{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T12:18:36Z","timestamp":1781180316307,"version":"3.54.1"},"reference-count":23,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T00:00:00Z","timestamp":1665446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: Myocardial perfusion imaging (MPI) has an important role in the non-invasive investigation of coronary artery disease. The interpretation of MPI studies is mainly based on the visual evaluation of the reconstructed images, while automated quantitation methods may add useful data for each patient. However, little evidence is currently available regarding the actual incremental clinical diagnostic performance of automated MPI analysis. In the present study, we aimed to assess the correlation between automated measurements of Summed Stress Score (SSS), Summed Rest Score (SRS) and Summed Difference Score (SDS), with the corresponding expert reading values, using coronary angiography as the gold standard. Materials and Methods: The study was conducted at the Nuclear Medicine Laboratory of the University Hospital of Larissa, Larissa, Greece, \u03bfver an one-year period (January 2019\u2013January 2020). 306 patients, with known or suspected coronary artery disease, were enrolled in the study. Each participant underwent a coronary angiography, prior to or after the scintigraphic study (within a three-month period). Either symptom-limited treadmill test, or pharmacologic testing using adenosine or regadenoson, was performed in all participants, and the scintigraphic studies were carried out using technetium 99m (99mTc) tetrofosmin (one-day stress\/rest protocol). Coronary angiographies were scored according to a 4-point scoring system (angiographic score; O: normal study, 1: one-vessel disease, 2: two-vessel disease, 3: three-vessel disease). Moreover, automated measurements of SSS, SRS and SDS were derived by three widely available software packages (Emory Cardiac Toolbox, Myovation, Quantitative Perfusion SPECT). Results: Interclass Correlation Coefficients of SSS, SRS and SDS between expert reading and software packages were moderate to excellent. Visually defined SSS, SRS and SDS were significantly correlated with the corresponding results of all software packages. However, visually defined SSS, SRS and SDS were more strongly correlated with the angiographic score, indicating a better performance of expert reading when compared to automated analysis. Conclusions: Based on our results, visual evaluation continues to have a crucial role for the interpretation of MPI images. Software packages can provide automated measurements of several parameters, particularly contributing to the investigation of cases with ambiguous scintigraphic findings.<\/jats:p>","DOI":"10.3390\/medicina58101432","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T06:13:27Z","timestamp":1665468807000},"page":"1432","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Automated Analysis vs. Expert Reading in Nuclear Cardiology: Correlations with the Angiographic Score"],"prefix":"10.3390","volume":"58","author":[{"given":"George","family":"Angelidis","sequence":"first","affiliation":[{"name":"Nuclear Medicine Laboratory, University of Thessaly, University Hospital of Larissa, Mezourlo, 41110 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8439-6708","authenticated-orcid":false,"given":"Varvara","family":"Valotassiou","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Laboratory, University of Thessaly, University Hospital of Larissa, Mezourlo, 41110 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Tsougos","sequence":"additional","affiliation":[{"name":"Medical Physics Laboratory, University of Thessaly, University Hospital of Larissa, Mezourlo, 41110 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chara","family":"Tzavara","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Laboratory, University of Thessaly, University Hospital of Larissa, Mezourlo, 41110 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios","family":"Psimadas","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Laboratory, University of Thessaly, University Hospital of Larissa, Mezourlo, 41110 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evdoxia","family":"Theodorou","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Laboratory, University of Thessaly, University Hospital of Larissa, Mezourlo, 41110 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Ziaka","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Laboratory, University of Thessaly, University Hospital of Larissa, Mezourlo, 41110 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stavroula","family":"Giannakou","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Laboratory, University of Thessaly, University Hospital of Larissa, Mezourlo, 41110 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charalampos","family":"Ziangas","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Laboratory, University of Thessaly, University Hospital of Larissa, Mezourlo, 41110 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7159-2478","authenticated-orcid":false,"given":"John","family":"Skoularigis","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University of Thessaly, University Hospital of Larissa, Mezourlo, 41110 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippos","family":"Triposkiadis","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University of Thessaly, University Hospital of Larissa, Mezourlo, 41110 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panagiotis","family":"Georgoulias","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Laboratory, University of Thessaly, University Hospital of Larissa, Mezourlo, 41110 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1161\/CIRCIMAGING.109.854893","article-title":"Noninvasive assessment of myocardial perfusion","volume":"2","author":"Salerno","year":"2009","journal-title":"Circ. Cardiovasc. Imaging"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1067\/mnc.2002.123122","article-title":"Standardized myocardial segmentation and nomenclature for tomographic imaging of the heart. A statement for healthcare professionals from the Cardiac Imaging Committee of the Council on Clinical Cardiology of the American Heart Association","volume":"9","author":"Cerqueira","year":"2002","journal-title":"J. Nucl. Cardiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1007\/s12350-017-1181-8","article-title":"Impact of renin-angiotensin-aldosterone system polymorphisms on myocardial perfusion: Correlations with myocardial single photon emission computed tomography-derived parameters","volume":"26","author":"Angelidis","year":"2019","journal-title":"J. Nucl. Cardiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.nuclcard.2007.06.008","article-title":"Quantitation in gated perfusion SPECT imaging: The Cedars-Sinai approach","volume":"14","author":"Germano","year":"2007","journal-title":"J. Nucl. Cardiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.nuclcard.2007.06.006","article-title":"Corridor4DM: The Michigan method for quantitative nuclear cardiology","volume":"14","author":"Ficaro","year":"2007","journal-title":"J Nucl. Cardiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.nuclcard.2007.06.009","article-title":"The increasing role of quantification in clinical nuclear cardiology: The Emory approach","volume":"14","author":"Garcia","year":"2007","journal-title":"J. Nucl. Cardiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/s12350-013-9735-x","article-title":"High-efficiency SPECT MPI: Comparison of automated quantification, visual interpretation, and coronary angiography","volume":"20","author":"Duvall","year":"2013","journal-title":"J. Nucl. Cardiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"221","DOI":"10.2967\/jnumed.112.108969","article-title":"Comparison of fully automated computer analysis and visual scoring for detection of coronary artery disease from myocardial perfusion SPECT in a large population","volume":"54","author":"Arsanjani","year":"2013","journal-title":"J. Nucl. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1007\/s00259-018-3951-1","article-title":"Automated SPECT analysis compared with expert visual scoring for the detection of FFR-defined coronary artery disease","volume":"45","author":"Driessen","year":"2018","journal-title":"Eur. J. Nucl. Med. Mol. Imaging"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1929","DOI":"10.1007\/s00259-015-3139-x","article-title":"EANM procedural guidelines for radionuclide myocardial perfusion imaging with SPECT and SPECT\/CT: 2015 revision","volume":"42","author":"Verberne","year":"2015","journal-title":"Eur. J. Nucl. Med. Mol. Imaging"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1007\/s00259-005-1779-y","article-title":"EANM\/ESC procedural guidelines for myocardial perfusion imaging in nuclear cardiology","volume":"32","author":"Hesse","year":"2005","journal-title":"Eur. J. Nucl. Med. Mol. Imaging"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1007\/s00259-002-0998-8","article-title":"A comparison of three radionuclide myocardial perfusion tracers in clinical practice: The ROBUST study","volume":"29","author":"Kapur","year":"2002","journal-title":"Eur. J. Nucl. Med. Mol. Imaging"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1097\/MNM.0b013e328013ebd7","article-title":"Evaluation of abnormal heart-rate recovery after exercise testing in patients with diabetes mellitus: Correlation with myocardial SPECT and chronotropic parameters","volume":"28","author":"Georgoulias","year":"2007","journal-title":"Nucl. Med. Commun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1007\/s12350-016-0757-z","article-title":"Quantitative measurements of myocardial perfusion and function from SPECT (and PET) studies depend on the method used to perform those measurements","volume":"25","author":"Germano","year":"2018","journal-title":"J. Nucl. Cardiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.jcm.2016.02.012","article-title":"A Guideline of Selecting and Reporting Intraclass Correlation Coefficients for Reliability Research","volume":"15","author":"Koo","year":"2016","journal-title":"J. Chiropr. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1001\/jama.2014.472","article-title":"Population trends from 2000\u20132011 in nuclear myocardial perfusion imaging use","volume":"311","author":"McNulty","year":"2014","journal-title":"JAMA."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1007\/s12350-010-9220-8","article-title":"Combined quantitative analysis of attenuation corrected and noncorrected myocardial perfusion SPECT: Method development and clinical validation","volume":"17","author":"Xu","year":"2010","journal-title":"J. Nucl. Cardiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.ccl.2015.08.003","article-title":"Automated quantitative nuclear cardiology methods","volume":"34","author":"Motwani","year":"2016","journal-title":"Cardiol. Clin."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.nuclcard.2007.09.020","article-title":"Quantitative myocardial-perfusion SPECT: Comparison of three state-of-the-art software packages","volume":"15","author":"Wolak","year":"2008","journal-title":"J. Nucl. Cardiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1007\/s00259-007-0600-5","article-title":"Comparison of SSS and SRS calculated from normal databases provided by QPS and 4D-MSPECT manufacturers and from identical institutional normals","volume":"35","author":"Knollmann","year":"2008","journal-title":"Eur. J. Nucl. Med. Mol. Imaging"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/2191-219X-1-22","article-title":"Diagnostic evaluation of three cardiac software packages using a consecutive group of patients","volume":"1","author":"Johansson","year":"2011","journal-title":"EJNMMI Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1097\/RLU.0b013e31826a8051","article-title":"Quantitative myocardial perfusion-SPECT: Algorithm-specific influence of reorientation on calculation of summed stress score","volume":"37","author":"Knollmann","year":"2012","journal-title":"Clin. Nucl. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1007\/s12350-016-0730-x","article-title":"Myocardial perfusion and left ventricular quantitative parameters obtained using gated myocardial SPECT: Comparison of three software packages","volume":"25","author":"Alexiou","year":"2018","journal-title":"J. Nucl. 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