{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T04:42:28Z","timestamp":1760848948310,"version":"build-2065373602"},"reference-count":46,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:00:00Z","timestamp":1760659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017599","name":"Science and Technology Program of Zhejiang Province","doi-asserted-by":"crossref","award":["LGG22D060001","LTGC24D060001"],"award-info":[{"award-number":["LGG22D060001","LTGC24D060001"]}],"id":[{"id":"10.13039\/501100017599","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Key Program of Science and Technology of Ningbo","award":["2023Z118"],"award-info":[{"award-number":["2023Z118"]}]},{"DOI":"10.13039\/501100004387","name":"Ningbo University Li Da San Ye Yao Zhen and Li Benjun Marine Biomedical New Drug Research and Development Special Fund","doi-asserted-by":"publisher","award":["2025-03"],"award-info":[{"award-number":["2025-03"]}],"id":[{"id":"10.13039\/501100004387","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ningbo Public Welfare Science and Technology Program","award":["2022S161"],"award-info":[{"award-number":["2022S161"]}]},{"name":"One Health Interdisciplinary Research Project, Ningbo University","award":["HY2024010"],"award-info":[{"award-number":["HY2024010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>An integrated microalgae biorefinery producing high-purity xanthophylls using a sustainable and efficient strategy still faces critical challenges. In this study, the microalga Amphidinium carterae can accumulate peridinin and diadinoxanthin cycle carotenoids. Notably, valorization of wet A. carterae using integrated preparation of peridinin and diadinoxanthin cycle carotenoids was developed, containing four main steps including microalgae cultivation, solvent extraction, octadecylsilyl open-column chromatography, and ethanol precipitation for the first time. Under the optimum integrated preparation conditions, the purities of obtained peridinin, diadinoxanthin, and diatoxanthin were all more than 95%, with total recovery rates of approximately 70%, 51%, and 74%, respectively. Based on nuclear magnetic resonance techniques, the purified peridinin, diadinoxanthin, and diatoxanthin were identified as all-trans-peridinin, all-trans-diadinoxanthin, and all-trans-diatoxanthin, respectively. In all, the developed method may hold significant implications for future purification of peridinin and diadinoxanthin cycle carotenoids, as well as for the integrated biorefinery of wet A. carterae.<\/jats:p>","DOI":"10.3390\/md23100405","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T14:29:17Z","timestamp":1760711357000},"page":"405","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Valorization of Amphidinium carterae for Integrated Preparation of Peridinin and Diadinoxanthin Cycle Carotenoids"],"prefix":"10.3390","volume":"23","author":[{"given":"Yi","family":"Li","sequence":"first","affiliation":[{"name":"College of Food Sciences and Engineering, Ningbo University, Ningbo 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gengjie","family":"Zhuang","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Ningbo University, Ningbo 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Sciences and Engineering, Ningbo University, Ningbo 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Cui","sequence":"additional","affiliation":[{"name":"Health Science Center, Ningbo University, Ningbo 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiwei","family":"Hong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China"},{"name":"Department of Applied Biology, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2058-5451","authenticated-orcid":false,"given":"Jianhua","family":"Fan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China"},{"name":"Department of Applied Biology, East China University of Science and Technology, Shanghai 200237, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0774-8479","authenticated-orcid":false,"given":"Jinrong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Sciences and Engineering, Ningbo University, Ningbo 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0257-0157","authenticated-orcid":false,"given":"Xiaojun","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.1080\/10408398.2018.1455030","article-title":"High-value bioproducts from microalgae: Strategies and progress","volume":"59","author":"Liang","year":"2019","journal-title":"Cri. 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