{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T04:59:24Z","timestamp":1785560364475,"version":"3.56.0"},"reference-count":21,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 Research and Innovation Programme","award":["101034309"],"award-info":[{"award-number":["101034309"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation Programme","award":["2025\u20132027"],"award-info":[{"award-number":["2025\u20132027"]}]},{"name":"Eco-Safe Ridge Mining project","award":["326881"],"award-info":[{"award-number":["326881"]}]},{"name":"Trond Mohn Foundation (TMF) Centre for Deep-Sea Research"},{"id":[{"id":"https:\/\/ror.org\/018zj6955","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005416","name":"The Research Council of Norway","doi-asserted-by":"crossref","award":["226244\/F50"],"award-info":[{"award-number":["226244\/F50"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"crossref"}]},{"award":["226244\/F50"],"award-info":[{"award-number":["226244\/F50"]}],"id":[{"id":"https:\/\/ror.org\/00epmv149","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mar. Drugs","Marine Drugs"],"abstract":"<jats:p>Marine organisms from deep-sea environments have attracted considerable attention in drug discovery because they produce structurally diverse natural products. However, Norwegian deep-sea ecosystems remain largely unexplored in terms of natural product chemistry and bioactivity. In this study, a deep-sea sponge belonging to the genus Phakellia was selectively collected using a low-impact, remotely operated vehicle approach from the Mohn\u2019s Treasure area in the Norwegian Sea at a depth of 2858 m, representing one of the deepest sponge samples investigated in Norwegian waters to date. Chemical investigation of this specimen resulted in the isolation of three new glycosylated fatty acid amides, phakelliosides A\u2013C (1\u20133), together with two known compounds, 11-(S)-myxillin B (4) and 11-(S)-myxillin C (5), which were isolated as pure individual compounds from natural sources for the first time, with their absolute configurations unambiguously established. These compounds were isolated using a combination of chromatographic techniques, and the structures of the isolated compounds were elucidated by comprehensive spectroscopic analyses, including 1D and 2D NMR and UHPLC\u2013HRMS data. The absolute configurations were determined by optical rotation analysis following acid hydrolysis. Compounds 1\u20135 were subjected to preliminary antibacterial screening against Enterococcus faecalis, Staphylococcus aureus, Streptococcus agalactiae, Escherichia coli, and Pseudomonas aeruginosa at 100 \u00b5g\/mL. Under the screening conditions, compound 3 produced the lowest OD600 value against E. faecalis (OD600 = 0.154), whereas generally higher OD600 values were observed against the Gram-negative strains. To the best of our knowledge, this is the first report describing the preliminary antibacterial screening of glycosylated fatty acid amides isolated from Norwegian deep-sea organisms. These findings expand current knowledge of the chemical diversity of natural products associated with Norwegian deep-sea sponges. The biosynthetic origin of these metabolites remains unresolved and warrants further investigation.<\/jats:p>","DOI":"10.3390\/md24080264","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:55:11Z","timestamp":1785419711000},"page":"264","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and Characterization of Glycosylated Fatty Acid Amides from the Norwegian Deep-Sea Sponge Phakellia sp."],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0564-2606","authenticated-orcid":false,"given":"Le Ba","family":"Vinh","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Technology, University of Bergen (UiB), 5007 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sindre Wesley","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Technology, University of Bergen (UiB), 5007 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Rodr\u00edguez-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Technology, University of Bergen (UiB), 5007 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro A.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"The Centre for Deep-Sea Research, Department of Biological Sciences (BIO), University of Bergen (UiB), 5006 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6804-921X","authenticated-orcid":false,"given":"Monica","family":"Jordheim","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Technology, University of Bergen (UiB), 5007 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s12032-025-03161-x","article-title":"Macrophage-cancer cell crosstalk in breast cancer chemotherapy resistance","volume":"43","author":"Guo","year":"2026","journal-title":"Med. 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