{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T03:41:45Z","timestamp":1768275705804,"version":"3.49.0"},"reference-count":27,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T00:00:00Z","timestamp":1665705600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hainan Provincial Natural Science Foundation of China","award":["820CXTD438"],"award-info":[{"award-number":["820CXTD438"]}]},{"name":"Hainan Provincial Natural Science Foundation of China","award":["82160634"],"award-info":[{"award-number":["82160634"]}]},{"name":"Hainan Provincial Natural Science Foundation of China","award":["81773495"],"award-info":[{"award-number":["81773495"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["820CXTD438"],"award-info":[{"award-number":["820CXTD438"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82160634"],"award-info":[{"award-number":["82160634"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81773495"],"award-info":[{"award-number":["81773495"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Hainan Province Clinical Medical Center","award":["820CXTD438"],"award-info":[{"award-number":["820CXTD438"]}]},{"name":"Hainan Province Clinical Medical Center","award":["82160634"],"award-info":[{"award-number":["82160634"]}]},{"name":"Hainan Province Clinical Medical Center","award":["81773495"],"award-info":[{"award-number":["81773495"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and Objectives: The role of \u03b1-enolase (ENO1) in Helicobacter pylori-related gastric lesions might be a critical factor in the pathogenesis, but remains undefined. Materials and Methods: This study investigated the differential expression of \u03b1-enolase in clinical gastric specimens and cultured normal\/cancer cells in response to H. pylori (cagA+) infection and cagA transfection using qPCR, Western blots and histochemical methods. Results: A total of 172 gastric specimens were collected from 142 patients, the former comprising chronic superficial gastritis (CSG), precancerous diseases (PCDs, including atrophic gastritis, intestinal metaplasia and dysplasia) and gastric cancer (GC) cases. Among the CSG and PCD cases, the H. pylori-infected group had significantly elevated ENO1 mRNA levels compared with the uninfected group. In the GC cases, differential ENO1 expressions were detected between the cancer tissues and the paracancerous tissues. Notably, significant difference was first detected between the GC cell (AGS) and the normal cell (GES-1) as a response of ENO1 to H. pylori infection and cagA transfection. Conclusions: This report reveals that ENO1 expression is associated with H. pylori infection, cagA transfection, co-culture duration, multiplicity of infection, gastric normal\/cancerous cell lines and cellular differentiation. The findings may be crucial bases for further ascertaining H. pylori pathogenic mechanism and formulating novel therapeutic and diagnostic strategies.<\/jats:p>","DOI":"10.3390\/medicina58101453","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T02:29:46Z","timestamp":1665973786000},"page":"1453","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Differential Expression of \u03b1-Enolase in Clinical Gastric Tissues and Cultured Normal\/Cancer Cells in Response to Helicobacter pylori Infection and cagA Transfection"],"prefix":"10.3390","volume":"58","author":[{"given":"Feiyan","family":"Yu","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital, International School of Public Health and One Health, Hainan Medical University, Haikou 570102, China"},{"name":"Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengya","family":"He","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Li","sequence":"additional","affiliation":[{"name":"Henan Provincial People\u2019s Hospital, Zhengzhou University, Zhengzhou 450003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6129-0310","authenticated-orcid":false,"given":"Shuaiyin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Henan University of Science and Technology, Luoyang 471000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huijuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, International School of Public Health and One Health, Hainan Medical University, Haikou 570102, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangcai","family":"Duan","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, International School of Public Health and One Health, Hainan Medical University, Haikou 570102, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9961-2434","authenticated-orcid":false,"given":"Rongguang","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, International School of Public Health and One Health, Hainan Medical University, Haikou 570102, China"},{"name":"Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Machlowska, J., Baj, J., Sitarz, M., Maciejewski, R., and Sitarz, R. 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