{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T12:19:33Z","timestamp":1774009173603,"version":"3.50.1"},"reference-count":41,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,2,4]],"date-time":"2022-02-04T00:00:00Z","timestamp":1643932800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"publisher","award":["20170488"],"award-info":[{"award-number":["20170488"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Melanin synthesis is a defense mechanism that prevents skin damage, but excessive accumulation of melanin occurs in the skin in various reactions such as pigmentation, lentigines, and freckles. Although anti-melanogenic effects have been demonstrated for various naturally occurring marine products that inhibit and control tyrosinase activity, most studies have not been extended to in vivo applications. Phlorofucofuroeckol-A (PFF-A, 12.5\u2013100 \u00b5M) isolated from Ecklonia cava has previously been shown to have tyrosinase-mitigative effects in B16F10 cells, but it has not been evaluated in an in vivo model, and its underlying mechanism for anti-melanogenic effects has not been studied. In the present study, we evaluated the safety and efficacy of PFF-A for anti-melanogenic effects in an in vivo model. We selected low doses of PFF-A (1.5\u201315 nM) and investigated their mitigative effects on pigmentation stimulated by \u03b1-MSH in vivo and their related-mechanism in an in vitro model. The findings suggest that low-dose PFF-A derived from E. cava suppresses pigmentation in vivo and melanogenesis in vitro. Therefore, this study presents the possibility that PFF-A could be utilized as a new anti-melanogenic agent in the cosmeceutical industries.<\/jats:p>","DOI":"10.3390\/md20020123","type":"journal-article","created":{"date-parts":[[2022,2,4]],"date-time":"2022-02-04T11:35:17Z","timestamp":1643974517000},"page":"123","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Mitigative Effects of PFF-A Isolated from Ecklonia cava on Pigmentation in a Zebrafish Model and Melanogenesis in B16F10 Cells"],"prefix":"10.3390","volume":"20","author":[{"given":"Jun-Geon","family":"Je","sequence":"first","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunfei","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Food Engineering, Jilin Agriculture Science and Technology University, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun-Ho","family":"Heo","sequence":"additional","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xining","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Northeast Normal University, Changchun 130024, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3299-7266","authenticated-orcid":false,"given":"You-Jin","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea"},{"name":"Marine Science Institute, Jeju National University, Jeju 63333, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5797-500X","authenticated-orcid":false,"given":"Bo-Mi","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s00403-014-1489-z","article-title":"Melanogenesis inhibitory effect of aerial part of Pueraria thunbergiana in vitro and in vivo","volume":"307","author":"Han","year":"2015","journal-title":"Arch. 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