{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:26:07Z","timestamp":1787919967236,"version":"build-2784847793"},"reference-count":40,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"crossref","award":["CIAPOS\/2022\/70"],"award-info":[{"award-number":["CIAPOS\/2022\/70"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004837","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["PID2022-139110OA-I00"],"award-info":[{"award-number":["PID2022-139110OA-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014180","name":"Regional Government of Castilla y Le\u00f3n","doi-asserted-by":"crossref","award":["UIC393"],"award-info":[{"award-number":["UIC393"]}],"id":[{"id":"10.13039\/501100014180","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100014180","name":"Regional Government of Castilla y Le\u00f3n","doi-asserted-by":"crossref","award":["UIC 379"],"award-info":[{"award-number":["UIC 379"]}],"id":[{"id":"10.13039\/501100014180","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>The conversion of biogas into high-value chemicals for pharmaceutical, cosmetic, and nutraceutical markets offers an attractive alternative to conventional fossil-based production routes, enabling circular value chains with significant socio-economic impact. This study evaluated the valorization of biogas into osmolyte and carotenoid compounds with market prices ranging from 1000 to 7000 $\u00b7kg\u22121. Specifically, an algal\u2013methanotrophic co-culture operated under saline conditions, preventing external microbial contamination and stimulating osmolytes and carotenoids, was assessed for its capacity to simultaneously remove methane (CH4) and carbon dioxide (CO2), with efficiencies of 92 and 89%, respectively. while producing ectoine, hydroxyectoine, lutein, \u03b2-carotene, and astaxanthin. Shotgun metagenomic analyses identified the key microorganisms driving the process, predominantly alkaliphilic and halophilic green algae (Chlorella, Dunaliella) and cyanobacteria (Leptolyngbya), and halotolerant methanotrophs (Methylotuvimicrobium) and methylotrophs (Methylophaga). Metagenomics further revealed the presence of key metabolisms related to C1 utilization and biosynthetic genes associated with carotenoid and osmolyte production, confirming the metabolic potential of the consortium to convert biogas-derived carbon directly into high-value compounds. Overall, these results demonstrate the feasibility of an efficient, biologically driven bio-platform capable of transforming greenhouse gas-rich waste streams into economically relevant bioactive molecules, contributing to global priorities in sustainable biomass-to-biochemical innovation.<\/jats:p>","DOI":"10.3390\/md24020081","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T09:22:46Z","timestamp":1771320166000},"page":"81","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Harnessing Biogas into High-Value Chemicals: The Role of Algal\u2013Methanotrophic Co-Cultures"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7494-8265","authenticated-orcid":false,"given":"Rebecca","family":"Serna-Garc\u00eda","sequence":"first","affiliation":[{"name":"Institute of Sustainable Processes, University of Valladolid, 47011 Valladolid, Spain"},{"name":"Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s\/n, 47011 Valladolid, Spain"},{"name":"CALAGUA\u2013Unidad Mixta UV-UPV, Departament Enginyeria Qu\u00edmica, Universitat de Val\u00e8ncia, Avinguda de la Universitat s\/n, 46100 Burjassot, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5359-1754","authenticated-orcid":false,"given":"Ysis","family":"Lanzoni","sequence":"additional","affiliation":[{"name":"Institute of Sustainable Processes, University of Valladolid, 47011 Valladolid, Spain"},{"name":"Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s\/n, 47011 Valladolid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7881-1143","authenticated-orcid":false,"given":"Octavio","family":"Garc\u00eda-Depraect","sequence":"additional","affiliation":[{"name":"Institute of Sustainable Processes, University of Valladolid, 47011 Valladolid, Spain"},{"name":"Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s\/n, 47011 Valladolid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1207-6275","authenticated-orcid":false,"given":"Raul","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Institute of Sustainable Processes, University of Valladolid, 47011 Valladolid, Spain"},{"name":"Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s\/n, 47011 Valladolid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7116-9055","authenticated-orcid":false,"given":"Sara","family":"Cantera","sequence":"additional","affiliation":[{"name":"Institute of Sustainable Processes, University of Valladolid, 47011 Valladolid, Spain"},{"name":"Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s\/n, 47011 Valladolid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Neilan, B., Passarini, M.R.Z., Singh, P.K., and Kumar, A. (2023). Microalgal Bio-pigments: Production and Enhancement Strategies to Enrich Microalgae-Derived Pigments. Cyanobacterial Biotechnology in the 21st Century, Springer.","DOI":"10.1007\/978-981-99-0181-4"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.algal.2017.09.016","article-title":"Rapid assessment of algal biomass and pigment contents using diffuse reflectance spectroscopy and chemometrics","volume":"27","author":"Duppeti","year":"2017","journal-title":"Algal. Res."},{"key":"ref_3","unstructured":"BCC Research LLC (2025, December 12). Global Carotenoids Market Size, Share & Growth Analysis Report. Available online: https:\/\/www.bccresearch.com\/market-research\/food-and-beverage\/the-global-market-for-carotenoids.html."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Sun, H., Wang, Y., He, Y., Liu, B., Mou, H., Chen, F., and Yang, S. (2023). Microalgae-Derived Pigments for the Food Industry. Mar. Drugs, 21.","DOI":"10.3390\/md21020082"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"113841","DOI":"10.1016\/j.foodres.2023.113841","article-title":"Astaxanthin from microalgae: A review on structure, biosynthesis, production strategies and application","volume":"176","author":"Debnath","year":"2024","journal-title":"Food. Res. Int."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Silva, S.C., Ferreira, I.C.F.R., Dias, M.M., and Barreiro, M.F. (2020). Microalgae-Derived Pigments: A 10-Year Bibliometric Review and Industry and Market Trend Analysis. Molecules, 25.","DOI":"10.3390\/molecules25153406"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"128875","DOI":"10.1016\/j.biortech.2023.128875","article-title":"Luiten production from microalgae: A review","volume":"376","author":"Fu","year":"2023","journal-title":"Bioresour. Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/s12934-021-01567-6","article-title":"Microbial production of ectoine and hydroxyectoine as high-value chemicals","volume":"20","author":"Liu","year":"2021","journal-title":"Microb. Cell Fact."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1556\/066.2025.00134","article-title":"A review of the research progress on co-cultivation of symbiotic methanotroph bacteria with algae","volume":"54","author":"Zhang","year":"2025","journal-title":"Acta Aliment."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"127418","DOI":"10.1016\/j.chemosphere.2020.127418","article-title":"Methanotroph-microalgae co-culture for greenhouse gas mitigation: Effect of initial biomass ratio and methane concentration","volume":"259","author":"Revah","year":"2020","journal-title":"Chemosphere"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Roberts, N., Hilliard, M., He, Q.P., and Wang, J. (2020). A Microalgae-Methanotroph Coculture is a Promising Platform for Fuels and Chemical Production from Wastewater. Front. Energy Res., 8.","DOI":"10.3389\/fenrg.2020.563352"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3669","DOI":"10.1021\/acs.est.1c06831","article-title":"Mixotrophic cultivation of microalgae using biogas as the substrate","volume":"56","author":"Li","year":"2022","journal-title":"Environ. Sci. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Sang, Y., Xie, Z., Li, L., Wang, O., Zheng, S., and Liu, F. (2024). Biohydrogen Production from Methane-Derived Biomass of Methanotroph and Microalgae by Clostridium. Fermentation, 10.","DOI":"10.3390\/fermentation10080383"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"124223","DOI":"10.1016\/j.jenvman.2025.124223","article-title":"Ectoine production through a marine methanotroph-microalgae culture allows complete biogas valorization","volume":"375","year":"2025","journal-title":"J. Environ. Manag."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Souabi, S., Anouzla, A., Yadav, S., Singh, V.P., and Yadava, R.N. (2025). Photosynthetic Biogas Upgrading with Microalgae Coupled with Nutrient Recovery from Digestate. Wastewater Treatment Plants. Water Science and Technology Library, Springer.","DOI":"10.1007\/978-3-031-87461-1"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"103835","DOI":"10.1016\/j.algal.2024.103835","article-title":"From biogas to biomethane: Evaluating the role of microalgae in sustainable energy production\u2014A review","volume":"85","author":"Wojaczek","year":"2025","journal-title":"Algal Res."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Oshkin, I.Y., Tikhonova, E.N., Suleimanov, R.Z., Ashikhmin, A.A., Ivanova, A.A., Pimenov, N.V., and Dedysh, S.N. (2023). All Kinds of Sunny Colors Synthesized from Methane: Genome-Encoded Carotenoid Production by Methylomonas Species. Microorganisms, 11.","DOI":"10.3390\/microorganisms11122865"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"126398","DOI":"10.1016\/j.syapm.2023.126398","article-title":"Methylomonas rapida sp. nov., a novel species of fast-growing, carotenoid-producing obligate methanotrophs with high biotechnological potential","volume":"46","author":"Tikhonova","year":"2023","journal-title":"Syst. Appl. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.greenca.2024.10.001","article-title":"Recent advances in microalgae-driven carbon capture, utilization, and storage: Strain engineering through adaptative laboratory evolution and microbiome optimization","volume":"3","author":"He","year":"2025","journal-title":"Green Carbon"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"102119","DOI":"10.1016\/j.algal.2020.102119","article-title":"Two-stage bioprocess for hyper-production of lutein from microalga Chlorella sorokiniana FZU60: Effects of temperature, light intensity, and operation strategies","volume":"52","author":"Ma","year":"2020","journal-title":"Algal Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"128583","DOI":"10.1016\/j.biortech.2023.128583","article-title":"Bioprocess development to enhance biomass and lutein production from Chlorella sorokiniana Kh12","volume":"370","author":"Vadrale","year":"2023","journal-title":"Bioresour. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"126816","DOI":"10.1016\/j.biortech.2022.126816","article-title":"Exploring engineering reduced graphene oxide-titanium dioxide (RGO-TiO2) nanoparticles treatment to effectively enhance lutein biosynthesis with Chlorella sorokiniana F31 under different light intensity","volume":"348","author":"Zhao","year":"2022","journal-title":"Bioresour. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Mauffrey, F., Martineau, C., and Villemur, R. (2015). Importance of the Two Dissimilatory (Nar) Nitrate Reductases in the Growth and Nitrate Reduction of the Methylotrophic Marine Bacterium Methylophaga nitratireducenticrescens JAM1. Front. Microb., 6.","DOI":"10.3389\/fmicb.2015.01475"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1093\/ismeco\/ycaf183","article-title":"Targeted metagenomics using probe capture detect a larger diversity of nitrogen and methane cycling genes in complex microbial communities than traditional metagenomics","volume":"5","author":"Siljanen","year":"2022","journal-title":"ISME Commun."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.1038\/ncomms3785","article-title":"Highly efficient methane biocatalysis revealed in a methanotrophic bacterium","volume":"4","author":"Kalyuzhnaya","year":"2013","journal-title":"Nature Commun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2762","DOI":"10.1099\/ijs.0.64955-0","article-title":"Methylohalomonas lacus gen. nov., sp. nov. and Methylonatrum kenyense gen. nov., sp. nov., methylotrophic gammaproteobacteria from hypersaline lakes","volume":"57","author":"Sorokin","year":"2007","journal-title":"Int. J. Sytem. Evolut. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Bhatt, P., Gangola, S., Udayanga, D., and Kumar, G. (2021). Synergistic Interaction of Methanotrophs and Methylotrophs in Regulating Methane Emission. Microbial Technology for Sustainable Environment, Springer.","DOI":"10.1007\/978-981-16-3840-4"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"107524","DOI":"10.1016\/j.fbio.2025.107524","article-title":"Ectoine from Sinobaca sp. regulated carotenoid accumulation in Dunaliella salina within the algal-bacterial co-culture system","volume":"72","author":"Luo","year":"2025","journal-title":"Food Biosci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1134\/S0003683819130015","article-title":"Engineering of Hydroxyectoine Production based on the Methylomicrobium alcaliphilum","volume":"55","author":"Mustakhimov","year":"2019","journal-title":"Appl. Biochem. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Kwon, S.J., Park, C.B., and Lee, P.C. (2024). Genomic Insights into the Role of cAMP in Carotenoid Biosynthesis: Enhancing \u03b2-Carotene Production in Escherichia coli via cyaA Deletion. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252312796"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"100251","DOI":"10.1016\/j.nxener.2025.100251","article-title":"Microbial methanotrophy: Methane capture to biomanufacturing of platform chemicals and fuels","volume":"8","author":"Madavi","year":"2025","journal-title":"Next Energy"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Ren, Y., Sun, H., Deng, J., Huang, J., and Chen, F. (2021). Carotenoid Production from Microalgae: Biosynthesis, Salinity Responses and Novel Biotechnologies. Mar. Drugs., 19.","DOI":"10.3390\/md19120713"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1099\/ijs.0.65317-0","article-title":"Classification of halo(alkali)philic and halo(alkali)tolerant methanotrophs provisionally assigned to the genera Methylomicrobium and Methylobacter and emended description of the genus Methylomicrobium","volume":"58","author":"Kalyuzhnaya","year":"2008","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_34","first-page":"114323","article-title":"Influence of design and operational parameters of a Taylor flow reactor on the bioconversion of methane to ectoines","volume":"12","author":"Monsalvo","year":"2024","journal-title":"J. Enrion. Chem. Engineer."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"104305","DOI":"10.1016\/j.eti.2025.104305","article-title":"Trisynergy of photosynthetic biogas upgrading, anaerobic digestate bioremediation, and pigment biosynthesis","volume":"39","author":"Komarysta","year":"2025","journal-title":"Environ. Technol. Innov."},{"key":"ref_36","unstructured":"Apha, A.W. (2012). Standard Methods for Examination of Water and Wastewater, American Public Health Association, Standard Methods. [22nd ed.]."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"131842","DOI":"10.1016\/j.biortech.2024.131842","article-title":"Syngas biological transformation into hydroxyectoine","volume":"417","author":"Lebrero","year":"2025","journal-title":"Bioresour. Technol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1093\/bioinformatics\/btv033","article-title":"MEGAHIT: An ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph","volume":"31","author":"Li","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nature13568","article-title":"Alterations of the human gut microbiome in liver cirrhosis","volume":"513","author":"Qin","year":"2014","journal-title":"Nature"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"766","DOI":"10.15252\/msb.20145645","article-title":"Potential of fecal microbiota for early-stage detection of colorectal cancer","volume":"10","author":"Zeller","year":"2014","journal-title":"Mol. Syst. Biol."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/24\/2\/81\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T05:14:11Z","timestamp":1771478051000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/24\/2\/81"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,17]]},"references-count":40,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["md24020081"],"URL":"https:\/\/doi.org\/10.3390\/md24020081","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,17]]}}}