674","article-title":"Discovery and Characterization of an Endo-1,3-Fucanase From Marine Bacterium Wenyingzhuangia fucanilytica: A Novel Glycoside Hydrolase Family","volume":"11","author":"Shen","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.carbpol.2018.03.094","article-title":"Modification of native fucoidan from Fucus evanescens by recombinant fucoidanase from marine bacteria Formosa algae","volume":"193","author":"Silchenko","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Silchenko, A.S., Rasin, A.B., Zueva, A.O., Kusaykin, M.I., Zvyagintseva, T.N., Kalinovsky, A.I., Kurilenko, V.V., and Ermakova, S.P. (2018). Fucoidan Sulfatases from Marine Bacterium Wenyingzhuangia fucanilytica CZ1127T. Biomolecules, 8.","DOI":"10.3390\/biom8040098"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Krylova, N.V., Ermakova, S.P., Lavrov, V.F., Leneva, I.A., Kompanets, G.G., Iunikhina, O.V., Nosik, M.N., Ebralidze, L.K., Falynskova, I.N., and Silchenko, A.S. (2020). The Comparative Analysis of Antiviral Activity of Native and Modified Fucoidans from Brown Algae Fucus evanescens In Vitro and In Vivo. Mar. Drugs, 18.","DOI":"10.3390\/md18040224"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1007\/s007050070077","article-title":"In vitro antiviral activity of lactoferrin and ribavirin upon hantavirus","volume":"145","author":"Murphy","year":"2000","journal-title":"Arch. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Safronetz, D., Haddock, E., Feldmann, F., Ebihara, H., and Feldmann, H. (2011). In Vitro and In Vivo Activity of Ribavirin against Andes Virus Infection. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0023560"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2704","DOI":"10.3390\/v5112704","article-title":"Ribavirin Protects Syrian Hamsters against Lethal Hantavirus Pulmonary Syndrome\u2014After Intranasal Exposure to Andes Virus","volume":"5","author":"Ogg","year":"2013","journal-title":"Viruses"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"7074","DOI":"10.1073\/pnas.95.12.7074","article-title":"beta3 Integrins mediate the cellular entry of hantaviruses that cause respiratory failure","volume":"95","author":"Gavrilovskaya","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"7319","DOI":"10.1128\/JVI.79.12.7319-7326.2005","article-title":"Peptide antagonists that inhibit Sin Nombre virus and hantaan virus entry through the beta3-integrin receptor","volume":"79","author":"Larson","year":"2005","journal-title":"J. Virol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1111\/j.1747-0285.2007.00489.x","article-title":"Characterization and NMR solution structure of a novel cyclic pentapeptide inhibitor of pathogenic hantaviruses","volume":"69","author":"Hall","year":"2007","journal-title":"Chem. Biol. Drug Des."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/j.celrep.2016.03.082","article-title":"A Molecular-Level Account of the Antigenic Hantaviral Surface","volume":"15","author":"Li","year":"2016","journal-title":"Cell Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e0253120","DOI":"10.1128\/mBio.02531-20","article-title":"Structural Basis for a Neutralizing Antibody Response Elicited by a Recombinant Hantaan Virus Gn Immunogen","volume":"6","author":"Rissanen","year":"2021","journal-title":"mBio"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2595","DOI":"10.1099\/vir.0.045302-0","article-title":"Identification of a novel B-cell epitope of Hantaan virus glycoprotein recognized by neutralizing 3D8 monoclonal antibody","volume":"93","author":"Yan","year":"2012","journal-title":"J. Gen. Virol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1248\/cpb.52.1091","article-title":"Novel Antiviral Fucoidan from Sporophyll of Undaria pinnatifida (Mekabu)","volume":"52","author":"Lee","year":"2004","journal-title":"Chem. Pharm. Bull."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.intimp.2007.10.017","article-title":"Defensive effects of a fucoidan from brown alga Undaria pinnatifida against herpes simplex virus infection","volume":"8","author":"Hayashi","year":"2008","journal-title":"Int. Immunopharmacol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.bbrc.2008.08.100","article-title":"Structure and anti-dengue virus activity of sulfated polysaccharide from a marine alga","volume":"376","author":"Hidari","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.carbpol.2014.08.068","article-title":"Anti-HIV activity of fucoidans from three brown seaweed specie","volume":"115","author":"Thuy","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"40760","DOI":"10.1038\/srep40760","article-title":"Inhibition of influenza A virus infection by fucoidan targeting viral neuraminidase and cellular EGFR pathway","volume":"7","author":"Wang","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_41","first-page":"86","article-title":"Antiviral activity of naturally occurring fucoidans in experimental infection caused by Hantavirus","volume":"2","author":"Maksema","year":"2008","journal-title":"Pac. Med. J."},{"key":"ref_42","first-page":"29","article-title":"Screening of biopolymers from sea hydrocoles affecting the adsorption of Hantaan virus","volume":"52","author":"Makarenkova","year":"2007","journal-title":"Virus Res."},{"key":"ref_43","first-page":"12","article-title":"Inhibiting effects of fucoidans on Hantaan virus adsorption on a model of peritoneal macrophages in vitro","volume":"53","author":"Makarenkova","year":"2008","journal-title":"Virus Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1007\/s12560-016-9233-9","article-title":"The Experimental Research (In Vitro) of Carrageenans and Fucoidans to Decrease Activity of Hantavirus","volume":"8","author":"Pavliga","year":"2016","journal-title":"Food Environ. Virol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.carbpol.2019.01.105","article-title":"Fucoidan production: Approval key challenges and opportunities","volume":"211","author":"Zayed","year":"2019","journal-title":"Carbohydr. Polym."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1248\/cpb.49.484","article-title":"A novel antivirally active fucan sulfate derived from an edible brown alga, Sargassum horneri","volume":"49","author":"Preeprame","year":"2001","journal-title":"Chem. Pharm. Bull."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"27239","DOI":"10.1074\/jbc.M401774200","article-title":"A New Model for the Domain Structure of Heparan Sulfate Based on the Novel Specificity of K5 Lyase","volume":"279","author":"Murphy","year":"2004","journal-title":"JBC"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"14785","DOI":"10.1038\/s41598-017-15009-0","article-title":"Sequencing of glycosaminoglycans with potential to interrogate sequence-specific interactions","volume":"7","author":"Oosterhof","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1093\/glycob\/cwn092","article-title":"Focus on antivirally active sulfated polysaccharides: From structure\u2013activity analysis to clinical evaluation","volume":"19","author":"Ghosh","year":"2009","journal-title":"Glycobiology"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Lin, Z., Tan, X., Zhang, Y., Li, F., Ping, L., and Liu, H. (2020). Molecular Targets and Related Biologic Activities of Fucoidan: A Review. Mar. Drugs, 18.","DOI":"10.3390\/md18080376"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.3390\/v6041801","article-title":"Hantavirus Gn and Gc Envelope Glycoproteins: Key Structural Units for Virus Cell Entry and Virus Assembly","volume":"6","author":"Tischler","year":"2014","journal-title":"Viruses"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"859","DOI":"10.2217\/fvl.15.52","article-title":"Bunyaviruses: From transmission by arthropods to virus entry into the mammalian host first-target cells","volume":"10","author":"Leger","year":"2015","journal-title":"Future Virol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/bs.aivir.2019.07.002","article-title":"Hantavirus entry: Perspectives and recent advances","volume":"104","author":"Mittler","year":"2019","journal-title":"Adv. Virus Res."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Ermonval, M., Baychelier, F., and Tordo, N. (2016). What Do We Know about How Hantaviruses Interact with Their Different Hosts?. Viruses, 8.","DOI":"10.3390\/v8080223"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3951","DOI":"10.1128\/JVI.73.5.3951-3959.1999","article-title":"Cellular entry of hantaviruses which cause hemorrhagic fever with renal syndrome is mediated by beta3 integrins","volume":"73","author":"Gavrilovskaya","year":"1999","journal-title":"J. Virol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Popugaeva, E., Witkowski, P.T., Schlegel, M., Ulrich, R.G., Auste, B., Rang, A., Kr\u00fcger, D.H., and Klempa, B. (2012). Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0035587"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"5270","DOI":"10.4049\/jimmunol.169.9.5270","article-title":"CD11b\/CD18-dependent interactions of neutrophils with intestinal epithelium are mediated by fucosylated proteoglycans","volume":"169","author":"Zen","year":"2002","journal-title":"J. Immunol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4777","DOI":"10.7150\/jca.26740","article-title":"Antimetastatic Effect of Fucoidan-Sargassum against Liver Cancer Cell Invadopodia Formation via Targeting Integrin \u03b1V\u03b23 and Mediating \u03b1V\u03b23\/Src\/E2F1 Signaling","volume":"10","author":"Pan","year":"2019","journal-title":"J. Cancer"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1186\/1743-422X-9-307","article-title":"In vitro characterization of the antiviral activity of fucoidan from Cladosiphon okamuranus against Newcastle Disease Virus","volume":"9","year":"2012","journal-title":"Virol. J."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"768","DOI":"10.3201\/eid0808.010494","article-title":"Genetic Characterization of Hantaviruses Transmitted by the Korean Field Mouse (Apodemus peninsulae), Far East Russia","volume":"8","author":"Lokugamage","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/S0008-6215(99)00206-2","article-title":"A new procedure for the separation of water-soluble polysaccharides from brown seaweeds","volume":"322","author":"Zvyagintseva","year":"1999","journal-title":"Carbohydr. Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1134\/S1068162007010116","article-title":"Polysaccharide and lipid composition of the brown seaweed Laminaria gurjanovae","volume":"33","author":"Shevchenko","year":"2007","journal-title":"Rus. J. Bioorg. Chem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3025","DOI":"10.1016\/j.ijbiomac.2020.08.131","article-title":"Expression and biochemical characterization of two recombinant fucoidanases from the marine bacterium Wenyingzhuangia fucanilytica CZ1127T","volume":"164","author":"Zueva","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1042\/bj0780312","article-title":"Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate esters","volume":"78","author":"Dodgson","year":"1961","journal-title":"Biochem. J."},{"key":"ref_65","first-page":"18","article-title":"Replication features of hantaviruses\u2014Causative agents Hemorrhagic Fever With Renal Syndrome in cell cultures of different origins","volume":"19","author":"Malkin","year":"2014","journal-title":"Epidemiol. Infect. Dis."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1038\/s41598-018-37446-1","article-title":"Orthohantaviruses belonging to three phylogroups all inhibit apoptosis in infected target cells","volume":"9","author":"Gupta","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.cell.2020.08.023","article-title":"The Hantavirus Surface Glycoprotein Lattice and Its Fusion Control Mechanism","volume":"183","author":"Serris","year":"2020","journal-title":"Cell"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"40252","DOI":"10.1074\/jbc.C400362200","article-title":"A novel adaptation of the integrin PSI domain revealed from its crystal structure","volume":"279","author":"Xiong","year":"2004","journal-title":"J. Biol. 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