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District Science and Technology Plan Project","award":["82022067"],"award-info":[{"award-number":["82022067"]}]},{"name":"Nansha District Science and Technology Plan Project","award":["22037006"],"award-info":[{"award-number":["22037006"]}]},{"name":"Nansha District Science and Technology Plan Project","award":["2019BT02Y262"],"award-info":[{"award-number":["2019BT02Y262"]}]},{"name":"Nansha District Science and Technology Plan Project","award":["SZBL2021080601006"],"award-info":[{"award-number":["SZBL2021080601006"]}]},{"name":"Nansha District Science and Technology Plan Project","award":["NHXX2019SW0101"],"award-info":[{"award-number":["NHXX2019SW0101"]}]},{"name":"Nansha District Science and Technology Plan Project","award":["NSJL202102"],"award-info":[{"award-number":["NSJL202102"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Marine symbiotic and epiphyte microorganisms are sources of bioactive or structurally novel natural products. Metabolic blockade-based genome mining has been proven to be an effective strategy to accelerate the discovery of natural products from both terrestrial and marine microorganisms. Here, the metabolic blockade-based genome mining strategy was applied to the discovery of other metabolites in a sea anemone-associated Streptomyces sp. S1502. We constructed a mutant Streptomyces sp. S1502\/\u0394stp1 that switched to producing the atypical angucyclines WS-5995 A\u2013E, among which WS-5995 E is a new compound. A biosynthetic gene cluster (wsm) of the angucyclines was identified through gene knock-out and heterologous expression studies. The biosynthetic pathways of WS-5995 A\u2013E were proposed, the roles of some tailoring and regulatory genes were investigated, and the biological activities of WS-5995 A\u2013E were evaluated. WS-5995 A has significant anti-Eimeria tenell activity with an IC50 value of 2.21 \u03bcM. The production of antibacterial streptopyrroles and anticoccidial WS-5995 A\u2013E may play a protective role in the mutual relationship between Streptomyces sp. S1502 and its host.<\/jats:p>","DOI":"10.3390\/md22050195","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T08:08:32Z","timestamp":1714032512000},"page":"195","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Metabolic Blockade-Based Genome Mining of Sea Anemone-Associated Streptomyces sp. S1502 Identifies Atypical Angucyclines WS-5995 A\u2013E: Isolation, Identification, Biosynthetic Investigation, and Bioactivities"],"prefix":"10.3390","volume":"22","author":[{"given":"Yuyang","family":"Wang","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Haizhu District, Guangzhou 510301, China"},{"name":"College of Oceanology, University of Chinese Academy of Sciences, Qingdao 266400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Zhou","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Haizhu District, Guangzhou 510301, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoting","family":"Pan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory of Avian Influenza and Other Major Poultry Diseases Prevention and Control, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangjun","family":"Liao","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Institute of Laboratory Medicine, Guangdong Medical University, Dongguan 523808, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanshan","family":"Qi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory of Avian Influenza and Other Major Poultry Diseases Prevention and Control, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingfei","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory of Avian Influenza and Other Major Poultry Diseases Prevention and Control, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Institute of Laboratory Medicine, Guangdong Medical University, Dongguan 523808, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7712-8027","authenticated-orcid":false,"given":"Jianhua","family":"Ju","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Haizhu District, Guangzhou 510301, China"},{"name":"College of Oceanology, University of Chinese Academy of Sciences, Qingdao 266400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0641-7291","authenticated-orcid":false,"given":"Junying","family":"Ma","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Haizhu District, Guangzhou 510301, China"},{"name":"College of Oceanology, University of Chinese Academy of Sciences, Qingdao 266400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1021\/acs.jnatprod.3c00202","article-title":"Six sets of aromatic polyketides differing in size and shape derive from a single biosynthetic gene cluster","volume":"86","author":"Xu","year":"2023","journal-title":"J. Nat. Prod."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Dong, Y., Ding, W., Sun, C., Ji, X., Ling, C., Zhou, Z., Chen, Z., Chen, X., and Ju, J. (2020). Julichrome monomers from marine gastropod mollusk-associated Streptomyces and stereochemical revision of julichromes Q(3\u00b75) and Q(3\u00b73). Chem. Biodivers., 17.","DOI":"10.1002\/cbdv.202000057"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Ji, C.H., Je, H.W., Kim, H., and Kang, H.S. (2024). Promoter engineering of natural product biosynthetic gene clusters in actinomycetes: Concepts and applications. Nat. Prod. Rep.","DOI":"10.1039\/D3NP00049D"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1039\/C8NP00091C","article-title":"Heterologous expression of bacterial natural product biosynthetic pathways","volume":"36","author":"Huo","year":"2019","journal-title":"Nat. Prod. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4874","DOI":"10.1021\/acs.orglett.3c01683","article-title":"Genome-directed discovery of bicyclic cinnamoyl-containing nonribosomal peptides with anticoronaviral activity from Streptomyces griseus","volume":"25","author":"Li","year":"2023","journal-title":"Org. Lett."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.apsb.2020.07.020","article-title":"Genome mining combined metabolic shunting and OSMAC strategy of an endophytic fungus leads to the production of diverse natural products","volume":"11","author":"Wei","year":"2021","journal-title":"Acta Pharm. Sin. B"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5535","DOI":"10.1021\/acs.orglett.2c01957","article-title":"Metabolic blockade-based genome mining reveals lipochain-linked dihydro-beta-alanine synthetases involved in autucedine biosynthesis","volume":"24","author":"Zhang","year":"2022","journal-title":"Org. Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"133148","DOI":"10.1016\/j.tet.2022.133148","article-title":"Metabolic blockade-based genome mining of Streptomyces cacaoi SCSIO 68063: Isolation and identification of BE-18257 and pentaminomycin analogues","volume":"130","author":"Wang","year":"2023","journal-title":"Tetrahedron"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1039\/C1NP00068C","article-title":"Angucyclines: Biosynthesis, mode-of-action, new natural products, and synthesis","volume":"29","author":"Kharel","year":"2012","journal-title":"Nat. Prod. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3780","DOI":"10.1021\/ja0680515","article-title":"Multi-oxygenase complexes of the gilvocarcin and jadomycin biosyntheses","volume":"129","author":"Kharel","year":"2007","journal-title":"J. Am. Chem. Soc."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"18181","DOI":"10.1021\/ja3081154","article-title":"Baeyer-Villiger C-C bond cleavage reaction in gilvocarcin and jadomycin biosynthesis","volume":"134","author":"Tibrewal","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2336","DOI":"10.1021\/acs.jnatprod.1c00461","article-title":"Discovery of an unexpected 1,4-oxazepine-linked seco-fluostatin heterodimer by inactivation of the oxidoreductase-encoding gene flsP","volume":"84","author":"Huang","year":"2021","journal-title":"J. Nat. Prod."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"11019","DOI":"10.1021\/acs.joc.0c02517","article-title":"Inactivation of flavoenzyme-encoding gene flsO1 in fluostatin biosynthesis leads to diversified angucyclinone derivatives","volume":"86","author":"Yang","year":"2021","journal-title":"J. Org. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"5386","DOI":"10.1038\/s41467-022-33131-0","article-title":"Biochemical and structural insights of multifunctional flavin-dependent monooxygenase FlsO1-catalyzed unexpected xanthone formation","volume":"13","author":"Yang","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"12402","DOI":"10.1021\/ja305113d","article-title":"Roles of the synergistic reductive O-methyltransferase GilM and of O-methyltransferase GilMT in the gilvocarcin biosynthetic pathway","volume":"134","author":"Tibrewal","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"11902","DOI":"10.1021\/ja104550p","article-title":"Fluostatins produced by the heterologous expression of a TAR reassembled environmental DNA derived type II PKS gene cluster","volume":"132","author":"Feng","year":"2010","journal-title":"J. Am. Chem. Soc."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1039\/C6NP00002A","article-title":"Native and engineered promoters in natural product discovery","volume":"33","author":"Myronovskyi","year":"2016","journal-title":"Nat. Prod. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5324","DOI":"10.1021\/acs.orglett.5b02683","article-title":"Heterologous expression of fluostatin gene cluster leads to a bioactive heterodimer","volume":"17","author":"Yang","year":"2015","journal-title":"Org. Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1021\/acs.jnatprod.7b00652","article-title":"Reconstitution of kinamycin biosynthesis within the heterologous host Streptomyces albus J1074","volume":"81","author":"Liu","year":"2018","journal-title":"J. Nat. Prod."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e202202286","DOI":"10.1002\/anie.202202286","article-title":"Investigation of the molecular landscape of bacterial aromatic polyketides by global analysis of type II polyketide synthases","volume":"61","author":"Chen","year":"2022","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7164\/antibiotics.53.1","article-title":"Novel streptopyrroles from Streptomyces rimosus with bacterial protein histidine kinase inhibitory and antimicrobial activities","volume":"53","author":"Trew","year":"2000","journal-title":"J. Antibiot."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"W276","DOI":"10.1093\/nar\/gkz282","article-title":"AutoMLST: An automated web server for generating multi-locus species trees highlighting natural product potential","volume":"47","author":"Alanjary","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"W46","DOI":"10.1093\/nar\/gkad344","article-title":"AntiSMASH 7.0: New and improved predictions for detection, regulation, chemical structures and visualisation","volume":"51","author":"Blin","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5390","DOI":"10.1039\/C5OB02599K","article-title":"Nitropyrrole natural products: Isolation, biosynthesis and total synthesis","volume":"14","author":"Ding","year":"2016","journal-title":"Org. Biomol. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"11546","DOI":"10.1021\/jacs.5b04525","article-title":"Structure and substrate sequestration in the pyoluteorin type II peptidyl carrier protein PltL","volume":"137","author":"Jaremko","year":"2015","journal-title":"J. Am. Chem. Soc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3682","DOI":"10.1021\/jacs.0c12415","article-title":"Discovery and biosynthesis of tetrachlorizine reveals enzymatic benzylic dehydrogenation via an ortho-quinone methide","volume":"143","author":"Purdy","year":"2021","journal-title":"J. Am. Chem. Soc."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1021\/acs.jnatprod.8b00753","article-title":"Characterization of the biosynthetic gene vluster for the antibiotic armeniaspirols in Streptomyces armeniacus","volume":"82","author":"Qiao","year":"2019","journal-title":"J. Nat. Prod."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1007\/s00253-009-2254-7","article-title":"WS-5995 B, an antifungal agent inducing differential gene expression in the conifer pathogen Heterobasidion annosum but not in Heterobasidion abietinum","volume":"85","author":"Lehr","year":"2009","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.7164\/antibiotics.33.1107","article-title":"New anticoccidial antibiotics, WS-5995 A and B. I. isolation and characterization","volume":"33","author":"Ikushima","year":"1980","journal-title":"J. Antibiot."},{"key":"ref_30","first-page":"1","article-title":"Construction of Streptomyces lividans SBT5 as an efficient heterologous expression host","volume":"33","author":"Bai","year":"2014","journal-title":"J. Huazhong Agric. Univ."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3772","DOI":"10.1021\/acssynbio.2c00362","article-title":"MGCEP 1.0: A genetic-engineered marine-derived chassis cell for a scaled heterologous expression platform of microbial bioactive metabolites","volume":"11","author":"Yang","year":"2022","journal-title":"ACS Synth. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"van den Belt, M., Gilchrist, C., Booth, T.J., Chooi, Y.H., Medema, M.H., and Alanjary, M. (2023). CAGECAT: The Comparative gene cluster analysis toolbox for rapid search and visualisation of homologous gene clusters. BMC Bioinform., 24.","DOI":"10.1186\/s12859-023-05311-2"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1021\/acssynbio.3c00730","article-title":"Eficient mutasynthesis of \u201cnon-natural\u201d antitubercular ilamycins with low cytotoxicity","volume":"13","author":"Wang","year":"2024","journal-title":"ACs Synth. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1139\/g00-117","article-title":"Melon bacterial artificial chromosome (BAC) library construction using improved methods and identification of clones linked to the locus conferring resistance to melon Fusarium wilt (Fom-2)","volume":"44","author":"Luo","year":"2001","journal-title":"Genome"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.1021\/acschembio.2c00187","article-title":"Bacterial associates of a desert specialist fungus-growing ant antagonize competitors with a nocamycin analog","volume":"17","author":"Hansen","year":"2022","journal-title":"ACS Chem. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"6927","DOI":"10.1021\/acs.orglett.0c02469","article-title":"Elucidation of the tailoring steps in julichrome biosynthesis by marine gastropod mollusk-associated Streptomyces sampsonii SCSIO 054","volume":"22","author":"Ji","year":"2020","journal-title":"Org. Lett."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/22\/5\/195\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:33:01Z","timestamp":1760106781000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/22\/5\/195"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,25]]},"references-count":36,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["md22050195"],"URL":"https:\/\/doi.org\/10.3390\/md22050195","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,25]]}}}