{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:19:15Z","timestamp":1792095555827,"version":"4.4.0"},"reference-count":38,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:00:00Z","timestamp":1653436800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and objectives: Breast cancer is the most commonly diagnosed cancer in women and its mortality is increasing. Therefore, research to improve treatment is of paramount importance. One method of treatment is photodynamic therapy. Photodynamic therapy selectively stimulates apoptosis in photosensitizer-treated neoplastic breast cells as a result of cytotoxic singlet oxygen generation via collisions between triplet excited state photosensitizer and triplet ground state oxygen upon tissue irradiation. The aim of this study was to evaluate the effects of photodynamic action on cancerous breast tissue samples as a model of photodynamic therapy. Materials and Methods: Breast cancer tissue samples were obtained from post-operative material and the patterns of histopathological changes in breast cancer tissue before and after photodynamic action on post-chemotherapy tissue were evaluated. Excised tissue samples were obtained from 48 female breast cancer patients who had previously undergone chemotherapy. Breast cancer tissues for this study were taken from macroscopically visible tumors larger than 10 mm. Histopathological analysis was performed to evaluate any morphological changes prior to and after photodynamic action on the post-chemotherapy tissue samples. Eighteen breast cancer tissue samples were analyzed before chemotherapy, fifteen after chemotherapy, and fifteen samples were analyzed after chemotherapy and application of photodynamic action. The photosensitizer Rose Bengal was applied to the samples subjected to photodynamic action. Results: Photodynamic action on post-chemotherapy neoplastic tissue showed histological changes under a light microscope. The results showed that morphological changes in breast cancer tissues after chemotherapy and photodynamic action were dependent on the concentration of Rose Bengal. In all cases, follow-up imaging showed tumor shrinkage of an average of 35% from baseline size. Conclusions: Histopathological examination revealed photosensitizer-concentration-dependent changes after photodynamic action in excised post-chemotherapy tissue. The effects of photodynamic action observed in this study suggest that the application of photodynamic therapy after chemotherapy can aid in breast cancer cell eradication.<\/jats:p>","DOI":"10.3390\/medicina58060700","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T05:12:27Z","timestamp":1653455547000},"page":"700","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Histopathological Analysis of the Effect of Photodynamic Action on Post-Chemotherapy Excised Breast Cancer Tissue"],"prefix":"10.3390","volume":"58","author":[{"given":"El\u017cbieta","family":"Osta\u0144ska","sequence":"first","affiliation":[{"name":"Clinical Department of Pathology, Frederick Chopin Clinical Provincial Hospital No. 1, 35-055 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7567-3782","authenticated-orcid":false,"given":"Edyta","family":"Barna\u015b","sequence":"additional","affiliation":[{"name":"Department of Midwifery, Medical College of the University of Rzesz\u00f3w, University of Rzesz\u00f3w, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5557-5464","authenticated-orcid":false,"given":"Dorota","family":"Bartusik-Aebisher","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and General Chemistry, Medical College of the University of Rzesz\u00f3w, University of Rzesz\u00f3w, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6851-5869","authenticated-orcid":false,"given":"Klaudia","family":"Dynarowicz","sequence":"additional","affiliation":[{"name":"Center for Innovative Research in Medical and Natural Sciences, Medical College of the University of Rzesz\u00f3w, University of Rzesz\u00f3w, 35-310 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5947-9867","authenticated-orcid":false,"given":"Magdalena","family":"Szpunar","sequence":"additional","affiliation":[{"name":"Students English Division Science Club, Medical College of the University of Rzesz\u00f3w, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1679-264X","authenticated-orcid":false,"given":"Joanna","family":"Skr\u0119t-Magier\u0142o","sequence":"additional","affiliation":[{"name":"Department of Midwifery, Medical College of the University of Rzesz\u00f3w, University of Rzesz\u00f3w, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2661-6570","authenticated-orcid":false,"given":"David","family":"Aebisher","sequence":"additional","affiliation":[{"name":"Department of Photomedicine and Physical Chemistry, Medical College of the University of Rzesz\u00f3w, University of Rzesz\u00f3w, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"7","author":"Sung","year":"2021","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","first-page":"1339","article-title":"Treatment of breast cancer","volume":"81","author":"Maughan","year":"2010","journal-title":"Am. Fam. Physician"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.2147\/DDDT.S253961","article-title":"Evaluation of the Efficacy of Neoadjuvant Chemotherapy for Breast Cancer","volume":"14","author":"Wang","year":"2020","journal-title":"Drug Des. Devel. Ther."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.soc.2017.01.013","article-title":"Updates in the Treatment of Breast Cancer with Radiotherapy","volume":"26","author":"Castaneda","year":"2017","journal-title":"Surg. Oncol. Clin. N. Am."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"413","DOI":"10.21614\/chirurgia.112.4.413","article-title":"Hormone Therapy in Breast Cancer","volume":"112","author":"Carmocan","year":"2017","journal-title":"Chirurgia"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e841","DOI":"10.1016\/j.clbc.2018.07.023","article-title":"Genetic Markers in Triple-Negative Breast Cancer","volume":"18","author":"Sporikova","year":"2018","journal-title":"Clin. Breast Cancer"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.phrs.2018.11.014","article-title":"Small molecule inhibitors targeting the EGFR\/ErbB family of protein-tyrosine kinases in human cancers","volume":"139","author":"Roskoski","year":"2019","journal-title":"Pharmacol. Res."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Dias, C.J., Helguero, L., and Faustino, M.A.F. (2021). Current Photoactive Molecules for Targeted Therapy of Triple-Negative Breast Cancer. Molecules, 26.","DOI":"10.3390\/molecules26247654"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2120","DOI":"10.18632\/oncotarget.27596","article-title":"Review: Organic nanoparticle based active targeting for photodynamic therapy treatment of breast cancer cells","volume":"11","author":"Montaseri","year":"2020","journal-title":"Oncotarget"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Mokoena, D.R., George, B.P., and Abrahamse, H. (2019). Enhancing Breast Cancer Treatment Using a Combination of Cannabidiol and Gold Nanoparticles for Photodynamic Therapy. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20194771"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Shi, X., Yang, X., Liu, M., Wang, R., Qiu, N., Liu, Y., Yang, H., Ji, J., and Zha, G. (2021). Chondroitin sulfate-based nanoparticles for enhanced chemo-photodynamic therapy overcoming multidrug resistance and lung metastasis of breast cancer. Carbohydr. Polym., 254.","DOI":"10.1016\/j.carbpol.2020.117459"},{"key":"ref_12","unstructured":"Narayan, R. (2017). Monitoring and Evaluation of Biomaterials and Their Performance In Vivo, Elsevier Ltd."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Gunaydin, G., Gedik, M.E., and Ayan, S. (2021). Photodynamic Therapy-Current Limitations and Novel Approaches. Front. Chem., 9.","DOI":"10.3389\/fchem.2021.691697"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1038\/nrc1071","article-title":"Photodynamic Therapy for Cancer","volume":"3","author":"Dolmans","year":"2003","journal-title":"Nat. Rev. Cancer"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"317","DOI":"10.7150\/thno.5284","article-title":"Upconversion Nanoparticles for Photodynamic Therapy and Other Cancer Therapeutics","volume":"3","author":"Wang","year":"2013","journal-title":"Theranostics"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1186\/s12951-020-00697-0","article-title":"The microneedles carrying cisplatin and IR820 to perform synergistic chemo-photodynamic therapy against breast cancer","volume":"18","author":"Fu","year":"2020","journal-title":"J. Nanobiotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.1016\/j.apsb.2020.10.009","article-title":"GSH-responsive SN38 dimer-loaded shape-transformable nanoparticles with iRGD for enhancing chemo-photodynamic therapy","volume":"10","author":"Lin","year":"2020","journal-title":"Acta Pharm. Sin. B"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6531","DOI":"10.2147\/IJN.S256477","article-title":"Guiding Appropriate Timing of Laser Irradiation by Polymeric Micelles for Maximizing Chemo-Photodynamic Therapy","volume":"15","author":"Zhu","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Mohapatra, A., Uthaman, S., and Park, I.K. (2021). External and Internal Stimuli-Responsive Metallic Nanotherapeutics for Enhanced Anticancer Therapy. Front. Mol. Biosci., 7.","DOI":"10.3389\/fmolb.2020.597634"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"76004","DOI":"10.1117\/1.JBO.18.7.076004","article-title":"Feasibility study of the optical imaging of a breast cancer lesion labeled with upconversion nanoparticle biocomplexes","volume":"18","author":"Grebenik","year":"2013","journal-title":"J. Biomed. Opt."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1093\/toxsci\/kfz086","article-title":"Preclinical Study of Biofunctional Polymer-Coated Upconversion Nanoparticles","volume":"70","author":"Guryev","year":"2019","journal-title":"Toxicol. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1016\/j.cclet.2021.10.054","article-title":"Diketopyrrolopyrrole-derived organic small molecular dyes for tumor phototheranostics","volume":"33","author":"Ma","year":"2022","journal-title":"Chin. Chem. Lett."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1007\/s40242-021-1190-7","article-title":"Near-infrared Aza-BODIPY Dyes Through Molecular Surgery for Enhanced Photothermal and Photodynamic Antibacterial Therapy","volume":"37","author":"Yu","year":"2021","journal-title":"Chem. Res. Chin. Univ."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Chen, D., Dai, H., Wang, W., Cai, Y., Mou, X., Zou, J., Shao, J., Mao, Z., Zhong, L., and Dong, X. (2022). Proton-Driven Transformable 1O2-Nanotrap for Dark and Hypoxia Tolerant Photod. Adv. Sci., 14.","DOI":"10.1002\/advs.202200128"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"32372","DOI":"10.1021\/acsami.0c07503","article-title":"NIR-Triggered Sequentially Responsive Nanocarriers Amplified Cascade Synergistic Effect of Chemo-Photodynamic Therapy with Inspired Antitumor Immunity","volume":"12","author":"Jin","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.jconrel.2019.12.043","article-title":"A light-triggered self-reinforced nanoagent for targeted chemo-photodynamic therapy of breast cancer bone metastases via ER stress and mitochondria mediated apoptotic pathways","volume":"319","author":"Huang","year":"2020","journal-title":"J. Control Release"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1039\/C5NR07724A","article-title":"Actively targeting D-\u03b1-tocopheryl polyethylene glycol 1000 succinate-poly(lactic acid) nanoparticles as vesicles for chemo-photodynamic combination therapy of doxorubicin-resistant breast cancer","volume":"8","author":"Jiang","year":"2016","journal-title":"Nanoscale"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"7665","DOI":"10.2147\/IJN.S214377","article-title":"Magnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo\/Photodynamic Therapy","volume":"14","author":"Wang","year":"2019","journal-title":"Int. J. Nanomed."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1080\/10717544.2020.1765431","article-title":"Combined photodynamic chemotherapy investigation of cancer cells using carbon quantum dot-based drug carrier system","volume":"27","author":"Li","year":"2020","journal-title":"Drug Deliv."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"20598","DOI":"10.1039\/C9NR06535K","article-title":"Novel anti-HER2 peptide- conjugated theranostic nanoliposomes combining NaYF4Yb,Er nanoparticles for NIR-activated bioimaging and chemo-photodynamic therapy against breast cancer","volume":"11","author":"Panikar","year":"2019","journal-title":"Nanoscale"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.ejmech.2018.03.077","article-title":"A tumor-targeted Ganetespib-zinc phthalocyanine conjugate for synergistic chemo-photodynamic therapy","volume":"151","author":"Huang","year":"2018","journal-title":"Eur. J. Med. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.carbpol.2018.09.035","article-title":"Sequentially self-assembled polysaccharide-based nanocomplexes for combined chemotherapy and photodynamic therapy of breast cancer","volume":"203","author":"Wang","year":"2019","journal-title":"Carbohydr. Polym."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.7150\/thno.22989","article-title":"Oxygen-generating Hybrid Polymeric Nanoparticles with Encapsulated Doxorubicin and Chlorin e6 for Trimodal Imaging-Guided Combined Chemo-Photodynamic Therapy","volume":"8","author":"Hu","year":"2018","journal-title":"Theranostics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"9157","DOI":"10.1039\/C7TB02359F","article-title":"A reactive oxygen species (ROS)-responsive low molecular weight gel co-loaded with doxorubicin and Zn(ii) phthalocyanine tetrasulfonic acid for combined chemophotodynamic therapy","volume":"5","author":"Xu","year":"2017","journal-title":"J. Mater. Chem. B"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Liu, R., Xu, F., Wang, L., Liu, M., Cao, X., Shi, X., and Guo, R. (2021). Polydopamine-coated laponite nanoplatforms for photoacoustic imaging- guided chemo-phototherapy of breast cancer. Nanomaterials, 11.","DOI":"10.3390\/nano11020394"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1021\/acs.biomac.0c00061","article-title":"Drug-loaded photosensitizer- chitosan nanoparticles for combinatorial chemo-and photodynamic- therapy of cancer","volume":"21","author":"Pandya","year":"2020","journal-title":"Biomacromolecules"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Gaio, E., Conte, C., Esposito, D., Reddi, E., Quaglia, F., and Moret, F. (2020). CD44 Targeting Mediated by Polymeric Nanoparticles and Combination of Chlorine TPCS 2a-PDT and Docetaxel-Chemotherapy for Efficient Killing of Breast Differentiated and Stem Cancer Cells In Vitro. Cancers, 12.","DOI":"10.3390\/cancers12020278"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.actbio.2018.10.019","article-title":"PEGylated hydrazided gold nanorods for pH-triggered chemo\/photodynamic\/photothermal triple therapy of breast cancer","volume":"82","author":"Xu","year":"2018","journal-title":"Acta Biomater."}],"container-title":["Medicina"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1648-9144\/58\/6\/700\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:18:04Z","timestamp":1760138284000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1648-9144\/58\/6\/700"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,25]]},"references-count":38,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["medicina58060700"],"URL":"https:\/\/doi.org\/10.3390\/medicina58060700","relation":{},"ISSN":["1648-9144"],"issn-type":[{"value":"1648-9144","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,25]]}}}