{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T18:06:29Z","timestamp":1793383589142,"version":"4.5.0"},"reference-count":25,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T00:00:00Z","timestamp":1768003200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","award":["88887.675680\/2022-00"],"award-info":[{"award-number":["88887.675680\/2022-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2021\/11936-3"],"award-info":[{"award-number":["2021\/11936-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["401 2023\/01554-1"],"award-info":[{"award-number":["401 2023\/01554-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The National Council for Scientific and Technological Development","award":["301608\/2022-403 9"],"award-info":[{"award-number":["301608\/2022-403 9"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Fisheries bycatch, while representing a major ecological concern due to the incidental capture of non-target species, also constitutes an underexplored source of marine biomass with biotechnological potential. This study aimed to generate and characterize bioactive peptides from the muscle tissue of three common bycatch species from the Brazilian coast: Paralonchurus brasiliensis, Micropogonias furnieri, and Hepatus pudibundus. Muscle homogenates were hydrolyzed using either Alcalase or Protamex to produce peptide-rich hydrolysates, which were analyzed through SDS-PAGE, HPLC-UV, MALDI-TOF, and LC-MS\/MS. De novo sequencing and bioinformatic analyses predicted bioactivities that were subsequently validated by in vitro assays. The results demonstrated that enzyme selection strongly influenced both peptide profiles and bioactivity. The Protamex hydrolysate of P. brasiliensis (PBP) exhibited potent antifungal activity, inhibiting Candida albicans growth by 81%, whereas the Alcalase hydrolysate (PBA) showed moderate inhibition of Staphylococcus aureus (29%). No significant effect was observed against Escherichia coli. Overall, this study highlights a sustainable strategy for the valorization of fisheries bycatch through the production of bioactive marine peptides and identifies P. brasiliensis hydrolyzed with Protamex as a promising source of anti-Candida peptides for pharmaceutical and nutraceutical applications.<\/jats:p>","DOI":"10.3390\/md24010036","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T08:20:37Z","timestamp":1768206037000},"page":"36","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Proteomic and Functional Characterization of Antimicrobial Peptides Derived from Fisheries Bycatch via Enzymatic Hydrolysis"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8887-4607","authenticated-orcid":false,"given":"Vicky Balesteros S. Blumen","family":"Galendi","sequence":"first","affiliation":[{"name":"Botucatu Medical School (FMB), S\u00e3o Paulo State University (UNESP), Botucatu 18618-687, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9633-7219","authenticated-orcid":false,"given":"Guilherme Rabelo","family":"Coelho","sequence":"additional","affiliation":[{"name":"Laboratory of Toxinology, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of S\u00e3o Paulo (USP), S\u00e3o Paulo 05508-220, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4280-896X","authenticated-orcid":false,"given":"Let\u00edcia","family":"Murback","sequence":"additional","affiliation":[{"name":"Botucatu Medical School (FMB), S\u00e3o Paulo State University (UNESP), Botucatu 18618-687, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8526-1052","authenticated-orcid":false,"given":"Wagner C.","family":"Valenti","sequence":"additional","affiliation":[{"name":"Aquaculture Center (CAUNESP), Jaboticabal Campus, S\u00e3o Paulo State University (UNESP), Jaboticabal 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7885-8849","authenticated-orcid":false,"given":"Tavani Rocha","family":"Camargo","sequence":"additional","affiliation":[{"name":"SAMPLE\u2014Innovation and Biotechnology in Animal Production, Registro 11900-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0654-639X","authenticated-orcid":false,"given":"Marcia Regina","family":"Franzolin","sequence":"additional","affiliation":[{"name":"Butantan Institute, S\u00e3o Paulo 05503-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2406-0860","authenticated-orcid":false,"given":"Daniel Carvalho","family":"Pimenta","sequence":"additional","affiliation":[{"name":"Butantan Institute, S\u00e3o Paulo 05503-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6952-0512","authenticated-orcid":false,"suffix":"Jr.","given":"Rui Seabra","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Botucatu Medical School (FMB), S\u00e3o Paulo State University (UNESP), Botucatu 18618-687, SP, Brazil"},{"name":"Center for the Study of Venoms and Venomous Animals (CEVAP), Botucatu Campus, S\u00e3o Paulo State University (UNESP), Botucatu 18618-687, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1111\/lam.12347","article-title":"Investigation of Biotechnological Potential of Sponge-Associated Bacteria Collected in Brazilian Coast","volume":"60","author":"Santos","year":"2015","journal-title":"Lett. 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