{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:04:12Z","timestamp":1785416652442,"version":"3.56.0"},"reference-count":28,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82104061"],"award-info":[{"award-number":["82104061"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["82104061"],"award-info":[{"award-number":["82104061"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013806","name":"Talent Youth Teacher Program in Shenyang Pharmaceutical University","doi-asserted-by":"publisher","award":["YQ202203"],"award-info":[{"award-number":["YQ202203"]}],"id":[{"id":"10.13039\/501100013806","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Educational Commission of Liaoning Province of China","award":["JYTQN2023333"],"award-info":[{"award-number":["JYTQN2023333"]}]},{"DOI":"10.13039\/501100010086","name":"Liaoning Province Education Administration Foundation","doi-asserted-by":"publisher","award":["LJ212510163006"],"award-info":[{"award-number":["LJ212510163006"]}],"id":[{"id":"10.13039\/501100010086","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Opening Project of Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry","award":["GXMNPCBC-2023-01"],"award-info":[{"award-number":["GXMNPCBC-2023-01"]}]},{"name":"Youth Innovation Research Team Project of Guangxi University of Chinese Medicine","award":["2023TD003"],"award-info":[{"award-number":["2023TD003"]}]},{"award":["2023TD003"],"award-info":[{"award-number":["2023TD003"]}],"id":[{"id":"https:\/\/ror.org\/024v0gx67","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Four novel alkaloids, including three \u03b3-lactam alkaloids (1\u20133) and one diketopiperazine (4), along with eight previously known compounds (5\u201312), were isolated from the marine-derived fungus Aspergillus fumigatus SYPHU504. Their structures, including the tentative stereochemical assignments of the side-chain double bonds in 2 and 3, were elucidated through comprehensive spectroscopic analysis and in comparison with the literature\u2019s data. All isolated compounds were evaluated for their anti-leukaemic activities against human leukaemia cell lines K562 and RS4;11 using the MTT assay. Compounds 4, 6, 7, and 9\u201312 exhibited notable cytotoxic activities against both RS4;11 and K562 cell lines, with IC50 values ranging from 5.02 \u00b1 2.33 to 31.85 \u00b1 0.50 \u03bcM. The results of Western blotting and Annexin V-FITC\/PI staining elucidated that compounds 4, 7, and 10 could induce apoptosis in both RS4;11 cells and K562 cells.<\/jats:p>","DOI":"10.3390\/md24070247","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:55:59Z","timestamp":1784249759000},"page":"247","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and Characterisation of Alkaloids from Marine-Derived Aspergillus fumigatus SYPHU504 with Antiproliferative Activity"],"prefix":"10.3390","volume":"24","author":[{"given":"Xuelei","family":"Zhang","sequence":"first","affiliation":[{"name":"Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingshu","family":"Yu","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0097-0723","authenticated-orcid":false,"given":"Kai","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Drugs and Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8327-3108","authenticated-orcid":false,"given":"Yonghong","family":"Liu","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2579-2123","authenticated-orcid":false,"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Clinical Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8417-1784","authenticated-orcid":false,"given":"Jiao","family":"Xiao","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China"},{"name":"School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1016\/j.bcp.2009.05.027","article-title":"Models for Prevention and Treatment of Cancer: Problems vs. Promises","volume":"78","author":"Aggarwal","year":"2009","journal-title":"Biochem. 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