{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T08:41:24Z","timestamp":1785400884961,"version":"3.56.0"},"reference-count":47,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Industry\u2013Education\u2013Research Collaborative Talent Cultivation Team","award":["2023YFD2400103"],"award-info":[{"award-number":["2023YFD2400103"]}]},{"name":"Nanping Science and Technology Expert Innovation & Entrepreneurship Competition Project","award":["N2023T026"],"award-info":[{"award-number":["N2023T026"]}]},{"name":"Nanping Resource Chemical Industry Science and Technology Innovation Joint Funding Project","award":["N2022Z002"],"award-info":[{"award-number":["N2022Z002"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82173731"],"award-info":[{"award-number":["82173731"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82103844"],"award-info":[{"award-number":["82103844"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["82173731"],"award-info":[{"award-number":["82173731"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["82103844"],"award-info":[{"award-number":["82103844"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Pyropia haitanensis polysaccharides have attracted growing attention for their diverse biological activities. In this study, we developed a synergistic extraction approach combining ultrasonic-assisted treatment and enzymatic hydrolysis using cellulase and pectinase. Response surface methodology (RSM) was applied to optimize the extraction conditions, which were determined as follows: 1.48% cellulase, 1.47% pectinase, 180 W ultrasonic power, and 65.9 \u00b0C temperature. Under these conditions, the polysaccharide yield reached 10.184 \u00b1 0.27%. The crude extract was then purified through sequential DEAE Sepharose FastFlow and Sephadex G-75 chromatography, resulting in the purified fraction PPHP3. Monosaccharide analysis revealed that galactose, glucose, and glucuronic acid constituted the primary components in a molar ratio of 98.3:0.46:1.24. This polysaccharide exhibited a weight-average molecular weight of 25.208 kDa, a sulfate content of 8.64 \u00b1 0.05%. In hypolipidemic assays using oleic acid-induced HepG2 cells, PPHP3 significantly reduced intracellular triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C), while simultaneously increasing HDL-C levels. These findings highlight the potential of P. haitanensis polysaccharides for hypolipidemic applications and establish a scientific foundation for their development in therapeutic and practical contexts.<\/jats:p>","DOI":"10.3390\/md24070241","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T15:40:09Z","timestamp":1783525209000},"page":"241","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Extraction and Purification of Polysaccharides from Thermotolerant Pyropia haitanensis Strain SW-81 and Its Hypolipidemic Effects on Oleic Acid-Induced Lipid Accumulation in HepG2 Cells"],"prefix":"10.3390","volume":"24","author":[{"given":"Jiawei","family":"Zhong","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongchang","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jogeir","family":"Toppe","sequence":"additional","affiliation":[{"name":"Food and Agriculture Organization of the United Nations (FAO), 00153 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiyue","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menghan","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quancai","family":"Sun","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Peng","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1710-8582","authenticated-orcid":false,"given":"Wenhui","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Putuo Sub-Center of International Joint Research Center for Marine Biological Sciences, Zhoushan 316104, China"},{"name":"Marine Biomedical Science and Technology Innovation Platform of Lin-Gang Special Area, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5942-6208","authenticated-orcid":false,"given":"Wanqiang","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Marine Biomedical Science and Technology Innovation Platform of Lin-Gang Special Area, Shanghai 201306, China"},{"name":"International Research Center for Food and Health, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xichang","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2879","DOI":"10.1161\/STROKEAHA.125.051754","article-title":"Aging and Sex Modify the Risk of Carotid Plaque Thrombosis Related to Dyslipidemic Profile","volume":"56","author":"Servadei","year":"2025","journal-title":"Stroke"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1001\/jamapediatrics.2021.2046","article-title":"Low-Density Lipoprotein Cholesterol Trajectories and Prevalence of High Low-Density Lipoprotein Cholesterol Consistent with Heterozygous Familial Hypercholesterolemia in US Children","volume":"175","author":"Zhang","year":"2021","journal-title":"JAMA Pediatr."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Kwon, Y.-J., Lee, S., Lee, H.S., and Lee, J.-W. 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