{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T01:32:50Z","timestamp":1784770370695,"version":"3.55.0"},"reference-count":52,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guangxi Key R&D Program","award":["GuikenongAB2506910042"],"award-info":[{"award-number":["GuikenongAB2506910042"]}]},{"name":"Guangxi Natural Science Foundation","award":["2026GXNSFAA00641308"],"award-info":[{"award-number":["2026GXNSFAA00641308"]}]},{"name":"National Key R&D Program","award":["2022YF2400700"],"award-info":[{"award-number":["2022YF2400700"]}]},{"name":"China Agriculture Research System Guangxi Innovation Team","award":["nycytxgxcxtd-2021-08"],"award-info":[{"award-number":["nycytxgxcxtd-2021-08"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Non-alcoholic fatty liver disease (NAFLD) is a primary metabolic disorder that threatens adolescent health globally, with no effective therapeutic agents currently available. Bellamya purificata is a traditional Chinese medicine categorized as \"medicinal food\", and polysaccharides are among its active components. However, its physicochemical structure remains poorly characterized, and no study has evaluated its effects on NAFLD. In this study, a homogeneous neutral polysaccharide, \u03b1-D-glucan (Mw = 6412.704 kDa), was isolated from B. purificata. The structure of the polysaccharide was characterized using monosaccharide composition analysis, methylation analysis, NMR spectroscopy, and scanning electron microscopy. The backbone structure of the polysaccharide comprises \u21924)-\u03b1-D-Glcp-(1\u2192 and \u21924,6)-\u03b1-D-Glcp-(1\u2192, with side chains of \u03b1-D-Glcp-(1\u2192 attached to the O-6 position of the 1\u21924,6)-\u03b1-D-Glcp-(1\u2192 sugar residues. Additionally, QSPS-1D effectively reduced weight gain, hepatic lipid accumulation (TC and TG), and inflammatory responses (tnf-\u03b1 and il-1\u03b2) in NAFLD zebrafish. Moreover, QSPS-1D alleviated dysbiosis by inhibiting harmful bacteria (e.g., Stenotrophomonas, Agrobacterium, and Chryseobacterium) and promoting beneficial microbiota (e.g., Rothia), which restored the Firmicutes-to-Bacteroidetes ratio. In parallel, it enhanced the expression of tight junction proteins (zo-1 and claudin-1), leading to the repair of the intestinal mucosal barrier. These findings suggest that B. purificata polysaccharides may be a potential functional food for early NAFLD intervention, with effects potentially associated with the modulation of the gut microbiota.<\/jats:p>","DOI":"10.3390\/md24050159","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T13:32:21Z","timestamp":1777555941000},"page":"159","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Structural Characterization of an \u03b1-D-glucan from Bellamya purificata and Its Protective Effects on Non-Alcoholic Fatty Liver Disease in Zebrafish"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8281-0327","authenticated-orcid":false,"given":"Xianhui","family":"Pan","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Guangxi University, Nanning 530003, China"},{"name":"Sean Key Laboratory of Comprehensive Exploitation and Utilization of Aquatic Germplasm Resources, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquaculture Genetic and Breeding and Healthy Aquaculture of Guangxi, Guangxi Academy of Fishery Sciences, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kangqi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Sean Key Laboratory of Comprehensive Exploitation and Utilization of Aquatic Germplasm Resources, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquaculture Genetic and Breeding and Healthy Aquaculture of Guangxi, Guangxi Academy of Fishery Sciences, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Meng","sequence":"additional","affiliation":[{"name":"Sean Key Laboratory of Comprehensive Exploitation and Utilization of Aquatic Germplasm Resources, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquaculture Genetic and Breeding and Healthy Aquaculture of Guangxi, Guangxi Academy of Fishery Sciences, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong","family":"Chen","sequence":"additional","affiliation":[{"name":"Sean Key Laboratory of Comprehensive Exploitation and Utilization of Aquatic Germplasm Resources, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquaculture Genetic and Breeding and Healthy Aquaculture of Guangxi, Guangxi Academy of Fishery Sciences, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuesong","family":"Du","sequence":"additional","affiliation":[{"name":"Sean Key Laboratory of Comprehensive Exploitation and Utilization of Aquatic Germplasm Resources, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquaculture Genetic and Breeding and Healthy Aquaculture of Guangxi, Guangxi Academy of Fishery Sciences, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqi","family":"Qin","sequence":"additional","affiliation":[{"name":"Sean Key Laboratory of Comprehensive Exploitation and Utilization of Aquatic Germplasm Resources, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquaculture Genetic and Breeding and Healthy Aquaculture of Guangxi, Guangxi Academy of Fishery Sciences, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Lin","sequence":"additional","affiliation":[{"name":"Sean Key Laboratory of Comprehensive Exploitation and Utilization of Aquatic Germplasm Resources, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquaculture Genetic and Breeding and Healthy Aquaculture of Guangxi, Guangxi Academy of Fishery Sciences, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingjun","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Guangxi University, Nanning 530003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"124495","DOI":"10.1016\/j.carbpol.2025.124495","article-title":"Structural characterisation of Cistanche tubulosa polysaccharides and their effects against non-alcoholic steatohepatitis via gut microbiota regulation","volume":"371","author":"Wang","year":"2025","journal-title":"Carbohydr. 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