{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:35:24Z","timestamp":1792805724799,"version":"4.5.0"},"reference-count":30,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T00:00:00Z","timestamp":1773532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Guangxi","award":["2025GXNSFBA069086"],"award-info":[{"award-number":["2025GXNSFBA069086"]}]},{"name":"Regional Innovation and Development set up by National Natural Science Foundation","award":["U24A20809"],"award-info":[{"award-number":["U24A20809"]}]},{"name":"Development Program of High-level Talent Team under the Qihuang Project of Guangxi University of Chinese Medicine","award":["202404"],"award-info":[{"award-number":["202404"]}],"id":[{"id":"https:\/\/ror.org\/024v0gx67","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Research Launching Fund Project from Guangxi University of Chinese Medicine Introduced the Doctoral","award":["2023BS057"],"award-info":[{"award-number":["2023BS057"]}],"id":[{"id":"https:\/\/ror.org\/024v0gx67","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Guangxi Youth Qihuang Scholar Training Project","award":["GXQH202412"],"award-info":[{"award-number":["GXQH202412"]}]},{"name":"Guangxi Special Support Program for Maritime Economic Talent Cultivation","award":["2025XHRC27"],"award-info":[{"award-number":["2025XHRC27"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Three new cyclic tetrapeptides (nectriatidels A-C, 1\u20133), two new curvularin analogs (6 and 7), and four known compounds (4 and 5, 8 and 9) were isolated from the marine-derived fungal-bacterial symbiont Aspergillus spelaeus GXIMD 04541\/Sphingomonas echinoides GXIMD 04532, which was obtained from Mauritia arabica in shallow coastal waters. Their structures were elucidated through NMR spectroscopy and HRESIMS, and their absolute configurations were determined by Marfey\u2019s method and quantum chemical calculations. Compounds 1\u20135 showed moderate amphotericin B (AmB)-potentiating activity against Candida albicans. Compounds 7 and 8 exhibited significant activities against Mycobacterium tuberculosis, with MIC values of 32 and 16 \u03bcg\/mL, respectively. Additionally, compounds 7 and 8 exhibited moderate cytotoxicity against human colorectal cancer cell lines DLD-1 and SW480, with IC50 values of 25~36 \u03bcM. Whole-genome sequencing of A. spelaeus revealed a 35.91 Mb assembly encoding 106 biosynthetic gene clusters (BGCs). antiSMASH analysis revealed that 79 of these BGCs (74.5%) displayed no significant similarity to known pathways in the MIBiG database, which is dominated by hybrid clusters, terpene, T1PKS, NRPS, and NRPS-like types. Genomic analysis identified the putative biosynthetic gene clusters for these metabolites and confirmed the fungal host as the predominant producer.<\/jats:p>","DOI":"10.3390\/md24030111","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T08:41:26Z","timestamp":1773650486000},"page":"111","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["New Cyclopeptides and Curvularins from Marine-Derived Fungal-Bacterial Symbiont Aspergillus spelaeus GXIMD 04541\/Sphingomonas echinoides GXIMD 04532"],"prefix":"10.3390","volume":"24","author":[{"given":"Fei-Hua","family":"Yao","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"},{"name":"Guangxi Key Laboratory of Marine Drugs, University Engineering Research Center of High-Efficient Utilization of Marine Traditional Chinese Medicine 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Guangxi University of Chinese Medicine, Nanning 530200, China"},{"name":"Guangxi Key Laboratory of Marine Drugs, University Engineering Research Center of High-Efficient Utilization of Marine Traditional Chinese Medicine Resources, Guangxi, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5519-7467","authenticated-orcid":false,"given":"Zhen-Zhou","family":"Tang","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"},{"name":"Guangxi Key Laboratory of Marine Drugs, University Engineering Research Center of High-Efficient Utilization of Marine Traditional Chinese Medicine Resources, Guangxi, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7037-7610","authenticated-orcid":false,"given":"Wei-Hui","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8327-3108","authenticated-orcid":false,"given":"Yong-Hong","family":"Liu","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"},{"name":"Guangxi Key Laboratory of Marine Drugs, University Engineering Research Center of High-Efficient Utilization of Marine Traditional Chinese Medicine Resources, Guangxi, Guangxi University of Chinese Medicine, Nanning 530200, 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