{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:51:47Z","timestamp":1790877107188,"version":"4.1.0"},"reference-count":79,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T00:00:00Z","timestamp":1657843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FONDECYT","award":["3210662"],"award-info":[{"award-number":["3210662"]}]},{"name":"FONDECYT","award":["AFB170007"],"award-info":[{"award-number":["AFB170007"]}]},{"name":"FONDECYT","award":["ID19I10412"],"award-info":[{"award-number":["ID19I10412"]}]},{"name":"FONDECYT","award":["1210107"],"award-info":[{"award-number":["1210107"]}]},{"name":"FONDECYT","award":["1221609"],"award-info":[{"award-number":["1221609"]}]},{"name":"FONDECYT","award":["FB210015"],"award-info":[{"award-number":["FB210015"]}]},{"name":"CONICYT PIA\/APOYO CCTE","award":["3210662"],"award-info":[{"award-number":["3210662"]}]},{"name":"CONICYT PIA\/APOYO CCTE","award":["AFB170007"],"award-info":[{"award-number":["AFB170007"]}]},{"name":"CONICYT PIA\/APOYO CCTE","award":["ID19I10412"],"award-info":[{"award-number":["ID19I10412"]}]},{"name":"CONICYT PIA\/APOYO CCTE","award":["1210107"],"award-info":[{"award-number":["1210107"]}]},{"name":"CONICYT PIA\/APOYO CCTE","award":["1221609"],"award-info":[{"award-number":["1221609"]}]},{"name":"CONICYT PIA\/APOYO CCTE","award":["FB210015"],"award-info":[{"award-number":["FB210015"]}]},{"name":"Fondef","award":["3210662"],"award-info":[{"award-number":["3210662"]}]},{"name":"Fondef","award":["AFB170007"],"award-info":[{"award-number":["AFB170007"]}]},{"name":"Fondef","award":["ID19I10412"],"award-info":[{"award-number":["ID19I10412"]}]},{"name":"Fondef","award":["1210107"],"award-info":[{"award-number":["1210107"]}]},{"name":"Fondef","award":["1221609"],"award-info":[{"award-number":["1221609"]}]},{"name":"Fondef","award":["FB210015"],"award-info":[{"award-number":["FB210015"]}]},{"name":"Fondecyt Regular","award":["3210662"],"award-info":[{"award-number":["3210662"]}]},{"name":"Fondecyt Regular","award":["AFB170007"],"award-info":[{"award-number":["AFB170007"]}]},{"name":"Fondecyt Regular","award":["ID19I10412"],"award-info":[{"award-number":["ID19I10412"]}]},{"name":"Fondecyt Regular","award":["1210107"],"award-info":[{"award-number":["1210107"]}]},{"name":"Fondecyt Regular","award":["1221609"],"award-info":[{"award-number":["1221609"]}]},{"name":"Fondecyt Regular","award":["FB210015"],"award-info":[{"award-number":["FB210015"]}]},{"name":"ANID BASAL (CENAMAD)","award":["3210662"],"award-info":[{"award-number":["3210662"]}]},{"name":"ANID BASAL (CENAMAD)","award":["AFB170007"],"award-info":[{"award-number":["AFB170007"]}]},{"name":"ANID BASAL (CENAMAD)","award":["ID19I10412"],"award-info":[{"award-number":["ID19I10412"]}]},{"name":"ANID BASAL (CENAMAD)","award":["1210107"],"award-info":[{"award-number":["1210107"]}]},{"name":"ANID BASAL (CENAMAD)","award":["1221609"],"award-info":[{"award-number":["1221609"]}]},{"name":"ANID BASAL (CENAMAD)","award":["FB210015"],"award-info":[{"award-number":["FB210015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Codium bernabei is a green alga that grows on Chilean coasts. The composition of its structural polysaccharides is still unknown. Hence, the aim of this work is to isolate and characterize the hot water extracted polysaccharide fractions. For this purpose, the water extracts were further precipitated in alcohol (TPs) and acid media (APs), respectively. Both fractions were characterized using different physicochemical techniques such as GC-MS, GPC, FTIR, TGA, and SEM. It is confirmed that the extracted fractions are mainly made of sulfated galactan unit, with a degree of sulfation of 19.3% (TPs) and 17.4% (ATs) and a protein content of 3.5% in APs and 15.6% in TPs. Other neutral sugars such as xylose, glucose, galactose, fucose, mannose, and arabinose were found in a molar ratio (0.05:0.6:1.0:0.02:0.14:0.11) for TPs and (0.05:0.31:1.0:0.03:0.1:0.13) for ATs. The molecular weight of the polysaccharide samples was lower than 20 kDa. Both polysaccharides were thermally stable (Tonset &gt; 190 \u00b0C) and showed antioxidant activity according to the ABTS\u2022+ and DPPH tests, where TPs fractions had higher scavenging activity (35%) compared to the APs fractions. The PT and APTTS assays were used to measure the anticoagulant activity of the polysaccharide fractions. In general, the PT activity of the TPs and APs was not different from normal plasma values. The exception was the TPs treatment at 1000 \u00b5g mL\u22121 concentration. The APTTS test revealed that clotting time for both polysaccharides was prolonged regarding normal values at 1000 \u00b5g mL\u22121. Finally, the antitumor test in colorectal carcinoma (HTC-116) cell line, breast cancer (MCF-7) and human leukemia (HL-60) cell lines showed the cytotoxic effect of TPs and APs. Those results suggest the potential biotechnological application of sulfate galactan polysaccharides isolated from a Chilean marine resource.<\/jats:p>","DOI":"10.3390\/md20070458","type":"journal-article","created":{"date-parts":[[2022,7,17]],"date-time":"2022-07-17T22:57:06Z","timestamp":1658098626000},"page":"458","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9383-8045","authenticated-orcid":false,"given":"Fabian A.","family":"Figueroa","sequence":"first","affiliation":[{"name":"Laboratorio de Qu\u00edmica de Productos Naturales, Departamento de Bot\u00e1nica, Facultad de Ciencias Naturales y Oceanogr\u00e1ficas, Universidad de Concepci\u00f3n, Casilla 160-C, Concepci\u00f3n 4030000, Chile"},{"name":"Unidad de Desarrollo Tecnol\u00f3gico (UDT), Universidad de Concepci\u00f3n, Avda. Cordillera No. 2634, Parque Industrial Coronel, Coronel 4191996, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto T.","family":"Abdala-D\u00edaz","sequence":"additional","affiliation":[{"name":"Departamento de Ecolog\u00eda, Facultad de Ciencias, Instituto de Biotecnolog\u00eda y Desarrollo Azul (IBYDA), Universidad de M\u00e1laga, Campus de Teatinos s\/n, 29071 M\u00e1laga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8562-3842","authenticated-orcid":false,"given":"Claudia","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Laboratorio de Qu\u00edmica de Productos Naturales, Departamento de Bot\u00e1nica, Facultad de Ciencias Naturales y Oceanogr\u00e1ficas, Universidad de Concepci\u00f3n, Casilla 160-C, Concepci\u00f3n 4030000, Chile"},{"name":"Unidad de Desarrollo Tecnol\u00f3gico (UDT), Universidad de Concepci\u00f3n, Avda. Cordillera No. 2634, Parque Industrial Coronel, Coronel 4191996, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2170-6323","authenticated-orcid":false,"given":"Virginia","family":"Casas-Arrojo","sequence":"additional","affiliation":[{"name":"Departamento de Ecolog\u00eda, Facultad de Ciencias, Instituto de Biotecnolog\u00eda y Desarrollo Azul (IBYDA), Universidad de M\u00e1laga, Campus de Teatinos s\/n, 29071 M\u00e1laga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Nesic","sequence":"additional","affiliation":[{"name":"Laboratorio de Qu\u00edmica de Productos Naturales, Departamento de Bot\u00e1nica, Facultad de Ciencias Naturales y Oceanogr\u00e1ficas, Universidad de Concepci\u00f3n, Casilla 160-C, Concepci\u00f3n 4030000, Chile"},{"name":"Vinca Institute of Nuclear Sciences\u2014National Institute of the Republic of Serbia, University of Belgrade, 12\u201314 Mike Petrovi\u0107a Street, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Tapia","sequence":"additional","affiliation":[{"name":"Laboratorio de Especialidad Cl\u00ednica D\u00e1vila-OMESA, Recoleta 464, Recoleta, Santiago 8431657, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Laboratorio de Especialidad Cl\u00ednica D\u00e1vila-OMESA, Recoleta 464, Recoleta, Santiago 8431657, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1477-2549","authenticated-orcid":false,"given":"Oscar","family":"Valdes","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n de Estudios Avanzados del Maule (CIEAM), Vicerrector\u00eda de Investigaci\u00f3n y Postgrado, Universidad Cat\u00f3lica del Maule, Talca 3480005, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2710-462X","authenticated-orcid":false,"given":"Carolina","family":"Parra","sequence":"additional","affiliation":[{"name":"Laboratorio de Recursos Renovables, Centro de Biotecnolog\u00eda, Barrio Universitario s\/n, Universidad de Concepci\u00f3n, Concepci\u00f3n 4030000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5765-8300","authenticated-orcid":false,"given":"Gast\u00f3n","family":"Bravo-Arrepol","sequence":"additional","affiliation":[{"name":"Unidad de Desarrollo Tecnol\u00f3gico (UDT), Universidad de Concepci\u00f3n, Avda. Cordillera No. 2634, Parque Industrial Coronel, Coronel 4191996, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Soto","sequence":"additional","affiliation":[{"name":"Laboratorio de Qu\u00edmica de Productos Naturales, Departamento de Bot\u00e1nica, Facultad de Ciencias Naturales y Oceanogr\u00e1ficas, Universidad de Concepci\u00f3n, Casilla 160-C, Concepci\u00f3n 4030000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Becerra","sequence":"additional","affiliation":[{"name":"Laboratorio de Qu\u00edmica de Productos Naturales, Departamento de Bot\u00e1nica, Facultad de Ciencias Naturales y Oceanogr\u00e1ficas, Universidad de Concepci\u00f3n, Casilla 160-C, Concepci\u00f3n 4030000, Chile"},{"name":"Unidad de Desarrollo Tecnol\u00f3gico (UDT), Universidad de Concepci\u00f3n, Avda. 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Collao 1202, Concepci\u00f3n 4051381, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Stiger-Pouvreau, V., Bourgougnon, N., and Deslandes, E. (2016). Carbohydrates from Seaweeds. Seaweed in Health and Disease Prevention, Elsevier Inc.","DOI":"10.1016\/B978-0-12-802772-1.00008-7"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Gomez-Zavaglia, A., Prieto Lage, M.A., Jimenez-Lopez, C., Mejuto, J.C., and Simal-Gandara, J. (2019). The potential of seaweeds as a source of functional ingredients of prebiotic and antioxidant value. Antioxidants, 8.","DOI":"10.3390\/antiox8090406"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Salehi, B., Sharifi-Rad, J., Seca, A.M.L., Pinto, D.C.G.A., Michalak, I., Trincone, A., Mishra, A.P., Nigam, M., Zam, W., and Martins, N. (2019). 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