{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T08:09:39Z","timestamp":1762070979689,"version":"build-2065373602"},"reference-count":44,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T00:00:00Z","timestamp":1665446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008027","name":"University of California Institute for Mexico and the United States, Mexico\u2019s National Council for Science and Technology (UC MEXUS-CONACYT)","doi-asserted-by":"publisher","award":["CN-19-194","USO513006176"],"award-info":[{"award-number":["CN-19-194","USO513006176"]}],"id":[{"id":"10.13039\/100008027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010387","name":"Universidad de Sonora","doi-asserted-by":"publisher","award":["CN-19-194","USO513006176"],"award-info":[{"award-number":["CN-19-194","USO513006176"]}],"id":[{"id":"10.13039\/501100010387","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: Perilipins 1\u20135 (PLIN) are lipid droplet-associated proteins that participate in regulating lipid storage and metabolism, and the PLIN5 isoform is known to form a nuclear complex with peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1\u03b1) to regulate lipid metabolism gene expression. However, the changes in PLIN isoforms\u2019 expression in response to pregnancy-induced cardiac hypertrophy are not thoroughly studied. The aim of this study was to quantify the mRNA expression of PLIN isoforms and PGC-1\u03b1 along with total triacylglycerol (TAG) and cholesterol levels during late pregnancy and the postpartum period in the rat left ventricle. Materials and Methods: Female Sprague-Dawley rats were divided into three groups: non-pregnant, late pregnancy, and postpartum. The mRNA and protein levels were evaluated using quantitative RT-PCR and Western blotting, respectively. TAG and total cholesterol content were evaluated using commercial colorimetric methods. Results: The expression of mRNAs for PLIN1, 2, and 5 increased during pregnancy and the postpartum period. PGC-1\u03b1 mRNA and protein expression increased during pregnancy and the postpartum period. Moreover, TAG and total cholesterol increased during pregnancy and returned to basal levels after pregnancy. Conclusions: Our results demonstrate that pregnancy upregulates differentially the expression of PLIN isoforms along with PGC-1\u03b1, suggesting that together they might be involved in the regulation of the lipid metabolic shift induced by pregnancy.<\/jats:p>","DOI":"10.3390\/medicina58101433","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T22:18:13Z","timestamp":1665526693000},"page":"1433","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Perilipin Isoforms and PGC-1\u03b1 Are Regulated Differentially in Rat Heart during Pregnancy-Induced Physiological Cardiac Hypertrophy"],"prefix":"10.3390","volume":"58","author":[{"given":"Jes\u00fas A.","family":"Rosas-Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Departamento de Ciencias Qu\u00edmico-Biol\u00f3gicas y Agropecuarias, Universidad de Sonora, L\u00e1zaro C\u00e1rdenas del R\u00edo No. 100, Francisco Villa, Navojoa CP 85880, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adolfo","family":"Virgen-Ort\u00edz","sequence":"additional","affiliation":[{"name":"Centro Universitario de Investigaciones Biom\u00e9dicas, Universidad de Colima, Colima 28040, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8738-8199","authenticated-orcid":false,"given":"Enrico A.","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Departamento de Zoolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Ciudad de M\u00e9xico 07738, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3715-7194","authenticated-orcid":false,"given":"Rudy M.","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Department of Molecular & Cell Biology, University of California Merced, Merced, CA 95343, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 G.","family":"So\u00f1anez-Organis","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Qu\u00edmico-Biol\u00f3gicas y Agropecuarias, Universidad de Sonora, L\u00e1zaro C\u00e1rdenas del R\u00edo No. 100, Francisco Villa, Navojoa CP 85880, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2176","DOI":"10.1016\/j.febslet.2010.03.022","article-title":"Lipid droplets: A dynamic organelle moves into focus","volume":"584","author":"Beller","year":"2010","journal-title":"FEBS Lett."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1002\/emmm.201100671","article-title":"Balancing the fat: Lipid droplets and human disease","volume":"5","author":"Krahmer","year":"2013","journal-title":"EMBO Mol. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"11341","DOI":"10.1016\/S0021-9258(18)99168-4","article-title":"Perilipin, a major hormonally regulated adipocyte-specific phosphoprotein associated with the periphery of lipid storage droplets","volume":"266","author":"Greenberg","year":"1991","journal-title":"J. Biol. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1194\/jlr.R000034","article-title":"Adoption of PERILIPIN as a unifying nomenclature for the mammalian PAT-family of intracellular lipid storage droplet proteins","volume":"51","author":"Kimmel","year":"2010","journal-title":"J. Lipid Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1007\/s00335-01-2055-5","article-title":"The murine perilipin gene: The lipid droplet-associated perilipins derive from tissue-specific, mRNA splice variants and define a gene family of ancient origin","volume":"12","author":"Lu","year":"2001","journal-title":"Mamm. Genome"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"32253","DOI":"10.1074\/jbc.M204410200","article-title":"Functional conservation for lipid storage droplet association among perilipin, ADRP, and TIP47 (PAT)-related proteins in mammals, Drosophila, and Dictyostelium","volume":"277","author":"Miura","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1186\/s12944-017-0473-y","article-title":"Perilipins: A diversity of intracellular lipid droplet proteins","volume":"16","author":"Itabe","year":"2017","journal-title":"Lipids Health Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1161\/01.ATV.0000223345.11820.da","article-title":"Adipocyte differentiation-related protein promotes fatty acid storage in cytosolic triglycerides and inhibits secretion of very low-density lipoproteins","volume":"26","author":"Magnusson","year":"2006","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"37713","DOI":"10.1074\/jbc.M304025200","article-title":"Adipocyte protein S3-12 coats nascent lipid droplets","volume":"278","author":"Wolins","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1016\/j.bbalip.2017.07.009","article-title":"The perilipin family of lipid droplet proteins: Gatekeepers of intracellular lipolysis","volume":"1862","author":"Sztalryd","year":"2017","journal-title":"Biochim. Biophys. Acta. Mol. Cell Biol. Lipids"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"14232","DOI":"10.1074\/jbc.M601682200","article-title":"MLDP, a novel PAT family protein localized to lipid droplets and enriched in the heart, is regulated by peroxisome proliferator-activated receptor alpha","volume":"281","author":"Yamaguchi","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"E770","DOI":"10.1152\/ajpendo.00523.2012","article-title":"Inactivation of Plin4 downregulates Plin5 and reduces cardiac lipid accumulation in mice","volume":"304","author":"Chen","year":"2013","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.bbadis.2015.10.024","article-title":"Spontaneous hypertension occurs with adipose tissue dysfunction in perilipin-1 null mice","volume":"1862","author":"Zou","year":"2016","journal-title":"Biochim. Biophys. Acta Mol. Basis Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1093\/cvr\/cvu214","article-title":"Development of hypertrophic cardiomyopathy in perilipin-1 nullmice with adipose tissue dysfunction","volume":"105","author":"Liu","year":"2015","journal-title":"Cardiovasc. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1111\/his.12038","article-title":"Adipophilin\/perilipin-2 as a lipid droplet-specific marker for metabolically active cells and diseases associated with metabolic dysregulation","volume":"62","author":"Straub","year":"2013","journal-title":"Histopathology"},{"key":"ref_16","first-page":"631","article-title":"Lipid droplet associated proteins: An emerging role in atherogenesis","volume":"26","author":"Buers","year":"2011","journal-title":"Histol. Histopathol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1194\/jlr.M034710","article-title":"Haemmerle, cardiac-specific overexpression of perilipin 5 provokes severe cardiac steatosis via the formation of a lipolytic barrier","volume":"54","author":"Pollak","year":"2013","journal-title":"J. Lipid Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.yexcr.2015.10.031","article-title":"Heart lipid droplets and lipid droplet-binding proteins: Biochemistry, physiology, and pathology","volume":"340","author":"Osumi","year":"2016","journal-title":"Exp. Cell Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1038\/nrm1983","article-title":"Regulation of cardiac hypertrophy by intracellular signalling pathways","volume":"7","author":"Heineke","year":"2006","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1038\/s41569-018-0007-y","article-title":"Mechanisms of physiological and pathological cardiac hypertrophy","volume":"15","author":"Nakamura","year":"2018","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1016\/j.yjmcc.2009.01.013","article-title":"Stimulus specific changes of energy metabolism in hypertrophied heart","volume":"46","author":"Rimbaud","year":"2009","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1023\/A:1015332726303","article-title":"Gene regulatory mechanisms governing energy metabolism during cardiac hypertrophic growth","volume":"7","author":"Lehman","year":"2002","journal-title":"Heart Fail. Rev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1161\/CIRCRESAHA.118.312017","article-title":"Metabolic coordination of physiological and pathological cardiac remodeling","volume":"123","author":"Gibb","year":"2018","journal-title":"Circ. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1113\/expphysiol.2014.084434","article-title":"Changes in mitochondrial perilipin 3 and perilipin 5 protein content in rat skeletal muscle following endurance training and acute stimulated contraction","volume":"100","author":"Ramos","year":"2015","journal-title":"Exp. Physiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.1161\/CIRCRESAHA.117.311456","article-title":"PDK4 inhibits cardiac pyruvate oxidation in late pregnancy","volume":"121","author":"Liu","year":"2017","journal-title":"Circ. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"12723","DOI":"10.1038\/ncomms12723","article-title":"Nuclear perilipin 5 integrates lipid droplet lipolysis with PGC-1\u03b1\/SIRT1-dependent transcriptional regulation of mitochondrial function","volume":"7","author":"Saxena","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1016\/j.molcel.2019.12.003","article-title":"Lipid droplet-derived monounsaturated fatty acids traffic via PLIN5 to allosterically activate SIRT1","volume":"77","author":"Najt","year":"2019","journal-title":"Mol. Cell"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.gene.2016.06.025","article-title":"HIF-1\u03b1 and PPAR\u03b3 during physiological cardiac hypertrophy induced by pregnancy: Transcriptional activities and effects on target genes","volume":"591","author":"Ortiz","year":"2016","journal-title":"Gene"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1177\/0192623315570339","article-title":"Vaginal cytology of the laboratory rat and mouse: Review and criteria for the staging of the estrous cycle using stained vaginal smears","volume":"43","author":"Cora","year":"2015","journal-title":"Toxicol. Pathol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40738-020-00074-3","article-title":"Staging of the estrous cycle and induction of estrus in experimental rodents: An update","volume":"6","author":"Ajayi","year":"2020","journal-title":"Fertil. Res. Pract."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/j.bbrc.2017.06.087","article-title":"Betaine aldehyde dehydrogenase expression during physiological cardiac hypertrophy induced by pregnancy","volume":"490","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"101119","DOI":"10.1016\/j.redox.2019.101119","article-title":"Lipoxidation in cardiovascular diseases","volume":"23","author":"Gianazza","year":"2019","journal-title":"Redox Biol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Nose, F., Yamaguchi, T., Kato, R., Aiuchi, T., Obama, T., Hara, S., Yamamoto, M., and Itabe, H. (2013). Crucial role of perilipin-3 (TIP47) in formation of lipid droplets and PGE2 production in HL-60-derived neutrophils. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0071542"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"791","DOI":"10.2337\/db17-0983","article-title":"Perilipin 3 deficiency stimulates thermogenic beige adipocytes through PPAR\u03b1 activation","volume":"67","author":"Lee","year":"2018","journal-title":"Diabetes"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3683","DOI":"10.1210\/JC.2014-4125","article-title":"Perilipin 3 differentially regulates skeletal muscle lipid oxidation in active, sedentary, and type 2 diabetic males","volume":"100","author":"Covington","year":"2015","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1194\/jlr.M032466","article-title":"Cardiomyocyte-specific perilipin 5 overexpression leads to myocardial steatosis and modest cardiac dysfunction","volume":"54","author":"Wang","year":"2013","journal-title":"J. Lipid Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1194\/jlr.M043471","article-title":"Lipoprotein lipase activity is required for cardiac lipid droplet production","volume":"55","author":"Trent","year":"2014","journal-title":"J. Lipid Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s00441-018-2845-7","article-title":"Widespread expression of perilipin 5 in normal human tissues and in diseases is restricted to distinct lipid droplet subpopulations","volume":"374","author":"Hashani","year":"2018","journal-title":"Cell Tissue Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.bbalip.2006.11.011","article-title":"LSDP5 is a PAT protein specifically expressed in fatty acid oxidizing tissues","volume":"1771","author":"Dalen","year":"2007","journal-title":"Biochim. Biophys. Acta Mol. Cell Biol. Lipids"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2721","DOI":"10.1128\/MCB.00133-14","article-title":"Deficiency of a lipid droplet protein, perilipin 5, suppresses myocardial lipid accumulation, thereby preventing type 1 diabetes-induced heart malfunction","volume":"34","author":"Kuramoto","year":"2014","journal-title":"Mol. Cell. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"23852","DOI":"10.1074\/jbc.M111.328708","article-title":"Perilipin 5, a lipid droplet-binding protein, protects heart from oxidative burden by sequestering fatty acid from excessive oxidation","volume":"287","author":"Kuramoto","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1097\/MOL.0000000000000057","article-title":"Perilipin 5, a lipid droplet protein adapted to mitochondrial energy utilization","volume":"25","author":"Kimmel","year":"2014","journal-title":"Curr. Opin. Lipidol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Virgen-Ortiz, A., Lim\u00f3n-Miranda, S., Salazar-Enr\u00edquez, D.G., Melnikov, V., S\u00e1nchez-Pastor, E.A., and Castro-Rodr\u00edguez, E.M. (2019). Matrix metalloproteinases system and types of fibrosis in rat heart during late pregnancy and postpartum. Medicina, 55.","DOI":"10.3390\/medicina55050199"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1093\/cvr\/cvt208","article-title":"Calcineurin activity is required for cardiac remodelling in pregnancy","volume":"100","author":"Chung","year":"2013","journal-title":"Cardiovasc. Res."}],"container-title":["Medicina"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1648-9144\/58\/10\/1433\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:50:00Z","timestamp":1760143800000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1648-9144\/58\/10\/1433"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,11]]},"references-count":44,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["medicina58101433"],"URL":"https:\/\/doi.org\/10.3390\/medicina58101433","relation":{},"ISSN":["1648-9144"],"issn-type":[{"type":"electronic","value":"1648-9144"}],"subject":[],"published":{"date-parts":[[2022,10,11]]}}}