{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T06:52:58Z","timestamp":1781333578203,"version":"3.54.1"},"reference-count":51,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Marine Biodiversity Institute of Korea","award":["MABIK 2024M00600"],"award-info":[{"award-number":["MABIK 2024M00600"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>This study explores the biocontrol potential of Pediococcus sp. M21F004, a lactic acid bacteria (LAB) isolated from marine environments, against several bacterial and fungal phytopathogens. Out of 50 marine bacterial isolates, Pediococcus sp. M21F004 was selected for its exceptional antimicrobial activity. The strain, isolated from the intestine of a starry flounder, was identified as Pediococcus sp. Gas chromatography\u2013mass spectrometry (GC-MS) analysis revealed that oleic acid (OA) is a key antimicrobial compound produced by Pediococcus sp. M21F004. In vitro assays showed that the culture broth (CB) of Pediococcus sp. M21F004, as well as OA, exhibited significant inhibitory effects against pathogens such as Fusarium oxysporum, Clarireedia homoeocarpa, and Pectobacterium carotovorum subsp. carotovorum. In vivo tests on cucumber Fusarium wilt, creeping bentgrass dollar spot, tomato bacterial wilt, and kimchi cabbage soft rot further demonstrated the strain\u2019s efficacy in reducing disease severity. Moreover, OA had the highest control value of 74% against tomato bacterial wilt, followed by 64.1% against cucumber fusarium wilt, 42.5% against kimchi cabbage soft rot, and 16.5% against creeping bentgrass dollar spot. These findings suggest that Pediococcus sp. M21F004 and its metabolite OA offer promising alternatives to chemical pesticides, contributing to sustainable plant disease management by promoting resistance induction and providing an eco-friendly approach to agriculture.<\/jats:p>","DOI":"10.3390\/md22120534","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T07:28:01Z","timestamp":1732778881000},"page":"534","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Exploring Pediococcus sp. M21F004 for Biocontrol of Bacterial and Fungal Phytopathogens"],"prefix":"10.3390","volume":"22","author":[{"given":"Van Thi","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9989-6190","authenticated-orcid":false,"given":"Yong Min","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Biological Application and Technology, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4225-1812","authenticated-orcid":false,"given":"Ae Ran","family":"Park","sequence":"additional","affiliation":[{"name":"Plant Healthcare Research Institute, JAN153 Biotech Incorporated, Jeongeup 56212, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan Hee","family":"Yu","sequence":"additional","affiliation":[{"name":"Plant Healthcare Research Institute, JAN153 Biotech Incorporated, Jeongeup 56212, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8009-8006","authenticated-orcid":false,"given":"Grace","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Biomaterial Research, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0753-1966","authenticated-orcid":false,"given":"Jin-Cheol","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea"},{"name":"Plant Healthcare Research Institute, JAN153 Biotech Incorporated, Jeongeup 56212, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1038\/s41579-023-00900-7","article-title":"Climate change impacts on plant pathogens, food security and paths forward","volume":"21","author":"Singh","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Lindsey, A.P.J., Murugan, S., and Renitta, R.E. (2020). Microbial disease management in agriculture: Current status and future prospects. Biocatal. Agric. Biotechnol., 23.","DOI":"10.1016\/j.bcab.2019.101468"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Shahid, M., and Khan, M.S. (2022). Ecotoxicological implications of residual pesticides to beneficial soil bacteria: A review. Pestic. Biochem. Physiol., 188.","DOI":"10.1016\/j.pestbp.2022.105272"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"103356","DOI":"10.1016\/j.apsoil.2019.09.006","article-title":"Soil biological activity as an indicator of soil pollution with pesticides\u2013a review","volume":"147","author":"Wydro","year":"2020","journal-title":"Appl. Soil. Ecol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Nguyen, V.T., Yu, N.H., Lee, Y., Hwang, I.M., Bui, H.X., and Kim, J.-C. (2021). Nematicidal activity of cyclopiazonic acid derived from Penicillium commune against root-knot nematodes and optimization of the culture fermentation process. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.726504"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Le, K.D., Kim, J., Nguyen, H.T., Yu, N.H., Park, A.R., Lee, C.W., and Kim, J.-C. (2021). Streptomyces sp. JCK-6131 protects plants against bacterial and fungal diseases via two mechanisms. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.726266"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Cui, W., Zhang, J., Wang, W., Wu, X., Luo, X., Zou, Y., Chen, K., and He, P. (2024). Screening native Bacillus strains as potential biological control agents against ginger bacterial wilt and for promoting plant growth. Biol. Control, 192.","DOI":"10.1016\/j.biocontrol.2024.105510"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Benaissa, A. (2024). Rhizosphere: Role of bacteria to manage plant diseases and sustainable agriculture\u2014A review. J. Basic Microbiol., 64.","DOI":"10.1002\/jobm.202300361"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Hirozawa, M.T., Ono, M.A., Suguiura, I.M.S., Bordini, J.G., and Ono, E.Y.S. (2023). Lactic acid bacteria and Bacillus spp. as fungal biological control agents. J. Appl. Microbiol., 134.","DOI":"10.1093\/jambio\/lxac083"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1007\/s10340-022-01573-6","article-title":"Control of root-knot nematodes on tomato by eliciting resistance through Aspergillus niger-derived oxalic acid","volume":"96","author":"Yeon","year":"2023","journal-title":"J. Pest. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1264\/jsme2.ME14144","article-title":"Recent trends in control methods for bacterial wilt diseases caused by Ralstonia solanacearum","volume":"30","author":"Nion","year":"2015","journal-title":"Microbes Environ."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Poli, A., Finore, I., Romano, I., Gioiello, A., Lama, L., and Nicolaus, B. (2017). Microbial diversity in extreme marine habitats and their biomolecules. Microorganisms, 5.","DOI":"10.3390\/microorganisms5020025"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1111\/jam.12041","article-title":"Screening and characterization of LAB-produced bacteriocin-like substances from the intestine of grey mullet (Mugil cephalus L.) as potential biocontrol agents in aquaculture","volume":"114","author":"Lin","year":"2013","journal-title":"J. Appl. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1128\/MMBR.64.4.655-671.2000","article-title":"Probiotic bacteria as biological control agents in aquaculture","volume":"64","author":"Verschuere","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1007\/s10499-016-0045-6","article-title":"In vitro screening of lactic acid bacteria isolated from gastrointestinal tract of Atlantic Salmon (Salmo salar) as probiont candidates","volume":"25","author":"Amin","year":"2017","journal-title":"Aquacul. Int."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Mokoena, M.P. (2017). Lactic acid bacteria and their bacteriocins: Classification, biosynthesis and applications against uropathogens: A mini-review. Molecules, 22.","DOI":"10.3390\/molecules22081255"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1128\/MMBR.00076-15","article-title":"Stress physiology of lactic acid bacteria","volume":"80","author":"Papadimitriou","year":"2016","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.4315\/0362-028X-73.6.1129","article-title":"Potential of Pediococcus pentosaceus (L006) isolated from maize leaf to suppress fumonisin-producing fungal growth","volume":"73","author":"Dalie","year":"2010","journal-title":"J. Food Prot."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Sellamani, M., Kalagatur, N.K., Siddaiah, C., Mudili, V., Krishna, K., Natarajan, G., and Rao Putcha, V.L. (2016). Antifungal and zearalenone inhibitory activity of Pediococcus pentosaceus isolated from dairy products on Fusarium graminearum. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.00890"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Riolo, M., Luz, C., Santilli, E., Meca, G., and Cacciola, S.O. (2023). Antifungal activity of selected lactic acid bacteria from olive drupes. Food Biosci., 52.","DOI":"10.1016\/j.fbio.2023.102422"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1111\/jam.15205","article-title":"Antimicrobial properties of Pediococcus acidilactici and Pediococcus pentosaceus isolated from silage","volume":"132","author":"Fugaban","year":"2022","journal-title":"J. Appl. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Huynh, U., and Zastrow, M.L. (2023). Metallobiology of Lactobacillaceae in the gut microbiome. J. Inorg. Biochem., 238.","DOI":"10.1016\/j.jinorgbio.2022.112023"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Rossi, F., Amadoro, C., Gasperi, M., and Colavita, G. (2022). Lactobacilli infection case reports in the last three years and safety implications. Nutrients, 14.","DOI":"10.20944\/preprints202202.0248.v1"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA) (2021). EFSA statement on the requirements for whole genome sequence analysis of microorganisms intentionally used in the food chain. EFSA J., 19, e06506.","DOI":"10.2903\/j.efsa.2021.6506"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1111\/j.1365-2672.2007.03619.x","article-title":"Lactic acid bacteria with potential to eliminate fungal spoilage in foods","volume":"104","author":"Rouse","year":"2008","journal-title":"J. Appl. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2622","DOI":"10.1111\/j.1365-2672.2007.03525.x","article-title":"Antifungal lactic acid bacteria with potential to prolong shelf-life of fresh vegetables","volume":"103","author":"Sathe","year":"2007","journal-title":"J. Appl. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Gao, J., Lin, G., Deng, X., Zou, J., Liu, Y., Chen, X., and Liu, S. (2024). Development of tetramycin-loaded core\u2013shell beads with hot-\/wet-responsive release properties for control of bacterial wilt disease. Agronomy, 14.","DOI":"10.20944\/preprints202402.0841.v1"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ghavam, M., Afzali, A., and Manca, M.L. (2021). Chemotype of damask rose with oleic acid (9 octadecenoic acid) and its antimicrobial effectiveness. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-87604-1"},{"key":"ref_29","first-page":"217","article-title":"Effects of sesame seed cake allelochemicals on the growth cucumber (Cucumis sativus L. cv. Jinchun 4)","volume":"16","author":"Ruan","year":"2005","journal-title":"Allelopath. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/S0367-326X(00)00150-7","article-title":"Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatum: A plant used during circumcision rites","volume":"71","author":"Dilika","year":"2000","journal-title":"Fitoterapia"},{"key":"ref_31","first-page":"775","article-title":"Antibacterial and antifungal efficacy of fatty acid methyl esters from the leaves of Sesuvium portulacastrum L.","volume":"15","author":"Chandrasekaran","year":"2011","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.plipres.2007.10.002","article-title":"New advances in fatty acids as antimalarial, antimycobacterial and antifungal agents","volume":"47","author":"Carballeira","year":"2008","journal-title":"Prog. Lipid Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1128\/AAC.2.1.23","article-title":"Fatty acids and derivatives as antimicrobial agents","volume":"2","author":"Kabara","year":"1972","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4899","DOI":"10.1039\/D0TB00106F","article-title":"Encapsulating insoluble antifungal drugs into oleic acid-modified silica mesocomposites with enhanced fungicidal activity","volume":"8","author":"Zhu","year":"2020","journal-title":"J. Mater. Chem. B"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Ramadan, A.M.A.A., Zidan, S.A.H., Shehata, R.M., El-Sheikh, H.H., Ameen, F., Stephenson, S.L., and Al-Bedak, O.A.-H.M. (2024). Antioxidant, antibacterial, and molecular docking of methyl ferulate and oleic acid produced by Aspergillus pseudodeflectus AUMC 15761 utilizing wheat bran. Sci. Rep., 14.","DOI":"10.1038\/s41598-024-58336-9"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1023\/B:MYCO.0000012222.68156.2c","article-title":"Antifungal activities of four fatty acids against plant pathogenic fungi","volume":"157","author":"Walters","year":"2004","journal-title":"Mycopathologia"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1002\/ps.7846","article-title":"Antagonistic activity of Trichoderma asperellum against Fusarium species, chemical profile and their efficacy for management of Fusarium-root rot disease in dry bean","volume":"80","author":"Elshahawy","year":"2024","journal-title":"Pest Manag. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s11046-008-9124-1","article-title":"Biological control of phytopathogenic fungi by fatty acids","volume":"166","author":"Liu","year":"2008","journal-title":"Mycopathologia"},{"key":"ref_39","unstructured":"Stackebrandt, E., and Goodfellow, M. (1991). 16S\/23S rRNA sequencing. Nucleic Acid Techniques in Bacterial Systematic, John Wiley and Sons."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1099\/ijsem.0.000760","article-title":"OrthoANI: An improved algorithm and software for calculating average nucleotide identity","volume":"66","author":"Lee","year":"2016","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_41","first-page":"406","article-title":"The neighbor-joining method: A new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1007\/BF01734359","article-title":"Evolutionary trees from DNA sequences: A maximum likelihood approach","volume":"17","author":"Felsenstein","year":"1981","journal-title":"J. Mol. Evol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1093\/sysbio\/20.4.406","article-title":"Toward defining the course of evolution: Minimum change for a specific tree topology","volume":"20","author":"Fitch","year":"1971","journal-title":"Syst. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"Mega X: Molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Nawrocki, E.P., and Eddy, S.R. (2007). Query-dependent banding (QDB) for faster RNA similarity searches. PLoS Comput. Biol., 3.","DOI":"10.1371\/journal.pcbi.0030056"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13007-021-00720-x","article-title":"Optimization of protoplast regeneration in the model plant Arabidopsis thaliana","volume":"17","author":"Jeong","year":"2021","journal-title":"Plant Methods"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Yeo, Y.J., Park, A.R., Vuong, B.S., and Kim, J.-C. (2024). Biocontrol of Fusarium head blight in rice using Bacillus velezensis JCK-7158. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1358689"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"245","DOI":"10.5423\/RPD.2014.20.4.245","article-title":"Efficient screening method for resistance of cucumber cultivars to Fusarium oxysporum f. sp. cucumerinum","volume":"20","author":"Lee","year":"2014","journal-title":"Res. Plant Dis."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"598","DOI":"10.5423\/PPJ.OA.05.2019.0129","article-title":"Biocontrol potential of fungal endophytes against Fusarium oxysporum f. sp. cucumerinum causing wilt in cucumber","volume":"35","author":"Abro","year":"2019","journal-title":"Plant Pathol. J."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Le, K.D., Kim, J., Yu, N.H., Kim, B., Lee, C.W., and Kim, J.-C. (2020). Biological control of tomato bacterial wilt, kimchi cabbage soft rot, and red pepper bacterial leaf spot using Paenibacillus elgii JCK-5075. Front. Plant Sci., 11.","DOI":"10.3389\/fpls.2020.00775"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1094\/PHYTO-96-0033","article-title":"Variation in albicidin biosynthesis genes and in pathogenicity of Xanthomonas albilineans, the sugarcane leaf scald pathogen","volume":"96","author":"Champoiseau","year":"2006","journal-title":"Phytopathology"}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/22\/12\/534\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:41:29Z","timestamp":1760114489000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/22\/12\/534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,28]]},"references-count":51,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["md22120534"],"URL":"https:\/\/doi.org\/10.3390\/md22120534","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,28]]}}}