{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T11:52:23Z","timestamp":1774957943791,"version":"3.50.1"},"reference-count":30,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T00:00:00Z","timestamp":1671062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"university internal funds"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Medicina"],"abstract":"<jats:p>Hypertrophic obstructive cardiomyopathy (HOCM) is one of the most common hereditary heart diseases. The severely hypertrophied interventricular septum combined with the systolic anterior movement (SAM) of the mitral valve (MV) frequently cause a significant pressure gradient in the left ventricular outflow tract associated with varying degrees of mitral regurgitation (MR). We present the case of a 64-year-old female patient who was diagnosed with HOCM two years ago and was admitted to the Institute of Cardiovascular Disease with exertion dyspnea and fatigue. Transthoracic echocardiography revealed concentric, asymmetrical left ventricular hypertrophy, an elongated anterior mitral leaflet (AML) and a significant SAM causing severe regurgitation, with indication for valvular replacement Monoamine oxidase (MAO), a mitochondrial enzyme, with 2 isoforms, MAO-A and B, has emerged as an important source of reactive oxygen species (ROS) in the cardiovascular system, but literature data on its expression in valvular tissue is scarce. Therefore, we assessed MAO-A and B gene (qPCR) and protein (immune fluorescence) expression as well as ROS production (spectrophotometry and confocal microscopy) and in the explanted MV harvested during replacement surgery. MAO expression and ROS production (assessed by both methods) were further augmented following ex vivo incubation with angiotensin II, an effect that was reversed in the presence of either MAO-A (clorgyline) or B (selegiline) inhibitor, respectively. In conclusion, MAO isoforms are expressed at the level of severely impaired mitral valve in the setting of HOCM and can be induced in conditions that mimic the activation of renin-angiotensin-aldosterone system. The observation that the enzyme can be modulated by MAO inhibitors warrants further investigation in a patient cohort.<\/jats:p>","DOI":"10.3390\/medicina58121844","type":"journal-article","created":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T04:54:14Z","timestamp":1671080054000},"page":"1844","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Monoamine Oxidase (MAO) Is Expressed at the Level of Mitral Valve with Severe Regurgitation in Hypertrophic Obstructive Cardiomyopathy: A Case Report"],"prefix":"10.3390","volume":"58","author":[{"given":"Ana","family":"Lascu","sequence":"first","affiliation":[{"name":"Department III\u2014Pathophysiology, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy of Timi\u0219oara, E. Murgu Sq. No. 2, 300041 Timi\u0219oara, Romania"},{"name":"Center for Translational Research and Systems Medicine, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy of Timi\u0219oara, E. Murgu Sq. No. 2, 300041 Timi\u0219oara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5998-2932","authenticated-orcid":false,"given":"Raluca","family":"\u0218o\u0219dean","sequence":"additional","affiliation":[{"name":"Department VI\u2014Cardiology, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy of Timi\u0219oara, E. Murgu Sq. No. 2, 300041 Timi\u0219oara, Romania"},{"name":"Research Center of the Institute for Cardiovascular Diseases, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy from Timi\u0219oara, G. Adam St. No. 13A, 300310 Timi\u0219oara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loredana Nicoleta","family":"Ionic\u0103","sequence":"additional","affiliation":[{"name":"Center for Translational Research and Systems Medicine, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy of Timi\u0219oara, E. Murgu Sq. No. 2, 300041 Timi\u0219oara, Romania"},{"name":"Doctoral School Medicine-Pharmacy, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy Timi\u0219oara, E. Murgu Sq. No. 2, 300041 Timi\u0219oara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1697-311X","authenticated-orcid":false,"given":"Alexandru S.","family":"Pescariu","sequence":"additional","affiliation":[{"name":"Department VI\u2014Cardiology, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy of Timi\u0219oara, E. Murgu Sq. No. 2, 300041 Timi\u0219oara, Romania"},{"name":"Research Center of the Institute for Cardiovascular Diseases, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy from Timi\u0219oara, G. Adam St. No. 13A, 300310 Timi\u0219oara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucian","family":"Petrescu","sequence":"additional","affiliation":[{"name":"Center for Translational Research and Systems Medicine, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy of Timi\u0219oara, E. Murgu Sq. No. 2, 300041 Timi\u0219oara, Romania"},{"name":"Doctoral School Medicine-Pharmacy, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy Timi\u0219oara, E. Murgu Sq. No. 2, 300041 Timi\u0219oara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adina","family":"Ionac","sequence":"additional","affiliation":[{"name":"Department VI\u2014Cardiology, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy of Timi\u0219oara, E. Murgu Sq. No. 2, 300041 Timi\u0219oara, Romania"},{"name":"Research Center of the Institute for Cardiovascular Diseases, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy from Timi\u0219oara, G. Adam St. No. 13A, 300310 Timi\u0219oara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constantin T.","family":"Luca","sequence":"additional","affiliation":[{"name":"Department VI\u2014Cardiology, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy of Timi\u0219oara, E. Murgu Sq. No. 2, 300041 Timi\u0219oara, Romania"},{"name":"Research Center of the Institute for Cardiovascular Diseases, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy from Timi\u0219oara, G. Adam St. No. 13A, 300310 Timi\u0219oara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian","family":"Sturza","sequence":"additional","affiliation":[{"name":"Department III\u2014Pathophysiology, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy of Timi\u0219oara, E. Murgu Sq. No. 2, 300041 Timi\u0219oara, Romania"},{"name":"Center for Translational Research and Systems Medicine, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy of Timi\u0219oara, E. Murgu Sq. No. 2, 300041 Timi\u0219oara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4870-8249","authenticated-orcid":false,"given":"Horea B.","family":"Feier","sequence":"additional","affiliation":[{"name":"Research Center of the Institute for Cardiovascular Diseases, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy from Timi\u0219oara, G. Adam St. No. 13A, 300310 Timi\u0219oara, Romania"},{"name":"Department VI\u2014Cardiovascular Surgery, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy of Timi\u0219oara, E. Murgu Sq. No. 2, 300041 Timi\u0219oara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1016\/j.jacc.2015.01.019","article-title":"New Perspectives on the Prevalence of Hypertrophic Cardiomyopathy","volume":"65","author":"Semsarian","year":"2015","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2733","DOI":"10.1093\/eurheartj\/ehu284","article-title":"2014 Esc Guidelines on Diagnosis and Management of Hypertrophic Cardiomyopathy: The Task Force for the Diagnosis and Management of Hypertrophic Cardiomyopathy of the European Society of Cardiology (Esc)","volume":"35","author":"Elliott","year":"2014","journal-title":"Eur. 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