{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T02:59:39Z","timestamp":1791687579407,"version":"4.3.4"},"reference-count":52,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T00:00:00Z","timestamp":1756512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institute of Oceanography, Vietnam Academy of Science and Technology","award":["CSCL18.02\/25\u201326"],"award-info":[{"award-number":["CSCL18.02\/25\u201326"]}]},{"name":"Institute of Oceanography, Vietnam Academy of Science and Technology","award":["25-73-20049"],"award-info":[{"award-number":["25-73-20049"]}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["CSCL18.02\/25\u201326"],"award-info":[{"award-number":["CSCL18.02\/25\u201326"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["25-73-20049"],"award-info":[{"award-number":["25-73-20049"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The aim of this study was to investigate the metabolites in Aspergillus subramanianii 1901NT-1.40.2 extract using UPLC-MS, isolate and elucidate the structure of individual compounds, and study the antimicrobial and cytotoxic activities of the isolated compounds. The structures of two previously unreported ergostane triterpenoid aspersubrin A (1) and pyrazine alkaloid ochramide E (2) were established using NMR and HR ESI-MS. The absolute configuration of 1 was determined using quantum chemical calculations. Moreover, the known polyketides sclerolide (3) and sclerin (4); the indolediterpene alkaloid 10,23-dihydro-24,25-dehydroaflavinine (5); the bis-indolyl benzenoid alkaloids kumbicin D (6), asterriquinol D dimethyl ether (7), petromurin C (8); and the cyclopentenedione asterredione (9) were isolated. The effects of compounds 3-9 on the growth and biofilm formation of the yeast-like fungus Candida albicans and the bacteria Staphylococcus aureus and Escherichia coli were investigated. Compounds 5 and 6 inhibited C. albicans growth and biofilm formation at an IC50 of 7\u201310 \u00b5M. Moreover, the effects of compounds 3-9 on non-cancerous H9c2 cardiomyocytes, HaCaT keratinocytes, MCF-10A breast epithelial cells, and breast cancer MCF-7 and MDA-MB-231 cells were also investigated. Compound 8 (10 \u00b5M) significantly decreased the viability of MCF-7 cells, inhibited colony formation, and arrested cell cycle progression and proliferation in monolayer culture. Moreover, 8 significantly decreased the area of MCF-7 3D spheroids by approximately 30%. A competitive test with 4-hydroxytamoxyfen and molecular docking showed that estrogen receptors (ER\u03b2 more than ER\u03b1) were involved in the anticancer effect of petromurin C (8).<\/jats:p>","DOI":"10.3390\/md23090353","type":"journal-article","created":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T16:39:01Z","timestamp":1756744741000},"page":"353","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Secondary Metabolites of the Marine Sponge-Derived Fungus Aspergillus subramanianii 1901NT-1.40.2 and Their Antimicrobial and Anticancer Activities"],"prefix":"10.3390","volume":"23","author":[{"given":"Olga O.","family":"Khmel","sequence":"first","affiliation":[{"name":"G.B. Elyakov Pacific Institute of Bioorganic Chemistry Far Eastern Branch of Russian Academy of Science, Prospect 100-letiya Vladivostoka, 159, Vladivostok 690022, Russia"},{"name":"Institute of Biotechnology, Bioengineering and Food Systems, Advanced Engineering School, Far Eastern Federal University, 10 Ajax Bay, Russky Island, Vladivostok 690922, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6816-3605","authenticated-orcid":false,"given":"Anton N.","family":"Yurchenko","sequence":"additional","affiliation":[{"name":"G.B. Elyakov Pacific Institute of Bioorganic Chemistry Far Eastern Branch of Russian Academy of Science, Prospect 100-letiya Vladivostoka, 159, Vladivostok 690022, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4582-9360","authenticated-orcid":false,"given":"Phan Thi Hoai","family":"Trinh","sequence":"additional","affiliation":[{"name":"Institute of Oceanography, Vietnam Academy of Science and Technology, Nha Trang 650000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ngo Thi Duy","family":"Ngoc","sequence":"additional","affiliation":[{"name":"Institute of Oceanography, Vietnam Academy of Science and Technology, Nha Trang 650000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4969-0594","authenticated-orcid":false,"given":"Vo Thi Dieu","family":"Trang","sequence":"additional","affiliation":[{"name":"Institute of Oceanography, Vietnam Academy of Science and Technology, Nha Trang 650000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6917-4530","authenticated-orcid":false,"given":"Huynh Hoang Nhu","family":"Khanh","sequence":"additional","affiliation":[{"name":"Institute of Oceanography, Vietnam Academy of Science and Technology, Nha Trang 650000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandr S.","family":"Antonov","sequence":"additional","affiliation":[{"name":"G.B. Elyakov Pacific Institute of Bioorganic Chemistry Far Eastern Branch of Russian Academy of Science, Prospect 100-letiya Vladivostoka, 159, Vladivostok 690022, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin A.","family":"Drozdov","sequence":"additional","affiliation":[{"name":"G.B. Elyakov Pacific Institute of Bioorganic Chemistry Far Eastern Branch of Russian Academy of Science, Prospect 100-letiya Vladivostoka, 159, Vladivostok 690022, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1727-6164","authenticated-orcid":false,"given":"Roman S.","family":"Popov","sequence":"additional","affiliation":[{"name":"G.B. Elyakov Pacific Institute of Bioorganic Chemistry Far Eastern Branch of Russian Academy of Science, Prospect 100-letiya Vladivostoka, 159, Vladivostok 690022, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalya Y.","family":"Kim","sequence":"additional","affiliation":[{"name":"G.B. Elyakov Pacific Institute of Bioorganic Chemistry Far Eastern Branch of Russian Academy of Science, Prospect 100-letiya Vladivostoka, 159, Vladivostok 690022, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitrii V.","family":"Berdyshev","sequence":"additional","affiliation":[{"name":"G.B. 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