{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T20:25:41Z","timestamp":1774729541357,"version":"3.50.1"},"reference-count":75,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T00:00:00Z","timestamp":1687996800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Brazilian National Council for Scientific and Technological Development\u2014CNPq","doi-asserted-by":"publisher","award":["309771\/2019-6"],"award-info":[{"award-number":["309771\/2019-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Federal University of Rio Grande do Norte","award":["309771\/2019-6"],"award-info":[{"award-number":["309771\/2019-6"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>A blend refers to the combination of two or more components to achieve properties that are superior to those found in the individual products used for their production. Gracilaria birdiae agaran (SPGb) and chromium picolinate (ChrPic) are both antioxidant agents. However, there is no documentation of blends that incorporate agarans and ChrPic. Hence, the objective of this study was to generate blends containing SPGb and ChrPic that exhibit enhanced antioxidant activity compared to SPGb or ChrPic alone. ChrPic was commercially acquired, while SPGb was extracted from the seaweed. Five blends (B1; B2; B3; B4; B5) were produced, and tests indicated B5 as the best antioxidant blend. B5 was not cytotoxic or genotoxic. H2O2 (0.6 mM) induced toxicity in fibroblasts (3T3), and this effect was abolished by B5 (0.05 mg\u00b7mL\u22121); neither ChrPic nor SPGb showed this effect. The cells also showed no signs of toxicity when exposed to H2O2 after being incubated with B5 and ChrPic for 24 h. In another experiment, cells were incubated with H2O2 and later exposed to SPGb, ChrPic, or B5. Again, SPGb was not effective, while cells exposed to ChrPic and B5 reduced MTT by 100%. The data demonstrated that B5 has activity superior to SPGb and ChrPic and points to B5 as a product to be used in future in vivo tests to confirm its antioxidant action. It may also be indicated as a possible nutraceutical agent.<\/jats:p>","DOI":"10.3390\/md21070388","type":"journal-article","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T01:02:41Z","timestamp":1688086961000},"page":"388","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Blend Consisting of Agaran from Seaweed Gracilaria birdiae and Chromium Picolinate Is a Better Antioxidant Agent than These Two Compounds Alone"],"prefix":"10.3390","volume":"21","author":[{"given":"Yara","family":"Campanelli-Morais","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioquimica e Biologia Molecular, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia Haynara Ferreira","family":"Silva","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioquimica e Biologia Molecular, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3664-4755","authenticated-orcid":false,"given":"Marina Rocha do Nascimento","family":"Dantas","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioquimica e Biologia Molecular, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7939-8135","authenticated-orcid":false,"given":"Diego Araujo","family":"Sabry","sequence":"additional","affiliation":[{"name":"Dapartamento de Bioqu\u00edmica, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7420-168X","authenticated-orcid":false,"given":"Guilherme Lanzi","family":"Sassaki","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Biologia Molecular, Setor de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Paran\u00e1 (UFPR), Curitiba 81531-980, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8333-3016","authenticated-orcid":false,"given":"Susana Margarida Gomes","family":"Moreira","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioquimica e Biologia Molecular, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-900, Brazil"},{"name":"Departamento de Biologia Celular e Gen\u00e9tica, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2252-1221","authenticated-orcid":false,"given":"Hugo Alexandre Oliveira","family":"Rocha","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioquimica e Biologia Molecular, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-900, Brazil"},{"name":"Dapartamento de Bioqu\u00edmica, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/raq.12580","article-title":"The underexplored potential of green macroalgae in aquaculture","volume":"14","author":"Moreira","year":"2022","journal-title":"Rev. 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