{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:48:22Z","timestamp":1790758102376,"version":"4.1.0"},"reference-count":57,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T00:00:00Z","timestamp":1653609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2020R1A2C2012608"],"award-info":[{"award-number":["NRF-2020R1A2C2012608"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Advanced glycation end-products (AGEs) play a vital role in the pathogenesis of diabetic complications. Methylglyoxal (MGO), one of the major precursors of AGEs, is a highly reactive dicarbonyl compound that plays an important role in the pathogenesis of diabetic nephropathy. This study was designed to evaluate the therapeutic potential of phlorotannin-rich Ecklonia cava extract (ECE) on MGO-induced diabetic nephropathy in in vitro models using mouse glomerular mesangial cells. ECE showed anti-glycation activity via breaking of AGEs-collagen cross-links and inhibition of AGEs formation and AGE-collagen cross-linking formation. The renoprotective effects were determined by assessing intracellular reactive oxygen species (ROS) and MGO accumulation, cell apoptosis, and the Nrf-2\/ARE signaling pathway. MGO-induced renal damage, intracellular ROS production level, and MGO-protein adduct accumulation were significantly decreased by pretreating ECE. Moreover, ECE pretreatment exhibited preventive properties against MGO-induced dicarbonyl stress via activation of the Nrf2\/ARE signaling pathway and reduction of RAGE protein expression in mouse glomerular mesangial cells. Collectively, these results indicated potential anti-glycation properties and prominent preventive effects of ECE against MGO-induced renal damage. Additionally, ECE may be utilized for the management of AGE-related diabetic nephropathy.<\/jats:p>","DOI":"10.3390\/md20060355","type":"journal-article","created":{"date-parts":[[2022,5,28]],"date-time":"2022-05-28T01:40:45Z","timestamp":1653702045000},"page":"355","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Therapeutic Potential of Phlorotannin-Rich Ecklonia cava Extract on Methylglyoxal-Induced Diabetic Nephropathy in In Vitro Model"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5438-3209","authenticated-orcid":false,"given":"Chi-Heung","family":"Cho","sequence":"first","affiliation":[{"name":"Division of Functional Food Research, Korea Food Research Institute, Wanju 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3863-5680","authenticated-orcid":false,"given":"Chang-Jun","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Functional Food Research, Korea Food Research Institute, Wanju 55365, Korea"},{"name":"Department of Food Science and Technology, Chonbuk National University, Jeonju 54896, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Gyeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Functional Food Research, Korea Food Research Institute, Wanju 55365, Korea"},{"name":"Department of Food Biotechnology, University of Science and Technology, Daejeon 34113, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5797-500X","authenticated-orcid":false,"given":"Bomi","family":"Ryu","sequence":"additional","affiliation":[{"name":"Marine Science Institute, Jeju National University, Jeju 63333, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Geon","family":"Je","sequence":"additional","affiliation":[{"name":"Marine Science Institute, Jeju National University, Jeju 63333, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoonsook","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Functional Food Research, Korea Food Research Institute, Wanju 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2920-4213","authenticated-orcid":false,"given":"Sang-Hoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Functional Food Research, Korea Food Research Institute, Wanju 55365, Korea"},{"name":"Department of Food Biotechnology, University of Science and Technology, Daejeon 34113, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.semnephrol.2007.01.006","article-title":"AGE, RAGE, and ROS in diabetic nephropathy","volume":"27","author":"Tan","year":"2007","journal-title":"Semin. 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