{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T19:10:59Z","timestamp":1790795459072,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["PE99922"],"award-info":[{"award-number":["PE99922"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007049","name":"Korea Institute of Ocean Science and Technology","doi-asserted-by":"publisher","award":["PE99922"],"award-info":[{"award-number":["PE99922"]}],"id":[{"id":"10.13039\/501100007049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>In this study, we investigated the anti-allergic effects of 3,4-dihydroxybenzaldehyde (DHB) isolated from the marine red alga, Polysiphonia morrowii, in mouse bone-marrow-derived cultured mast cells (BMCMCs) and passive cutaneous anaphylaxis (PCA) in anti-dinitrophenyl (DNP) immunoglobulin E (IgE)-sensitized mice. DHB inhibited IgE\/bovine serum albumin (BSA)-induced BMCMCs degranulation by reducing the release of \u03b2-hexosaminidase without inducing cytotoxicity. Further, DHB dose-dependently decreased the IgE binding and high-affinity IgE receptor (Fc\u03b5RI) expression and Fc\u03b5RI-IgE binding on the surface of BMCMCs. Moreover, DHB suppressed the secretion and\/or the expression of the allergic cytokines, interleukin (IL)-4, IL-5, IL-6, IL-13, and tumor necrosis factor (TNF)-\u03b1, and the chemokine, thymus activation-regulated chemokine (TARC), by regulating the phosphorylation of I\u03baB\u03b1 and the translocation of cytoplasmic NF-\u03baB into the nucleus. Furthermore, DHB attenuated the passive cutaneous anaphylactic (PCA) reaction reducing the exuded Evans blue amount in the mouse ear stimulated by IgE\/BSA. These results suggest that DHB is a potential therapeutic candidate for the prevention and treatment of type I allergic disorders.<\/jats:p>","DOI":"10.3390\/md20020133","type":"journal-article","created":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T02:35:51Z","timestamp":1644546951000},"page":"133","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Anti-Allergic Effect of 3,4-Dihydroxybenzaldehyde Isolated from Polysiphonia morrowii in IgE\/BSA-Stimulated Mast Cells and a Passive Cutaneous Anaphylaxis Mouse Model"],"prefix":"10.3390","volume":"20","author":[{"given":"Eun-A","family":"Kim","sequence":"first","affiliation":[{"name":"Jeju Marine Research Center, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eui-Jeong","family":"Han","sequence":"additional","affiliation":[{"name":"Research Center for Healthcare and Biomedical Engineering, Chonnam National University, Yeosu 59626, Korea"},{"name":"Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junseong","family":"Kim","sequence":"additional","affiliation":[{"name":"Jeju Marine Research Center, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilekuttige Priyan Shanura","family":"Fernando","sequence":"additional","affiliation":[{"name":"Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Young","family":"Oh","sequence":"additional","affiliation":[{"name":"Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7712-7697","authenticated-orcid":false,"given":"Kil-Nam","family":"Kim","sequence":"additional","affiliation":[{"name":"Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ginnae","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Korea"},{"name":"Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo-Jin","family":"Heo","sequence":"additional","affiliation":[{"name":"Jeju Marine Research Center, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, Korea"},{"name":"Department of Marine Biology, University of Science and Technology, Daejeon 34113, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s12940-020-0563-6","article-title":"Association between exposure to traffic-related air pollution and pediatric allergic diseases based on modeled air pollution concentrations and traffic measures in Seoul, Korea: A comparative analysis","volume":"19","author":"Min","year":"2020","journal-title":"Environ. Health"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1007\/s43440-019-00048-3","article-title":"Inhibitory effects of orientin in mast cell-mediated allergic inflammation","volume":"72","author":"Dhakal","year":"2020","journal-title":"Pharmacol. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1007\/s12016-011-8252-4","article-title":"IgE, Mast Cells, and Eosinophils in Atopic Dermatitis","volume":"41","author":"Liu","year":"2011","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1016\/S1074-7613(01)00159-5","article-title":"Monomeric IgE Stimulates Signaling Pathways in Mast Cells that Lead to Cytokine Production and Cell Survival","volume":"14","author":"Kalesnikoff","year":"2001","journal-title":"Immunity"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"112484","DOI":"10.1016\/j.jep.2019.112484","article-title":"Prunus serrulata var. spontanea inhibits mast cell activation and mast cell-mediated anaphylaxis","volume":"250","author":"Kim","year":"2019","journal-title":"J. Ethnopharmacol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"11895","DOI":"10.1038\/s41598-017-12252-3","article-title":"Polydatin inhibits mast cell-mediated allergic inflammation by targeting PI3K\/Akt, MAPK, NF-\u03baB and Nrf2\/HO\u22121 pathways","volume":"7","author":"Ye","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1111\/exd.12814","article-title":"Dieckol, a phlorotannin ofEcklonia cava, suppresses IgE-mediated mast cell activation and passive cutaneous anaphylactic reaction","volume":"24","author":"Ahn","year":"2015","journal-title":"Exp. Dermatol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"61","DOI":"10.4168\/aair.2017.9.1.61","article-title":"Inhibition of allergic response by intranasal selective NF-\u03baB decoy oligodeoxynucleotides in a murine model of allergic rhinitis","volume":"9","author":"Wee","year":"2017","journal-title":"Allergy Asthma Immunol. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"114087","DOI":"10.1016\/j.bcp.2020.114087","article-title":"5-Bromo-3,4-dihydroxybenzaldehyde from Polysiphonia morrowii attenuate IgE\/BSA-stimulated mast cell activation and passive cutaneous anaphylaxis in mice","volume":"178","author":"Han","year":"2020","journal-title":"Biochem. Pharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Han, E.J., Kim, H.-S., Sanjeewa, K., Herath, K., Jeon, Y.-J., Jee, Y., Lee, J., Kim, T., Shim, S.-Y., and Ahn, G. (2020). Eckol from Ecklonia cava Suppresses Immunoglobulin E-mediated Mast Cell Activation and Passive Cutaneous Anaphylaxis in Mice (Running Title: Anti-Allergic Activity of Ecklonia cava). Nutrients, 12.","DOI":"10.3390\/nu12051361"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"S359","DOI":"10.4103\/0973-1296.210173","article-title":"Peimine inhibits the production of proinflammatory cytokines through regulation of the phosphorylation of NF-\u03baB and MAPKs in HMC-1 Cells","volume":"13","author":"Park","year":"2017","journal-title":"Pharmacogn. Mag."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"99","DOI":"10.17642\/jcc.24.2.4","article-title":"Anti-allergic Effect of Methanol Extract from Polysiphonia morrowii Harveyin IgE\/BSA-stimulated Bone Marrow-derived Cultured Mast Cells anda Passive Cutaneous Anaphylaxis Mouse Model","volume":"24","author":"Han","year":"2019","journal-title":"J. Chitin Chitosan"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.intimp.2018.11.021","article-title":"3-Bromo-5-(ethoxymethyl)-1, 2-benzenediol inhibits LPS-induced pro-inflammatory responses by preventing ROS production and downregulating NF-\u03baB in vitro and in a zebrafish model","volume":"67","author":"Ko","year":"2019","journal-title":"Int. Immunopharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.4014\/jmb.1904.04062","article-title":"Protective Effect of 3-Bromo-4,5-Dihydroxybenzaldehyde from Polysiphonia morrowii Harvey against Hydrogen Peroxide-Induced Oxidative Stress In Vitro and In Vivo","volume":"29","author":"Cho","year":"2019","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1002\/ptr.6266","article-title":"Bromophenol (5-bromo-3,4-dihydroxybenzaldehyde) isolated from red alga Polysiphonia morrowii inhibits adipogenesis by regulating expression of adipogenic transcription factors and AMP-activated protein kinase activation in 3T3-L1 adipocytes","volume":"33","author":"Ko","year":"2018","journal-title":"Phytother. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"325","DOI":"10.5487\/TR.2017.33.4.325","article-title":"Anti-Inflammatory Effect of 3-Bromo-4,5-Dihydroxybenzaldehyde, a Component of Polysiphonia morrowii, In Vivo and In Vitro","volume":"33","author":"Kang","year":"2017","journal-title":"Toxicol. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1007\/s12275-011-1035-z","article-title":"In Vitro antiviral activity of red alga, Polysiphonia morrowii extract and its bromophenols against fish pathogenic infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus","volume":"49","author":"Kim","year":"2011","journal-title":"J. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1016\/j.biopha.2018.04.121","article-title":"Bis (3-bromo-4,5-dihydroxybenzyl) ether, a novel bromophenol from the marine red alga Polysiphonia morrowii that suppresses LPS-induced inflammatory response by inhibiting ROS-mediated ERK signaling pathway in RAW 264.7 macrophages","volume":"103","author":"Choi","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"ref_19","first-page":"45","article-title":"In vitro Anti-bacterial and Anti-scuticociliate Activities of Extract and Bromophenols of the Marine Red Alga Polysiphonia morrowii with Structure-activity Relationships","volume":"47","author":"Kang","year":"2014","journal-title":"Korean J. Fish. Aquat. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"11638","DOI":"10.1038\/s41598-018-30086-5","article-title":"Biological and epidemiological evidence of anti-allergic effects of traditional Japanese food ume (Prunus mume)","volume":"8","author":"Kono","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1886","DOI":"10.4049\/jimmunol.1302520","article-title":"Does \u03b2-Hexosaminidase Function Only as a Degranulation Indicator in Mast Cells? The Primary Role of \u03b2-Hexosaminidase in Mast Cell Granules","volume":"193","author":"Fukuishi","year":"2014","journal-title":"J. Immunol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/35018500","article-title":"Structure of the Fc fragment of human IgE bound to its high-affinity receptor Fc\u03b5RI\u03b1","volume":"406","author":"Garman","year":"2000","journal-title":"Nature"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.aca.2004.03.031","article-title":"Combined microwave-assisted isolation and solid-phase purification procedures prior to the chromatographic determination of phenolic compounds in plant materials","volume":"513","year":"2004","journal-title":"Anal. Chim. Acta"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Mlcek, J., Jurikova, T., Skrovankova, S., and Sochor, J. (2016). Quercetin and Its Anti-Allergic Immune Response. Molecules, 21.","DOI":"10.3390\/molecules21050623"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1177\/1721727X1301100202","article-title":"Pharmacological importance of simple phenolic compounds on inflammation, cell proliferation and apoptosis with a special reference to \u03b2-D-salicin and hydroxybenzoic acid","volume":"11","author":"Mahdi","year":"2013","journal-title":"Eur. J. Inflamm."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.taap.2017.02.002","article-title":"Anti-allergic activity of 2,4,6-trihydroxy-3-geranylacetophenone (tHGA) via attenuation of IgE-mediated mast cell activation and inhibition of passive systemic anaphylaxis","volume":"319","author":"Tan","year":"2017","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.antiviral.2006.12.005","article-title":"Protocatechuic aldehyde inhibits hepatitis B virus replication both in vitro and in vivo","volume":"74","author":"Zhou","year":"2007","journal-title":"Antivir. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.3389\/fphar.2018.01441","article-title":"Anti-allergic Inflammatory Effects of the Essential Oil From Fruits of Zanthoxylum coreanum Nakai","volume":"9","author":"Guo","year":"2018","journal-title":"Front. Pharmacol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.procbio.2011.12.014","article-title":"Marine algae as a potential pharmaceutical source for anti-allergic therapeutics","volume":"47","author":"Vo","year":"2012","journal-title":"Process Biochem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1067\/mai.2000.106485","article-title":"The diverse potential effector and immunoregulatory roles of mast cells in allergic disease","volume":"105","author":"Williams","year":"2000","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.4049\/jimmunol.174.2.1063","article-title":"Mast Cells, Basophils, and Eosinophils Acquire Constitutive IL-4 and IL-13 Transcripts during Lineage Differentiation That Are Sufficient for Rapid Cytokine Production","volume":"174","author":"Gessner","year":"2005","journal-title":"J. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.cyto.2015.05.019","article-title":"Mast cell production and response to IL-4 and IL-13","volume":"75","author":"McLeod","year":"2015","journal-title":"Cytokine"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1258\/0007142001903625","article-title":"Th-2 cytokines in allergic disease","volume":"56","author":"Robinson","year":"2000","journal-title":"Br. Med. Bull."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/1465-9921-11-28","article-title":"Elevation of IL-6 in the allergic asthmatic airway is independent of inflammation but associates with loss of central airway function","volume":"11","author":"Allard","year":"2010","journal-title":"Respir. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1080\/09540105.2018.1436523","article-title":"Anti-allergic and anti-atopic dermatitis effects of Gardenia Fructus extract","volume":"29","author":"Debnath","year":"2018","journal-title":"Food Agric. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1476-7961-9-4","article-title":"Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L.","volume":"9","author":"Sohn","year":"2011","journal-title":"Clin. Mol. Allergy"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"273","DOI":"10.22146\/ijc.21342","article-title":"3,4-dihydroxybenzoic acid and 3,4-dihydroxybenzaldehyde from the fern Trichomanes Chinense L.; Isolation, antimicrobial and antioxidant Properties","volume":"12","author":"Syafni","year":"2012","journal-title":"Indones. J. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.jaci.2010.10.043","article-title":"Peroxisome proliferator\u2013activated receptor \u03b3\u2013mediated suppression of dendritic cell function prevents the onset of atopic dermatitis in NC\/Tnd mice","volume":"127","author":"Jung","year":"2011","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.1016\/j.jaci.2012.10.012","article-title":"DJ-1 regulates mast cell activation and IgE-mediated allergic responses","volume":"131","author":"Kim","year":"2012","journal-title":"J. Allergy Clin. Immunol."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/20\/2\/133\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:17:40Z","timestamp":1760134660000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/20\/2\/133"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,10]]},"references-count":39,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["md20020133"],"URL":"https:\/\/doi.org\/10.3390\/md20020133","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,10]]}}}