{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T11:03:27Z","timestamp":1765969407852,"version":"3.48.0"},"reference-count":44,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009068","name":"Centre for Biotechnology and Bioengineering","doi-asserted-by":"publisher","award":["AFB240001"],"award-info":[{"award-number":["AFB240001"]}],"id":[{"id":"10.13039\/501100009068","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009068","name":"Fondecyt project","doi-asserted-by":"publisher","award":["1230547"],"award-info":[{"award-number":["1230547"]}],"id":[{"id":"10.13039\/501100009068","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The biomass and lipid production responses of the psychrophilic marine thraustochytrid Thraustochytrium sp. RT2316-16 were assessed in chemically defined media comprising glucose, up to 17 amino acids and up to 9 B-vitamins and mineral salts. Compared to the control medium with all amino acids and B-vitamins (biomass concentration: 7.1 \u00b1 0.1 g L\u22121; total lipid content: 30.4 \u00b1 0.5% of the DW), the growth of RT2316-16 was reduced by more than 50% in the medium that lacked cyanocobalamin or pyridoxamine. The total lipid content of the biomass grown in the absence of vitamins was 63% lower than in the biomass produced in the control medium. The composition of the B-vitamin mixture modulated the fatty acid composition, an effect that may have been related to the availability of dissolved oxygen. In bioreactor culture with the dissolved oxygen level controlled to \u226510% of air saturation, the microorganism consumed all 17 amino acids; 8 of the amino acids were fully consumed within a 0\u201333 h period, in which the specific growth rate was 0.065 h\u22121. Under these culture conditions, the sum of eicosapentaenoic acid and docosahexaenoic acid in the total fatty acid content rose from 15% (at time 0) to 54% (after 95 h). A medium that contained the 9 amino acids that were not preferentially consumed favored the accumulation of total lipids, but reduced biomass growth.<\/jats:p>","DOI":"10.3390\/md23120482","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T10:41:23Z","timestamp":1765968083000},"page":"482","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biomass Growth and Fatty Acid Production by the Marine Thraustochytrium sp. RT2316-16 in Chemically Defined Media"],"prefix":"10.3390","volume":"23","author":[{"given":"Liset","family":"Flores","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, Center of Food Biotechnology and Bioseparations, BIOREN, and Centre of Biotechnology and Bioengineering (CeBiB), Universidad de La Frontera, Temuco 4780000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Paz","family":"Lefiguala","sequence":"additional","affiliation":[{"name":"Doctorate in Engineering Sciences with Specialization in Bioprocess, Universidad de La Frontera, Temuco 4780000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4646-038X","authenticated-orcid":false,"given":"Carolina","family":"Shene","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Center of Food Biotechnology and Bioseparations, BIOREN, and Centre of Biotechnology and Bioengineering (CeBiB), Universidad de La Frontera, Temuco 4780000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Valdebenito, D., Urrutia, S., Leyton, A., Chisti, Y., Asenjo, J.A., and Shene, C. (2023). Nitrogen sources affect the long-chain polyunsaturated fatty acids content in Thraustochytrium sp. RT2316-16. Mar. Drugs, 21.","DOI":"10.3390\/md21010015"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Leyton, A., Flores, L., Shene, C., Chisti, Y., Larama, G., Asenjo, J.A., and Armenta, R.E. (2021). Antarctic thraustochytrids as sources of carotenoids and high-value fatty acids. Mar. Drugs, 19.","DOI":"10.3390\/md19070386"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Leyton, A., Shene, C., Chisti, Y., and Asenjo, J.A. (2022). Production of carotenoids and phospholipids by Thraustochytrium sp. in batch and repeated-batch culture. Mar. Drugs, 20.","DOI":"10.3390\/md20070416"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Flores, L., Shene, C., Asenjo, J.A., and Chisti, Y. (2023). Coenzyme Q in Thraustochytrium sp. RT2316-16: Effect of the medium composition. Mar. Drugs, 21.","DOI":"10.3390\/md21110586"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Flores, L., and Shene, C. (2024). Single amino acids as sole nitrogen source for the production of lipids and coenzyme Q by Thraustochytrium sp. RT2316-16. Microorganisms, 12.","DOI":"10.3390\/microorganisms12071428"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/s40643-025-00937-x","article-title":"Production of squalene and fatty acids by Thraustochytrium sp. RT2316-16: Effects of dissolved oxygen and the medium composition","volume":"12","author":"Paredes","year":"2025","journal-title":"Bioresour. Bioproc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1007\/s00253-006-0419-1","article-title":"Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species: Screening of strains and optimization of omega-3 production","volume":"72","author":"Burja","year":"2006","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"151","DOI":"10.3390\/md18030151","article-title":"The nutritional and pharmacological potential of new Australian thraustochytrids isolated from mangrove sediments","volume":"18","author":"Miranda","year":"2020","journal-title":"Mar. Drugs"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1007\/s11814-011-0287-y","article-title":"The effects of culture condition on the growth property and docosahexaenoic acid production from Thraustochytrium aureum ATCC 34304","volume":"29","author":"Min","year":"2012","journal-title":"Korean J. Chem. Eng."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1007\/s10811-017-1274-4","article-title":"Improvement of culture conditions for cell biomass and fatty acid production by marine thraustochytrid F24-2","volume":"30","author":"Ugalde","year":"2018","journal-title":"J. Appl. Phycol."},{"key":"ref_11","unstructured":"Spickermann, D., van der Werf, M., Reimers, C., and Kleist, C. (2020). Trehalose-Rich Yeast Hydrolysate. (EP 3 744 853 A1), European Patent Application, Available online: https:\/\/patents.google.com\/patent\/EP3744853A1\/en."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"151","DOI":"10.4014\/jmb.2207.07057","article-title":"Yeast extract: Characteristics, production, applications and future perspectives","volume":"33","author":"Tao","year":"2023","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1042\/BST0370242","article-title":"Amino-acid-induced signalling via the SPS-sensing pathway in yeast","volume":"37","author":"Ljungdahl","year":"2009","journal-title":"Biochem. Soc. Trans."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1111\/1462-2920.14978","article-title":"The zoospores of the thraustochytrid Aurantiochytrium limacinum: Transcriptional reprogramming and lipid metabolism associated to their specific functions","volume":"22","author":"Dellero","year":"2020","journal-title":"Environ. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1007\/s10811-022-02726-x","article-title":"Development of a fed-batch fermentation with stepwise aeration to enhance docosahexaenoic acid and carotenoid content in Aurantiochytrium sp. FIKU018","volume":"34","author":"Khumrangsee","year":"2022","journal-title":"J. Appl. Phycol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.algal.2018.10.012","article-title":"The effect of thiamine on the growth and fatty acid content of Aurantiochytrium sp.","volume":"36","author":"Tani","year":"2018","journal-title":"Algal Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/BF00422521","article-title":"B12 and B1 auxotrophy of lower marine phycomycetes","volume":"47","author":"Goldstein","year":"1963","journal-title":"Arch. Mikrobiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1271\/bbb.60308","article-title":"Effect of vitamin B12-enriched thraustochytrids on the population growth of rotifers","volume":"71","author":"Hayashi","year":"2007","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Sen, B., Li, J., Lu, L., Bai, M., He, Y., and Wang, G. (2021). Elemental composition and cell mass quantification of cultured thraustochytrids unveil their large contribution to marine carbon pool. Mar. Drugs, 19.","DOI":"10.3390\/md19090493"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0083-6729(08)61024-9","article-title":"Role of vitamin B12 in the metabolism of microorganisms","volume":"13","author":"Ford","year":"1955","journal-title":"Vitam. Horm."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e1205","DOI":"10.1073\/pnas.1612360114","article-title":"Elucidation of roles for vitamin B12 in regulation of folate, ubiquinone, and methionine metabolism","volume":"114","author":"Romine","year":"2017","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1146\/annurev-marine-120710-100912","article-title":"The role of B vitamins in marine biogeochemistry","volume":"6","author":"Suffridge","year":"2014","journal-title":"Ann. Rev. Mar. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.synbio.2016.01.005","article-title":"Mechanisms of biotin-regulated gene expression in microbes","volume":"1","author":"Satiaputra","year":"2016","journal-title":"Synth. Syst. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s00253-002-1186-2","article-title":"Biotin in microbes, the genes involved in its biosynthesis, its biochemical role and perspectives for biotechnological production","volume":"61","author":"Streit","year":"2003","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Ali, M.K., Sen, B., He, Y., Bai, M., and Wang, G. (2022). Media supplementation with mannitol and biotin enhances squalene production of Thraustochytrium ATCC 26185 through increased glucose uptake and antioxidative mechanisms. Molecules, 27.","DOI":"10.3390\/molecules27082449"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1016\/j.bbapap.2010.12.006","article-title":"Vitamin B6 salvage enzymes: Mechanism, structure and regulation","volume":"1814","author":"Contestabile","year":"2011","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Parra, M., Stahl, S., and Hellmann, H. (2018). Vitamin B6 and Its Role in Cell Metabolism and Physiology. Cells, 22.","DOI":"10.3390\/cells7070084"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4362","DOI":"10.1128\/JB.187.13.4362-4371.2005","article-title":"Homocysteine toxicity in Escherichia coli is caused by a perturbation of branched-chain amino acid biosynthesis","volume":"187","author":"Tuite","year":"2005","journal-title":"J. Bacteriol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1002\/j.1460-2075.1991.tb07986.x","article-title":"Proofreading in vivo: Editing of homocysteine by methionyl-tRNA synthetase in the yeast Saccharomyces cerevisiae","volume":"10","author":"Jakubowski","year":"1991","journal-title":"EMBO J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1858","DOI":"10.1099\/mic.0.026609-0","article-title":"Homocysteine editing and growth inhibition in Escherichia coli","volume":"155","author":"Sikora","year":"2009","journal-title":"Microbiology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/BF01800662","article-title":"Riboflavin-responsive defects of beta-oxidation","volume":"8","author":"Gregersen","year":"1985","journal-title":"J. Inherit. Metab. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/S1097-2765(04)00148-0","article-title":"Yeast adapt to near-freezing temperatures by STRE\/Msn2,4-dependent induction of trehalose synthesis and certain molecular chaperones","volume":"13","author":"Kandror","year":"2004","journal-title":"Mol. Cell"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/S1097-2765(00)80064-7","article-title":"Multiple effects of trehalose on protein folding in vitro and in vivo","volume":"1","author":"Singer","year":"1998","journal-title":"Mol. Cell"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"24261","DOI":"10.1074\/jbc.M101487200","article-title":"Trehalose accumulation during cellular stress protects cells and cellular proteins from damage by oxygen radicals","volume":"276","author":"Banaroudj","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"107897","DOI":"10.1016\/j.biotechadv.2021.107897","article-title":"Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.","volume":"55","author":"Chi","year":"2022","journal-title":"Biotechnol. Adv."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Patel, A., Rova, U., Christakopoulos, P., and Matsakas, L. (2020). Assessment of Fatty Acids Profile and Omega-3 Polyunsaturated Fatty Acid Production by the Oleaginous Marine Thraustochytrid Aurantiochytrium sp. T66 Cultivated on Volatile Fatty Acids. Biomolecules, 10.","DOI":"10.3390\/biom10050694"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"8102","DOI":"10.1128\/AEM.02294-12","article-title":"Sequential use of nitrogen compounds by Saccharomyces cerevisiae during wine fermentation: A model based on kinetic and regulation characteristics of nitrogen permeases","volume":"78","author":"Nidelet","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1104\/pp.81.3.774","article-title":"Increased Arginine Biosynthesis during Phosphorus Deficiency: A Response to the Increased Ammonia Content of Leaves","volume":"81","author":"Rabe","year":"1986","journal-title":"Plant Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1128\/jb.115.1.284-290.1973","article-title":"Use of external, biosynthetic, and organellar arginine by Neurospora","volume":"115","author":"Subramanian","year":"1973","journal-title":"J. Bacteriol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Koopmann, I.K., M\u00fcller, B.A., and Labes, A. (2023). Screening of a thraustochytrid strain collection for carotenoid and squalene production characterized by cluster analysis, comparison of 18S rRNA gene sequences, growth behavior, and morphology. Mar. Drugs, 21.","DOI":"10.3390\/md21040204"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3695","DOI":"10.1073\/pnas.062526999","article-title":"Metabolic efficiency and amino acid composition in the proteomes of Escherichia coli and Bacillus subtilis","volume":"99","author":"Akashi","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1002\/ejlt.201200417","article-title":"Production of lipids and docosahexaenoic acid (DHA) by a native Thraustochytrium strain","volume":"115","author":"Shene","year":"2013","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1021\/ac60147a030","article-title":"Use of dinitrosalicylic acid reagent for determination of reducing sugar","volume":"31","author":"Miller","year":"1959","journal-title":"J. Anal. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method for total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/23\/12\/482\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T10:46:23Z","timestamp":1765968383000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/23\/12\/482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,17]]},"references-count":44,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["md23120482"],"URL":"https:\/\/doi.org\/10.3390\/md23120482","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,17]]}}}