{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T13:44:37Z","timestamp":1783518277756,"version":"3.55.0"},"reference-count":48,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T00:00:00Z","timestamp":1681948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Program of I + D Activities of the Autonomous Community of Madrid, Spain","award":["S2013\/ABI-278"],"award-info":[{"award-number":["S2013\/ABI-278"]}]},{"name":"Program of I + D Activities of the Autonomous Community of Madrid, Spain","award":["PID2022-136535OB-I00"],"award-info":[{"award-number":["PID2022-136535OB-I00"]}]},{"name":"European Structural Fund","award":["S2013\/ABI-278"],"award-info":[{"award-number":["S2013\/ABI-278"]}]},{"name":"European Structural Fund","award":["PID2022-136535OB-I00"],"award-info":[{"award-number":["PID2022-136535OB-I00"]}]},{"name":"Ministerio de Ciencia e Innovaci\u00f3n and Agencia Estatal de Investigaci\u00f3n (Spanish Government)","award":["S2013\/ABI-278"],"award-info":[{"award-number":["S2013\/ABI-278"]}]},{"name":"Ministerio de Ciencia e Innovaci\u00f3n and Agencia Estatal de Investigaci\u00f3n (Spanish Government)","award":["PID2022-136535OB-I00"],"award-info":[{"award-number":["PID2022-136535OB-I00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Arthrospira platensis biomass is a sustainable source of bioactive products for the food, cosmetic, and medicine industries. As well as primary metabolites, different secondary metabolites can be obtained via distinct enzymatic degradation of biomass. In this work, different hydrophilic extracts were obtained after treating the biomass with: (i) a serine endo-peptidase (Alcalase\u00ae), (ii) a mixture of amino-, dipeptidyl-, and endo-peptidases (Flavourzyme\u00ae), (iii) a mixture of endo-1,3(4)-\u03b2-glucanase and an endo-1,4-xylanase, and \u03b2-glucanase (Ultraflo\u00ae), and (iv) an exo-1,3-glucanase (Vinoflow\u00ae) (all the enzymes from Novozymes A\/S (bagsvaerd, Denmark)); with subsequent extraction of the biocomponents with an isopropanol\/hexane mixture. The composition of each aqueous phase extract (in terms of amino acids, peptides, oligo-elements, carbohydrates, and phenols) and their in vitro functional properties were compared. The conditions described in this work using the enzyme Alcalase\u00ae permits the extraction of eight distinctive peptides. This extract is 7.3 times more anti-hypertensive, 106 times more anti-hypertriglyceridemic, 26 times more hypocholesterolemic, has 4.4 times more antioxidant activities, and has 2.3 times more phenols, than the extract obtained without any prior enzyme biomass digestion. Alcalase\u00ae extract is an advantageous product with potential application in functional food, pharmaceutics, and cosmetics.<\/jats:p>","DOI":"10.3390\/md21040255","type":"journal-article","created":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T01:33:43Z","timestamp":1682040823000},"page":"255","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Preparation and Characterization of a Multicomponent Arthrospira platensis Biomass Hydrolysate with Superior Anti-Hypertensive, Anti-Hyperlipidemic and Antioxidant Activities via Selective Proteolysis"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4813-4142","authenticated-orcid":false,"given":"Cristina","family":"Otero","sequence":"first","affiliation":[{"name":"Department of Biocatalysis, Institute of Catalysis and Petroleochemistry, Consejo Superior de Investigaciones Cient\u00edficas-CSIC, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos M.","family":"Verdasco-Mart\u00edn","sequence":"additional","affiliation":[{"name":"Department of Biocatalysis, Institute of Catalysis and Petroleochemistry, Consejo Superior de Investigaciones Cient\u00edficas-CSIC, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1080\/09670262.2017.1362593","article-title":"The possible roles of algae in restricting the increase in atmospheric CO2 and global temperature","volume":"52","author":"Raven","year":"2017","journal-title":"Eur. 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