{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:03:37Z","timestamp":1787166217935,"version":"build-2736575974"},"reference-count":44,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T00:00:00Z","timestamp":1723593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"CONACYT","doi-asserted-by":"publisher","award":["302979"],"award-info":[{"award-number":["302979"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"CONACYT","doi-asserted-by":"publisher","award":["A1-S-9005"],"award-info":[{"award-number":["A1-S-9005"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"name":"PAIPC","award":["302979"],"award-info":[{"award-number":["302979"]}]},{"name":"PAIPC","award":["A1-S-9005"],"award-info":[{"award-number":["A1-S-9005"]}]},{"name":"CONF\u00cdE","award":["302979"],"award-info":[{"award-number":["302979"]}]},{"name":"CONF\u00cdE","award":["A1-S-9005"],"award-info":[{"award-number":["A1-S-9005"]}]},{"name":"SMV","award":["302979"],"award-info":[{"award-number":["302979"]}]},{"name":"SMV","award":["A1-S-9005"],"award-info":[{"award-number":["A1-S-9005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Dengue, caused by the dengue virus (DENV), is a global health threat transmitted by Aedes mosquitoes, resulting in 400 million cases annually. The disease ranges from mild to severe, with potential progression to hemorrhagic dengue. Current research is focused on natural antivirals due to challenges in vector control. This study evaluates the antiviral potential of peptides derived from the microalgae Phaeodactylum tricornutum, known for its bioactive compounds. Microalgae were cultivated under controlled conditions, followed by protein extraction and hydrolysis to produce four peptide fractions. These fractions were assessed for cytotoxicity via the MTT assay and antiviral activity against DENV serotype 2 using flow cytometry and plaque formation assays. The 10\u201330 kDa peptide fraction, at 150 and 300 \u03bcg\/mL concentrations, demonstrated no cytotoxicity and significantly reduced the percentage of infected cells and viral titers. These findings suggest that peptides derived from Phaeodactylum tricornutum exhibit promising antiviral activity against dengue virus serotype 2, potentially contributing to developing new therapeutic approaches for dengue.<\/jats:p>","DOI":"10.3390\/md22080369","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T03:46:36Z","timestamp":1723607196000},"page":"369","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Antiviral Effect of Microalgae Phaeodactylum tricornutum Protein Hydrolysates against Dengue Virus Serotype 2"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8210-0467","authenticated-orcid":false,"given":"Bianca Vianey","family":"Rivera-Serrano","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Autonomous University of Sinaloa, Culiac\u00e1n 80246, Mexico"},{"name":"Programa de Maestr\u00eda en Ciencias en Biomedicina Molecular, Facultad de Medicina, Universidad Aut\u00f3noma de Sinaloa (UAS), Culiac\u00e1n 80246, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandy Lucero","family":"Cabanillas-Salcido","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Autonomous University of Sinaloa, Culiac\u00e1n 80246, Mexico"},{"name":"Programa de Maestr\u00eda en Ciencias en Biomedicina Molecular, Facultad de Medicina, Universidad Aut\u00f3noma de Sinaloa (UAS), Culiac\u00e1n 80246, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5052-2670","authenticated-orcid":false,"given":"Carlos Daniel","family":"Cordero-Rivera","sequence":"additional","affiliation":[{"name":"Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2729-6210","authenticated-orcid":false,"given":"Ricardo","family":"Jim\u00e9nez-Camacho","sequence":"additional","affiliation":[{"name":"Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia Desiree","family":"Norzagaray-Valenzuela","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Autonomous University of Sinaloa, Culiac\u00e1n 80019, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loranda","family":"Calder\u00f3n-Zamora","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Autonomous University of Sinaloa, Culiac\u00e1n 80019, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1415-6260","authenticated-orcid":false,"given":"Luis Adri\u00e1n","family":"De Jes\u00fas-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n Biom\u00e9dica de Zacatecas, Instituto Mexicano del Seguro Social (IMSS), Zacatecas 98000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2379-8591","authenticated-orcid":false,"given":"Jos\u00e9 Manuel","family":"Reyes-Ruiz","sequence":"additional","affiliation":[{"name":"Unidad M\u00e9dica de Alta Especialidad, Hospital de Especialidades No. 14, Centro M\u00e9dico Nacional \u201cAdolfo Ruiz Cortines\u201d, Instituto Mexicano del Seguro Social (IMSS), Veracruz 91897, Mexico"},{"name":"Facultad de Medicina, Regi\u00f3n Veracruz, Universidad Veracruzana, Veracruz 91700, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Noe","family":"Farfan-Morales","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Naturales, Universidad Aut\u00f3noma Metropolitana (UAM), Unidad Cuajimalpa, Ciudad de M\u00e9xico 05348, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"Romero-Utrilla","sequence":"additional","affiliation":[{"name":"Departamento de Anatom\u00eda Patol\u00f3gica, Instituto Mexicano del Seguro Social (IMSS), Culiac\u00e1n 80200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edctor Manuel","family":"Ru\u00edz-Ruelas","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Autonomous University of Sinaloa, Culiac\u00e1n 80246, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josu\u00e9","family":"Camberos-Barraza","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Autonomous University of Sinaloa, Culiac\u00e1n 80246, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Camacho-Zamora","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Autonomous University of Sinaloa, Culiac\u00e1n 80246, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2307-0648","authenticated-orcid":false,"given":"Alberto Kousuke","family":"De la Herr\u00e1n-Arita","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Autonomous University of Sinaloa, Culiac\u00e1n 80246, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Angulo-Rojo","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Autonomous University of Sinaloa, Culiac\u00e1n 80246, Mexico"},{"name":"Departamento de Anatom\u00eda Patol\u00f3gica, Instituto Mexicano del Seguro Social (IMSS), Culiac\u00e1n 80200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4782-6398","authenticated-orcid":false,"given":"Alma Marlene","family":"Guadr\u00f3n-Llanos","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Autonomous University of Sinaloa, Culiac\u00e1n 80246, Mexico"},{"name":"Programa de Maestr\u00eda en Ciencias en Medicina Traslacional y Salud Publica, Facultad de Medicina, Universidad Aut\u00f3noma de Sinaloa (UAS), Culiac\u00e1n 80246, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1ngel Radam\u00e9s","family":"R\u00e1bago-Monz\u00f3n","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Autonomous University of Sinaloa, Culiac\u00e1n 80246, Mexico"},{"name":"Programa de Doctorado en Ciencias en Biomedicina Molecular, Facultad de Medicina, Universidad Aut\u00f3noma de Sinaloa (UAS), Culiac\u00e1n 80246, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6358-7839","authenticated-orcid":false,"given":"Janitzio Xiomara Korina","family":"Perales-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Bioprocesses Laboratory, Faculty of Chemical-Biological Sciences, Autonomous University of Sinaloa, Culiac\u00e1n 80246, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Antonio","family":"Valdez-Flores","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Autonomous University of Sinaloa, Culiac\u00e1n 80246, Mexico"},{"name":"Programa de Maestr\u00eda en Ciencias en Biomedicina Molecular, Facultad de Medicina, Universidad Aut\u00f3noma de Sinaloa (UAS), Culiac\u00e1n 80246, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6785-2035","authenticated-orcid":false,"given":"Rosa Mar\u00eda","family":"Del \u00c1ngel","sequence":"additional","affiliation":[{"name":"Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8280-9812","authenticated-orcid":false,"given":"Juan Fidel","family":"Osuna-Ramos","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Autonomous University of Sinaloa, Culiac\u00e1n 80246, Mexico"},{"name":"Programa de Maestr\u00eda en Ciencias en Biomedicina Molecular, Facultad de Medicina, Universidad Aut\u00f3noma de Sinaloa (UAS), Culiac\u00e1n 80246, Mexico"},{"name":"Departamento de Anatom\u00eda Patol\u00f3gica, Instituto Mexicano del Seguro Social (IMSS), Culiac\u00e1n 80200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/S0140-6736(23)02576-X","article-title":"Dengue","volume":"403","author":"Adams","year":"2024","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.1016\/j.vaccine.2020.04.011","article-title":"Arboviruses: A Global Public Health Threat","volume":"38","author":"Girard","year":"2020","journal-title":"Vaccine"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"taab146","DOI":"10.1093\/jtm\/taab146","article-title":"Global Burden for Dengue and the Evolving Pattern in the Past 30 Years","volume":"28","author":"Yang","year":"2021","journal-title":"J. Travel Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2296","DOI":"10.52711\/0974-360X.2021.00406","article-title":"A Review on Epidemiology of Dengue Viral Infection as an Emerging Disease","volume":"14","author":"Dehghani","year":"2021","journal-title":"Res. J. Pharm. Technol."},{"key":"ref_5","first-page":"1","article-title":"Epidemiological Surveillance of Dengue Fever: An Overview","volume":"5","author":"Kathiriya","year":"2020","journal-title":"Int. J. Vet. Sci. Amin. Hasb."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.jiph.2019.07.012","article-title":"Diagnosis of Severe Dengue: Challenges, Needs and Opportunities","volume":"13","author":"Wong","year":"2020","journal-title":"J. Infect. Public Health"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Carbone, D.A., Pellone, P., Lubritto, C., and Ciniglia, C. (2021). Evaluation of Microalgae Antiviral Activity and Their Bioactive Compounds. Antibiotics, 10.","DOI":"10.3390\/antibiotics10060746"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.crbiot.2021.04.003","article-title":"The Antiviral Potential of Algal-Derived Macromolecules","volume":"3","author":"Liu","year":"2021","journal-title":"Curr. Res. Biotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"S43","DOI":"10.18006\/2021.9(Spl-1-GCSGD_2020).S43.S48","article-title":"Antiviral Properties of Microalgae and Cyanobacteria","volume":"9","author":"Mahendran","year":"2021","journal-title":"J. Exp. Biol. Agric. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1021\/np060303s","article-title":"Cyclic Depsipeptides, Ichthyopeptins A and B, from Microcystis Ichthyoblabe","volume":"70","author":"Zainuddin","year":"2007","journal-title":"J. Nat. Prod."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s41240-016-0039-3","article-title":"A Spirulina Maxima-Derived Peptide Inhibits HIV-1 Infection in a Human T Cell Line MT4","volume":"19","author":"Jang","year":"2016","journal-title":"Fish. Aquat. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"103479","DOI":"10.1016\/j.algal.2024.103479","article-title":"Arthrospira Maxima Extract Prevents and Cures Zika Virus Infection: In vitro Analysis with VERO Cells","volume":"79","author":"Barros","year":"2024","journal-title":"Algal Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2871","DOI":"10.3390\/md8122871","article-title":"Potential Anti-HIV Agents from Marine Resources: An Overview","volume":"8","author":"Vo","year":"2010","journal-title":"Mar. Drugs"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Zhao, H., Zhou, J., Zhang, K., Chu, H., Liu, D., Poon, V.K.M., Chan, C., Leung, H., Fai, N., and Lin, Y. (2016). A Novel Peptide with Potent and Broad-Spectrum Antiviral Activities against Multiple Respiratory Viruses. Sci. Rep., 6.","DOI":"10.1038\/srep22008"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Rojas, V., Rivas, L., C\u00e1rdenas, C., and Guzm\u00e1n, F. (2020). Cyanobacteria and Eukaryotic Microalgae as Emerging Sources of Antibacterial Peptides. Molecules, 25.","DOI":"10.3390\/molecules25245804"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Rosales-Mendoza, S., Garc\u00eda-Silva, I., Gonz\u00e1lez-Ortega, O., Sandoval-Vargas, J.M., Malla, A., and Vimolmangkang, S. (2020). The Potential of Algal Biotechnology to Produce Antiviral Compounds and Biopharmaceuticals. Molecules, 25.","DOI":"10.3390\/molecules25184049"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4757","DOI":"10.1007\/s11033-021-06422-w","article-title":"Microalgae: Therapeutic Potentials and Applications","volume":"48","author":"Khavari","year":"2021","journal-title":"Mol. Biol. Rep."},{"key":"ref_18","first-page":"353","article-title":"Synthetic Peptides as Promising Antiviral Molecules","volume":"16","author":"Souza","year":"2021","journal-title":"Future Virol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ashour, M., and Omran, A.M.M. (2022). Recent Advances in Marine Microalgae Production: Highlighting Human Health Products from Microalgae in View of the Coronavirus Pandemic (COVID-19). Fermentation, 8.","DOI":"10.3390\/fermentation8090466"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"111338","DOI":"10.1016\/j.foodres.2022.111338","article-title":"Microalgae as a Sustainable Source of Edible Proteins and Bioactive Peptides\u2014Current Trends and Future Prospects","volume":"157","author":"Kumar","year":"2022","journal-title":"Food Res. Int."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1177\/1082013217717611","article-title":"Chemical Composition and Physicochemical Properties of Phaeodactylum tricornutum Microalgal Residual Biomass","volume":"23","author":"Shelton","year":"2017","journal-title":"Food Sci. Technol. Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.7150\/ijms.21875","article-title":"Peptides as Therapeutic Agents for Dengue Virus","volume":"14","author":"Chew","year":"2017","journal-title":"Int. J. Med. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1111\/cbdd.13400","article-title":"Novel Bioactive Peptides Demonstrating Anti-dengue Virus Activity Isolated from the Asian Medicinal Plant Acacia Catechu","volume":"93","author":"Panya","year":"2019","journal-title":"Chem. Biol. Drug Des."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.ijbiomac.2012.05.028","article-title":"Antiviral Activity against Dengue Virus of Diverse Classes of Algal Sulfated Polysaccharides","volume":"51","author":"Pujol","year":"2012","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1002\/cplu.202100351","article-title":"Antiviral Peptides with in vivo Activity: Development and Modes of Action","volume":"86","author":"Gao","year":"2021","journal-title":"Chempluschem"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.virol.2023.01.016","article-title":"Development of Novel Antiviral Peptides against Dengue Serotypes 1\u20134","volume":"580","author":"Lee","year":"2023","journal-title":"Virology"},{"key":"ref_27","first-page":"1188","article-title":"Molecular Docking, Simulations of Animal Peptides against the Envelope Protein of Dengue Virus","volume":"41","author":"Guntamadugu","year":"2023","journal-title":"J. Biomol. Struct. Dyn."},{"key":"ref_28","first-page":"160","article-title":"PD1 CD44 Antiviral Peptide as an Inhibitor of the Protein-Protein Interaction in Dengue Virus Invasion","volume":"38","author":"Giraldo","year":"2022","journal-title":"Peptides"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e01682-20","DOI":"10.1128\/JVI.01682-20","article-title":"Broad-Spectrum Antiviral Entry Inhibition by Interfacially Active Peptides","volume":"94","author":"Hoffmann","year":"2020","journal-title":"J. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"719","DOI":"10.7150\/ijms.5037","article-title":"Inhibition of Dengue Virus Entry into Target Cells Using Synthetic Antiviral Peptides","volume":"10","author":"Alhoot","year":"2013","journal-title":"Int. J. Med. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"739395","DOI":"10.1016\/j.aquaculture.2023.739395","article-title":"Mechanical Processing of Phaeodactylum tricornutum and Tetraselmis chui Biomass Affects Phenolic and Antioxidant Compound Availability, Nutrient Digestibility and Deposition of Carotenoids in Atlantic Salmon","volume":"569","author":"Kousoulaki","year":"2023","journal-title":"Aquaculture"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.3168\/jds.S0022-0302(73)85322-6","article-title":"Free Amino Acids and Soluble Peptides of Whey Powders","volume":"56","author":"Mavropoulou","year":"1973","journal-title":"J. Dairy Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"736783","DOI":"10.1016\/j.aquaculture.2021.736783","article-title":"Antiviral Peptides from Aquatic Organisms: Functionality and Potential Inhibitory Effect on SARS-CoV-2","volume":"541","author":"Sumon","year":"2021","journal-title":"Aquaculture"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.antiviral.2004.08.003","article-title":"A Wide Range of Medium-Sized, Highly Cationic, $\u03b1$-Helical Peptides Show Antiviral Activity against Herpes Simplex Virus","volume":"64","author":"Jenssen","year":"2004","journal-title":"Antiviral Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3963","DOI":"10.1016\/j.bmc.2019.07.038","article-title":"Peptide Derivatives as Inhibitors of NS2B-NS3 Protease from Dengue, West Nile, and Zika Flaviviruses","volume":"27","year":"2019","journal-title":"Bioorg. Med. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ji, M., Zhu, T., Xing, M., Luan, N., Mwangi, J., Yan, X., Mo, G., Rong, M., Li, B., and Lai, R. (2019). An Antiviral Peptide from Alopecosa Nagpag Spider Targets NS2B--NS3 Protease of Flaviviruses. Toxins, 11.","DOI":"10.3390\/toxins11100584"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Bharati, M., and Saha, D. (2018). Multiple Insecticide Resistance Mechanisms in Primary Dengue Vector, Aedes Aegypti (Linn.) from Dengue Endemic Districts of Sub-Himalayan West Bengal, India. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0203207"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"8151","DOI":"10.3390\/molecules19068151","article-title":"Natural Products as Source of Potential Dengue Antivirals","volume":"19","author":"Teixeira","year":"2014","journal-title":"Molecules"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1139\/m62-029","article-title":"Studies of Marine Planktonic Diatoms. I. Cyclotella Nana Hustedt and Detonula Confervacea Cleve","volume":"8","author":"Guilard","year":"1962","journal-title":"Gran Can J Microbiol"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s10811-016-0938-9","article-title":"Residual Biomasses and Protein Hydrolysates of Three Green Microalgae Species Exhibit Antioxidant and Anti-Aging Activity","volume":"29","author":"Shelton","year":"2017","journal-title":"J. Appl. Phycol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A Rapid Method of Total Lipid Extraction and Purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.algal.2013.12.004","article-title":"Aqueous Extraction of Proteins from Microalgae: Effect of Different Cell Disruption Methods","volume":"3","author":"Safi","year":"2014","journal-title":"Algal Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.jgeb.2018.01.002","article-title":"Scenedesmus Obliquus: Antioxidant and Antiviral Activity of Proteins Hydrolyzed by Three Enzymes","volume":"16","author":"Afify","year":"2018","journal-title":"J. Genet. Eng. Biotechnol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Hou, C., Wu, L., Wang, Z., Saguer, E., and Zhang, D. (2019). Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins. Antioxidants, 8.","DOI":"10.3390\/antiox8120592"}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/22\/8\/369\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:36:17Z","timestamp":1760110577000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/22\/8\/369"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,14]]},"references-count":44,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["md22080369"],"URL":"https:\/\/doi.org\/10.3390\/md22080369","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,14]]}}}