{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T09:00:26Z","timestamp":1787994026030,"version":"build-2784847793"},"reference-count":74,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T00:00:00Z","timestamp":1737504000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EU Interreg: Northwest Europe (NWE)","award":["NWE639"],"award-info":[{"award-number":["NWE639"]}]},{"name":"EU Interreg: Northwest Europe (NWE)","award":["E63C22000990007"],"award-info":[{"award-number":["E63C22000990007"]}]},{"name":"European Union\u2014NextGenerationEU \u201cCentro Nazionale Biodiversit\u00e0 CN5\u201d\u2014CUP","award":["NWE639"],"award-info":[{"award-number":["NWE639"]}]},{"name":"European Union\u2014NextGenerationEU \u201cCentro Nazionale Biodiversit\u00e0 CN5\u201d\u2014CUP","award":["E63C22000990007"],"award-info":[{"award-number":["E63C22000990007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>BIOPEP-UWM, a peptide database, contains 5128 peptides from a myriad of resources. Five listed peptides are Angiotensin-I-converting enzyme (ACE-1; EC3.4.15.1) inhibitory peptides derived from a red alga, while two from Chlorella vulgaris have anti-cancer and antioxidative bioactivities. Herein, we describe a process combining hydrolysis with two enzymes, Alcalase and Viscozyme, and filtration to generate protein-rich, bioactive peptide-containing hydrolysates from mixed species of Chlorella sp. and Scenedesmus sp. The potential of generated algal hydrolysates to act as food ingredients was determined by assessment of their techno-functional (foaming, emulsification, solubility, water holding, and oil holding capacity) properties. Bioactive screening of hydrolysates in vitro combined with mass spectrometry (MS) and in silico predictions identified bioactive and functional hydrolysates and six novel peptides. Peptides derived from Chlorella mix have the sequences YDYIGNNPAKGGLF and YIGNNPAKGGLF with predicted anti-inflammatory (medium confidence) and umami potential. Peptides from Scenedesmus mix have sequences IEWYGPDRPKFL, RSPTGEIIFGGETM, TVQIPGGERVPFLF, and IEWYGPDRPKFLGPF with predicted anti-inflammatory, anti-diabetic, and umami attributes. Such microalgal hydrolysates could provide essential amino acids to consumers as well as tertiary health benefits to improve human global health.<\/jats:p>","DOI":"10.3390\/md23020053","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T09:52:20Z","timestamp":1737539540000},"page":"53","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Functional and Bioactive Benefits of Selected Microalgal Hydrolysates Assessed In Silico and In Vitro"],"prefix":"10.3390","volume":"23","author":[{"given":"Elena","family":"Aurino","sequence":"first","affiliation":[{"name":"Dipartimento di Ingegneria Chimica, dei Materiali, e della Produzione Industriale, Universit\u00e0 degli Studi di Napoli \u2018Federico II\u2019, Piazzale Tecchio, 80, 80125 Naples, Italy"},{"name":"Food BioSciences, Teagasc Food Research Centre, Ashtown, D15 DY05 Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1571-5781","authenticated-orcid":false,"given":"Leticia","family":"Mora","sequence":"additional","affiliation":[{"name":"Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (CSIC), Avenue Agust\u00edn Escardino 7, 46980 Paterna, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8124-9419","authenticated-orcid":false,"given":"Antonio","family":"Marzocchella","sequence":"additional","affiliation":[{"name":"Dipartimento di Ingegneria Chimica, dei Materiali, e della Produzione Industriale, Universit\u00e0 degli Studi di Napoli \u2018Federico II\u2019, Piazzale Tecchio, 80, 80125 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4383-5775","authenticated-orcid":false,"given":"Christina M.","family":"Kuchendorf","sequence":"additional","affiliation":[{"name":"Institute of Bio- and Geosciences, IBG-2\u2014Plant Sciences Forschungszentrum J\u00fclich GmbH, Wilhelm-Johnen-Str., 52428 J\u00fclich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e4rbel","family":"Ackermann","sequence":"additional","affiliation":[{"name":"Stadt Erftstadt, Stabsstelle Klimaschutz, Holzdamm 10, 50374 Erftstadt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Hayes","sequence":"additional","affiliation":[{"name":"Food BioSciences, Teagasc Food Research Centre, Ashtown, D15 DY05 Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1007\/s00394-024-03358-2","article-title":"Meeting the global protein supply requirements of a growing and ageing population","volume":"63","author":"Smith","year":"2024","journal-title":"Eur. 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