{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:14:40Z","timestamp":1787242480399,"version":"build-2736575974"},"reference-count":55,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,27]],"date-time":"2023-01-27T00:00:00Z","timestamp":1674777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Teagasc","award":["754380"],"award-info":[{"award-number":["754380"]}]},{"DOI":"10.13039\/501100000780","name":"Marie Sk\u0142odowska-Curie","doi-asserted-by":"publisher","award":["754380"],"award-info":[{"award-number":["754380"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Bioactive peptides range in size from 2\u201330 amino acids and may be derived from any protein-containing biomass using hydrolysis, fermentation or high-pressure processing. Pro-peptides or cryptides result in shorter peptide sequences following digestion and may have enhanced bioactivity. Previously, we identified a protein hydrolysate generated from Laminaria digitata that inhibited ACE-1 in vitro and had an ACE-1 IC50 value of 590 \u00b5g\/mL compared to an ACE-1 IC50 value of 500 \u00b5g\/mL (~2.3 \u00b5M) observed for the anti-hypertensive drug Captopril\u00a9. A number of peptide sequences (130 in total) were identified using mass spectrometry from a 3 kDa permeate of this hydrolysate. Predicted bioactivities for these peptides were determined using an in silico strategy previously published by this group utilizing available databases including Expasy peptide cutter, BIOPEP and Peptide Ranker. Peptide sequences YIGNNPAKGGLF and IGNNPAKGGLF had Peptide Ranker scores of 0.81 and 0.80, respectively, and were chemically synthesized. Synthesized peptides were evaluated for ACE-1 inhibitory activity in vitro and were found to inhibit ACE-1 by 80 \u00b1 8% and 91 \u00b1 16%, respectively. The observed ACE-1 IC50 values for IGNNPAKGGLF and YIGNNPAKGGLF were determined as 174.4 \u00b5g\/mL and 133.1 \u00b5g\/mL. Both peptides produced sequences following simulated digestion with the potential to inhibit Dipeptidyl peptidase IV (DPP-IV).<\/jats:p>","DOI":"10.3390\/md21020090","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T11:19:53Z","timestamp":1675077593000},"page":"90","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Identification of Bioactive Peptides from a Laminaria digitata Protein Hydrolysate Using In Silico and In Vitro Methods to Identify Angiotensin-1-Converting Enzyme (ACE-1) Inhibitory Peptides"],"prefix":"10.3390","volume":"21","author":[{"given":"Diane","family":"Purcell","sequence":"first","affiliation":[{"name":"Food BioSciences Department, Teagasc Food Research Centre, Ashtown, Dublin 15, D15 DY05 Dublin, Ireland"},{"name":"Cawthron Institute, 98 Halifax Street, Nelson 7010, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0854-9711","authenticated-orcid":false,"given":"Michael A.","family":"Packer","sequence":"additional","affiliation":[{"name":"Cawthron Institute, 98 Halifax Street, Nelson 7010, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Hayes","sequence":"additional","affiliation":[{"name":"Food BioSciences Department, Teagasc Food Research Centre, Ashtown, Dublin 15, D15 DY05 Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1146\/annurev.bi.51.070182.001435","article-title":"Enzymes of the renin-angiotensin system and their inhibitors","volume":"51","author":"Ondetti","year":"1982","journal-title":"Annu. 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