{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:23:55Z","timestamp":1789428235875,"version":"build-2803163510"},"reference-count":53,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Natural Science Foundation of China","award":["82073725"],"award-info":[{"award-number":["82073725"]}]},{"name":"the National Natural Science Foundation of China","award":["32471337"],"award-info":[{"award-number":["32471337"]}]},{"name":"the National Natural Science Foundation of China","award":["CZP22002"],"award-info":[{"award-number":["CZP22002"]}]},{"name":"the National Natural Science Foundation of China","award":["CZQ23025"],"award-info":[{"award-number":["CZQ23025"]}]},{"name":"the Fundamental Research Funds for the Central Universities, South-Central Minzu University","award":["82073725"],"award-info":[{"award-number":["82073725"]}]},{"name":"the Fundamental Research Funds for the Central Universities, South-Central Minzu University","award":["32471337"],"award-info":[{"award-number":["32471337"]}]},{"name":"the Fundamental Research Funds for the Central Universities, South-Central Minzu University","award":["CZP22002"],"award-info":[{"award-number":["CZP22002"]}]},{"name":"the Fundamental Research Funds for the Central Universities, South-Central Minzu University","award":["CZQ23025"],"award-info":[{"award-number":["CZQ23025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>(1) Background: Toll-like receptor 2 (TLR2) on platelets is increasingly recognized as a pivotal mediator in infection-induced platelet activation and aggregation, contributing to both inflammatory and thrombotic diseases. Targeting TLR2 on platelets offers a promising therapeutic strategy for inflammatory and thrombotic-related disorders. However, inhibitors targeting platelet TLR2 have not yet been reported. (2) Methods: Platelet aggregation was assessed using a light transmission aggregometer. Platelet activation was evaluated by measuring the release of P-selectin and von Willebrand factor (vWF) via ELISA. Intracellular Ca2+ mobilization was quantified using Fluo 3-AM fluorescence, recorded by flow cytometry. Static platelet adhesion was visualized under a microscope, and the formation of platelet\u2013granulocyte aggregates in human whole blood was analyzed by flow cytometry. (3) Results: Fucosylated glycosaminoglycan (FG) tetradecasaccharide HS14 inhibited the activation and aggregation of human platelets induced by the synthetic bacterial lipopeptide Pam3CSK4 in a concentration-dependent manner. This inhibitory effect gives rise to significant anti-inflammatory and anti-thrombotic activities, as evidenced by reduced platelet adhesion and decreased platelet\u2013granulocyte aggregates formation in human whole blood. (4) Conclusions: This study is the first to identify FG oligosaccharide HS14 as a promising inhibitor of platelet TLR2\/TLR1, demonstrating significant therapeutic potential for inflammatory and thrombotic-related diseases.<\/jats:p>","DOI":"10.3390\/md23030110","type":"journal-article","created":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T04:58:17Z","timestamp":1741064297000},"page":"110","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Fucosylated Glycosaminoglycan Oligosaccharide HS14, Derived from Sea Cucumbers, Is a Novel Inhibitor of Platelet Toll-like Receptor 2"],"prefix":"10.3390","volume":"23","author":[{"given":"Huifang","family":"Sun","sequence":"first","affiliation":[{"name":"School of Chemistry and Materials Science, South-Central Minzu University, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyu","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, South-Central Minzu University, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujuan","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronghua","family":"Yin","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhua","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-3-642-29423-5_1","article-title":"Platelets: Production, Morphology and Ultrastructure","volume":"210","author":"Thon","year":"2012","journal-title":"Handb. 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