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This study investigated the bioactivity of extracts from brown algae Fucus vesiculosus against rotavirus, the worldwide leading cause of acute gastroenteritis in infants and young children. Moreover, one of the extracts was tested against four foodborne bacteria: Campylobacter jejuni, Escherichia coli, Salmonella Typhimurium, and Listeria monocytogenes, and the non-pathogenic: E. coli K12. In vitro tests using MA104 cells revealed that both whole algae extracts and crude fucoidan precipitates neutralized rotavirus in a dose-responsive manner. The maximum neutralization activity was observed when the rotavirus was incubated with 100 \u03bcg mL\u22121 of the hydrochloric acid-obtained crude fucoidan (91.8%), although crude fucoidan extracted using citric acid also demonstrated high values (89.5%) at the same concentration. Furthermore, molecular weight fractionation of extracts decreased their antirotaviral activity and high molecular weight fractions exhibited higher activity compared to those of lower molecular weight. A seaweed extract with high antirotaviral activity was also found to inhibit the growth of C. jejuni, S. Typhimurium, and L. monocytogenes at a concentration of 0.2 mg mL\u22121. Overall, this study expands the current knowledge regarding the antimicrobial mechanisms of action of extracts from F. vesiculosus.<\/jats:p>","DOI":"10.3390\/md21090478","type":"journal-article","created":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T10:09:49Z","timestamp":1693390189000},"page":"478","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Bioactivity of Fucoidan-Rich Extracts from Fucus vesiculosus against Rotavirus and Foodborne Pathogens"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4597-3888","authenticated-orcid":false,"given":"Dimitra","family":"Graikini","sequence":"first","affiliation":[{"name":"Departamento de Producci\u00f3n Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain"},{"name":"Instituto Agroalimentario de Arag\u00f3n IA2 (UNIZAR-CITA), 50013 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6378-9334","authenticated-orcid":false,"given":"Arturo B.","family":"Soro","sequence":"additional","affiliation":[{"name":"Foodborne Pathogens Unit, Department of Infectious Diseases in Humans, Sciensano, 1050 Brussels, Belgium"},{"name":"Teagasc Ashtown Food Research Centre, D15 DY05 Dublin, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5019-8348","authenticated-orcid":false,"given":"Saravana P.","family":"Sivagnanam","sequence":"additional","affiliation":[{"name":"Teagasc Ashtown Food Research Centre, D15 DY05 Dublin, Ireland"},{"name":"Department of Biological Sciences, Munster Technological University, Bishopstown, T12P928 Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brijesh K.","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Teagasc Ashtown Food Research Centre, D15 DY05 Dublin, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5964-823X","authenticated-orcid":false,"given":"Lourdes","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain"},{"name":"Instituto Agroalimentario de Arag\u00f3n IA2 (UNIZAR-CITA), 50013 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/S1473-3099(17)30276-1","article-title":"Estimates of global, regional, and national morbidity, mortality, and aetiologies of diarrhoeal diseases: A systematic analysis for the Global Burden of Disease Study 2015","volume":"17","author":"Troeger","year":"2017","journal-title":"Lancet Infect. 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