{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T08:49:52Z","timestamp":1787820592376,"version":"build-2784847793"},"reference-count":34,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["2020GXNSFFA297005"],"award-info":[{"award-number":["2020GXNSFFA297005"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["32160220"],"award-info":[{"award-number":["32160220"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["82373788"],"award-info":[{"award-number":["82373788"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["AD21159002"],"award-info":[{"award-number":["AD21159002"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["202310600026"],"award-info":[{"award-number":["202310600026"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2020GXNSFFA297005"],"award-info":[{"award-number":["2020GXNSFFA297005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160220"],"award-info":[{"award-number":["32160220"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82373788"],"award-info":[{"award-number":["82373788"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["AD21159002"],"award-info":[{"award-number":["AD21159002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["202310600026"],"award-info":[{"award-number":["202310600026"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018571","name":"Specific Research Project of Guangxi for Research Bases and Talents","doi-asserted-by":"publisher","award":["2020GXNSFFA297005"],"award-info":[{"award-number":["2020GXNSFFA297005"]}],"id":[{"id":"10.13039\/501100018571","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018571","name":"Specific Research Project of Guangxi for Research Bases and Talents","doi-asserted-by":"publisher","award":["32160220"],"award-info":[{"award-number":["32160220"]}],"id":[{"id":"10.13039\/501100018571","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018571","name":"Specific Research Project of Guangxi for Research Bases and Talents","doi-asserted-by":"publisher","award":["82373788"],"award-info":[{"award-number":["82373788"]}],"id":[{"id":"10.13039\/501100018571","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018571","name":"Specific Research Project of Guangxi for Research Bases and Talents","doi-asserted-by":"publisher","award":["AD21159002"],"award-info":[{"award-number":["AD21159002"]}],"id":[{"id":"10.13039\/501100018571","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018571","name":"Specific Research Project of Guangxi for Research Bases and Talents","doi-asserted-by":"publisher","award":["202310600026"],"award-info":[{"award-number":["202310600026"]}],"id":[{"id":"10.13039\/501100018571","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangxi Bagui Youth Top Talent Project","award":["2020GXNSFFA297005"],"award-info":[{"award-number":["2020GXNSFFA297005"]}]},{"name":"Guangxi Bagui Youth Top Talent Project","award":["32160220"],"award-info":[{"award-number":["32160220"]}]},{"name":"Guangxi Bagui Youth Top Talent Project","award":["82373788"],"award-info":[{"award-number":["82373788"]}]},{"name":"Guangxi Bagui Youth Top Talent Project","award":["AD21159002"],"award-info":[{"award-number":["AD21159002"]}]},{"name":"Guangxi Bagui Youth Top Talent Project","award":["202310600026"],"award-info":[{"award-number":["202310600026"]}]},{"name":"Innovation and Entrepreneurship Training Program for College Students","award":["2020GXNSFFA297005"],"award-info":[{"award-number":["2020GXNSFFA297005"]}]},{"name":"Innovation and Entrepreneurship Training Program for College Students","award":["32160220"],"award-info":[{"award-number":["32160220"]}]},{"name":"Innovation and Entrepreneurship Training Program for College Students","award":["82373788"],"award-info":[{"award-number":["82373788"]}]},{"name":"Innovation and Entrepreneurship Training Program for College Students","award":["AD21159002"],"award-info":[{"award-number":["AD21159002"]}]},{"name":"Innovation and Entrepreneurship Training Program for College Students","award":["202310600026"],"award-info":[{"award-number":["202310600026"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The depolymerized products and oligosaccharide fractions from sea cucumber fucosylated glycosaminoglycans (FGs) are promising anticoagulant candidates, and more novel FG-derived oligosaccharides from low-priced sea cucumbers are expected to be obtained. This study isolated 5\u221212 oligomers (OF1\u2212OF3) with unusual branches from \u03b2-eliminative depolymerized products of Colochirus quadrangularis FG (CqFG). Detailed NMR analyses showed that OF1\u2212OF3 consisted of a chondroitin 4,6-sulfates backbone and some sulfated fucosyl branches (FucS), including monosaccharides (\u03b1-l-Fuc2S4S, \u03b1-l-Fuc3S, \u03b1-l-Fuc4S, \u03b1-l-Fuc2S3S4S, and \u03b1-l-Fuc2S) and a disaccharide D-Gal3S4S-\u03b11,3-l-Fuc2S4S with the ratio of ~36:35:10:7:3:9, attached to the C-3 position of \u03b2-d-GlcA or its derivatives, such as \u03b1-l-\u03944,5GlcA and \u03b2-d-GlcA-ol. Unusually, \u03b1-l-Fuc3S was the main FucS branch; no \u03b1-l-Fuc3S4S branch was found, and \u03b1-l-Fuc2S3S4S and \u03b1-l-Fuc2S branches were also found in OF1\u2013OF3. The OF2 and OF3 could strongly inhibit the intrinsic and common coagulation pathways. Intrinsic FXase is a target of OF2 and OF3 inhibiting the intrinsic coagulation pathways, and the unusual side chains may increase the intrinsic FXase inhibitory activity. OF2 and OF3 showed negligible bleeding risk, and less bleeding than heparin (HP), low-molecular-weight heparins (LMWHs), and CqFG. These findings support novel FG oligosaccharides with some unusual branches from low-priced sea cucumbers to be prepared as safer anticoagulants.<\/jats:p>","DOI":"10.3390\/md23020064","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T12:18:56Z","timestamp":1738585136000},"page":"64","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Structural Characterization and Anticoagulant Potential of Colochirus quadrangularis Fucosylated Glycosaminoglycan 5\u221212 Oligomers with Unusual Branches"],"prefix":"10.3390","volume":"23","author":[{"given":"Xuedong","family":"Zhang","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Marine Drugs, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangwei","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinming","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Drugs, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiewen","family":"Fu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Drugs, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Shi","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Drugs, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"},{"name":"College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2298-4667","authenticated-orcid":false,"given":"Pei","family":"Cao","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Drugs, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqian","family":"Sun","sequence":"additional","affiliation":[{"name":"Instrumentation and Service Center for Molecular Sciences, Westlake University, Hangzhou 310024, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengping","family":"Zhong","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Drugs, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiale","family":"Nong","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Drugs, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiqi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Drugs, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8327-3108","authenticated-orcid":false,"given":"Yonghong","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Drugs, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoshun","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8023-3944","authenticated-orcid":false,"given":"Qingxia","family":"Yuan","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Drugs, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Drugs, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1126\/science.1207478","article-title":"Chemoenzymatic Synthesis of Homogeneous Ultralow Molecular Weight Heparins","volume":"334","author":"Xu","year":"2011","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"eaan5954","DOI":"10.1126\/scitranslmed.aan5954","article-title":"Synthetic Oligosaccharides Can Replace Animal-Sourced Low\u2013Molecular Weight Heparins","volume":"9","author":"Xu","year":"2017","journal-title":"Sci. Transl. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2338","DOI":"10.1039\/C7CS00593H","article-title":"Design, Synthesis, and Biomedical Applications of Synthetic Sulphated Polysaccharides","volume":"48","author":"Caputo","year":"2019","journal-title":"Chem. Soc. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"120956","DOI":"10.1016\/j.carbpol.2023.120956","article-title":"A Regular Chlorella Mannogalactan and Its Sulfated Derivative as a Promising Anticoagulant: Structural Characterization and Anticoagulant Activity","volume":"314","author":"Tang","year":"2023","journal-title":"Carbohydr. Polym."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"100615","DOI":"10.1016\/j.blre.2019.100615","article-title":"From Multi-Target Anticoagulants to DOACs, and Intrinsic Coagulation Factor Inhibitors","volume":"39","author":"Lin","year":"2020","journal-title":"Blood Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"117034","DOI":"10.1016\/j.carbpol.2020.117034","article-title":"Low-Molecular-Weight Fucosylated Glycosaminoglycan and Its Oligosaccharides from Sea Cucumber as Novel Anticoagulants: A Review","volume":"251","author":"Li","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"232","DOI":"10.3390\/md12010232","article-title":"Holothurian Fucosylated Chondroitin Sulfate","volume":"12","author":"Pomin","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1016\/j.carbpol.2013.07.063","article-title":"Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan Degraded by Deaminative Cleavage","volume":"98","author":"Zhao","year":"2013","journal-title":"Carbohydr. Polym."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8284","DOI":"10.1073\/pnas.1504229112","article-title":"Discovery of an Intrinsic Tenase Complex Inhibitor: Pure Nonasaccharide from Fucosylated Glycosaminoglycan","volume":"112","author":"Zhao","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"14089","DOI":"10.1074\/jbc.RA118.003809","article-title":"Oligosaccharides from Depolymerized Fucosylated Glycosaminoglycan: Structures and Minimum Size for Intrinsic Factor Xase Complex Inhibition","volume":"293","author":"Yin","year":"2018","journal-title":"J. Biol. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"123145","DOI":"10.1016\/j.carbpol.2024.123145","article-title":"Oligosaccharide-Assisted Resolution of Holothurian Fucosylated Chondroitin Sulfate for Fine Structure and P-Selectin Inhibition","volume":"351","author":"Pan","year":"2025","journal-title":"Carbohydr. Polym."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1021\/acs.biomac.0c01739","article-title":"Unveiling the Disaccharide-Branched Glycosaminoglycan and Anticoagulant Potential of Its Derivatives","volume":"22","author":"Yin","year":"2021","journal-title":"Biomacromolecules"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"118290","DOI":"10.1016\/j.carbpol.2021.118290","article-title":"Structural Characterization and Anticoagulant Analysis of the Novel Branched Fucosylated Glycosaminoglycan from Sea Cucumber Holothuria Nobilis","volume":"269","author":"Li","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"118290","DOI":"10.1016\/j.carbpol.2020.116503","article-title":"A New Fucosylated Glycosaminoglycan Containing Disaccharide Branches from Acaudina Molpadioides: Unusual Structure and Anti-Intrinsic Tenase Activity","volume":"245","author":"Mao","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"120072","DOI":"10.1016\/j.carbpol.2022.120072","article-title":"Deaminative-Cleaved, S. Monotuberculatus Fucosylated Glycosaminoglycan: Structural Elucidation and Anticoagulant Activity","volume":"298","author":"Yuan","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"121304","DOI":"10.1016\/j.carbpol.2023.121304","article-title":"Branch Distribution Pattern and Anticoagulant Activity of a Fucosylated Chondroitin Sulfate from Phyllophorella Kohkutiensis","volume":"321","author":"Lan","year":"2023","journal-title":"Carbohydr. Polym."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"119826","DOI":"10.1016\/j.carbpol.2022.119826","article-title":"Re-Understanding of Structure and Anticoagulation: Fucosylated Chondroitin Sulfate from Sea Cucumber Ludwigothurea Grisea","volume":"294","author":"Yin","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1080\/23802359.2020.1748542","article-title":"The Complete Mitochondrial Genome of Colochirus Quadrangularis (Dendrochirotida, Cucumariidae)","volume":"5","author":"Zeng","year":"2020","journal-title":"Mitochondrial DNA Part. B"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3421","DOI":"10.1016\/j.ijbiomac.2020.08.150","article-title":"Purification, Structural Characterization and Anticoagulant Activities of Four Sulfated Polysaccharides from Sea Cucumber Holothuria Fuscopunctata","volume":"164","author":"Gao","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"116337","DOI":"10.1016\/j.carbpol.2020.116337","article-title":"Structure Characterization of a Heavily Fucosylated Chondroitin Sulfate from Sea Cucumber (H. Leucospilota) with Bottom-up Strategies","volume":"240","author":"Qiu","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1093\/glycob\/cwx031","article-title":"Exploring the Structure of Fucosylated Chondroitin Sulfate through Bottom-up Nuclear Magnetic Resonance and Electrospray Ionization-High-Resolution Mass Spectrometry Approaches","volume":"27","author":"Santos","year":"2017","journal-title":"Glycobiology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5081","DOI":"10.1016\/S0021-9258(19)34087-6","article-title":"Occurrence of Chondroitin Sulfate E in Glycosaminoglycan Isolated from the Body Wall of Sea Cucumber Stichopus Japonicus","volume":"265","author":"Kariya","year":"1990","journal-title":"J. Biol. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Guan, R., Peng, Y., Zhou, L., Zheng, W., Liu, X., Wang, P., Yuan, Q., Gao, N., Zhao, L., and Zhao, J. (2019). Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus Japonicus: Analysis of Its Depolymerized Fragments. Mar. Drugs, 17.","DOI":"10.3390\/md17040195"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"112796","DOI":"10.1016\/j.ejmech.2020.112796","article-title":"The Components and Activities Analysis of a Novel Anticoagulant Candidate DHG-5","volume":"207","author":"Sun","year":"2020","journal-title":"Eur. J. Med. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"119072","DOI":"10.1016\/j.carbpol.2021.119072","article-title":"Depolymerization of a Fucosylated Chondroitin Sulfate from Cucumaria Japonica: Structure and Activity of the Product","volume":"281","author":"Ustyuzhanina","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"115895","DOI":"10.1016\/j.carbpol.2020.115895","article-title":"Fucosylated Chondroitin Sulfate from the Sea Cucumber Hemioedema Spectabilis: Structure and Influence on Cell Adhesion and Tubulogenesis","volume":"234","author":"Ustyuzhanina","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1016\/j.carbpol.2017.10.100","article-title":"A Novel Structural Fucosylated Chondroitin Sulfate from Holothuria Mexicana and Its Effects on Growth Factors Binding and Anticoagulation","volume":"181","author":"Li","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.ejmech.2014.12.054","article-title":"Anticoagulant and Antithrombotic Evaluation of Native Fucosylated Chondroitin Sulfates and Their Derivatives as Selective Inhibitors of Intrinsic Factor Xase","volume":"92","author":"Wu","year":"2015","journal-title":"Eur. J. Med. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1016\/j.carbpol.2011.08.082","article-title":"Structure and Effect of Sulfated Fucose Branches on Anticoagulant Activity of the Fucosylated Chondroitin Sulfate from Sea Cucumber Thelenata Ananas","volume":"87","author":"Wu","year":"2012","journal-title":"Carbohydr. Polym."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1055\/s-0040-1708480","article-title":"Effects of Native Fucosylated Glycosaminoglycan, Its Depolymerized Derivatives on Intrinsic Factor Xase, Coagulation, Thrombosis, and Hemorrhagic Risk","volume":"120","author":"Zhou","year":"2020","journal-title":"Thromb. Haemost."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"115146","DOI":"10.1016\/j.carbpol.2019.115146","article-title":"Precise Structures and Anti-Intrinsic Tenase Complex Activity of Three Fucosylated Glycosaminoglycans and Their Fragments","volume":"224","author":"Cai","year":"2019","journal-title":"Carbohydr. Polym."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.carbpol.2015.04.002","article-title":"\u03b2-Eliminative Depolymerization of the Fucosylated Chondroitin Sulfate and Anticoagulant Activities of Resulting Fragments","volume":"127","author":"Gao","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"122000","DOI":"10.1016\/j.carbpol.2024.122000","article-title":"Structural Characterization of a Chlorella Heteropolysaccharide by Analyzing Its Depolymerized Product and Finding an Inducer of Human Dendritic Cell Maturation","volume":"333","author":"Yuan","year":"2024","journal-title":"Carbohydr. Polym."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1055\/s-0039-1681047","article-title":"Nonasaccharide Inhibits Intrinsic Factor Xase Complex by Binding to Factor IXa and Disrupting Factor IXa-Factor VIIIa Interactions","volume":"119","author":"Xiao","year":"2019","journal-title":"Thromb. Haemost."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/23\/2\/64\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:25:35Z","timestamp":1760027135000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/23\/2\/64"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,1]]},"references-count":34,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["md23020064"],"URL":"https:\/\/doi.org\/10.3390\/md23020064","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,1]]}}}