{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T14:47:16Z","timestamp":1790952436753,"version":"4.1.0"},"reference-count":42,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education","award":["2021R1A6A1A03039211"],"award-info":[{"award-number":["2021R1A6A1A03039211"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Recent studies have revealed that marine brown seaweeds contain numerous bioactive compounds which exhibit various bioactivities. The present study investigated the effect of low molecular weight fucoidan (SCF) isolated from Sargassum confusum, a brown alga, on inflammatory responses and oxidative stress in HaCaT keratinocytes stimulated by tumor necrosis factor (TNF)-\u03b1\/interferon (IFN)-\u03b3. SCF significantly increased the cell viability while decreasing the intracellular reactive oxygen species (ROS) production in TNF-\u03b1\/IFN-\u03b3-stimulated HaCaT keratinocytes. In addition, SCF effectively reduced inflammatory cytokines (interleukin (IL)-1\u03b2, IL-6, IL-8, IL-13, TNF-\u03b1, and IFN-\u03b3) and chemokines (Eotaxin, macrophage-derived chemokine (MDC), regulated on activation, normal T cell expressed and secreted (RANTES), and thymus and activation-regulated chemokine (TARC)) expression, by down-regulating the expression of epithelial and epidermal innate cytokines (IL-25, IL-33, and thymic stromal lymphopoietin (TSLP)). Furthermore, SCF suppressed the activation of TNF-\u03b1\/IFN-\u03b3-stimulated mitogen-activated protein kinase (MAPK) and nuclear factor-\u03baB (NF-\u03baB) signaling pathways, while activating the nuclear factor erythroid 2-related factor 2 (Nrf2)\/heme oxygenase-1 (HO-1) signaling pathway. The cytoprotective effect of SCF against TNF-\u03b1\/IFN-\u03b3 stimulation was considerably reduced upon inhibition of HO-1 activity by ZnPP. Overall, these results suggest that SCF effectively suppressed inflammatory responses and oxidative stress in TNF-\u03b1\/IFN-\u03b3-stimulated HaCaT keratinocytes via activating the Nrf2\/HO-1 signaling pathway.<\/jats:p>","DOI":"10.3390\/md20020117","type":"journal-article","created":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T22:16:18Z","timestamp":1643753778000},"page":"117","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Fucoidan Isolated from Sargassum confusum Suppresses Inflammatory Responses and Oxidative Stress in TNF-\u03b1\/IFN-\u03b3- Stimulated HaCaT Keratinocytes by Activating Nrf2\/HO-1 Signaling Pathway"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3943-2452","authenticated-orcid":false,"given":"Arachchige Maheshika Kumari","family":"Jayasinghe","sequence":"first","affiliation":[{"name":"Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9364-3037","authenticated-orcid":false,"given":"Kirinde Gedara Isuru Sandanuwan","family":"Kirindage","sequence":"additional","affiliation":[{"name":"Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9450-4239","authenticated-orcid":false,"given":"Ilekuttige Priyan Shanura","family":"Fernando","sequence":"additional","affiliation":[{"name":"Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eui Jeong","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Korea"},{"name":"Research Center for Healthcare and Biomedical Engineering, Chonnam National University, Yeosu 59626, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gun-Woo","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, 45, Yongso-ro, Nam-gu, Busan 48513, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1615-750X","authenticated-orcid":false,"given":"Won-Kyo","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, 45, Yongso-ro, Nam-gu, Busan 48513, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ginnae","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Korea"},{"name":"Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"106146","DOI":"10.1016\/j.intimp.2019.106146","article-title":"Eckol from Ecklonia cava ameliorates TNF-\u03b1\/IFN-\u03b3-induced inflammatory responses via regulating MAPKs and NF-\u03baB signaling pathway in HaCaT cells","volume":"82","author":"Cho","year":"2020","journal-title":"Int. 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