{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T05:08:42Z","timestamp":1792559322203,"version":"4.5.0"},"reference-count":38,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T00:00:00Z","timestamp":1693267200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"publisher","award":["2019YFD0902004"],"award-info":[{"award-number":["2019YFD0902004"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"publisher","award":["2019KCXTD011"],"award-info":[{"award-number":["2019KCXTD011"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangdong Innovation Team of Seafood Green Processing Technology","award":["2019YFD0902004"],"award-info":[{"award-number":["2019YFD0902004"]}]},{"name":"Guangdong Innovation Team of Seafood Green Processing Technology","award":["2019KCXTD011"],"award-info":[{"award-number":["2019KCXTD011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Anserine is a naturally occurring histidine dipeptide with significant antioxidant activities. This study aimed to investigate the preventive mechanism of anserine on tert-butyl hydroperoxide (TBHP)-induced liver damage in a normal human liver cell line (L-02 cells). The L-02 cells were pretreated with anserine (10, 20, and 40 mmol\/L) and then induced with 400 \u03bcmol\/L of TBHP for 4 h. The results showed that the survival rates of L-02 cells and the contents of GSH were significantly increased with the pretreatment of anserine; the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the extracellular fluid were sharply decreased; and the formation of reactive oxygen species (ROS), nuclear fragmentation, and apoptosis were significantly inhibited. In addition, anserine could bind to the Kelch domain of Kelch-like ECH-associated protein 1 (Keap1) with a binding force of \u22127.2 kcal\/mol; the protein expressions of nuclear factor-erythroid 2-related factor-2 (Nrf2), quinone oxidoreductase 1 (NQO1), heme oxygenase-1 (HO-1), and Bcl-2 were upregulated by anserine in TBHP-induced L-02 cells, with the downregulation of p-JNK and caspase-3. In conclusion, anserine might alleviated liver injury in L-02 cells via regulating related proteins in the Keap1-Nrf2 and JNK-Caspase-3 signaling pathways.<\/jats:p>","DOI":"10.3390\/md21090477","type":"journal-article","created":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T10:09:49Z","timestamp":1693390189000},"page":"477","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Preventive Mechanism of Anserine on Tert-Butyl Hydroperoxide-Induced Liver Injury in L-02 Cells via Regulating the Keap1-Nrf2 and JNK-Caspase-3 Signaling Pathways"],"prefix":"10.3390","volume":"21","author":[{"given":"Ming","family":"Chen","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwu","family":"Ji","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China"},{"name":"Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang 524088, China"},{"name":"Guangdong Provincial Engineering Technology Research Center of Marine Food, Zhanjiang 524088, China"},{"name":"Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenkui","family":"Song","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7030-6249","authenticated-orcid":false,"given":"Di","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiming","family":"Su","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1775-8470","authenticated-orcid":false,"given":"Shucheng","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China"},{"name":"Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang 524088, China"},{"name":"Guangdong Provincial Engineering Technology Research Center of Marine Food, Zhanjiang 524088, China"},{"name":"Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China"},{"name":"Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1038\/s41573-021-00267-5","article-title":"Author Correction: Targeting oxidative stress in disease: Promise and limitations of antioxidant therapy","volume":"20","author":"Forman","year":"2021","journal-title":"Nat. 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