{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T22:52:30Z","timestamp":1780527150103,"version":"3.54.1"},"reference-count":68,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"crossref","award":["23-45-10026"],"award-info":[{"award-number":["23-45-10026"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Belarusian Republican Foundation for Basic Research","award":["B23RNF-093"],"award-info":[{"award-number":["B23RNF-093"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Fucoidan is of considerable interest for the development of drug carriers. The inclusion of fucoidan allows calcium carbonate microparticles in the form of vaterite to acquire new properties, enabling their use in the immobilization of protein preparations. In this work, we investigated the properties of hybrid vaterite microparticles with fucoidan from Fucus vesiculosus obtained by co-precipitation and loaded with recombinant human lactoferrin from goats. The hybrid microparticles had a smaller diameter (3\u20134 \u00b5m), larger surface area (35\u201336 m2g\u22121), smaller pore size (5\u201310 nm average), and more negative \u03b6-potential (\u2212(11\u201313) mV) than the control vaterite microparticles. The incorporation of lactoferrin into the microparticles by co-precipitation in complex with fucoidan was greater than when the protein was adsorbed onto the hybrid microparticles. Microparticles with fucoidan and lactoferrin were stable in acidic environments, released both components over a prolonged period at pH 7.4, and possessed mucoadhesive properties and anticoagulant activity. The antibacterial properties of hybrid microparticles with fucoidan and lactoferrin against Bacillus subtilis were characterized. Microparticles of vaterite with fucoidan can serve as a platform for the microfabrication of effective means of delivering therapeutic proteins.<\/jats:p>","DOI":"10.3390\/md23110428","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T16:09:47Z","timestamp":1762358987000},"page":"428","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Vaterite\/Fucoidan Hybrid Microparticles: Fabrication, Loading of Lactoferrin, Structural Characteristics and Functional Properties"],"prefix":"10.3390","volume":"23","author":[{"given":"Daniil V.","family":"Mosievich","sequence":"first","affiliation":[{"name":"Department of Chemical Enzymology, Faculty of Chemistry, Lomonosov Moscow State University, 1 Leninskiye Gory, 119991 Moscow, Russia"},{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, 1a Malaya Pirogovskaya str., 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8465-0400","authenticated-orcid":false,"given":"Nadezhda G.","family":"Balabushevich","sequence":"additional","affiliation":[{"name":"Department of Chemical Enzymology, Faculty of Chemistry, Lomonosov Moscow State University, 1 Leninskiye Gory, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel I.","family":"Mishin","sequence":"additional","affiliation":[{"name":"Department of Chemical Enzymology, Faculty of Chemistry, Lomonosov Moscow State University, 1 Leninskiye Gory, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3123-1341","authenticated-orcid":false,"given":"Lyubov Y.","family":"Filatova","sequence":"additional","affiliation":[{"name":"Department of Chemical Enzymology, Faculty of Chemistry, Lomonosov Moscow State University, 1 Leninskiye Gory, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina A.","family":"Murina","sequence":"additional","affiliation":[{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, 1a Malaya Pirogovskaya str., 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga V.","family":"Pobeguts","sequence":"additional","affiliation":[{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, 1a Malaya Pirogovskaya str., 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria A.","family":"Galyamina","sequence":"additional","affiliation":[{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, 1a Malaya Pirogovskaya str., 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0086-0970","authenticated-orcid":false,"given":"Ekaterina A.","family":"Obraztsova","sequence":"additional","affiliation":[{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, 1a Malaya Pirogovskaya str., 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0210-5474","authenticated-orcid":false,"given":"Daria V.","family":"Grigorieva","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Belarusian State University, 4 Nezavisimosti Ave., 220030 Minsk, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4737-470X","authenticated-orcid":false,"given":"Irina V.","family":"Gorudko","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Belarusian State University, 4 Nezavisimosti Ave., 220030 Minsk, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9033-0537","authenticated-orcid":false,"given":"Alexey V.","family":"Sokolov","sequence":"additional","affiliation":[{"name":"Smorodintsev Research Institute of Influenza, 15\/17 Prof. Popova Str., 197376 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina V.","family":"Shmeleva","sequence":"additional","affiliation":[{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, 1a Malaya Pirogovskaya str., 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5245-2285","authenticated-orcid":false,"given":"Oleg M.","family":"Panasenko","sequence":"additional","affiliation":[{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, 1a Malaya Pirogovskaya str., 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena V.","family":"Mikhalchik","sequence":"additional","affiliation":[{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, 1a Malaya Pirogovskaya str., 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Haggag, Y.A., Abd Elrahman, A.A., Ulber, R., and Zayed, A. 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