{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T02:39:39Z","timestamp":1792463979727,"version":"4.5.0"},"reference-count":20,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2011,4,18]],"date-time":"2011-04-18T00:00:00Z","timestamp":1303084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Marine Drugs"],"abstract":"<jats:p>Polyhydroxybutyrate (PHB) is one of the polyhydroxyalkanoates (PHAs) which has biodegradable and biocompatible properties. They are adopted in the biomedical field, in, for example, medical implants and drug delivery carriers. This study seeks to promote the production of PHB by Vibrio sp. BM-1, isolated from a marine environment by improving constituents of medium and implementing an appropriate fermentation strategy. This study successfully developed a glycerol-yeast extract-tryptone (GYT) medium that can facilitate the growth of Vibrio sp. BM-1 and lead to the production of 1.4 g\/L PHB at 20 h cultivation. This study also shows that 1.57 g\/L PHB concentration and 16% PHB content were achieved, respectively, when Vibrio sp. BM-1 was cultivated with MS-GYT medium (mineral salts-supplemented GYT medium) for 12 h. Both cell dry weight (CDW) and residual CDW remained constant at around 8.2 g\/L and 8.0 g\/L after the 12 h of cultivation, until the end of the experiment. However, both 16% of PHB content and 1.57 g\/L of PHB production decreased rapidly to 3% and 0.25 g\/L, respectively from 12 h of cultivation to 40 h of cultivation. The results suggest that the secretion of PHB depolymerase that might be caused by the addition of mineral salts reduced PHB after 12 h of cultivation. However, work will be done to explain the effect of adding mineral salts on the production of PHB by Vibrio sp. BM-1 in the near future.<\/jats:p>","DOI":"10.3390\/md9040615","type":"journal-article","created":{"date-parts":[[2011,4,19]],"date-time":"2011-04-19T11:30:13Z","timestamp":1303212613000},"page":"615-624","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Biodegradable and Biocompatible Biomaterial, Polyhydroxybutyrate, Produced by an Indigenous Vibrio sp. BM-1 Isolated from Marine Environment"],"prefix":"10.3390","volume":"9","author":[{"given":"Yu-Hong","family":"Wei","sequence":"first","affiliation":[{"name":"Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Chuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho-Shing","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Material Science, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Om-Murugan","family":"Janarthanan","sequence":"additional","affiliation":[{"name":"Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2011,4,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.mib.2010.02.006","article-title":"Polyhydroxyalkanoates: Bioplastics with a green agenda","volume":"13","author":"Keshavarz","year":"2010","journal-title":"Curr Opin Microbiol"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0960-8524(02)00212-2","article-title":"Polyhydroxyalkanoates: An overview","volume":"87","author":"Reddy","year":"2003","journal-title":"Bioresour Technol"},{"key":"ref_3","first-page":"77","article-title":"Plastics from bacteria and for bacteria: poly(beta-hydroxyalkanoates) as natural, biocompatible, and biodegradable polyesters","volume":"41","author":"Brandl","year":"1990","journal-title":"Adv Biochem Eng Biotechnol"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.procbio.2004.01.053","article-title":"Recent advances in microbial polyhydroxyalkanoates","volume":"40","author":"Khanna","year":"2005","journal-title":"Proc Biochem"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"20785","DOI":"10.1016\/S0021-9258(17)32061-6","article-title":"Biosynthesis of poly-3-hydroxybutyrate in the luminescent bacterium, Vibrio harveyi, and regulation by the lux autoinducer, N-(3-hydroxybutanoyl)homoserine lactone","volume":"269","author":"Sun","year":"1994","journal-title":"J Biol Chem"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/S0167-7799(97)01099-8","article-title":"Biopolymers from marine prokaryotes","volume":"15","author":"Weiner","year":"1997","journal-title":"Trends Biotechnol"},{"key":"ref_7","first-page":"151","article-title":"Fast and sensitive multiple sequence alignments on a microcomputer","volume":"5","author":"Higgins","year":"1989","journal-title":"Comput Appl Biosci"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2289","DOI":"10.1016\/j.procbio.2006.05.026","article-title":"Enzymatic extruded starch as a carbon source for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Haloferax mediterranei","volume":"41","author":"Chen","year":"2006","journal-title":"Process Biochem"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1163\/156856206778937208","article-title":"Preparation and characterizationof poly(hydroxyalkanoate) from the fermentation of Haloferax mediterranei","volume":"17","author":"Don","year":"2006","journal-title":"J Biomater Sci Polym Ed"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1007\/s10295-006-0098-z","article-title":"Production of polyhydroxyalkanoates from inexpensive extruded rice bran and starch by Haloferax mediterranei","volume":"33","author":"Huang","year":"2006","journal-title":"J Ind Microbiol Biotechnol"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2436","DOI":"10.1016\/j.biortech.2008.11.024","article-title":"Statistical media optimization for growth and PHB production from methanol by a methylotrophic bacterium","volume":"100","author":"Shojaosadati","year":"2009","journal-title":"Bioresour Technol"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"151","DOI":"10.2323\/jgam.51.151","article-title":"Degradation of polyesters by a novel marine Nocardiopsis aegyptia sp. nov.: Application of Plackett-Burman experimental design for the improvement of PHB depolymerase activity","volume":"51","author":"Ghanem","year":"2005","journal-title":"J Gen Appl Microbiol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1016\/j.ijbiomac.2010.08.008","article-title":"Strategies of poly(3-hydroxybutyrate) synthesis by Haloarcula sp. 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