{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:10:09Z","timestamp":1791925809240,"version":"4.3.4"},"reference-count":51,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 research and innovation programme","award":["817806"],"award-info":[{"award-number":["817806"]}]},{"name":"European Union\u2019s Horizon 2020 research and innovation programme","award":["817669"],"award-info":[{"award-number":["817669"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>This study generated bioactive hydrolysates using the enzyme Alcalase and autolysis from mesopelagic fish, including Maurolicus muelleri and Benthosema glaciale. Generated hydrolysates were investigated for their bioactivities using in vitro bioassays, and bioactive peptides were identified using mass spectrometry in active hydrolysates with cyclooxygenase, dipeptidyl peptidase IV and antioxidant activities. In silico analysis was employed to rank identified peptide sequences in terms of overall bioactivity using programmes including Peptide Ranker, PrepAIP, Umami-MRNN and AntiDMPpred. Seven peptides predicted to have anti-inflammatory, anti-type 2 diabetes or Umami potential using in silico strategies were chemically synthesised, and their anti-inflammatory activities were confirmed using in vitro bioassays with COX-1 and COX-2 enzymes. The peptide QCPLHRPWAL inhibited COX-1 and COX-2 by 82.90% (+\/\u22120.54) and 53.84%, respectively, and had a selectivity index greater than 10. This peptide warrants further research as a novel anti-inflammatory\/pain relief peptide. Other peptides with DPP-IV inhibitory and Umami flavours were identified. These offer potential for use as functional foods or topical agents to prevent pain and inflammation.<\/jats:p>","DOI":"10.3390\/md22070297","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T08:57:50Z","timestamp":1719478670000},"page":"297","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Generation, Characterisation and Identification of Bioactive Peptides from Mesopelagic Fish Protein Hydrolysates Using In Silico and In Vitro Approaches"],"prefix":"10.3390","volume":"22","author":[{"given":"Maria","family":"Hayes","sequence":"first","affiliation":[{"name":"Food BioSciences Department, Teagasc Food Research Centre, Ashtown, D15 DY05 Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azza","family":"Naik","sequence":"additional","affiliation":[{"name":"Food BioSciences Department, Teagasc Food Research Centre, Ashtown, D15 DY05 Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1571-5781","authenticated-orcid":false,"given":"Leticia","family":"Mora","sequence":"additional","affiliation":[{"name":"Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (CSIC), Avenue Agust\u00edn Escardino 7, Valencia, 46980 Paterna, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1544-0370","authenticated-orcid":false,"given":"Bruno","family":"I\u00f1arra","sequence":"additional","affiliation":[{"name":"AZTI, Food Research, Basque Research and Technology Alliance (BRTA), Parque Tecnol\u00f3gico de Bizkaia, Astondo Bidea, Edificio 609, 48160 Derio, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3475-3137","authenticated-orcid":false,"given":"Jone","family":"Ibarruri","sequence":"additional","affiliation":[{"name":"AZTI, Food Research, Basque Research and Technology Alliance (BRTA), Parque Tecnol\u00f3gico de Bizkaia, Astondo Bidea, Edificio 609, 48160 Derio, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6715-8492","authenticated-orcid":false,"given":"Carlos","family":"Bald","sequence":"additional","affiliation":[{"name":"AZTI, Food Research, Basque Research and Technology Alliance (BRTA), Parque Tecnol\u00f3gico de Bizkaia, Astondo Bidea, Edificio 609, 48160 Derio, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7504-6439","authenticated-orcid":false,"given":"Thibault","family":"Cariou","sequence":"additional","affiliation":[{"name":"Marine Institute, Fisheries and Ecosystems Advisory, Rinville, Oranmore, H91 R673 Co. Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Reid","sequence":"additional","affiliation":[{"name":"Marine Institute, Fisheries and Ecosystems Advisory, Rinville, Oranmore, H91 R673 Co. Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Gallagher","sequence":"additional","affiliation":[{"name":"Bord Iascaigh Mhara, The Pier, Killybegs, F94 P8YP Co. Donegal, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8655-1222","authenticated-orcid":false,"given":"Ragnhild","family":"Drag\u00f8y","sequence":"additional","affiliation":[{"name":"Department of Marine Biotechnology, Muninbakken 9-13, 9019 Troms\u00f8, Norway"},{"name":"Aker BioMarine, Oksen\u00f8yveien 10, 1366 Lysaker, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Galino","sequence":"additional","affiliation":[{"name":"Health and Biomedicine Department, Leitat Technological Center, Carrer de la Innovaci\u00f3, 2, 08225 Terrassa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Dey\u00e0","sequence":"additional","affiliation":[{"name":"Health and Biomedicine Department, Leitat Technological Center, Carrer de la Innovaci\u00f3, 2, 08225 Terrassa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sissel","family":"Albrektsen","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Feed Technology, Nofima AS, P.O. 5844 Oasen, 5828 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Thoresen","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Feed Technology, Nofima AS, P.O. 5844 Oasen, 5828 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3463-6034","authenticated-orcid":false,"given":"Runar G.","family":"Solstad","sequence":"additional","affiliation":[{"name":"Department of Marine Biotechnology, Muninbakken 9-13, 9019 Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"241","DOI":"10.3354\/meps09363","article-title":"Long-term acoustical observations of the mesopelagic fish Maurolicus muelleri reveal novel and varied vertical migration patterns","volume":"441","author":"Staby","year":"2011","journal-title":"Mar. 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