{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T02:45:06Z","timestamp":1783651506858,"version":"3.55.0"},"reference-count":29,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T00:00:00Z","timestamp":1635465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: It is of great importance to obtain information about the severity of ischemic damage and duration of testicular torsion for an effective treatment strategy. Nucleolar-organizing regions (NORs) are sites of the ribosomal genes composed of ribosomal DNA and proteins. Post-silver staining NORs are termed \u201cAgNOR\u201d. Since AgNORs clearly reveals the self-renewal potential of cells damaged in ischemic events, we performed the current study. Materials and Methods: The study was carried out in four groups as control, sham, early, and late T\/D. In the surgical groups, testes were corrected after a 4-h ischemia period. Testicular tissue samples were taken on the third day after detorsion in group 1, 2, 3, and on the tenth day after detorsion in group 4. TUNEL and silver stainings were applied to all samples. Results: The differences were significant among the groups for both mean AgNOR number and total AgNOR area\/total nuclear area (TAA\/TNA). Moreover, the differences between control and early torsion-detorsion (T\/D), between control and late T\/D, between sham and early T\/D, between sham and late T\/D, and between early T\/D and late were statistically significant for AgNOR amount. Furthermore, statistically significant differences among the groups for an average number of apoptotic cells per tubule and the percentage of apoptotic tubule values were detected. Discussion: The apoptotic index gives the ratio of cells that are damaged and will die in a programmed way and cells that remain intact, rather than show the viability of the returning testicle. However, by measuring cells that regenerate with AgNOR, we can show not only those that survive but also cells that can repair themselves. Conclusion: AgNOR proteins are usable for the early observation of ischemic injury levels. The amount of AgNOR protein can enlighten us about the extent of testicular damage after T\/D treatment. It may also help the physician in the development of effective treatment strategies for cases.<\/jats:p>","DOI":"10.3390\/medicina57111177","type":"journal-article","created":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T22:22:24Z","timestamp":1635805344000},"page":"1177","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["May Argyrophilic Nucleolar Organizer Regions Be Used as a Biomarker for the Detection of the Degree of Ischemic Damage Instead of Tunel in Testicular Torsion?"],"prefix":"10.3390","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2894-0470","authenticated-orcid":false,"given":"Murat","family":"Kabakl\u0131o\u011flu","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery, Medical Faculty, Duzce University, Duzce 81620, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Recep","family":"Eroz","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Medical Faculty, Aksaray University, Aksaray 68000, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murat","family":"Kaya","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, Medical Faculty, Duzce University, Duzce 81620, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2009","DOI":"10.1016\/j.juro.2011.07.024","article-title":"Pediatric testicular torsion epidemiology using a national database: Incidence, risk of orchiectomy and possible measures toward improving the quality of care","volume":"186","author":"Zhao","year":"2011","journal-title":"J. Urol."},{"key":"ref_2","first-page":"835","article-title":"Testicular torsion: Diagnosis, evaluation, and management","volume":"88","author":"Sharp","year":"2013","journal-title":"Am. Fam. Physician"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2459","DOI":"10.1016\/j.juro.2011.01.016","article-title":"Pediatric testicular torsion: Demographics of national orchiopexy versus orchiectomy rates","volume":"185","author":"Cost","year":"2011","journal-title":"J. Urol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1046\/j.1464-410X.2003.04307.x","article-title":"Testicular function after torsion of the spermatic cord","volume":"92","author":"Visser","year":"2003","journal-title":"BJU Int."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1111\/j.1464-410X.2009.08798.x","article-title":"Protective effect of edaravone, a free-radical scavenger, on ischaemia-reperfusion injury in the rat testis","volume":"105","author":"Tamamura","year":"2010","journal-title":"BJU Int."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.urology.2018.03.009","article-title":"Prospective Evaluation of Predictors of Testis Atrophy After Surgery for Testis Torsion in Children","volume":"116","author":"Grimsby","year":"2018","journal-title":"Urology"},{"key":"ref_7","first-page":"885","article-title":"RNA synthesis and splicing interferes with DNA in situ end labeling techniques used to detect apoptosis","volume":"152","author":"Kockx","year":"1998","journal-title":"Am. J. Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"494","DOI":"10.3109\/10520295.2012.698307","article-title":"Is there any relationship between decreased AgNOR protein synthesis and human hair loss?","volume":"87","author":"Eroz","year":"2012","journal-title":"Biotech. Histochem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"267","DOI":"10.3109\/10520295.2013.769632","article-title":"Argyrophilic nucleolar organizing region associated protein synthesis in hair root cells of humans at different developmental stages and sex","volume":"88","author":"Eroz","year":"2013","journal-title":"Biotech. Histochem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/s40520-014-0263-6","article-title":"Investigation of the age-dependent AgNOR protein level in buccal epithelial cells of healthy individuals","volume":"27","author":"Selvi","year":"2015","journal-title":"Aging Clin. Exp. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1177\/0960327114531994","article-title":"Is one-time carbon monoxide intoxication harmless? Evaluation by argyrophilic nucleolar-organizing regions staining method","volume":"34","author":"Saritas","year":"2015","journal-title":"Hum. Exp. Toxicol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1177\/0960327115579432","article-title":"Can argyrophilic nucleolar organizing region-associated protein amount be used for the detection of cardiac damage?","volume":"35","author":"Afacan","year":"2016","journal-title":"Hum. Exp. Toxicol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1177\/0960327115608929","article-title":"Are there any effects of chronic carbon monoxide exposure on argyrophilic nucleolar-organizing region-associated protein synthesis in rat myocardium?","volume":"35","author":"Saritas","year":"2016","journal-title":"Hum. Exp. Toxicol."},{"key":"ref_14","first-page":"9","article-title":"Evaluation of argyrophilic nucleolar organizing region\u2013associated protein synthesis in femoral muscle cells of rats exposed 3000 ppm carbon monoxide gas","volume":"8","author":"Eroz","year":"2016","journal-title":"Konuralp Med. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1080\/15569543.2018.1525401","article-title":"Use of argyrophilic nucleolar\u2013organizer region-associated protein synthesis in skeletal muscle cells for prediction of chronic carbon monoxide exposure","volume":"39","author":"Gunes","year":"2020","journal-title":"Toxin Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1007\/s12022-011-9161-z","article-title":"The evaluation of argyrophilic nucleolar organizing region proteins in fine needle aspiration samples of thyroid","volume":"22","author":"Eroz","year":"2011","journal-title":"Endocr. Pathol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1002\/cbin.10038","article-title":"Detection and comparison of cutoff values for total AgNOR area\/nuclear area and AgNOR number\/nucleus in benign thyroid nodules and normal thyroid tissue","volume":"37","author":"Eroz","year":"2013","journal-title":"Cell Biol. Int."},{"key":"ref_18","first-page":"226","article-title":"Value of argyrophilic nucleolar organizing region protein determinations in nondiagnostic fine needle aspiration samples (due to insufficient cell groups) of thyroid nodules","volume":"35","author":"Eroz","year":"2013","journal-title":"Anal. Quan. Cytol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"179","DOI":"10.3109\/10520295.2014.976271","article-title":"Argyrophilic nucleolar organizing region associated protein synthesis for cytologic discrimination of follicular thyroid lesions","volume":"90","author":"Oktay","year":"2015","journal-title":"Biotech. Histochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4995","DOI":"10.1177\/0300060518788751","article-title":"The role of argyrophilic nucleolar organizing region-associated proteins in clinical exacerbation of chronic obstructive pulmonary disease","volume":"46","author":"Sonmez","year":"2018","journal-title":"J. Int. Med. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.yexmp.2010.07.006","article-title":"Protective effects of melatonin on testicular torsion\/detorsion-induced ischemia-reperfusion injury in rats","volume":"89","author":"Kanter","year":"2010","journal-title":"Exp. Mol. Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1177\/44.9.8773561","article-title":"In situ apoptotic cell labeling by the TUNEL method: Improvement and evaluation on cell preparations","volume":"44","author":"Negoescu","year":"1996","journal-title":"J. Histochem. Cytochem."},{"key":"ref_23","first-page":"91","article-title":"Chromosome staining and banding techniques","volume":"Volume 1","author":"Rooney","year":"1986","journal-title":"Human Cytogenetics, Constitutional Analysis, Practical Approach"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1177\/41.3.8429207","article-title":"Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR)","volume":"41","author":"Lindner","year":"1993","journal-title":"J. Histochem. Cytochem."},{"key":"ref_25","unstructured":"Rasband, W.S. (2021, September 28). ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, Available online: https:\/\/imagej.nih.gov\/ij\/."},{"key":"ref_26","first-page":"100007C06RE2018","article-title":"Investigation of the effect of capsaicin on AgNOR protein synthesis using staining intensity degree in human colon adenocarcinoma","volume":"4","author":"Eroz","year":"2018","journal-title":"Edorium J. Cell Biol."},{"key":"ref_27","first-page":"653","article-title":"May argyrophilic nucleolar organizing region-associated protein synthesis be used for selecting the most reliable dose of drugs such as rhamnetin in cancer treatments?","volume":"117","author":"Ertekin","year":"2016","journal-title":"Bratisl. Lek. Listy."},{"key":"ref_28","first-page":"21","article-title":"Argyrophilic nucleolar organizing regions associated proteins in oncocytology","volume":"5","author":"Eroz","year":"2015","journal-title":"Oncocytology"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Nakayama, A., Ide, H., Osaka, A., Inoue, Y., Shimomura, Y., Iwahata, T., Kobori, Y., Saito, K., Soh, S., and Okada, H. (2020). The Diagnostic Accuracy of Testicular Torsion by Doctors on Duty Using Sonographic Evaluation with Color Doppler. Am. J. 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