{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T19:16:17Z","timestamp":1788981377015,"version":"build-2803163510"},"reference-count":62,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,16]],"date-time":"2022-07-16T00:00:00Z","timestamp":1657929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Zhejiang Major Program of Science and Technology","award":["2021C02069-7"],"award-info":[{"award-number":["2021C02069-7"]}]},{"name":"Zhejiang Major Program of Science and Technology","award":["LZ20C190001"],"award-info":[{"award-number":["LZ20C190001"]}]},{"name":"Zhejiang Major Program of Science and Technology","award":["LQ21C190004"],"award-info":[{"award-number":["LQ21C190004"]}]},{"name":"Zhejiang Major Program of Science and Technology","award":["32073002"],"award-info":[{"award-number":["32073002"]}]},{"name":"Zhejiang Major Program of Science and Technology","award":["31902404"],"award-info":[{"award-number":["31902404"]}]},{"name":"Zhejiang Major Program of Science and Technology","award":["32002359"],"award-info":[{"award-number":["32002359"]}]},{"name":"Zhejiang Major Program of Science and Technology","award":["2021S014"],"award-info":[{"award-number":["2021S014"]}]},{"name":"Zhejiang Major Program of Science and Technology","award":["KF2022005"],"award-info":[{"award-number":["KF2022005"]}]},{"name":"Zhejiang Major Program of Science and Technology","award":["NBHY-2017-S4"],"award-info":[{"award-number":["NBHY-2017-S4"]}]},{"name":"Zhejiang Major Program of Science and Technology","award":["2020A1515011533"],"award-info":[{"award-number":["2020A1515011533"]}]},{"name":"Zhejiang Major Program of Science and Technology","award":["CARS-49"],"award-info":[{"award-number":["CARS-49"]}]},{"name":"Key Natural Science Foundation of Zhejiang","award":["2021C02069-7"],"award-info":[{"award-number":["2021C02069-7"]}]},{"name":"Key Natural Science Foundation of Zhejiang","award":["LZ20C190001"],"award-info":[{"award-number":["LZ20C190001"]}]},{"name":"Key Natural Science Foundation of Zhejiang","award":["LQ21C190004"],"award-info":[{"award-number":["LQ21C190004"]}]},{"name":"Key Natural Science Foundation of Zhejiang","award":["32073002"],"award-info":[{"award-number":["32073002"]}]},{"name":"Key Natural Science Foundation of Zhejiang","award":["31902404"],"award-info":[{"award-number":["31902404"]}]},{"name":"Key Natural Science Foundation of Zhejiang","award":["32002359"],"award-info":[{"award-number":["32002359"]}]},{"name":"Key Natural Science Foundation of Zhejiang","award":["2021S014"],"award-info":[{"award-number":["2021S014"]}]},{"name":"Key Natural Science Foundation of Zhejiang","award":["KF2022005"],"award-info":[{"award-number":["KF2022005"]}]},{"name":"Key Natural Science Foundation of Zhejiang","award":["NBHY-2017-S4"],"award-info":[{"award-number":["NBHY-2017-S4"]}]},{"name":"Key Natural Science Foundation of Zhejiang","award":["2020A1515011533"],"award-info":[{"award-number":["2020A1515011533"]}]},{"name":"Key Natural Science Foundation of Zhejiang","award":["CARS-49"],"award-info":[{"award-number":["CARS-49"]}]},{"name":"Natural Science Foundation of Zhejiang","award":["2021C02069-7"],"award-info":[{"award-number":["2021C02069-7"]}]},{"name":"Natural Science Foundation of Zhejiang","award":["LZ20C190001"],"award-info":[{"award-number":["LZ20C190001"]}]},{"name":"Natural Science Foundation of Zhejiang","award":["LQ21C190004"],"award-info":[{"award-number":["LQ21C190004"]}]},{"name":"Natural Science Foundation of Zhejiang","award":["32073002"],"award-info":[{"award-number":["32073002"]}]},{"name":"Natural Science Foundation of Zhejiang","award":["31902404"],"award-info":[{"award-number":["31902404"]}]},{"name":"Natural Science Foundation of Zhejiang","award":["32002359"],"award-info":[{"award-number":["32002359"]}]},{"name":"Natural Science Foundation of Zhejiang","award":["2021S014"],"award-info":[{"award-number":["2021S014"]}]},{"name":"Natural Science Foundation of Zhejiang","award":["KF2022005"],"award-info":[{"award-number":["KF2022005"]}]},{"name":"Natural Science Foundation of Zhejiang","award":["NBHY-2017-S4"],"award-info":[{"award-number":["NBHY-2017-S4"]}]},{"name":"Natural Science Foundation of Zhejiang","award":["2020A1515011533"],"award-info":[{"award-number":["2020A1515011533"]}]},{"name":"Natural Science Foundation of Zhejiang","award":["CARS-49"],"award-info":[{"award-number":["CARS-49"]}]},{"name":"National Science Foundation of China","award":["2021C02069-7"],"award-info":[{"award-number":["2021C02069-7"]}]},{"name":"National Science Foundation of China","award":["LZ20C190001"],"award-info":[{"award-number":["LZ20C190001"]}]},{"name":"National Science Foundation of China","award":["LQ21C190004"],"award-info":[{"award-number":["LQ21C190004"]}]},{"name":"National Science Foundation of China","award":["32073002"],"award-info":[{"award-number":["32073002"]}]},{"name":"National Science Foundation of China","award":["31902404"],"award-info":[{"award-number":["31902404"]}]},{"name":"National Science Foundation of China","award":["32002359"],"award-info":[{"award-number":["32002359"]}]},{"name":"National Science Foundation of China","award":["2021S014"],"award-info":[{"award-number":["2021S014"]}]},{"name":"National Science Foundation of China","award":["KF2022005"],"award-info":[{"award-number":["KF2022005"]}]},{"name":"National Science Foundation of China","award":["NBHY-2017-S4"],"award-info":[{"award-number":["NBHY-2017-S4"]}]},{"name":"National Science Foundation of China","award":["2020A1515011533"],"award-info":[{"award-number":["2020A1515011533"]}]},{"name":"National Science Foundation of China","award":["CARS-49"],"award-info":[{"award-number":["CARS-49"]}]},{"name":"Ningbo Public Benefit Research Key Project","award":["2021C02069-7"],"award-info":[{"award-number":["2021C02069-7"]}]},{"name":"Ningbo Public Benefit Research Key Project","award":["LZ20C190001"],"award-info":[{"award-number":["LZ20C190001"]}]},{"name":"Ningbo Public Benefit Research Key Project","award":["LQ21C190004"],"award-info":[{"award-number":["LQ21C190004"]}]},{"name":"Ningbo Public Benefit Research Key Project","award":["32073002"],"award-info":[{"award-number":["32073002"]}]},{"name":"Ningbo Public Benefit Research Key Project","award":["31902404"],"award-info":[{"award-number":["31902404"]}]},{"name":"Ningbo Public Benefit Research Key Project","award":["32002359"],"award-info":[{"award-number":["32002359"]}]},{"name":"Ningbo Public Benefit Research Key Project","award":["2021S014"],"award-info":[{"award-number":["2021S014"]}]},{"name":"Ningbo Public Benefit Research Key Project","award":["KF2022005"],"award-info":[{"award-number":["KF2022005"]}]},{"name":"Ningbo Public Benefit Research Key Project","award":["NBHY-2017-S4"],"award-info":[{"award-number":["NBHY-2017-S4"]}]},{"name":"Ningbo Public Benefit Research Key Project","award":["2020A1515011533"],"award-info":[{"award-number":["2020A1515011533"]}]},{"name":"Ningbo Public Benefit Research Key Project","award":["CARS-49"],"award-info":[{"award-number":["CARS-49"]}]},{"name":"Zhejiang Provincial Top Key Discipline","award":["2021C02069-7"],"award-info":[{"award-number":["2021C02069-7"]}]},{"name":"Zhejiang Provincial Top Key Discipline","award":["LZ20C190001"],"award-info":[{"award-number":["LZ20C190001"]}]},{"name":"Zhejiang Provincial Top Key Discipline","award":["LQ21C190004"],"award-info":[{"award-number":["LQ21C190004"]}]},{"name":"Zhejiang Provincial Top Key Discipline","award":["32073002"],"award-info":[{"award-number":["32073002"]}]},{"name":"Zhejiang Provincial Top Key Discipline","award":["31902404"],"award-info":[{"award-number":["31902404"]}]},{"name":"Zhejiang Provincial Top Key Discipline","award":["32002359"],"award-info":[{"award-number":["32002359"]}]},{"name":"Zhejiang Provincial Top Key Discipline","award":["2021S014"],"award-info":[{"award-number":["2021S014"]}]},{"name":"Zhejiang Provincial Top Key Discipline","award":["KF2022005"],"award-info":[{"award-number":["KF2022005"]}]},{"name":"Zhejiang Provincial Top Key Discipline","award":["NBHY-2017-S4"],"award-info":[{"award-number":["NBHY-2017-S4"]}]},{"name":"Zhejiang Provincial Top Key Discipline","award":["2020A1515011533"],"award-info":[{"award-number":["2020A1515011533"]}]},{"name":"Zhejiang Provincial Top Key Discipline","award":["CARS-49"],"award-info":[{"award-number":["CARS-49"]}]},{"name":"Demonstration Project for Innovative Development of Marine Economy","award":["2021C02069-7"],"award-info":[{"award-number":["2021C02069-7"]}]},{"name":"Demonstration Project for Innovative Development of Marine Economy","award":["LZ20C190001"],"award-info":[{"award-number":["LZ20C190001"]}]},{"name":"Demonstration Project for Innovative Development of Marine Economy","award":["LQ21C190004"],"award-info":[{"award-number":["LQ21C190004"]}]},{"name":"Demonstration Project for Innovative Development of Marine Economy","award":["32073002"],"award-info":[{"award-number":["32073002"]}]},{"name":"Demonstration Project for Innovative Development of Marine Economy","award":["31902404"],"award-info":[{"award-number":["31902404"]}]},{"name":"Demonstration Project for Innovative Development of Marine Economy","award":["32002359"],"award-info":[{"award-number":["32002359"]}]},{"name":"Demonstration Project for Innovative Development of Marine Economy","award":["2021S014"],"award-info":[{"award-number":["2021S014"]}]},{"name":"Demonstration Project for Innovative Development of Marine Economy","award":["KF2022005"],"award-info":[{"award-number":["KF2022005"]}]},{"name":"Demonstration Project for Innovative Development of Marine Economy","award":["NBHY-2017-S4"],"award-info":[{"award-number":["NBHY-2017-S4"]}]},{"name":"Demonstration Project for Innovative Development of Marine Economy","award":["2020A1515011533"],"award-info":[{"award-number":["2020A1515011533"]}]},{"name":"Demonstration Project for Innovative Development of Marine Economy","award":["CARS-49"],"award-info":[{"award-number":["CARS-49"]}]},{"name":"Natural Science Foundation of Guangdong Province","award":["2021C02069-7"],"award-info":[{"award-number":["2021C02069-7"]}]},{"name":"Natural Science Foundation of Guangdong Province","award":["LZ20C190001"],"award-info":[{"award-number":["LZ20C190001"]}]},{"name":"Natural Science Foundation of Guangdong Province","award":["LQ21C190004"],"award-info":[{"award-number":["LQ21C190004"]}]},{"name":"Natural Science Foundation of Guangdong Province","award":["32073002"],"award-info":[{"award-number":["32073002"]}]},{"name":"Natural Science Foundation of Guangdong Province","award":["31902404"],"award-info":[{"award-number":["31902404"]}]},{"name":"Natural Science Foundation of Guangdong Province","award":["32002359"],"award-info":[{"award-number":["32002359"]}]},{"name":"Natural Science Foundation of Guangdong Province","award":["2021S014"],"award-info":[{"award-number":["2021S014"]}]},{"name":"Natural Science Foundation of Guangdong Province","award":["KF2022005"],"award-info":[{"award-number":["KF2022005"]}]},{"name":"Natural Science Foundation of Guangdong Province","award":["NBHY-2017-S4"],"award-info":[{"award-number":["NBHY-2017-S4"]}]},{"name":"Natural Science Foundation of Guangdong Province","award":["2020A1515011533"],"award-info":[{"award-number":["2020A1515011533"]}]},{"name":"Natural Science Foundation of Guangdong Province","award":["CARS-49"],"award-info":[{"award-number":["CARS-49"]}]},{"name":"China Agricultural Research System","award":["2021C02069-7"],"award-info":[{"award-number":["2021C02069-7"]}]},{"name":"China Agricultural Research System","award":["LZ20C190001"],"award-info":[{"award-number":["LZ20C190001"]}]},{"name":"China Agricultural Research System","award":["LQ21C190004"],"award-info":[{"award-number":["LQ21C190004"]}]},{"name":"China Agricultural Research System","award":["32073002"],"award-info":[{"award-number":["32073002"]}]},{"name":"China Agricultural Research System","award":["31902404"],"award-info":[{"award-number":["31902404"]}]},{"name":"China Agricultural Research System","award":["32002359"],"award-info":[{"award-number":["32002359"]}]},{"name":"China Agricultural Research System","award":["2021S014"],"award-info":[{"award-number":["2021S014"]}]},{"name":"China Agricultural Research System","award":["KF2022005"],"award-info":[{"award-number":["KF2022005"]}]},{"name":"China Agricultural Research System","award":["NBHY-2017-S4"],"award-info":[{"award-number":["NBHY-2017-S4"]}]},{"name":"China Agricultural Research System","award":["2020A1515011533"],"award-info":[{"award-number":["2020A1515011533"]}]},{"name":"China Agricultural Research System","award":["CARS-49"],"award-info":[{"award-number":["CARS-49"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Hemocyanins present in the hemolymph of invertebrates are multifunctional proteins that are responsible for oxygen transport and play crucial roles in the immune system. They have also been identified as a source of antimicrobial peptides during infection in mollusks. Hemocyanin has also been identified in the cephalopod ancestor Nautilus, but antimicrobial peptides derived from the hemocyanin of Nautilus pompilius have not been reported. Here, the bactericidal activity of six predicted peptides from N. pompilius hemocyanin and seven mutant peptides was analyzed. Among those peptides, a mutant peptide with 15 amino acids (1RVFAGFLRHGIKRSR15), NpHM4, showed relatively high antibacterial activity. NpHM4 was determined to have typical antimicrobial peptide characteristics, including a positive charge (+5.25) and a high hydrophobic residue ratio (40%), and it was predicted to form an alpha-helical structure. In addition, NpHM4 exhibited significant antibacterial activity against Gram-negative bacteria (MBC = 30 \u03bcM for Vibrio alginolyticus), with no cytotoxicity to mammalian cells even at a high concentration of 180 \u00b5M. Upon contact with V. alginolyticus cells, we confirmed that the bactericidal activity of NpHM4 was coupled with membrane permeabilization, which was further confirmed via ultrastructural images using a scanning electron microscope. Therefore, our study provides a rationalization for the development and optimization of antimicrobial peptide from the cephalopod ancestor Nautilus, paving the way for future novel AMP development with broad applications.<\/jats:p>","DOI":"10.3390\/md20070459","type":"journal-article","created":{"date-parts":[[2022,7,17]],"date-time":"2022-07-17T22:57:06Z","timestamp":1658098626000},"page":"459","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Functional Characterization, Antimicrobial Effects, and Potential Antibacterial Mechanisms of NpHM4, a Derived Peptide of Nautilus pompilius Hemocyanin"],"prefix":"10.3390","volume":"20","author":[{"given":"Chun","family":"Yuan","sequence":"first","affiliation":[{"name":"Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China"},{"name":"Ninghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ningbo 315604, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Zheng","sequence":"additional","affiliation":[{"name":"Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China"},{"name":"School of Marine Science, Ningbo University, Ningbo 315211, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunna","family":"Liu","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology and Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou 510301, China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 510301, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbin","family":"Yuan","sequence":"additional","affiliation":[{"name":"Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China"},{"name":"School of Marine Science, Ningbo University, Ningbo 315211, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0789-4938","authenticated-orcid":false,"given":"Yang","family":"Zhang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology and Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou 510301, China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 510301, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Mao","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology and Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou 510301, China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 510301, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongbo","family":"Bao","sequence":"additional","affiliation":[{"name":"Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China"},{"name":"Ninghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ningbo 315604, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.coph.2014.09.006","article-title":"Update on the antibiotic resistance crisis","volume":"18","author":"Rossolini","year":"2014","journal-title":"Curr. Opin. Pharmacol."},{"key":"ref_2","unstructured":"World Health Organization (2021, July 19). Antimicrobial Resistance: Global Report on Surveillance. Available online: https:\/\/www.who.int\/publications\/i\/item\/9789241564748."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"R14","DOI":"10.1016\/j.cub.2015.11.017","article-title":"Antimicrobial peptides","volume":"26","author":"Zhang","year":"2016","journal-title":"Curr. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/s13238-010-0004-3","article-title":"Alpha-helical cationic antimicrobial peptides: Relationships of structure and function","volume":"1","author":"Huang","year":"2010","journal-title":"Protein Cell"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"325","DOI":"10.3389\/fmicb.2018.00325","article-title":"Designing antibacterial peptides with enhanced killing kinetics","volume":"9","author":"Waghu","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Murugan, R.N., Jacob, B., Ahn, M., Hwang, E., Sohn, H., Park, H.N., Lee, E., Seo, J.H., Cheong, C., and Nam, K.Y. (2013). De novo design and synthesis of ultra-short peptidomimetic antibiotics having dual antimicrobial and anti-inflammatory activities. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0080025"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3389\/fnins.2017.00073","article-title":"Membrane active antimicrobial peptides: Translating mechanistic insights to design","volume":"11","author":"Li","year":"2017","journal-title":"Front. Neurosci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"104294","DOI":"10.1016\/j.jprot.2021.104294","article-title":"Hemolymph proteins: An overview across marine arthropods and molluscs","volume":"245","author":"Gianazza","year":"2021","journal-title":"J. Proteom."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2361","DOI":"10.1016\/j.carres.2010.08.005","article-title":"Glycan structures and antiviral effect of the structural subunit rvh2 of rapana hemocyanin","volume":"345","author":"Dolashka","year":"2010","journal-title":"Carbohydr. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.fsi.2018.02.010","article-title":"Antibacterial activity of hemocyanin from red swamp crayfish (Procambarus clarkii)","volume":"75","author":"Qin","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.dci.2014.01.021","article-title":"Diverse immune functions of hemocyanins","volume":"45","author":"Coates","year":"2014","journal-title":"Dev. Comp. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"107930","DOI":"10.1016\/j.exppara.2020.107930","article-title":"Antichagasic effect of hemocyanin derived from antimicrobial peptides of Penaeus monodon shrimp","volume":"215","author":"Monteiro","year":"2020","journal-title":"Exp. Parasitol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.dci.2014.11.008","article-title":"Identification of candidate antimicrobial peptides derived from abalone hemocyanin","volume":"49","author":"Zhuang","year":"2015","journal-title":"Dev. Comp. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/0022-2011(91)90142-D","article-title":"Agglutination of bacteria and erythrocytes by serum from six species of marine molluscs","volume":"57","author":"Fisher","year":"1991","journal-title":"J. Invertebr. Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1038\/445153a","article-title":"Care for the community","volume":"445","year":"2007","journal-title":"Nature"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.fsi.2015.05.005","article-title":"Understanding the cephalopod immune system based on functional and molecular evidence","volume":"46","author":"Gestal","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0959-8030(92)90054-2","article-title":"Host defense mechanisms of cephalopods","volume":"2","author":"Ford","year":"1992","journal-title":"Annu. Rev. Fish Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1007\/s00726-018-2633-4","article-title":"Design of antimicrobial peptides from a cuttlefish database","volume":"50","author":"Houyvet","year":"2018","journal-title":"Amino Acids"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Nikapitiya, C., Dananjaya, S.H.S., Chandrarathna, H.P.S.U., de Zoysa, M., and Whang, I. (2020). Octominin: A novel synthetic anticandidal peptide derived from defense protein of Octopus minor. Mar. Drugs, 18.","DOI":"10.3390\/md18010056"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1002\/bies.201100001","article-title":"Cephalopod origin and evolution: A congruent picture emerging from fossils, development and molecules: Extant cephalopods are younger than previously realised and were under major selection to become agile, shell-less predators","volume":"33","author":"Vinther","year":"2011","journal-title":"Bioessays"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4924","DOI":"10.1002\/ece3.2248","article-title":"A revisited phylogeography of Nautilus pompilius","volume":"6","author":"Vandepas","year":"2016","journal-title":"Ecol. Evol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1038\/s41559-021-01448-6","article-title":"The genome of Nautilus pompilius illuminates eye evolution and biomineralization","volume":"5","author":"Zhang","year":"2021","journal-title":"Nat. Ecol. Evol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.jmb.2007.09.036","article-title":"Nautilus pompilius hemocyanin: 9 \u00c5 cryo-em structure and molecular model reveal the subunit pathway and the interfaces between the 70 functional units","volume":"374","author":"Gatsogiannis","year":"2007","journal-title":"J. Mol. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"9117","DOI":"10.1038\/s41598-020-65688-5","article-title":"A novel, rationally designed, hybrid antimicrobial peptide, inspired by cathelicidin and aurein, exhibits membrane-active mechanisms against Pseudomonas aeruginosa","volume":"10","author":"Klubthawee","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.addr.2014.10.013","article-title":"Strategies employed in the design and optimization of synthetic antimicrobial peptide amphiphiles with enhanced therapeutic potentials","volume":"78","author":"Ong","year":"2014","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"86","DOI":"10.3389\/fchem.2017.00086","article-title":"The human antimicrobial peptides dermcidin and ll-37 show novel distinct pathways in membrane interactions","volume":"5","author":"Zeth","year":"2017","journal-title":"Front. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1016\/j.biochi.2010.02.023","article-title":"Structural determinants of host defense peptides for antimicrobial activity and target cell selectivity","volume":"92","author":"Takahashi","year":"2010","journal-title":"Biochimie"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.biochi.2017.12.007","article-title":"Membrane permeabilization design of antimicrobial peptides based on Chikungunya virus fusion domain scaffold and its antibacterial activity against gram-positive Streptococcus pneumoniae in respiratory infection","volume":"146","author":"Yang","year":"2018","journal-title":"Biochimie"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e01821-19","DOI":"10.1128\/mBio.01821-19","article-title":"The ancestral n-terminal domain of big defensins drives bacterially triggered assembly into antimicrobial nanonets","volume":"10","author":"Loth","year":"2019","journal-title":"mBio"},{"key":"ref_30","first-page":"277","article-title":"The antibiotic resistance crisis: Part 1: Causes and threats","volume":"40","author":"Ventola","year":"2015","journal-title":"Pharm. Ther."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"9761","DOI":"10.1038\/srep09761","article-title":"In vitro properties of designed antimicrobial peptides that exhibit potent antipneumococcal activity and produces synergism in combination with penicillin","volume":"5","author":"Le","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.tips.2019.04.012","article-title":"The vast structural diversity of antimicrobial peptides","volume":"40","author":"Koehbach","year":"2019","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"25889","DOI":"10.1074\/jbc.273.40.25889","article-title":"Tarantula hemocyanin shows phenoloxidase activity","volume":"273","author":"Decker","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.ejps.2013.11.013","article-title":"Formulation of abalone hemocyanin with high antiviral activity and stability","volume":"53","author":"Zanjani","year":"2014","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.imlet.2017.10.008","article-title":"Glycosylation of hemocyanin in Litopenaeus vannamei is an antibacterial response feature","volume":"192","author":"Zhang","year":"2017","journal-title":"Immunol. Lett."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.fsi.2016.08.002","article-title":"Identification and characterization of an 18.4 kda antimicrobial truncation from shrimp Litopenaeus vannamei hemocyanin upon vibrio parahaemolyticus infection","volume":"56","author":"Wen","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Liscano, Y., Salamanca, C.H., Vargas, L., Cantor, S., Laverde-Rojas, V., and O\u00f1ate-Garz\u00f3n, J. (2019). Increases in hydrophilicity and charge on the polar face of alyteserin 1c helix change its selectivity towards gram-positive bacteria. Antibiotics, 8.","DOI":"10.3390\/antibiotics8040238"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Manzo, G., Scorciapino, M.A., Wadhwani, P., B\u00fcrck, J., Montaldo, N.P., Pintus, M., Sanna, R., Casu, M., Giuliani, A., and Pirri, G. (2015). Enhanced amphiphilic profile of a short \u03b2-stranded peptide improves its antimicrobial activity. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0116379"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"101229","DOI":"10.1016\/j.nantod.2021.101229","article-title":"Design, optimization, and nanotechnology of antimicrobial peptides: From exploration to applications","volume":"39","author":"Tan","year":"2021","journal-title":"Nano Today"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"7738","DOI":"10.1074\/jbc.M111.303602","article-title":"Roles of hydrophobicity and charge distribution of cationic antimicrobial peptides in peptide-membrane interactions","volume":"287","author":"Yin","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4596","DOI":"10.1021\/jp0689060","article-title":"Infrared study of the effect of hydration on the amide i band and aggregation properties of helical peptides","volume":"111","author":"Mukherjee","year":"2007","journal-title":"J. Phys. Chem. B"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/j.colsurfb.2016.02.003","article-title":"Role of amphipathicity and hydrophobicity in the balance between hemolysis and peptide\u2013membrane interactions of three related antimicrobial peptides","volume":"141","author":"Hollmann","year":"2016","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"25","DOI":"10.2174\/1568026615666150703121700","article-title":"Antimicrobial peptide structure and mechanism of action: A focus on the role of membrane structure","volume":"16","author":"Lee","year":"2016","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"194","DOI":"10.3389\/fcimb.2016.00194","article-title":"Antimicrobial peptides: An emerging category of therapeutic agents","volume":"6","author":"Mahlapuu","year":"2016","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/nrd3591","article-title":"Designing antimicrobial peptides: Form follows function","volume":"11","author":"Fjell","year":"2012","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"735926","DOI":"10.1016\/j.aquaculture.2020.735926","article-title":"Pvhs9 is a novel in silico predicted antimicrobial peptide derived from hemocyanin of Penaeus vannamei","volume":"530","author":"Yang","year":"2021","journal-title":"Aquaculture"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.biomaterials.2014.10.041","article-title":"Insights into the membrane interaction mechanism and antibacterial properties of chensinin-1b","volume":"37","author":"Sun","year":"2015","journal-title":"Biomaterials"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"737807","DOI":"10.1016\/j.aquaculture.2021.737807","article-title":"Antimicrobial activity of pvh4a, a peptide derived from histone h4 of Penaeus vannamei","volume":"549","author":"Yang","year":"2022","journal-title":"Aquaculture"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1007\/s00128-012-0695-7","article-title":"Biochemical effects of veterinary antibiotics on proliferation and cell cycle arrest of human hek293 cells","volume":"89","author":"Kim","year":"2012","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Gu, Y., Chen, X., Shang, C., Singh, K., Barzegar, M., Mahdavian, E., Salvatore, B.A., Jiang, S., and Huang, S. (2014). Fusarochromanone induces g1 cell cycle arrest and apoptosis in cos7 and hek293 cells. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0112641"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1007\/s00726-018-2575-x","article-title":"Prediction and characterization of a novel hemocyanin-derived antimicrobial peptide from shrimp Litopenaeus vannamei","volume":"50","author":"Yang","year":"2018","journal-title":"Amino Acids"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"D1154","DOI":"10.1093\/nar\/gkt1157","article-title":"Camp: Collection of sequences and structures of antimicrobial peptides","volume":"42","author":"Waghu","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"D774","DOI":"10.1093\/nar\/gkp1021","article-title":"Camp: A useful resource for research on antimicrobial peptides","volume":"38","author":"Thomas","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"D1087","DOI":"10.1093\/nar\/gkv1278","article-title":"Apd3: The antimicrobial peptide database as a tool for research and education","volume":"44","author":"Wang","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.actbio.2018.01.009","article-title":"Central \u03b2-turn increases the cell selectivity of imperfectly amphipathic \u03b1-helical peptides","volume":"69","author":"Shao","year":"2018","journal-title":"Acta Biomater."},{"key":"ref_56","first-page":"369","article-title":"Hydrophobic parameters ii of amino acid side-chains from the partitioning of n-acetyl-amino acid amides","volume":"18","author":"Fauchere","year":"1983","journal-title":"Eur. J. Med. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.future.2019.04.011","article-title":"I-tasser gateway: A protein structure and function prediction server powered by xsede","volume":"99","author":"Zheng","year":"2019","journal-title":"Future Gener. Comput. Syst."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Lv, Y., Wang, J., Gao, H., Wang, Z., Dong, N., Ma, Q., and Shan, A. (2014). Antimicrobial properties and membrane-active mechanism of a potential \u03b1-helical antimicrobial derived from cathelicidin pmap-36. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0086364"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Jindal, H.M., Le, C.F., Mohd Yusof, M.Y., Velayuthan, R.D., Lee, V.S., Zain, S.M., Isa, D.M., and Sekaran, S.D. (2015). Antimicrobial activity of novel synthetic peptides derived from indolicidin and ranalexin against Streptococcus pneumoniae. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0128532"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Mao, F., Bao, Y., Wong, N.K., Huang, M., Liu, K., Zhang, X., Yang, Z., Yi, W., Shu, X., and Xiang, Z. (2021). Large-scale plasma peptidomic profiling reveals a novel, nontoxic, Crassostrea hongkongensis-derived antimicrobial peptide against foodborne pathogens. Mar. Drugs, 19.","DOI":"10.3390\/md19080420"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"100127","DOI":"10.1016\/j.mtbio.2021.100127","article-title":"Construction of multifunctional porcine acellular dermal matrix hydrogel blended with vancomycin for hemorrhage control, antibacterial action, and tissue repair in infected trauma wounds","volume":"12","author":"Cai","year":"2021","journal-title":"Mater. Today Bio"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"3077","DOI":"10.3390\/md11083077","article-title":"Double strand breaks and cell-cycle arrest induced by the cyanobacterial toxin cylindrospermopsin in hepg2 cells","volume":"11","author":"Alja","year":"2013","journal-title":"Mar. Drugs"}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/20\/7\/459\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:52:11Z","timestamp":1760140331000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/20\/7\/459"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,16]]},"references-count":62,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["md20070459"],"URL":"https:\/\/doi.org\/10.3390\/md20070459","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,16]]}}}