{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T12:52:28Z","timestamp":1777639948087,"version":"3.51.4"},"reference-count":51,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2018,4,12]],"date-time":"2018-04-12T00:00:00Z","timestamp":1523491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objective: In Wilson\u2019s disease, copper metabolism is impaired due to defective copper transporting protein ATP7B, resulting in copper accumulation in liver and brain and causing damage to liver and brain tissues. Published data suggest that one of the possible treatments for Wilson\u2019s disease is curcumin\u2014a compound found in the root of Curcuma longa. In this study, we tested whether curcumin affects copper transport and excretion in HepG2 hepatocytes carrying wildtype ATP7B. Materials and Methods: We examined the impact of 5 \u00b5M and 25 \u00b5M curcumin on the transport of copper in HepG2 cells incubated with 20 \u00b5M and 100 \u00b5M CuCl2, as well as copper excretion from cells. First, immunofluorescent staining and co-localization analysis were carried out in HepG2 cells using confocal laser scanning microscope and Nikon NIS Elements software. Second, a concentration of copper extracted into cell culture medium was determined using atomic absorption spectrometry. Results: The analysis of the co-localization between Golgi complex and ATP7B revealed that both 5 \u00b5M and 25 \u00b5M doses of curcumin improve the ability of liver cells to transport copper to plasma membrane at 20 \u00b5M CuCl2, but not at 100 \u00b5M CuCl2 concentration. However, atomic absorption spectrometry showed that curcumin rather promotes copper absorption into liver cell line HepG2 than excretion of it. Conclusions: Curcumin accelerates the transport of copper within liver cells, but does not promote copper excretion from HepG2 cells.<\/jats:p>","DOI":"10.3390\/medicina54020014","type":"journal-article","created":{"date-parts":[[2018,5,8]],"date-time":"2018-05-08T03:17:02Z","timestamp":1525749422000},"page":"14","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Curcumin Effect on Copper Transport in HepG2 Cells"],"prefix":"10.3390","volume":"54","author":[{"given":"Anita","family":"Berzina","sequence":"first","affiliation":[{"name":"August Kirchenstein Institute of Microbiology and Virology, Riga Stradins University, Dzirciema 16, Riga LV-1007, Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inese","family":"Martinsone","sequence":"additional","affiliation":[{"name":"Institute of Occupational Safety and Environmental Health, Riga Stradins University, Dzirciema 16, Riga LV-1007, Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simons","family":"Svirskis","sequence":"additional","affiliation":[{"name":"August Kirchenstein Institute of Microbiology and Virology, Riga Stradins University, Dzirciema 16, Riga LV-1007, Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0971-1784","authenticated-orcid":false,"given":"Modra","family":"Murovska","sequence":"additional","affiliation":[{"name":"August Kirchenstein Institute of Microbiology and Virology, Riga Stradins University, Dzirciema 16, Riga LV-1007, Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martins","family":"Kalis","sequence":"additional","affiliation":[{"name":"August Kirchenstein Institute of Microbiology and Virology, Riga Stradins University, Dzirciema 16, Riga LV-1007, Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,4,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1016\/j.fct.2011.04.004","article-title":"Dual effects of curcumin on neuronal oxidative stress in the presence of Cu(II)","volume":"49","author":"Huang","year":"2011","journal-title":"Food Chem. 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