{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:44:27Z","timestamp":1760244267835,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,9,2]],"date-time":"2022-09-02T00:00:00Z","timestamp":1662076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and objectives: Bladder stimulation upregulates neurotrophins associated with voiding reflex. Bacterial cystitis can be a stimulant that activates this system, resulting in a pathological state. Phosphorylated responsive element of binding protein (p-CREB) is a pivotal transcriptional factor in the neurotrophin signaling cascade. The goal of our study was to examine the change in expression of p-CREB in dorsal root ganglia (DRG) of rats after uropathogenic Escherichia coli infection of the bladder. Materials and methods: A total of 19 adult female Sprague\u2013Dawley rats were induced with acute E. coli infection (n = 7), chronic E. coli infection (n = 6), or served as controls (n = 6). In each group, the profiles of p-CREB cell were counted in 6\u201310 sections of each of the DRG collected. DRG cells exhibiting intense nuclear staining were considered to be positive for p-CREB immunoreactivity (p-CREB-IR). Results: Overall, the immunoreactivity of p-CREB was examined in smaller cell profiles with nuclear staining or nuclear and cytoplasmic staining in the DRGs (L1\u2013L6, S1). In the chronic cystitis group, p-CREB-IR in the L1\u2013L6 and S1 DRG was significantly higher than the control group (p &lt; 0.05). Further, p-CREB-IR in the L3\u2013L6 and S1 DRG of the chronic cystitis group was significantly greater than that in the acute cystitis group (p &lt; 0.05). In the control and acute cystitis groups, p-CREB-IR in the L4\u2013L5 DRG was significantly lower than that found in the other DRG sections (p &lt; 0.05). Conclusions: Altogether, acute or chronic E.coli cystitis changed the immunoreactivity of p-CREB in lumbosacral DRG cells. In particular, chronic E. coli infection triggered p-CREB overexpression in L1\u2013L6 and S1 DRG, indicating subsequent pathologic changes.<\/jats:p>","DOI":"10.3390\/medicina58091210","type":"journal-article","created":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T22:33:01Z","timestamp":1662503581000},"page":"1210","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cystitis Induces Altered CREB Expression Related with Micturition Reflex"],"prefix":"10.3390","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2800-5773","authenticated-orcid":false,"given":"Taesoo","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Urology, School of Medicine, Kyung Hee University, Seoul 05278, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-Gi","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Urology, School of Medicine, Kyung Hee University, Seoul 05278, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1046\/j.1464-410X.1997.00097.x","article-title":"Increased nerve growth factor levels in the urinary bladder of women with idiopathic sensory urgency and interstitial cystitis","volume":"79","author":"Lowe","year":"1997","journal-title":"Br. 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