{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:08:22Z","timestamp":1785413302752,"version":"3.56.0"},"reference-count":125,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mar. Drugs","Marine Drugs"],"abstract":"<jats:p>The increasing interest in natural versus synthetic additives is a driving force for food industry innovation to develop alternative solutions for food safety and quality. Brown algae, described as containing potential antimicrobial and antioxidant compounds, are promising alternative sources. However, optimized food-grade extracts require preserving their bioactivity. In this study, the effects of seasonality and interannual variation, together with non-thermal high-hydrostatic-pressure (HHP) pretreatment, on the extraction of antioxidant and antimicrobial compounds from the macroalga Ericaria selaginoides, collected from the northwest of Spain between November 2021 and September 2023, were assessed. The HHP pretreatment of fresh algae did not significantly change the yield of the crude extracts and only slightly improved the antimicrobial activity of the extracts against L. monocytogenes, S. aureus and B. cereus, without significantly diminishing the antioxidant activity until 600 MPa for 5 min, the total polyphenol content (TPC), and the chlorophyll A content. Interannual and seasonal variations significantly influenced pigments and protein, carbohydrate and polyphenol contents, together with the antioxidant and antimicrobial activities, of the extracts. By NMR, phloroglucinol was identified as the major secondary metabolite, together with mannitol, alanine and a mixture of meroditerpenoids, which may contribute to the reported activities. The overall results demonstrated the role of environmental factors in driving seasonal and interannual changes in macroalgal metabolism, significantly influencing the bioactive properties of extracts, while the effect of HHP pretreatment was minimal.<\/jats:p>","DOI":"10.3390\/md24080257","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T05:49:27Z","timestamp":1785131367000},"page":"257","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Seasonality: The Driving Force Behind the Antimicrobial and Antioxidant Activities and Biochemical Composition of Ericaria selaginoides Extracts, with Minimal Effect of High Hydrostatic Pressure Pretreatment"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0272-3734","authenticated-orcid":false,"given":"Sunuram","family":"Ray","sequence":"first","affiliation":[{"name":"Food Safety and Functionality Program, Institute of Agrifood Research and Technology (IRTA), 17121 Monells, Girona, Spain"},{"name":"Institute for Integrated Studies on the Sundarbans and Coastal Ecosystems (IISSCE), Khulna University, Khulna 9208, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Hortos","sequence":"additional","affiliation":[{"name":"Food Safety and Functionality Program, Institute of Agrifood Research and Technology (IRTA), 17121 Monells, Girona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Casal-Silva","sequence":"additional","affiliation":[{"name":"Institute of Natural Products and Agrobiology (IPNA-CSIC), 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9112-6877","authenticated-orcid":false,"given":"Mercedes","family":"Cueto","sequence":"additional","affiliation":[{"name":"Institute of Natural Products and Agrobiology (IPNA-CSIC), 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1110-9445","authenticated-orcid":false,"given":"Teresa","family":"Aymerich","sequence":"additional","affiliation":[{"name":"Food Safety and Functionality Program, Institute of Agrifood Research and Technology (IRTA), 17121 Monells, Girona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1016\/j.foodchem.2012.11.081","article-title":"Valuable compounds in macroalgae extracts","volume":"138","author":"Andrade","year":"2013","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1002\/elsc.201400191","article-title":"Algae as production systems of bioactive compounds","volume":"15","author":"Michalak","year":"2015","journal-title":"Eng. Life Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"112460","DOI":"10.1016\/j.fct.2021.112460","article-title":"Brown marine algae Gongolaria baccata extract protects Caco-2 cells from oxidative stress induced by tert-butyl hydroperoxide","volume":"156","author":"Ares","year":"2021","journal-title":"Food Chem. Toxicol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Shannon, E., and Abu-Ghannam, N. (2016). Antibacterial Derivatives of Marine Algae: An Overview of Pharmacological Mechanisms and Applications. Mar. Drugs, 14.","DOI":"10.3390\/md14040081"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kumar, Y., Tarafdar, A., Kumar, D., Saravanan, C., Badgujar, P.C., Pharande, A., Pareek, S., and Fawole, O.A. (2022). Polyphenols of edible macroalgae: Estimation of in vitro bio-accessibility and cytotoxicity, quantification by LC-MS\/MS and potential utilization as an antimicrobial and functional food ingredient. Antioxidants, 11.","DOI":"10.3390\/antiox11050993"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Biris-Dorhoi, E.-S., Michiu, D., Pop, C.R., Rotar, A.M., Tofana, M., Pop, O.L., Socaci, S.A., and Farcas, A.C. (2020). Macroalgae\u2014A sustainable source of chemical compounds with biological activities. Nutrients, 12.","DOI":"10.3390\/nu12103085"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1080\/10799893.2020.1850787","article-title":"Aloperine protects human retinal pigment epithelial cells against hydrogen peroxide\u2013induced oxidative stress and apoptosis through activation of Nrf2\/HO-1 pathway","volume":"42","author":"Zhang","year":"2022","journal-title":"J. Recept. Signal Transduct."},{"key":"ref_8","unstructured":"Lund, B.M., Baird-Parker, T.C., and Gould, G.W. (2000). Microbiological Safety and Quality of Food, Springer Science & Business Media."},{"key":"ref_9","unstructured":"UNEP (2024). Food Waste Index Report 2024. Think Eat Save: Tracking Progress to Halve Global Food Waste, UNEP."},{"key":"ref_10","unstructured":"FAO Sustainable Development Goals, Food and Agriculture Organization of the United Nations (FAO). Available online: https:\/\/www.fao.org\/sustainable-development-goals\/indicators\/1231\/en\/."},{"key":"ref_11","first-page":"8505E","article-title":"2023 Crisis Preparedness Training: Annual Report","volume":"20","author":"Upton","year":"2023","journal-title":"EFSA Support. Publ."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Mendonca, A., Thomas-Popo, E., and Gordon, A. (2020). Microbiological considerations in food safety and quality systems implementation. Food Safety and Quality Systems in Developing Countries, Elsevier.","DOI":"10.1016\/B978-0-12-814272-1.00005-X"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"EFSA (2024). The European Union one health 2023 zoonoses report. EFSA J., 22, e9106.","DOI":"10.2903\/j.efsa.2024.p221202"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Thakur, M., Asrani, R.K., and Patial, V. (2018). Listeria monocytogenes: A food-borne pathogen. Foodborne Diseases, Elsevier.","DOI":"10.1016\/B978-0-12-811444-5.00006-3"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"EFSA (2025). The European Union One Health 2024 Zoonoses Report. EFSA J., 23, e9759.","DOI":"10.2903\/j.efsa.2025.p231201"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"P\u00e9rez, M.J., Falqu\u00e9, E., and Dom\u00ednguez, H. (2016). Antimicrobial action of compounds from marine seaweed. Mar. Drugs, 14.","DOI":"10.3390\/md14030052"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Gaur, R., Singh, A., and Tripathi, A. (2019). Microbial environment of food. Food Safety and Human Health, Elsevier.","DOI":"10.1016\/B978-0-12-816333-7.00008-4"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1111\/1541-4337.12241","article-title":"Mechanisms of oxidative processes in meat and toxicity induced by postprandial degradation products: A review","volume":"16","author":"Papuc","year":"2017","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Franco, R., Navarro, G., and Martinez-Pinilla, E. (2019). Antioxidants versus Food Antioxidant Additives and Food Preservatives. Antioxidants, 8.","DOI":"10.3390\/antiox8110542"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"9093","DOI":"10.1021\/acsomega.9b03687","article-title":"Polyphenols from brown seaweeds as a potential antimicrobial agent in animal feeds","volume":"5","author":"Ford","year":"2020","journal-title":"ACS Omega"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.foodres.2010.12.030","article-title":"Marine food-derived functional ingredients as potential antioxidants in the food industry: An overview","volume":"44","author":"Ngo","year":"2011","journal-title":"Food Res. Int."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Brien, R.O., Hayes, M., Sheldrake, G., Tiwari, B., and Walsh, P. (2022). Macroalgal proteins: A review. Foods, 11.","DOI":"10.3390\/foods11040571"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.cofs.2015.08.004","article-title":"Natural food pigments and colorants","volume":"7","author":"Delia","year":"2016","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3033","DOI":"10.1111\/1750-3841.15782","article-title":"Effect of emulsifiers on the discoloration of chlorophyll and their potential for use in green beverages","volume":"86","author":"Yasuda","year":"2021","journal-title":"J. Food Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s11010-009-0145-y","article-title":"Comparative effects of \u03b2-carotene and fucoxanthin on retinol deficiency induced oxidative stress in rats","volume":"331","author":"Sangeetha","year":"2009","journal-title":"Mol. Cell. Biochem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/j.foodres.2016.09.023","article-title":"Characterization of dietary fucoxanthin from Himanthalia elongata brown seaweed","volume":"99","author":"Rajauria","year":"2017","journal-title":"Food Res. Int."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1007\/s10811-023-02923-2","article-title":"Antimicrobial potential of Ericaria selaginoides extracts against Listeria monocytogenes in \u201cmat\u00f3\u201d, a Catalan fresh cheese","volume":"35","author":"Aymerich","year":"2023","journal-title":"J. Appl. Phycol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Garcia-Vaquero, M., Rajauria, G., Miranda, M., Sweeney, T., Lopez-Alonso, M., and O\u2019Doherty, J. (2021). Seasonal variation of the proximate composition, mineral content, fatty acid profiles and other phytochemical constituents of selected brown macroalgae. Mar. Drugs, 19.","DOI":"10.3390\/md19040204"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Kraan, S. (2012). Algal polysaccharides, novel applications and outlook. Carbohydrates\u2014Comprehensive Studies on Glycobiology and Glycotechnology, IntechOpen.","DOI":"10.5772\/51572"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3123","DOI":"10.1016\/S0040-4039(00)77425-9","article-title":"Bifurcarenone, an inhibitor of mitotic cell division from the brown alga Bifurcaria galapagensis","volume":"21","author":"Sun","year":"1980","journal-title":"Tetrahedron Lett."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"102190","DOI":"10.1016\/j.algal.2021.102190","article-title":"Acclimation potential and biochemical response of four temperate macroalgae to light and future seasonal temperature scenarios","volume":"54","author":"Schmid","year":"2021","journal-title":"Algal Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"110942","DOI":"10.1016\/j.lwt.2021.110942","article-title":"Antioxidant and anti-norovirus properties of aqueous acetic acid macromolecular extracts of edible brown macroalgae","volume":"141","author":"Kuda","year":"2021","journal-title":"LWT Food Sci. Technol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"22241","DOI":"10.1038\/s41598-023-47274-7","article-title":"Temporal patterns of fucoxanthin in four species of European marine brown macroalgae","volume":"13","author":"Cunningham","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1007\/s10811-016-0983-4","article-title":"Optimisation of fucoxanthin extraction from Irish seaweeds by response surface methodology","volume":"29","author":"Shannon","year":"2017","journal-title":"J. Appl. Phycol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Sosa-Hern\u00e1ndez, J.E., Escobedo-Avellaneda, Z., Iqbal, H.M., and Welti-Chanes, J. (2018). State-of-the-art extraction methodologies for bioactive compounds from algal biome to meet bio-economy challenges and opportunities. Molecules, 23.","DOI":"10.3390\/molecules23112953"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1089\/jmf.2006.099","article-title":"The antioxidant properties of brown seaweed (Sargassum siliquastrum) extracts","volume":"10","author":"Cho","year":"2007","journal-title":"J. Med. Food"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.1016\/j.foodchem.2012.10.078","article-title":"Phenolic compounds and antioxidant activities of selected species of seaweeds from Danish coast","volume":"138","author":"Farvin","year":"2013","journal-title":"Food Chem."},{"key":"ref_38","unstructured":"European Union (2009). Directive 2009\/28\/EC of the European Parliament and of the Council of 23 April 2009 on the promotion of the use of energy from renewable sources and amending and subsequently repealing Directives 2001\/77\/EC and 2003\/30\/EC. Off. J. Eur. Union, 5, 2009."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Chemat, F., Abert Vian, M., Ravi, H.K., Khadhraoui, B., Hilali, S., Perino, S., and Fabiano Tixier, A.S. (2019). Review of alternative solvents for green extraction of food and natural products: Panorama, principles, applications and prospects. Molecules, 24.","DOI":"10.3390\/molecules24163007"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"101810","DOI":"10.1016\/j.biteb.2024.101810","article-title":"Recovery of bioactive complex from brown algae by alternative extraction approaches","volume":"26","author":"Bogolitsyn","year":"2024","journal-title":"Bioresour. Technol. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.tifs.2013.07.001","article-title":"Advances in the extraction of natural ingredients by high pressure extraction technology","volume":"33","author":"Huang","year":"2013","journal-title":"Trends Food Sci. Technol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1515\/pjfns-2015-0011","article-title":"Effect of high hydrostatic pressure processing strategies on retention of antioxidant phenolic bioactives in foods and beverages\u2014A review","volume":"66","year":"2016","journal-title":"Pol. J. Food Nutr. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4667","DOI":"10.1021\/jf400819p","article-title":"Application of Novel Extraction Technologies for Bioactives from Marine Algae","volume":"61","author":"Kadam","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"20175","DOI":"10.1016\/j.carbpol.2022.120175","article-title":"Structural and functional properties of alginate obtained by means of high hydrostatic pressure-assisted extraction","volume":"299","author":"Bojorges","year":"2023","journal-title":"Carbohydr. Polym."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.3390\/molecules25082005","article-title":"Extraction of protein from four different seaweeds using three different physical pre-treatment strategies","volume":"25","author":"Meaney","year":"2020","journal-title":"Molecules"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Morales-Sierra, S., Luis, J.C., Jim\u00e9nez-Arias, D., Rancel-Rodr\u00edguez, N.M., Coego, A., Rodriguez, P.L., Cueto, M., and Borges, A.A. (2023). Biostimulant activity of Galaxaura rugosa seaweed extracts against water deficit stress in tomato seedlings involves activation of ABA signaling. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1251442"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Ahmed, H.S., Abouzeid, H., Mansour, M.A., Owis, A.I., Amin, E., Darwish, H.W., Alanazi, A.S., Naguib, I.A., Afifi, N., and Ahmed, H.S. (2023). Antioxidant and Anti-Aging Phytoconstituents from Faucaria tuberculosa: In Vitro and In Silico Studies. Molecules, 28.","DOI":"10.3390\/molecules28196895"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.1080\/14786419.2019.1574784","article-title":"NMR-based metabolic study of leaves of three species of Actinidia with different degrees of susceptibility to Pseudomonas syringae pv. actinidiae","volume":"34","author":"Sciubba","year":"2020","journal-title":"Nat. Prod. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1021\/np300833y","article-title":"Antioxidant and anti-inflammatory meroterpenoids from the brown alga Cystoseira usneoides","volume":"76","author":"Zbakh","year":"2013","journal-title":"J. Nat. Prod."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1016\/S0031-9422(00)82274-8","article-title":"Tetraprenyltoluquinols from the brown alga Cystoseira stricta","volume":"26","author":"Amico","year":"1987","journal-title":"Phytochemistry"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1016\/0031-9422(95)00750-4","article-title":"Meroditerpenes from the brown alga Cystoseira amentacea var. stricta collected off the French mediterranean coast","volume":"41","author":"Valls","year":"1996","journal-title":"Phytochemistry"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1007\/s11947-018-2229-8","article-title":"The dietary fiber profile, total polyphenol content, functionality of Silvetia compressa and Ecklonia arborea, and modifications induced by high hydrostatic pressure treatments","volume":"12","year":"2019","journal-title":"Food Bioprocess Technol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jfoodeng.2019.02.014","article-title":"Effects of high hydrostatic pressure and polysaccharidases on the extraction of antioxidant compounds from red macroalgae, Palmaria palmata and Solieria chordalis","volume":"252","author":"Suwal","year":"2019","journal-title":"J. Food Eng."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"12977","DOI":"10.1111\/jfpp.12977","article-title":"Bioactive polysaccharides extracts from Sargassum muticum by high hydrostatic pressure","volume":"41","author":"Rodrigues","year":"2017","journal-title":"J. Food Process. Preserv."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.enzmictec.2009.03.004","article-title":"High hydrostatic pressure increased stability and activity of immobilized lipase in hexane","volume":"45","author":"Eisenmenger","year":"2009","journal-title":"Enzym. Microb. Technol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.ifset.2004.02.002","article-title":"On the possibility of using high pressure treatment to modify physico-chemical properties of dietary fibre in white cabbage (Brassica oleracea var. capitata)","volume":"5","author":"Wennberg","year":"2004","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"128259","DOI":"10.1016\/j.foodchem.2020.128259","article-title":"Ultrasound-assisted preparation of anise extract nanoemulsion and its bioactivity against different pathogenic bacteria","volume":"341","author":"Ghazy","year":"2021","journal-title":"Food Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.ultsonch.2014.10.007","article-title":"Optimization of ultrasound assisted extraction of bioactive components from brown seaweed Ascophyllum nodosum using response surface methodology","volume":"23","author":"Kadam","year":"2015","journal-title":"Ultrason. Sonochem."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Ayrapetyan, O.N., Obluchinskaya, E.D., Zhurishkina, E.V., Skorik, Y.A., Lebedev, D.V., Kulminskaya, A.A., and Lapina, I.M. (2021). Antibacterial properties of fucoidans from the brown algae Fucus vesiculosus L. of the barents sea. Biology, 10.","DOI":"10.3390\/biology10010067"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Smu\u0142ek, W., and Kaczorek, E. (2022). Factors influencing the bioavailability of organic molecules to bacterial cells\u2014a mini-review. Molecules, 27.","DOI":"10.3390\/molecules27196579"},{"key":"ref_61","first-page":"129","article-title":"Phlorotannins, brown algal polyphenols","volume":"4","author":"Ragan","year":"1986","journal-title":"Prog. Phycol. Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"101938","DOI":"10.1016\/j.algal.2020.101938","article-title":"Preservation of five edible seaweeds by high pressure processing: Effect on microbiota, shelf life, colour, texture and antioxidant capacity","volume":"49","author":"Picon","year":"2020","journal-title":"Algal Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1042\/bj2920310","article-title":"Mechanism of beta-carotene degradation","volume":"292","author":"Mordi","year":"1993","journal-title":"Biochem. J."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.biotechadv.2011.05.016","article-title":"Algal chemodiversity and bioactivity: Sources of natural variability and implications for commercial application","volume":"29","author":"Stengel","year":"2011","journal-title":"Biotechnol. Adv."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1515\/BOT.2006.005","article-title":"Intra-thallus phlorotannin content and antioxidant activity in Phaeophyceae of temperate waters","volume":"49","author":"Connan","year":"2006","journal-title":"Bot. Mar."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1007\/s00343-019-7046-z","article-title":"Antioxidative and antimicrobial activities of intertidal seaweeds and possible effects of abiotic factors on these bioactivities","volume":"36","author":"Xu","year":"2018","journal-title":"J. Oceanol. Limnol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1515\/BOT.2004.058","article-title":"Phenolic contents of two brown algae, Turbinaria ornata and Sargassum mangarevense on Tahiti (French Polynesia): Interspecific, ontogenic and spatio-temporal variations","volume":"47","author":"Stiger","year":"2004","journal-title":"Bot. Mar."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.marenvres.2015.11.014","article-title":"Seasonal bio-chemical and photophysiological responses in the intertidal macroalga Cystoseira tamariscifolia (Ochrophyta)","volume":"115","author":"Bouzon","year":"2016","journal-title":"Mar. Environ. Res."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"21044","DOI":"10.1038\/s41598-024-71961-8","article-title":"Assessment of the antibacterial and antioxidant activities of seaweed-derived extracts","volume":"14","author":"Hejna","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Kamal, M., Abdel-Raouf, N., Alwutayd, K., AbdElgawad, H., Abdelhameed, M.S., Hammouda, O., and Elsayed, K.N. (2023). Seasonal changes in the biochemical composition of dominant macroalgal species along the Egyptian Red Sea shore. Biology, 12.","DOI":"10.3390\/biology12030411"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.jembe.2004.07.016","article-title":"Seasonal variation in antifouling activity of crude extracts of the brown alga Bifurcaria bifurcata (Cystoseiraceae) against cyprids of Balanus amphitrite and the marine bacteria Cobetia marina and Pseudoalteromonas haloplanktis","volume":"313","author":"Culioli","year":"2004","journal-title":"J. Exp. Mar. Biol. Ecol."},{"key":"ref_72","first-page":"111","article-title":"Seasonal variation of antibacterial activity of the brown alga Padina pavonica (l) Thivy collected from northern coast of Tunisia","volume":"37","author":"Ali","year":"2010","journal-title":"INSTM Bull. Mar. Freshw. Sci."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1051\/alr\/2014009","article-title":"Seasonal antibacterial activity of two red seaweeds, Palmaria palmata and Grateloupia turuturu, on European abalone pathogen Vibrio harveyi","volume":"27","author":"Rajauria","year":"2014","journal-title":"Aquat. Living Resour."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1080\/09670260802578542","article-title":"Seasonal variation of polyphenolics in Ascophyllum nodosum (Phaeophyceae)","volume":"44","author":"Parys","year":"2009","journal-title":"Eur. J. Phycol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"923","DOI":"10.2331\/fishsci.62.923","article-title":"Antioxidant activity of phlorotannins isolated from the brown alga Eisenia bicyclis","volume":"62","author":"Nakamura","year":"1996","journal-title":"Fish. Sci."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Almeida, B., Barroso, S., Ferreira, A.S., Ad\u00e3o, P., Mendes, S., and Gil, M.M. (2021). Seasonal evaluation of phlorotannin-enriched extracts from brown macroalgae Fucus spiralis. Molecules, 26.","DOI":"10.3390\/molecules26144287"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Celis-Pla, P.S., Trabal, A., Navarrete, C., Troncoso, M., Moenne, F., Z\u00fa\u00f1iga, A., Figueroa, F.L., and S\u00e1ez, C.A. (2022). Daily changes on seasonal ecophysiological responses of the intertidal brown macroalga Lessonia spicata: Implications of climate change. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.941061"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.ejar.2019.05.001","article-title":"Growth attributes and biochemical composition of Padina pavonica (L.) from the Red Sea, in response to seasonal alterations of Tabuk, Saudi Arabia","volume":"45","author":"Ansari","year":"2019","journal-title":"Egypt. J. Aquat. Res."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Paiva, L., Lima, E., Neto, A.I., and Baptista, J. (2018). Seasonal variability of the biochemical composition and antioxidant properties of Fucus spiralis at two Azorean Islands. Mar. Drugs, 16.","DOI":"10.3390\/md16080248"},{"key":"ref_80","first-page":"423","article-title":"Effect of physicochemical parameters of seawater on antioxidant capacity in green, brown, and red macroalgae from the Persian Gulf","volume":"22","author":"Farasat","year":"2023","journal-title":"Iran. J. Fish. Sci."},{"key":"ref_81","first-page":"123","article-title":"Measurement of antioxidant activities and phenolic and flavonoid contents of the brown seaweed Sargassum horneri: Comparison of supercritical CO2 and various solvent extractions","volume":"18","author":"Yin","year":"2015","journal-title":"Fish. Aquat. Sci."},{"key":"ref_82","first-page":"1","article-title":"NMR use to quantify phlorotannins: The case of Cystoseira tamariscifolia, a phloroglucinol-producing brown macroalga in Brittany (France)","volume":"135","author":"Kervarec","year":"2015","journal-title":"Talanta"},{"key":"ref_83","first-page":"2947","article-title":"Antioxidant potential of phloroglucinol; An in vitro approach","volume":"9","author":"Archana","year":"2018","journal-title":"Int. J. Pharm. Sci. Res."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"4817","DOI":"10.1021\/acs.jafc.2c00532","article-title":"Phloroglucinol and its derivatives: Antimicrobial properties toward microbial pathogens","volume":"70","author":"Khan","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1080\/10498850.2013.851757","article-title":"Damage to the membrane permeability and cell death of Vibrio parahaemolyticus caused by phlorotannins with low molecular weight from Sargassum thunbergii","volume":"25","author":"Wei","year":"2016","journal-title":"J. Aquat. Food Prod. Technol."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Chouh, A., Nouadri, T., Catarino, M.D., Silva, A.M., and Cardoso, S.M. (2022). Phlorotannins of the brown algae Sargassum vulgare from the Mediterranean Sea coast. Antioxidants, 11.","DOI":"10.3390\/antiox11061055"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"J\u00e9gou, C., Connan, S., Bihannic, I., C\u00e9rantola, S., Gu\u00e9rard, F., and Stiger-Pouvreau, V. (2021). Phlorotannin and pigment content of native canopy-forming Sargassaceae species living in intertidal rockpools in Brittany (France): Any relationship with their vertical distribution and phenology?. Mar. Drugs, 19.","DOI":"10.3390\/md19090504"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1007\/s11802-019-4019-y","article-title":"Fucoxanthin isolated from Undaria pinnatifida can interact with Escherichia coli and lactobacilli in the intestine and inhibit the growth of pathogenic bacteria","volume":"18","author":"Liu","year":"2019","journal-title":"J. Ocean Univ. China"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Louren\u00e7o-Lopes, C., Fraga-Corral, M., Soria-Lopez, A., Nu\u00f1es-Estevez, B., Barral-Martinez, M., Silva, A., Li, N., Liu, C., Simal-Gandara, J., and Prieto, M.A. (2022). Fucoxanthin\u2019s optimization from Undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies. Antioxidants, 11.","DOI":"10.3390\/antiox11071296"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"C104","DOI":"10.1111\/j.1750-3841.2010.01915.x","article-title":"Comparative antioxidant activity of edible Japanese brown seaweeds","volume":"76","author":"Airanthi","year":"2011","journal-title":"J. Food Sci."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1590\/S0102-695X2011005000010","article-title":"Antioxidant activity and possible bioactive components in hydrophilic and lipophilic fractions from the seaweed Halimeda incrassata","volume":"21","author":"Novoa","year":"2011","journal-title":"Rev. Bras. Farmacogn."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.fitote.2011.10.016","article-title":"Enzyme-assistant extraction (EAE) of bioactive components: A useful approach for recovery of industrially important metabolites from seaweeds: A review","volume":"83","author":"Wijesinghe","year":"2012","journal-title":"Fitoterapia"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.ifset.2016.06.004","article-title":"Comparison of extraction methods for selected carotenoids from macroalgae and the assessment of their seasonal\/spatial variation","volume":"37","author":"Heffernan","year":"2016","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.ejar.2017.04.001","article-title":"Seasonal variation in the growth responses of some chlorophytic algal flora of the Red Sea","volume":"43","author":"Ansari","year":"2017","journal-title":"Egypt. J. Aquat. Res."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1111\/j.1529-8817.2007.00416.x","article-title":"Seasonal growth, erosion rates, and nitrogen and photosynthetic ecophysiology of Undaria pinnatifida (Heterokontophyta) in southern New Zealand","volume":"43","author":"Dean","year":"2007","journal-title":"J. Phycol."},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Ismail, M.M., El Zokm, G.M., and Miranda Lopez, J.M. (2023). Nutritional, bioactive compounds content, and antioxidant activity of brown seaweeds from the Red Sea. Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1210934"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.1016\/j.foodchem.2012.11.026","article-title":"In vitro antioxidant properties of crude extracts and compounds from brown algae","volume":"138","author":"Balboa","year":"2013","journal-title":"Food Chem."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"012057","DOI":"10.1088\/1755-1315\/1033\/1\/012057","article-title":"Pigments constituents, phenolic content and anti-oxidant activity of brown seaweed Sargassum sp","volume":"1003","author":"Sanger","year":"2022","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1515\/bmc-2022-0003","article-title":"Seaweeds\u2019 pigments and phenolic compounds with antimicrobial potential","volume":"13","author":"Gomes","year":"2022","journal-title":"Biomol. Concepts"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1134\/S0003683814010037","article-title":"Biological activity of lipids and photosynthetic pigments of Sargassum pallidum C. Agardh","volume":"50","author":"Gerasimenko","year":"2014","journal-title":"Appl. Biochem. Microbiol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.jff.2015.06.026","article-title":"Characterization of antibacterial activity from protein hydrolysates of the macroalga Saccharina longicruris and identification of peptides implied in bioactivity","volume":"17","author":"Beaulieu","year":"2015","journal-title":"J. Funct. Foods"},{"key":"ref_102","unstructured":"Dahl, L. (2018). Composition of Laminaria digitata Biomass During a Potential Harvesting Season, Chalmers University of Technology."},{"key":"ref_103","unstructured":"Marante, F.J.T. (2016, January 20\u201322). Antioxidant activity of metabolites from the brown algae Halopteris scoparia (=Stypocaulon scoparium). Proceedings of the V Simposio Internacional de Ciencias del Mar, Alicante, Spain."},{"key":"ref_104","doi-asserted-by":"crossref","unstructured":"Stiger-Pouvreau, V., Jegou, C., Cerantola, S., Gu\u00e9rard, F., and Le Lann, K. (2014). Phlorotannins in Sargassaceae species from Brittany (France): Interesting molecules for ecophysiological and valorisation purposes. Advances in Botanical Research, Elsevier.","DOI":"10.1016\/B978-0-12-408062-1.00013-5"},{"key":"ref_105","unstructured":"J\u00e9gou, C. (2011). \u00c9tude du Genre Cystoseira des C\u00f4tes Bretonnes: Taxinomie, \u00c9cologie et Caract\u00e9risation de Substances Naturelles. [Ph.D. Thesis, Universit\u00e9 de Bretagne Occidentale]."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1021\/acs.jnatprod.5b01067","article-title":"Cystoseira usneoides: A brown alga rich in antioxidant and anti-inflammatory meroditerpenoids","volume":"79","author":"Ortega","year":"2016","journal-title":"J. Nat. Prod."},{"key":"ref_107","unstructured":"WeatherSpark (2025, February 04). Historical Weather During 2022 in Santander, Spain. WeatherSpark. Available online: https:\/\/weatherspark.com\/h\/y\/37498\/2022\/Historical-Weather-during-2022-in-Santander-Spain#Figures-Temperature."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"21408","DOI":"10.1038\/s41598-020-78526-5","article-title":"Heatwaves during low tide are critical for the physiological performance of intertidal macroalgae under global warming scenarios","volume":"10","author":"Troncoso","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_109","unstructured":"C\u00e1liz, A.C., Fern\u00e1ndez, A.N., de Pedro, R.S., Flores-Moya, A., and Ba\u00f1ares-Espa\u00f1a, E. (2019). Physiological responses of adults and juveniles of Cystoseira tamariscifolia to projected warming scenarios along Alboran sea populations. Proceedings of the II International Congress of Young Marine Researchers. Book of Abstracts, Malaga, Spain, 1\u20134 October 2019, University of M\u00e1laga."},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Falace, A., Marletta, G., Savonitto, G., Candotto Carniel, F., Srijemsi, M., Bevilacqua, S., Tretiach, M., and Alongi, G. (2021). Is the South-Mediterranean canopy-forming Ericaria giacconei (=Cystoseira hyblaea) a loser from ocean warming?. Front. Mar. Sci., 8.","DOI":"10.3389\/fmars.2021.760637"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"2764","DOI":"10.1038\/s41598-024-53198-7","article-title":"Synergistic effect of nitrate exposure and heatwaves on the growth, and metabolic activity of microalgae, Chlamydomonas reinhardtii, and Pseudo-kirchneriella subcapitata","volume":"14","author":"Akter","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"e231118","DOI":"10.2903\/j.efsa.2025.e231118","article-title":"Algal-derived functional additives for ensured safety and efficacy","volume":"23","author":"Silva","year":"2025","journal-title":"EFSA J."},{"key":"ref_113","doi-asserted-by":"crossref","unstructured":"L\u00f8vdal, T., Lunestad, B.T., Myrmel, M., Rosnes, J.T., and Skipnes, D. (2021). Microbiological food safety of seaweeds. Foods, 10.","DOI":"10.3390\/foods10112719"},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"103579","DOI":"10.1016\/j.algal.2024.103579","article-title":"Algal food safety: Possible contaminations, challenges of harmonized quality assessments, and suggested recommendations for the nascent industry of microalgae-based products","volume":"81","author":"Nematollahi","year":"2024","journal-title":"Algal Res."},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Withana, W., Gunarathna, D., Dissanayake, D., Rathnayake, A., de Zoysa, H., and Waisundara, V.Y. (2025). Microalgae toxins in food products and impact on human health: A review. Front. Nutr., 12.","DOI":"10.3389\/fnut.2025.1603843"},{"key":"ref_116","first-page":"456","article-title":"Reinstatement of the genera Gongolaria Boehmer and Ericaria Stackhouse (Sargassaceae, Phaeophyceae)","volume":"54","author":"Novoa","year":"2020","journal-title":"Eur. J. Phycol."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1080\/09670262.2019.1590862","article-title":"Diversity of Cystoseira sensu lato (Fucales, Phaeophyceae) in the eastern Atlantic and Mediterranean based on morphological and DNA evidence, including Carpodesmia gen. emend. and Treptacantha gen. emend","volume":"54","author":"Orellana","year":"2019","journal-title":"Eur. J. Phycol."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/BF00046555","article-title":"Review of the genera of algae described by Stackhouse","volume":"2","author":"Papenfuss","year":"1950","journal-title":"Hydrobiologia"},{"key":"ref_119","unstructured":"Rubi\u00f1o, S., Aymerich, T., Peteiro, C., Bover-Cid, S., and Hort\u00f3s, M. (2022). Brown Macroalgae (Phaeophyceae) Extracts with Antimicrobial Activity as a Natural Strategy to Improve Food Safety, Centro Oceanogr\u00e1fico de Santander."},{"key":"ref_120","unstructured":"(2018). Methods for Dilution Antimicrobial Susceptibility Test for Bacteria That Grow Aerobically, 11th ed (Standard No. CLSI Standard M07). Approved Standard."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"717","DOI":"10.2116\/analsci.30.717","article-title":"Applicability of the DPPH assay for evaluating the antioxidant capacity of food additives-inter-laboratory evaluation study","volume":"30","author":"Shimamura","year":"2014","journal-title":"Anal. Sci."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/168167a0","article-title":"A colorimetric method for the determination of sugars","volume":"168","author":"Dubois","year":"1951","journal-title":"Nature"},{"key":"ref_123","doi-asserted-by":"crossref","unstructured":"Rubi\u00f1o, S., Peteiro, C., Aymerich, T., and Hort\u00f3s, M. (2022). Brown macroalgae (Phaeophyceae): A valuable reservoir of antimicrobial compounds on northern coast of Spain. Mar. Drugs, 20.","DOI":"10.3390\/md20120775"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1007\/s10811-006-9048-4","article-title":"A simple 96-well microplate method for estimation of total polyphenol content in seaweeds","volume":"18","author":"Zhang","year":"2006","journal-title":"J. Appl. Phycol."}],"container-title":["Marine Drugs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-3397\/24\/8\/257\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:41:02Z","timestamp":1785411662000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-3397\/24\/8\/257"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,25]]},"references-count":125,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["md24080257"],"URL":"https:\/\/doi.org\/10.3390\/md24080257","relation":{},"ISSN":["1660-3397"],"issn-type":[{"value":"1660-3397","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,25]]}}}