{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T04:42:46Z","timestamp":1775968966246,"version":"3.50.1"},"reference-count":46,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,4,16]],"date-time":"2022-04-16T00:00:00Z","timestamp":1650067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["17704"],"award-info":[{"award-number":["17704"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: The commissural nucleus of the tractus solitarius (cNTS) not only responds to glucose levels directly, but also receives afferent signals from the liver, and from the carotid chemoreceptors (CChR). In addition, leptin, through its receptors in the cNTS, regulates food intake, body weight, blood glucose levels, and brain glucose retention (BGR). These leptin effects on cNTS are thought to be mediated through the sympathetic\u2013adrenal system. How these different sources of information converging in the NTS regulate blood glucose levels and brain glucose retention remains largely unknown. The goal of the present study was to determine whether the local administration of leptin in cNTS alone, or after local anoxic stimulation using sodium cyanide (NaCN) in the carotid sinus, modifies the expression of leptin Ob-Rb and of c-Fos mRNA. We also investigated how leptin, alone, or in combination with carotid sinus stimulation, affected brain glucose retention. Materials and Methods: The experiments were carried out in anesthetized male Wistar rats artificially ventilated to maintain homeostatic values for pO2, pCO2, and pH. We had four groups: (a) experimental 1, leptin infusion in cNTS and NaCN in the isolated carotid sinus (ICS; n = 10); (b) experimental 2, leptin infusion in cNTS and saline in the ICS (n = 10); (c) control 1, artificial cerebrospinal fluid (aCSF) in cNTS and NaCN in the ICS (n = 10); (d) control 2, aCSF in cNTS and saline in the ICS (n = 10). Results: Leptin in cNTS, preceded by NaCN in the ICS increased BGR and leptin Ob-Rb mRNA receptor expression, with no significant increases in c-Fos mRNA in the NTSc. Conclusions: Leptin in the cNTS enhances brain glucose retention induced by an anoxic stimulus in the carotid chemoreceptors, through an increase in Ob-Rb receptors, without persistent changes in neuronal activation.<\/jats:p>","DOI":"10.3390\/medicina58040550","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T01:03:01Z","timestamp":1650330181000},"page":"550","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Leptin in the Commissural Nucleus of the Tractus Solitarius (cNTS) and Anoxic Stimulus in the Carotid Body Chemoreceptors Increases cNTS Leptin Signaling Receptor and Brain Glucose Retention in Rats"],"prefix":"10.3390","volume":"58","author":[{"given":"M\u00f3nica","family":"Lemus","sequence":"first","affiliation":[{"name":"Neuroendocrinology Department, University Center Biomedical Research, Colima University, Colima 28045, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia","family":"Mojarro","sequence":"additional","affiliation":[{"name":"Neuroendocrinology Department, University Center Biomedical Research, Colima University, Colima 28045, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Montero","sequence":"additional","affiliation":[{"name":"Neuroendocrinology Department, University Center Biomedical Research, Colima University, Colima 28045, Mexico"},{"name":"Medicine Faculty, Colima University, Colima 28040, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Ram\u00edrez-Flores","sequence":"additional","affiliation":[{"name":"Neuroendocrinology Department, University Center Biomedical Research, Colima University, Colima 28045, Mexico"},{"name":"Medicine Faculty, Colima University, Colima 28040, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Torres-Magallanes","sequence":"additional","affiliation":[{"name":"Neuroendocrinology Department, University Center Biomedical Research, Colima University, Colima 28045, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adri\u00e1n","family":"Maturano-Melgoza","sequence":"additional","affiliation":[{"name":"Nursing Faculty, Colima University, Colima 28040, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Roces de \u00c1lvarez-Buylla","sequence":"additional","affiliation":[{"name":"Neuroendocrinology Department, University Center Biomedical Research, Colima University, Colima 28045, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2613","DOI":"10.1210\/en.2004-0032","article-title":"Minireview: A hypothalamic role in energy balance with special emphasis on leptin","volume":"145","author":"Sahu","year":"2004","journal-title":"Endocrinology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1111\/j.2040-1124.2012.00203.x","article-title":"The role of leptin in glucose homeostasis","volume":"3","author":"Denroche","year":"2012","journal-title":"J. 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