{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:43:10Z","timestamp":1759970590767,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T00:00:00Z","timestamp":1737936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Science, Innovation and Universities","award":["PID2023-146042OB-C21","PID2020\u2013117009RB-I00","IN607B2023\/05","A1-S-9762 (863069)"],"award-info":[{"award-number":["PID2023-146042OB-C21","PID2020\u2013117009RB-I00","IN607B2023\/05","A1-S-9762 (863069)"]}]},{"name":"Xunta de Galicia\u2014Axencia Galega de Innovaci\u00f3n (GAIN)","award":["PID2023-146042OB-C21","PID2020\u2013117009RB-I00","IN607B2023\/05","A1-S-9762 (863069)"],"award-info":[{"award-number":["PID2023-146042OB-C21","PID2020\u2013117009RB-I00","IN607B2023\/05","A1-S-9762 (863069)"]}]},{"name":"CONAHCyT proyect","award":["PID2023-146042OB-C21","PID2020\u2013117009RB-I00","IN607B2023\/05","A1-S-9762 (863069)"],"award-info":[{"award-number":["PID2023-146042OB-C21","PID2020\u2013117009RB-I00","IN607B2023\/05","A1-S-9762 (863069)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Marine organisms are a vital source of biologically active compounds. Organic extracts from the muscle of the Pacific white shrimp (L. vannamei) have shown antiproliferative effects on tumor cells, including breast adenocarcinoma. This study aimed to analyze these extracts\u2019 composition and confirm their specificity for breast adenocarcinoma cells without harming normal cells. An organic chloroform extract from L. vannamei muscle was divided using a solvent partition procedure with methanol and hexane. The methanolic partition was fractionated through an open preparative liquid chromatography column to isolate compounds with biological activity, that were later tested on MDA-MB-231 (breast adenocarcinoma), and recently tested on MCF10-A (non-cancerous breast epithelial cells). Cells incubated with these fractions were assessed for viability and morphological changes using fluorescence confocal microscopy. Fractions F#13 and F#14 reduced MDA-MB-231 cancer cell viability at 100 \u00b5g\/mL without affecting non-cancerous MCF-10A cells, inducing apoptosis-related changes in cancer cells. These fractions contained EPA and DHA free fatty acids, specifically F#13 contained free and esterified astaxanthin as well. The high levels of free linoleic acid 18:2 \u03c9-6, EPA, and DHA (in a 2:1 ratio, EPA:DHA), along with free and esterified astaxanthin in F#13, significantly reduced breast adenocarcinoma cell viability, nearly to that achieved by cisplatin, a chemotherapy drug.<\/jats:p>","DOI":"10.3390\/md23020059","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T11:38:49Z","timestamp":1737977929000},"page":"59","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical Characterization of Bioactive Compounds in Extracts and Fractions from Litopenaeus vannamei Muscle"],"prefix":"10.3390","volume":"23","author":[{"given":"Sandra Carolina","family":"De La Re\u00e9-Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Departamento de Investigaci\u00f3n y Posgrado en Alimentos, Universidad de Sonora, Hermosillo 83000, Sonora, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2296-185X","authenticated-orcid":false,"given":"Mar\u00eda Jes\u00fas","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Qu\u00edmica de Productos Marinos, Instituto de Investigaciones Marinas, 36208 Vigo, Pontevedra, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Qu\u00edmica de Productos Marinos, Instituto de Investigaciones Marinas, 36208 Vigo, Pontevedra, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Luis","family":"Garrido","sequence":"additional","affiliation":[{"name":"Qu\u00edmica de Productos Marinos, Instituto de Investigaciones Marinas, 36208 Vigo, Pontevedra, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6019-858X","authenticated-orcid":false,"given":"Erika","family":"Silva-Campa","sequence":"additional","affiliation":[{"name":"Departamento de Investigaci\u00f3n en F\u00edsica, Universidad de Sonora, Hermosillo 83000, Sonora, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norma Violeta","family":"Parra-Vergara","sequence":"additional","affiliation":[{"name":"Departamento de Investigaci\u00f3n y Posgrado en Alimentos, Universidad de Sonora, Hermosillo 83000, Sonora, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen Mar\u00eda","family":"L\u00f3pez-Saiz","sequence":"additional","affiliation":[{"name":"Departamento de Investigaci\u00f3n y Posgrado en Alimentos, Universidad de Sonora, Hermosillo 83000, Sonora, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Medina","sequence":"additional","affiliation":[{"name":"Qu\u00edmica de Productos Marinos, Instituto de Investigaciones Marinas, 36208 Vigo, Pontevedra, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,27]]},"reference":[{"key":"ref_1","unstructured":"P\u00e9rez-Farfante, I., and Kensley, B. 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