{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T08:13:50Z","timestamp":1774340030032,"version":"3.50.1"},"reference-count":50,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,10,20]],"date-time":"2022-10-20T00:00:00Z","timestamp":1666224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Background and objectives: Preimplantation genetic testing (PGT) offers patients the possibility of having a healthy baby free of chromosomal or genetic disorders. The present study focuses on the application of PGT for patients located in Northern Greece, investigating their clinical outcomes, their motives, and their overall physical and emotional experience during the treatment, in association with their socioeconomic background. Materials and Methods: Couples who underwent PGT for a monogenic condition (PGT-M, n = 19 cycles) or aneuploidy (PGT-A, n = 22 cycles) participated in the study. Fertilization, implantation, and pregnancy rates were recorded for all cycles. The couples were asked to fill in a questionnaire about the consultation they had received prior to treatment, their sociodemographic information, and the psychological impact PGT had on both the female and male partner. Results: The fertilization, implantation, and ongoing pregnancy rates for the PGT-M and PGT-A cycles were 81.3%, 70.6%, and 52.9%, and 78.2%, 64.3%, and 57.1%, respectively. Females experienced more intense physical pain than their male partners while psychological pain was encountered by both partners and occasionally in higher instances in males. No typical socioeconomic background of the patients referred for PGT in Northern Greece was noticed. Conclusion: PGT is an attractive alternative to prenatal diagnosis (PND), aiming to establisha healthy pregnancy by identifying and avoiding the transfer of chromosomally or genetically abnormal embryos to the uterus. Although the benefits of PGT were well-received by all patients undergoing the procedure, psychological pain was evident and especially prominent in patients with a previous affected child or no normal embryos for transfer. Holistic counseling is of utmost importance in order to make patients\u2019 experience during their journey to have a healthy baby less emotionally demanding and help them make the right choices for the future.<\/jats:p>","DOI":"10.3390\/medicina58101493","type":"journal-article","created":{"date-parts":[[2022,10,20]],"date-time":"2022-10-20T00:43:52Z","timestamp":1666226632000},"page":"1493","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Clinical Outcome, Socioeconomic Status and Psychological Constrains of Patients Undergoing Preimplantation Genetic Testing (PGT) in Northern Greece"],"prefix":"10.3390","volume":"58","author":[{"given":"Antonios","family":"Patrikiou","sequence":"first","affiliation":[{"name":"Unit for Human Reproduction, 1st Department of Obstetrics & Gynaecology, Aristotle University Medical School, Papageorgiou General Hospital, 56403 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8958-2374","authenticated-orcid":false,"given":"Alexandra","family":"Papadopoulou","sequence":"additional","affiliation":[{"name":"Unit for Human Reproduction, 1st Department of Obstetrics & Gynaecology, Aristotle University Medical School, Papageorgiou General Hospital, 56403 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1570-4724","authenticated-orcid":false,"given":"Christos","family":"Noutsos","sequence":"additional","affiliation":[{"name":"Biology Department, SUNY College at Old Westbury, Old Westbury, NY 11731, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7519-8811","authenticated-orcid":false,"given":"Panagiotis","family":"Tzekis","sequence":"additional","affiliation":[{"name":"Department of Information and Electronic Engineering, International Hellenic University, Thermi, 57001 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolaos","family":"Koios","sequence":"additional","affiliation":[{"name":"University Ecclesiastical Academy of Thessaloniki, 54250 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ioannis","family":"Kourempeles","sequence":"additional","affiliation":[{"name":"Dogmatics Theology, School of Theology, Faculty of Theology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Anifandis","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, School of Health Sciences, Faculty of Medicine, University of Thessaly, 41200 Larisa, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7565-2025","authenticated-orcid":false,"given":"Antonia","family":"Sioga","sequence":"additional","affiliation":[{"name":"Laboratory of Histology and Embryology, Aristotle University Medical School, 54124 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grigoris","family":"Grimbizis","sequence":"additional","affiliation":[{"name":"Unit for Human Reproduction, 1st Department of Obstetrics & Gynaecology, Aristotle University Medical School, Papageorgiou General Hospital, 56403 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Basil C.","family":"Tarlatzis","sequence":"additional","affiliation":[{"name":"Unit for Human Reproduction, 1st Department of Obstetrics & Gynaecology, Aristotle University Medical School, Papageorgiou General Hospital, 56403 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9624-6352","authenticated-orcid":false,"given":"Katerina","family":"Chatzimeletiou","sequence":"additional","affiliation":[{"name":"Unit for Human Reproduction, 1st Department of Obstetrics & Gynaecology, Aristotle University Medical School, Papageorgiou General Hospital, 56403 Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1038\/229132a0","article-title":"Human blastocysts grown in culture","volume":"229","author":"Steptoe","year":"1971","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1093\/humrep\/deu033","article-title":"Correlation between standard blastocyst morphology, euploidy and implantation: An observational study in two centers involving 956 screened blastocysts","volume":"29","author":"Capalbo","year":"2014","journal-title":"Hum. Reprod."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/bs.ctdb.2016.04.009","article-title":"Genetic Analysis of Human Preimplantation Embryos","volume":"120","author":"Cervero","year":"2016","journal-title":"Curr. Top. Dev. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1093\/humrep\/deh652","article-title":"Spindle abnormalities in normally developing and arrested human preimplantation embryos in vitro identified by confocal laser scanning microscopy","volume":"20","author":"Chatzimeletiou","year":"2005","journal-title":"Hum. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/S1472-6483(10)60455-5","article-title":"Symposium: Genetic and epigenetic aspects of assisted reproduction. The centrosome and early embryogenesis: Clinical insights","volume":"16","author":"Chatzimeletiou","year":"2008","journal-title":"Reprod. Biomed. Online"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1017\/S0967199406004059","article-title":"Is the sperm centrosome to blame for the complex polyploid chromosome patterns observed in cleavage stage embryos from an OAT patient?","volume":"15","author":"Chatzimeletiou","year":"2007","journal-title":"Zygote"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1093\/hmg\/ddy147","article-title":"Tripolar chromosome segregation drives the association between maternal genotype at variants spanning PLK4 and aneuploidy in human preimplantation embryos","volume":"27","author":"McCoy","year":"2018","journal-title":"Hum. Mol. Genet."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1002\/pd.1227","article-title":"Aneuploidy screening in coelomic samples using fluorescence in situ hybridisation (FISH)","volume":"25","author":"Chatzimeletiou","year":"2005","journal-title":"Prenat. Diagn."},{"key":"ref_9","first-page":"1071","article-title":"STAR Study Group. Preimplantation genetic testing for aneuploidy versus morphology as selection criteria for single frozen-thawed embryo transfer in good-prognosis patients: A multicenter randomized clinical trial","volume":"112","author":"Kaplan","year":"2019","journal-title":"FertilSteril"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1093\/humrep\/deaa034","article-title":"The status of preimplantation genetic testing in the UK and USA","volume":"35","author":"Theobald","year":"2020","journal-title":"Hum. Reprod."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"ESHRE PGT Consortium Steering Committee, Carvalho, F., Coonen, E., Goossens, V., Kokkali, G., Rubio, C., Meijer-Hoogeveen, M., Moutou, C., Vermeulen, N., and De Rycke, M. (2020). ESHRE PGT Consortium good practice recommendations for the organisation of PGT. Hum. Reprod. Open., 2020, hoaa021.","DOI":"10.1093\/hropen\/hoaa021"},{"key":"ref_12","first-page":"e1","article-title":"Semen analysis by electron and fluorescence microscopy in a case of partial hydatidiform mole reveals a high incidence of abnormal morphology, diploidy, and tetraploidy","volume":"95","author":"Chatzimeletiou","year":"2011","journal-title":"FertilSteril"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1016\/j.rbmo.2021.05.011","article-title":"Viability assessment using fluorescent markers and ultrastructure of human biopsied embryos vitrified in open and closed systems","volume":"43","author":"Chatzimeletiou","year":"2021","journal-title":"Reprod. Biomed. Online"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Chatzimeletiou, K., Petrogiannis, N., Sioga, A., Emmanouil-Nikoloussi, E.N., Panagiotidis, Y., Prapa, M., Patrikiou, A., Filippa, M., Zervakakou, G., and Papanikolaou, K. (RBMO, 2022). The human embryo following biopsy on day 5 vs day 3: Viability ultrastructure and spindle chromosome configurations, RBMO, online ahead of print.","DOI":"10.1016\/j.rbmo.2022.02.022"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2488","DOI":"10.1093\/humrep\/deaa236","article-title":"Multiple vitrification-warming and biopsy procedures on human embryos: Clinical outcome and neonatal follow-up of children","volume":"35","author":"Verdyck","year":"2020","journal-title":"Hum. Reprod."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1038\/344768a0","article-title":"Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification","volume":"344","author":"Handyside","year":"1990","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1038\/s10038-020-0732-z","article-title":"Preimplantation genetic testing with HLA matching: From counseling to birth and beyond","volume":"65","author":"Belva","year":"2020","journal-title":"J. Hum. Genet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1093\/humrep\/17.3.534","article-title":"Ethical considerations on preimplantation genetic diagnosis for HLA typing to match a future child as a donor of haematopoietic stem cells to a sibling","volume":"17","author":"Pennings","year":"2002","journal-title":"Hum. Reprod."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.bpobgyn.2017.05.008","article-title":"Pre-implantation HLA matching: The production of a Saviour Child","volume":"44","author":"Kakourou","year":"2017","journal-title":"Best Pract. Res. Clin. Obstet Gynaecol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/S1472-6483(10)61688-4","article-title":"Comparison of effects of zona drilling by non contact infrared laser or acid Tyrode\u2019s on the development of human biopsied embryos as revealed by blastomere viability, cytoskeletal analysis and molecular cytogenetics","volume":"11","author":"Chatzimeletiou","year":"2005","journal-title":"Reprod. Biomed. Online"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1093\/oxfordjournals.humrep.a137288","article-title":"Human trophectoderm biopsy and secretion of chorionic gonadotrophin","volume":"6","author":"Dokras","year":"1991","journal-title":"Hum. Reprod."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7193075","DOI":"10.1155\/2016\/7193075","article-title":"The Impact of Biopsy on Human Embryo Developmental Potential during Preimplantation Genetic Diagnosis","volume":"2016","author":"Cimadomo","year":"2016","journal-title":"Biomed. Res. Int."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.5114\/aoms.2018.72790","article-title":"What should we focus on before preimplantation genetic diagnosis\/screening?","volume":"14","author":"Zheng","year":"2018","journal-title":"Arch. Med. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/aogs.13973","article-title":"A systematic review exploring the patient decision-making factors and attitudes towards pre-implantation genetic testing for aneuploidy and gender selection","volume":"100","author":"Saso","year":"2021","journal-title":"Acta Obstet Gynecol. Scand."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1093\/humupd\/dmab013","article-title":"review on the motivations, decision-making factors, attitudes and experiences of couples using pre-implantation genetic testing for inherited conditions","volume":"27","author":"Hughes","year":"2021","journal-title":"Hum. Rep. Update"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1007\/s10815-020-01840-4","article-title":"The decision-making process, experience, and perceptions of preimplantation genetic testing (PGT) users","volume":"37","author":"Zuckerman","year":"2020","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3272","DOI":"10.1007\/s43032-021-00644-w","article-title":"Comparison of Patients\u2019 Ethical Perspectives of Preimplantation Embryo Genetic Testing for Aneuploidy (PGT-A) vs. Monogenic Disorders (PGT-M)","volume":"28","author":"Zhang","year":"2021","journal-title":"Reprod. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2464","DOI":"10.1093\/humrep\/17.9.2464","article-title":"Preimplantation genetic diagnosis: Patients\u2019 experiences and attitudes","volume":"17","author":"Lavery","year":"2002","journal-title":"Hum. Reprod."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.rbmo.2009.10.005","article-title":"Psychological impact of preimplantation genetic diagnosis: A review of the literature","volume":"20","author":"Karatas","year":"2010","journal-title":"Reprod. Biomed. Online"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Aimagambetova, G., Issanov, A., Terzic, S., Bapayeva, G., Ukybassova, T., Baikoshkarova, S., Aldiyarova, A., Shauyen, F., and Terzic, M. (2020). The effect of psychological distress on IVF outcomes: Reality or speculations?. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0242024"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3126","DOI":"10.1093\/humrep\/14.12.3126","article-title":"Anxiety and perceptive functioning of infertile women during in-vitro fertilization: Exploratory survey of an Italian sample","volume":"14","author":"Ardenti","year":"1999","journal-title":"Hum. Reprod."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.ejogrb.2014.10.019","article-title":"Depression and coping strategies of Chinese women undergoing in-vitro fertilization","volume":"183","author":"Wu","year":"2014","journal-title":"Eur. J. Obstet Gynecol. Reprod. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/humupd\/dml040","article-title":"Women\u2019s emotional adjustment to IVF: A systematic review of 25 years of research","volume":"13","author":"Verhaak","year":"2017","journal-title":"Hum. Reprod. Update"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1007\/s10815-018-1278-2","article-title":"Pre-implantation genetic testing: Decisional factors to accept or decline among in vitro fertilization patients","volume":"35","author":"Lamb","year":"2018","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Maroufizadeh, S., Navid, B., Omani-Samani, R., and Amini, P. (2019). The effects of depression, anxiety and stress symptoms on the clinical pregnancy rate in women undergoing IVF treatment. BMC Res. Notes, 12.","DOI":"10.1186\/s13104-019-4294-0"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e2017018","DOI":"10.4084\/mjhid.2017.018","article-title":"\u03b2-Thalassemia Distribution in the Old World: An Ancient Disease Seen from a Historical Standpoint","volume":"9","author":"Kattamis","year":"2017","journal-title":"Mediterr. J. Hematol. Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1093\/emph\/eoz034","article-title":"Hemoglobin E, malaria and natural selection","volume":"13","author":"Ha","year":"2019","journal-title":"Evol. Med. Public Health"},{"key":"ref_38","first-page":"277","article-title":"Gestational surrogacy. Ethical aspects","volume":"3","author":"Aznar","year":"2018","journal-title":"Med. Morale."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1177\/0024363919830840","article-title":"Gestational Surrogacy: Current View","volume":"861","author":"Aznar","year":"2019","journal-title":"Linacre Q."},{"key":"ref_40","unstructured":"Vodo, T. (2020). Altruistic Surrogacy: Why to oppose empathetic gestures?. Eur. Christ. Political Mov. White Pap., 1\u201318."},{"key":"ref_41","first-page":"43","article-title":"Psychological Aspects of Surrogate Motherhood","volume":"9","author":"Shafiabadi","year":"2008","journal-title":"J. Reprod. Infertil."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1017\/S0967199419000182","article-title":"Spindle and chromosome configuration analysis of human biopsied versus non-biopsied embryos by confocal laser scanning microscopy following vitrification","volume":"27","author":"Chatzimeletiou","year":"2019","journal-title":"Zygote"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1093\/humrep\/der344","article-title":"Cytoskeletal analysis of human blastocysts by confocal laser scanning microscopy following vitrification","volume":"27","author":"Chatzimeletiou","year":"2012","journal-title":"Hum. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1111\/cge.13732","article-title":"PGT-A preimplantation genetic testing for aneuploidies and embryo selection in routine ART cycles: Time to step back?","volume":"98","author":"Sciorio","year":"2020","journal-title":"Clin. Genet."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.ajog.2020.04.035","article-title":"Multicenter prospective study of concordance between embryonic cell-free DNA and trophectoderm biopsies from 1301 human blastocysts","volume":"223","author":"Rubio","year":"2020","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_46","first-page":"280","article-title":"One hundred mosaic embryos transferred prospectively in a single clinic: Exploring when and why they result in healthy pregnancies","volume":"111","author":"Victor","year":"2019","journal-title":"FertilSteril"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2238","DOI":"10.1016\/j.ajhg.2021.11.002","article-title":"Mosaic human preimplantation embryos and their developmental potential in a prospective, non-selection clinical trial","volume":"108","author":"Capalbo","year":"2021","journal-title":"Am. J. Hum. Genet."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/BF01203757","article-title":"Psychological and hormonal changes in the course of in vitro fertilization","volume":"9","author":"Merari","year":"1992","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1080\/19396368.2022.2052771","article-title":"Bioethics in human embryology: The double-edged sword of embryo research","volume":"68","author":"Anifandis","year":"2022","journal-title":"Syst. Biol. Reprod. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1007\/s10815-022-02439-7","article-title":"Revisiting selected ethical aspects of current clinical in vitro fertilization (IVF) practice","volume":"39","author":"Mochizuki","year":"2022","journal-title":"J. Assist. Reprod. Genet."}],"container-title":["Medicina"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1648-9144\/58\/10\/1493\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:57:44Z","timestamp":1760144264000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1648-9144\/58\/10\/1493"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,20]]},"references-count":50,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["medicina58101493"],"URL":"https:\/\/doi.org\/10.3390\/medicina58101493","relation":{},"ISSN":["1648-9144"],"issn-type":[{"value":"1648-9144","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,20]]}}}