{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T18:18:04Z","timestamp":1774635484020,"version":"3.50.1"},"reference-count":39,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T00:00:00Z","timestamp":1760745600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT","doi-asserted-by":"publisher","award":["RS-2024-00348214"],"award-info":[{"award-number":["RS-2024-00348214"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011705","name":"Korea Institute of Marine Science and Technology Promotion","doi-asserted-by":"publisher","award":["20220488"],"award-info":[{"award-number":["20220488"]}],"id":[{"id":"10.13039\/501100011705","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>An ethyl acetate extract from the deep-sea bacterium Galbibacter orientalis strain ROD011 (GOEE), collected from international waters, was investigated as a potential anti-inflammatory agent. In lipopolysaccharide (LPS)-stimulated murine macrophages, nitric oxide (NO) production fell by 72\u201387% at 5\u201320 \u00b5g\/mL GOEE without detectable cytotoxicity. Cyclooxygenase-2 (COX-2 protein abundance decreased in a dose-dependent manner and was nearly absent at 20 \u00b5g\/mL. In zebrafish embryos, survival was maintained up to 40 \u00b5g\/mL, and LPS-induced signals were attenuated; the cell-death rate declined from 10 \u00b5g\/mL onward, and at 20 \u00b5g\/mL GOEE, reactive oxygen species (ROS) and NO decreased by 85% and 27%, respectively. To explain these effects, untargeted metabolomics with pathway enrichment and network mapping were performed in LPS-driven macrophages. Of the 58 KEGG pathways evaluated, 18 reached significance, notably purine and pyrimidine metabolism, vitamin B6 metabolism, and the one-carbon pool via folate. Coordinated shifts also involved amino-acid\/tricarboxylic acid (TCA)-cycle linkages, glutathione and glyoxylate\/dicarboxylate, and sphingolipid pathways. Network analysis identified hubs that were concomitantly reprogrammed. Collectively, GOEE achieved multi-level suppression of inflammatory outputs while preserving viability, and the metabolomic signature provides a mechanistic scaffold for its action. These findings nominate a deep-sea microbial extract as a promising anti-inflammatory lead and motivate fractionation and targeted validation of the highlighted metabolic nodes.<\/jats:p>","DOI":"10.3390\/md23100409","type":"journal-article","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:44:10Z","timestamp":1760957050000},"page":"409","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["High-Seas Marine Microorganism Delivers an Extract That Dampens LPS-Driven Pro-Inflammatory Signaling: Galbibacter orientalis Strain ROD011"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8926-9449","authenticated-orcid":false,"given":"Minji","family":"Kim","sequence":"first","affiliation":[{"name":"Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3299-7266","authenticated-orcid":false,"given":"You-Jin","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bomi","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2465-8013","authenticated-orcid":false,"given":"Young-Mog","family":"Kim","sequence":"additional","affiliation":[{"name":"Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea"},{"name":"Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6186-5492","authenticated-orcid":false,"given":"Jae-Il","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minkyeong","family":"Choi","sequence":"additional","affiliation":[{"name":"Laboratories of Marine New Drugs, Redone Technologies Co., Ltd., Jangseong-gun 57247, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0451-0959","authenticated-orcid":false,"given":"Sohee","family":"Kim","sequence":"additional","affiliation":[{"name":"Laboratories of Marine New Drugs, Redone Technologies Co., Ltd., Jangseong-gun 57247, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8176-0658","authenticated-orcid":false,"given":"Jihye","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratories of Marine New Drugs, Redone Technologies Co., Ltd., Jangseong-gun 57247, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5680-605X","authenticated-orcid":false,"given":"Jimin","family":"Hyun","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"273","DOI":"10.3233\/EPL-201028","article-title":"A regional review of genetic resource access and benefit sharing\u2013key issues and research gaps","volume":"51","author":"Morrison","year":"2021","journal-title":"Environ. 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