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Here, we modified a 21 kDa fucoidan (FucA) with gallic acid (GA) using the redox method, to potentiate its antioxidant\/protective capacity on pre-osteoblast-like cells (MC3T3) against oxidative stress. The 20 kDa FucA-GA contains 37 \u00b1 3.0 mg GA per gram of FucA. FucA-GA was the most efficient antioxidant agent in terms of total antioxidant capacity (2.5 times), reducing power (five times), copper chelation (three times), and superoxide radical scavenging (2 times). Exposure of MC3T3 cells to H2O2 increased ROS levels and activated caspase-3 along with caspase-9. In addition, the cell viability decreased approximately 80%. FucA-GA also provided the most effective protection against oxidative damage caused by H2O2. Treatment with FucA-GA (1.0 mg\/mL) increased cell viability (~80%) and decreased intracellular ROS (100%) and caspase activation (~80%). In addition, Fuc-GA (0.1 mg\/mL) abolished H2O2-induced oxidative stress in zebra fish embryos. Overall, FucA-GA protected MC3T3 cells from oxidative stress and could represent a possible adjuvant for the treatment of bone fragility by counteracting oxidative phenomena.<\/jats:p>","DOI":"10.3390\/md20080490","type":"journal-article","created":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T02:06:42Z","timestamp":1659319602000},"page":"490","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2345-8108","authenticated-orcid":false,"given":"Keylla Dayanne Coelho Marinho","family":"de Melo","sequence":"first","affiliation":[{"name":"Postgraduate Programe Health Sciences, Federal University of Rio Grande do Norte (UFRN), Natal 59.078-970, RN, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas dos Santos","family":"Lisboa","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Federal University of Rio Grande do Norte, Natal 59.078-970, RN, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4338-6882","authenticated-orcid":false,"given":"Moacir Fernandes","family":"Queiroz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Federal University of Rio Grande do Norte, Natal 59.078-970, RN, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2022-5659","authenticated-orcid":false,"given":"Weslley Souza","family":"Paiva","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Federal University of Rio Grande do Norte, Natal 59.078-970, RN, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina","family":"Luchiari","sequence":"additional","affiliation":[{"name":"Laboratory of Ornamental Fish, Department of Physiology and Behavior, Bioscience Center, Federal University of Rio Grande do Norte, Natal 59.078-970, RN, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5109-1991","authenticated-orcid":false,"given":"Rafael Barros Gomes","family":"Camara","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Federal University of Rio Grande do Norte, Natal 59.078-970, RN, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro Silva","family":"Costa","sequence":"additional","affiliation":[{"name":"Federal Institute of Education, Science and Technology of Rio Grande do Norte, Canguaretama 59.190-000, RN, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2252-1221","authenticated-orcid":false,"given":"Hugo Alexandre Oliveira","family":"Rocha","sequence":"additional","affiliation":[{"name":"Postgraduate Programe Health Sciences, Federal University of Rio Grande do Norte (UFRN), Natal 59.078-970, RN, Brazil"},{"name":"Department of Biochemistry, Federal University of Rio Grande do Norte, Natal 59.078-970, RN, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Luthuli, S., Wu, S., Cheng, Y., Xiaoli, Z., Wu, M., and Tong, H. 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