{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T22:28:21Z","timestamp":1765232901686,"version":"build-2065373602"},"reference-count":21,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,16]],"date-time":"2020-05-16T00:00:00Z","timestamp":1589587200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"YuYu Pharma","award":["YuYu Pharma"],"award-info":[{"award-number":["YuYu Pharma"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and objectives: Alterations in mitochondrial DNA (mtDNA) have been observed and studied in various diseases. However, the clinical value of the mtDNA copy number (mtDNA-CN) alterations in osteonecrosis of the femoral head (ONFH) is poorly understood. In the present study, we investigated whether alterations in mtDNA-CNs are associated with clinicopathological parameters in ONFH. Materials and methods: MtDNA-CNs in the synovial tissue of 34 patients with ONFH and 123 control tissues (femoral neck fracture) were measured using quantitative real-time PCR. The present study then analyzed the correlation between the mtDNA-CN and the clinicopathological characteristics of ONFH and fracture patients. Results: The average mtDNA-CN (mean \u00b1 standard deviation) was 23.82 \u00b1 22.37 and 25.04 \u00b1 24.27 in ONFH and control tissues, respectively, and was not significantly different between the groups (p = 0.792). The mtDNA-CN was positively associated with age (27.7% vs. 45.9%, p = 0.018) and negatively associated with the erythrocyte sedimentation rate (ESR) (11.8% vs. 39.7%, p = 0.024) in all of the samples. The study also found further associations with age (22.2% vs. 68.8%, p = 0.014), gender (30.0% vs. 64.3%, p = 0.048), and ESR (0% vs. 57.7%, p = 0.043) in ONFH. Conclusions: in this study, we demonstrated that mtDNA-CN might be a significant marker for predicting clinical characteristics in ONFH.<\/jats:p>","DOI":"10.3390\/medicina56050239","type":"journal-article","created":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T02:43:42Z","timestamp":1589769822000},"page":"239","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Clinical Characteristics of Mitochondrial DNA Copy Number in Osteonecrosis of the Femoral Head"],"prefix":"10.3390","volume":"56","author":[{"given":"Si-Wook","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, Keimyung University School of Medicine, Dongsan Hospital, Daegu 42601, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4811-574X","authenticated-orcid":false,"given":"Kyung-Jae","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Keimyung University School of Medicine, Dongsan Hospital, Daegu 42601, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8728-512X","authenticated-orcid":false,"given":"Beom-Soo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Keimyung University School of Medicine, Dongsan Hospital, Daegu 42601, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyuk-Jun","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Keimyung University School of Medicine, Dongsan Hospital, Daegu 42601, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5562-0720","authenticated-orcid":false,"given":"Jae-Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine, Keimyung University, Daegu 42601, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,16]]},"reference":[{"key":"ref_1","first-page":"501","article-title":"How rapidly does the human mitochondrial genome evolve?","volume":"59","author":"Howell","year":"1996","journal-title":"Am. 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