{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:46:51Z","timestamp":1790632011116,"version":"4.1.0"},"reference-count":61,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education","award":["RS-2019-NR040078"],"award-info":[{"award-number":["RS-2019-NR040078"]}]},{"name":"Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education","award":["2025-RISE-17-001"],"award-info":[{"award-number":["2025-RISE-17-001"]}]},{"name":"Regional Innovation System and Education (RISE) program through the (Jeju Rise Center), funded by the Ministry of Education (MOE) and the Jeju Special-Governing Province, Republic of Korea","award":["RS-2019-NR040078"],"award-info":[{"award-number":["RS-2019-NR040078"]}]},{"name":"Regional Innovation System and Education (RISE) program through the (Jeju Rise Center), funded by the Ministry of Education (MOE) and the Jeju Special-Governing Province, Republic of Korea","award":["2025-RISE-17-001"],"award-info":[{"award-number":["2025-RISE-17-001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Ulcerative colitis (UC) is a chronic inflammatory bowel disease marked by disruption of the intestinal barrier and gut microbiota imbalance, leading to significant impairment in patient quality of life. This study investigated the therapeutic efficacy of a synbiotic formulation composed of purified fucoidan from bloom-forming Sargassum horneri and the probiotic Lactobacillus plantarum in a dextran sulfate sodium (DSS)-induced zebrafish model of UC. Polysaccharides from S. horneri were extracted using Celluclast-assisted extraction and fractionated via DEAE anion-exchange chromatography, resulting in six fucoidan fractions. The sixth fraction (SH-F), with a molecular weight of 254 kDa, showed the highest fucose, sulfate contents, and demonstrated the highest effect on promoting L. plantarum growth. Structural analysis revealed that SH-F contained \u03b1-L-Fucp-(1\u21923), \u03b1-L-Fucp-(1\u21924), \u03b2-D-Galp-(1\u21922,3,4), \u03b1-L-Fucp-(1\u21923,4), and terminal \u03b1-L-Fucp residues where Fuc1(SO3)1, Gal1Fuc1(SO3)1, and Fuc2(SO3)2 were the most common glycans. Synbiotic administration significantly attenuated DSS-induced colonic shrinkage, inhibited pro-inflammatory cytokines (IL-6, TNF-\u0251, and IL-1\u03b2), restored tight junction proteins (ZO-1, occludin), and downregulated the iNOS, COX2, and NF-\u03baB signaling pathway in adult zebrafish. 16S rRNA gene sequencing revealed restoration of gut microbial diversity and increased abundance of beneficial bacterial taxa to improve DSS-induced UC. These findings highlight the potential synergistic effects of SH-F and L. plantarum as a combinatorial strategy to regulate gut inflammation and enhance epithelial barrier function, potentially offering new insights and therapeutic opportunities for UC intervention.<\/jats:p>","DOI":"10.3390\/md23100372","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T13:48:05Z","timestamp":1758808085000},"page":"372","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Synergistic Role of Sargassum horneri Fucoidan and Lactobacillus plantarum: Microbiome and Gut Barrier Restoration in Zebrafish Colitis"],"prefix":"10.3390","volume":"23","author":[{"given":"N. M.","family":"Liyanage","sequence":"first","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea"},{"name":"Department of Medicine, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. P.","family":"Nagahawatta","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. H. A. C. K.","family":"Jayawardhana","sequence":"additional","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Geon","family":"Je","sequence":"additional","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Yiqiao","sequence":"additional","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengqi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Sang","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung Yuk","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3299-7266","authenticated-orcid":false,"given":"You-Jin","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2769","DOI":"10.1016\/S0140-6736(17)32448-0","article-title":"Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: A systematic review of population-based studies","volume":"390","author":"Ng","year":"2017","journal-title":"Lancet"},{"key":"ref_2","first-page":"174","article-title":"Use of Synbiotics for Ulcerative Colitis Treatment","volume":"15","author":"Roselli","year":"2020","journal-title":"Curr. 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