{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T15:22:17Z","timestamp":1776352937915,"version":"3.51.2"},"reference-count":44,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T00:00:00Z","timestamp":1664150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MICINN","award":["PID2019-105010RB-I00"],"award-info":[{"award-number":["PID2019-105010RB-I00"]}]},{"name":"MICINN","award":["UMA18-FEDERJA-220"],"award-info":[{"award-number":["UMA18-FEDERJA-220"]}]},{"name":"MICINN","award":["PY20_00257"],"award-info":[{"award-number":["PY20_00257"]}]},{"name":"MICINN","award":["IJC2018-037657-I"],"award-info":[{"award-number":["IJC2018-037657-I"]}]},{"name":"Andalusian Government","award":["PID2019-105010RB-I00"],"award-info":[{"award-number":["PID2019-105010RB-I00"]}]},{"name":"Andalusian Government","award":["UMA18-FEDERJA-220"],"award-info":[{"award-number":["UMA18-FEDERJA-220"]}]},{"name":"Andalusian Government","award":["PY20_00257"],"award-info":[{"award-number":["PY20_00257"]}]},{"name":"Andalusian Government","award":["IJC2018-037657-I"],"award-info":[{"award-number":["IJC2018-037657-I"]}]},{"name":"group BIO 267 (Andalusian Government)","award":["PID2019-105010RB-I00"],"award-info":[{"award-number":["PID2019-105010RB-I00"]}]},{"name":"group BIO 267 (Andalusian Government)","award":["UMA18-FEDERJA-220"],"award-info":[{"award-number":["UMA18-FEDERJA-220"]}]},{"name":"group BIO 267 (Andalusian Government)","award":["PY20_00257"],"award-info":[{"award-number":["PY20_00257"]}]},{"name":"group BIO 267 (Andalusian Government)","award":["IJC2018-037657-I"],"award-info":[{"award-number":["IJC2018-037657-I"]}]},{"name":"Spanish Ministry of Science and Innovation","award":["PID2019-105010RB-I00"],"award-info":[{"award-number":["PID2019-105010RB-I00"]}]},{"name":"Spanish Ministry of Science and Innovation","award":["UMA18-FEDERJA-220"],"award-info":[{"award-number":["UMA18-FEDERJA-220"]}]},{"name":"Spanish Ministry of Science and Innovation","award":["PY20_00257"],"award-info":[{"award-number":["PY20_00257"]}]},{"name":"Spanish Ministry of Science and Innovation","award":["IJC2018-037657-I"],"award-info":[{"award-number":["IJC2018-037657-I"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>(+)-Aeroplysinin-1 (Apl-1) is a brominated compound isolated from the marine sponge Aplysina aerophoba that exhibits pleiotropic bioactive effects, impairing cell growth in cancer cells, inhibiting angiogenesis in vitro and in vivo and modulating the redox status of different cell types, among other reported activities. In addition to the aforementioned effects, the anti-inflammatory potential of this natural compound was explored in previous work of our laboratory, but the mechanism of action underlying this effect was not described. In this work, we delve into the anti-inflammatory effect of Apl-1 in the context of vascular endothelial cells in vitro, providing new data regarding the molecular mechanism underlying this activity. The characterization of the mechanism of action points to an inhibitory effect of Apl-1 on the NF-\u03baB pathway, one of the main axes involved in endothelial response during inflammatory events. Our results show that Apl-1 can inhibit the expression of pro-inflammatory genes in tumor necrosis factor alpha (TNF-\u03b1)- and lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells (HUVECs), targeting the nuclear factor kappa B subunit (NF-\u03baB) pathway through a mechanism of action involving the inhibition of I kappa B kinase (IKK) complex phosphorylation and RelA\/p65 nuclear import. In addition, Apl-1 prevented the phosphorylation of Akt induced by TNF-\u03b1 in HUVECs, probably supporting the inhibitory effect of this compound in the NF-\u03baB pathway. Experimental evidence reported in this work opens the door to the potential pharmacological use of this compound as an anti-inflammatory agent in diseases that course with a pathological endothelial response to inflammation, such as atherosclerosis.<\/jats:p>","DOI":"10.3390\/md20100605","type":"journal-article","created":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T01:51:49Z","timestamp":1664329909000},"page":"605","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Sponge-Derived Brominated Compound Aeroplysinin-1 Impairs the Endothelial Inflammatory Response through Inhibition of the NF-\u03baB Pathway"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2396-3323","authenticated-orcid":false,"given":"Isabel","family":"Vidal","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, 29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga y Plataforma en Nanomedicina, IBIMA Plataforma BIONAND, 29590 M\u00e1laga, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Castilla","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, 29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga y Plataforma en Nanomedicina, IBIMA Plataforma BIONAND, 29590 M\u00e1laga, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana D\u00e1cil","family":"Marrero","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, 29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga y Plataforma en Nanomedicina, IBIMA Plataforma BIONAND, 29590 M\u00e1laga, Spain"},{"name":"CIBER de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6932-9619","authenticated-orcid":false,"given":"In\u00e9s","family":"Bravo-Ruiz","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, 29071 M\u00e1laga, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0412-3035","authenticated-orcid":false,"given":"Manuel","family":"Bernal","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, 29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga y Plataforma en Nanomedicina, IBIMA Plataforma BIONAND, 29590 M\u00e1laga, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inmaculada","family":"Manrique","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, 29071 M\u00e1laga, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6419-8867","authenticated-orcid":false,"given":"Ana","family":"R. Quesada","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, 29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga y Plataforma en Nanomedicina, IBIMA Plataforma BIONAND, 29590 M\u00e1laga, Spain"},{"name":"CIBER de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7275-6462","authenticated-orcid":false,"given":"Miguel \u00c1ngel","family":"Medina","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, 29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga y Plataforma en Nanomedicina, IBIMA Plataforma BIONAND, 29590 M\u00e1laga, Spain"},{"name":"CIBER de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3927-3877","authenticated-orcid":false,"given":"Beatriz","family":"Mart\u00ednez-Poveda","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, 29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga y Plataforma en Nanomedicina, IBIMA Plataforma BIONAND, 29590 M\u00e1laga, Spain"},{"name":"CIBER de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1007\/s11883-022-01023-9","article-title":"More than Just a Monolayer: The Multifaceted Role of Endothelial Cells in the Pathophysiology of Atherosclerosis","volume":"24","author":"Mussbacher","year":"2022","journal-title":"Curr. 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