{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:39:24Z","timestamp":1792175964813,"version":"4.4.0"},"reference-count":30,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bio-Based Industries Joint Undertaking under the European Union Horizon 2020 research and innovation program","award":["720755"],"award-info":[{"award-number":["720755"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>While numerous extraction methods have been applied to the brown algae Saccharina latissima, a systematic evaluation of how individual extraction parameters influence the extraction of each target polysaccharide has not previously been reported. Accordingly, this study compared conventional and advanced techniques for extracting fucoidan, laminarin, and alginate from pre-treated biomass. Conventional methods employed diluted acid (0.01 M and 0.1 M HCl), diluted alkali (0.01 M and 0.1 M NaOH), and hot water (121 \u00b0C for 30\/60 min) for extraction. Advanced techniques involved pressurized liquid extraction (PLE) using water and moderate electric field (MEF) extraction with conditions optimized by statistical experimental design. Pre-treatment with aqueous ethanol removed 30% ash and eliminated mannitol, improving extraction selectivity. The results demonstrated fucoidan yields of 31% with 0.01 M HCl and 46% with 0.1 M NaOH, while 0.01 M NaOH facilitated laminarin co-extraction (45%). Alginate, as a mannuronic acid polymer, was obtained at 9% yield with 0.1 M HCl, 42% yield with 0.1 M NaOH, and 27% with pressurized hot water for 30 min. High-temperature, short-duration PLE further improved alginate yield, while MEF showed limited gains due to high ionic content but demonstrated potential under optimized settings. The results support a cascading biorefinery approach in which different polysaccharide fractions can be sequentially obtained, contributing to more sustainable seaweed valorization.<\/jats:p>","DOI":"10.3390\/md23110435","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T09:59:09Z","timestamp":1763027949000},"page":"435","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Impact of Individual Process Parameters on Extraction of Polysaccharides from Saccharina latissima"],"prefix":"10.3390","volume":"23","author":[{"given":"Elmira","family":"Khajavi Ahmadi","sequence":"first","affiliation":[{"name":"Division of Biotechnology and Applied Microbiology, Department of Process and Life Science Engineering, Lund University, Naturvetarv\u00e4gen 22, 223 62 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1877-9943","authenticated-orcid":false,"given":"Said","family":"Al-Hamimi","sequence":"additional","affiliation":[{"name":"Centre for Analysis and Synthesis, Department of Chemistry, Lund University, Naturvetarv\u00e4gen 22, 223 62 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4186-2876","authenticated-orcid":false,"given":"Madeleine","family":"J\u00f6nsson","sequence":"additional","affiliation":[{"name":"Department of Food and Meal Science, Kristianstad University, Elmetorpsv\u00e4gen 15, 291 39 Kristianstad, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roya R. R.","family":"Sardari","sequence":"additional","affiliation":[{"name":"Division of Biotechnology and Applied Microbiology, Department of Process and Life Science Engineering, Lund University, Naturvetarv\u00e4gen 22, 223 62 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Sacramento, M.M.A., Borges, J., Correia, F.J.S., Calado, R., Rodrigues, J.M.M., Patr\u00edcio, S.G., and Mano, J.F. (2022). Green approaches for extraction, chemical modification and processing of marine polysaccharides for biomedical applications. Front. Bioeng. Biotechnol., 10.","DOI":"10.3389\/fbioe.2022.1041102"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Sharma, A., Dubey, S., Singh, K., Mittal, R., Quille, P., and Rajauria, G. (2025). Innovative Processing and Industrial Applications of Seaweed. Phycology, 5.","DOI":"10.3390\/phycology5010010"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1111\/jiec.13539","article-title":"Mapping the global mass flow of seaweed: Cultivation to industry application","volume":"28","author":"Janke","year":"2024","journal-title":"J. Ind. Ecol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Moreira, A.S.P., Gaspar, D., Ferreira, S.S., Correia, A., Vilanova, M., Perrineau, M.-M., Kerrison, P.D., Gachon, C.M.M., Domingues, M.R., and Coimbra, M.A. (2023). Water-Soluble Saccharina latissima Polysaccharides and Relation of Their Structural Characteristics with In Vitro Immunostimulatory and Hypocholesterolemic Activities. Mar. Drugs, 21.","DOI":"10.3390\/md21030183"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1007\/s10811-024-03213-1","article-title":"The sugar kelp Saccharina latissima II: Recent advances in farming and applications","volume":"36","author":"Diehl","year":"2024","journal-title":"J. Appl. Phycol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Kreissig, K.J., Hansen, L.T., Jensen, P.E., Wegeberg, S., Geertz-Hansen, O., and Sloth, J.J. (2021). Characterisation and chemometric evaluation of 17 elements in ten seaweed species from Greenland. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0243672"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"102928","DOI":"10.1016\/j.algal.2022.102928","article-title":"Sequential extraction and fractionation of four polysaccharides from cultivated brown algae Saccharina latissima and Alaria esculenta","volume":"69","author":"Birgersson","year":"2023","journal-title":"Algal Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1016\/j.procbio.2012.06.016","article-title":"Novel procedures for the extraction of fucoidan from brown algae","volume":"47","author":"Hahn","year":"2012","journal-title":"Process Biochem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1002\/jsfa.1754","article-title":"Laminarin in the dietary fibre concept","volume":"84","author":"Damas","year":"2004","journal-title":"J. Sci. Food Agric."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"104142","DOI":"10.1016\/j.tifs.2023.104142","article-title":"Overview of alginate extraction processes: Impact on alginate molecular structure and techno-functional properties","volume":"140","author":"Bojorges","year":"2023","journal-title":"Trends Food Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"da Silva, J., dos Santos, L.C., Iba\u00f1ez, E., and Ferreira, S.R.S. (2025). Green Extraction Methods Applied to the Brown Macroalga Saccharina latissima: Assessing Yield, Total Phenolics, Phlorotannins and Antioxidant Capacity. Foods, 14.","DOI":"10.3390\/foods14061017"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Sund, R., Holdt, S.L., Bang-Berthelsen, C.H., St\u00e9vant, P., Sloth, J.J., and Skipnes, D. (2025). Pulsed electric field processing as an alternative to warm water treatment for the reduction of potentially toxic elements in Saccharina latissima. J. Appl. Phycol., 1\u201313.","DOI":"10.1007\/s10811-025-03596-9"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"114033","DOI":"10.1016\/j.lwt.2022.114033","article-title":"On the use of pulsed electric field technology as a pretreatment to reduce the content of potentially toxic elements in dried Saccharina latissima","volume":"169","author":"Blikra","year":"2022","journal-title":"LWT"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Sardari, R.R.R., Prothmann, J., Gregersen, O., Turner, C., and Nordberg Karlsson, E. (2020). Identification of Phlorotannins in the Brown Algae, Saccharina latissima and Ascophyllum nodosum by Ultra-High-Performance Liquid Chromatography Coupled to High-Resolution Tandem Mass Spectrometry. Molecules, 26.","DOI":"10.3390\/molecules26010043"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2106","DOI":"10.3390\/md9102106","article-title":"Important Determinants for Fucoidan Bioactivity: A Critical Review of Structure-Function Relations and Extraction Methods for Fucose-Containing Sulfated Polysaccharides from Brown Seaweeds","volume":"9","author":"Ale","year":"2011","journal-title":"Mar. Drugs"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1002\/jsfa.2740030305","article-title":"Manufacture of algal chemicals. IV\u2014Laboratory-scale isolation of fucoidin from brown marine algae","volume":"3","author":"Black","year":"1952","journal-title":"J. Sci. Food Agric."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.1007\/s10811-021-02398-z","article-title":"Extraction of laminarin from Saccharina latissima seaweed using cross-flow filtration","volume":"33","author":"Sterner","year":"2021","journal-title":"J. Appl. Phycol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.1007\/s10811-021-02453-9","article-title":"Optimization of citric acid treatment for the sequential extraction of fucoidan and alginate from Sargassum latifolium and their potential antioxidant and Fe(III) chelation properties","volume":"33","author":"Fawzy","year":"2021","journal-title":"J. Appl. Phycol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Gunarathne, R., Zhang, C., Zhou, J., Xin, X., and Lu, J. (2025). Low molecular weight fucoidan: Production methods and their therapeutic applications. Int. J. Biol. Macromol., 318.","DOI":"10.1016\/j.ijbiomac.2025.145309"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Venkatesan, J., Anil, S., and Kim, S.-K. (2017). Chapter 3\u2014Extraction of Sulfated Polysaccharides (Fucoidan) From Brown Seaweed. Seaweed Polysaccharides, Elsevier.","DOI":"10.1016\/B978-0-12-809816-5.00001-3"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2682","DOI":"10.1080\/87559129.2023.2279583","article-title":"Alginates from Brown Seaweeds as a Promising Natural Source: A Review of Its Properties and Health Benefits","volume":"40","author":"Ye","year":"2024","journal-title":"Food Rev. Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1021\/bm010008g","article-title":"Molecular Basis of Ca2+-Induced Gelation in Alginates and Pectins:\u2009 The Egg-Box Model Revisited","volume":"2","author":"Braccini","year":"2001","journal-title":"Biomacromolecules"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Martins, R., Barbosa, A., Advinha, B., Sales, H., Pontes, R., and Nunes, J. (2023). Green Extraction Techniques of Bioactive Compounds: A State-of-the-Art Review. Processes, 11.","DOI":"10.3390\/pr11082255"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Perez-Vazquez, A., Carpena, M., Barciela, P., Cassani, L., Simal-Gandara, J., and Prieto, M.A. (2023). Pressurized Liquid Extraction for the Recovery of Bioactive Compounds from Seaweeds for Food Industry Application: A Review. Antioxidants, 12.","DOI":"10.3390\/antiox12030612"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"8816","DOI":"10.1021\/acssuschemeng.9b00780","article-title":"Moderate Electric Fields as a Potential Tool for Sustainable Recovery of Phenolic Compounds from Pinus pinaster Bark","volume":"7","author":"Genisheva","year":"2019","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Pereira, S.G., Pereira, R.N., Rocha, C.M.R., and Teixeira, J.A. (2023). Electric fields as a promising technology for the recovery of valuable bio compounds from algae: Novel and sustainable approaches. Bioresour. Technol. Rep., 22.","DOI":"10.1016\/j.biteb.2023.101420"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1007\/s10811-021-02624-8","article-title":"Extraction of bioactive compounds from Alaria esculenta with pulsed electric field","volume":"34","author":"Kristbergsson","year":"2022","journal-title":"J. Appl. Phycol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Van Wychen, S., and Laurens, L.M. (2013). Determination of Total Solids and Ash in Algal Biomass, National Renewable Energy Laboratory (NREL). Technical Report NREL\/TP-5100-60956.","DOI":"10.2172\/2205665"},{"key":"ref_29","unstructured":"Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D., and Crocker, D. (2012). Determination of Structural Carbohydrates and Lignin in Biomass: Laboratory Analytical Procedure (LAP), National Renewable Energy Laboratory. Technical Report No. NREL\/TP-510-42618."},{"key":"ref_30","first-page":"826","article-title":"A Simple and High Resolution Ion-Pair HPLC Method for Separation and Simultaneous Determination of Nitrate and Thiocyanate in Different Water Samples","volume":"50","author":"Soleimani","year":"2012","journal-title":"J. Chromatogr. Sci."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/23\/11\/435\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T10:02:37Z","timestamp":1763028157000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/23\/11\/435"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,13]]},"references-count":30,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["md23110435"],"URL":"https:\/\/doi.org\/10.3390\/md23110435","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,13]]}}}